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The map label for this gene is kefB [H]

Identifier: 88606860

GI number: 88606860

Start: 1371452

End: 1373167

Strand: Reverse

Name: kefB [H]

Synonym: APH_1305

Alternate gene names: 88606860

Gene position: 1373167-1371452 (Counterclockwise)

Preceding gene: 88607529

Following gene: 88607238

Centisome position: 93.33

GC content: 45.8

Gene sequence:

>1716_bases
ATGCACCATTCTAGTTATCTGTTGGATGTCATAGTGCTATTAGCTGCTGCGGTAGTGGTGGTGGTGCTGTTCTGGAAGTT
GCGTATCAGTCCTGTGCTTGGATATTTTGTTGCGGGAGCTGCTATTGGTACGCATGGGCTGCAACTAGTTGAATCTTCGT
CTATTATCAATAGTTTAGCTGAGTTTGGGGTTGTATTTCTTCTATTTTTGATAGGGATAGAGTTAACCTTTGAGCGACTG
TCGGCCATGCGAATGCATGTCTTTGGTTTTGGGACATTGCAGGTAATAGTTACGGCCACGTTGATATGGTGCGTGGCGCG
TGGGCTTGGTGTTGATTCCGAGAGCTCTGTGGTTATTGGGGGTGCACTTGCGCTTTCTTCCACTGCTATTGTGTTGCAGG
TATTGCAGGAGAAGGGTATGCAATCCTCGCAGGTTGGTAGATTGTCCATAGCTGTGCTGCTATTGCAAGACTTCGCTGTT
GTGCCTTTGATAGTGTTGCTACCGTTGCTGGCGGGCGAGTCTAATAGTCTCGTGGGGTCCTTATTTATTGCTTTCCTGAA
GGCTGGGGTAGCACTTGCTCTTATATTTGTTACGGGAAGGCTGTTGTTGAGGCCATTGTTCGGCACTATAGCTGCTATGA
AGAGTAATGAGGTATTTATTGCCTCTACGCTACTTATAGTGTTGGGAGCTGCTTTCGTTACTGAGAATTTTAATCTCTCT
ATGGCGCTTGGGGCTTTTGTATCTGGTTTGCTGGTTGCGGAAACGGAGTACCGCTACGACGTAGAGCAGGTTGTATTACC
ATTCAAGAAGTTGCTCTTGGGTCTGTTCTTCATGACTGTGGGCATGTCTATAGATACGGAACTGCTGTTGAGCAAGCTAC
CTGTTGTTGCGGCTTTGTCGCTTTCTTTGATGCTATTGAAGTCATTTATTGTTTGCGTCCTGTGTAGATGTTTTCGCTTC
TCGTTGTCTGCGGGTTTACAGGCGGGTCTATTGCTTTCACAGGGTGGTGAGTTTTCTTTCATATTATTTGGGTTGGCTGC
CGAAAAGAGCGTGTTGTCACATGATCTCGCGCAAGTGCTGATGATGGCCACTACGGTTACGATGGCATTTACGCCTCTTC
TAGCTACTCTAGGGAATTGGATATCTGGATTTCTGACAAAGAACAAGGTTTCTTACACTTCTTCGGCGCTGGTGTTAGAT
ACTAGCGATTTAGATCAGCACGTGATAGTGGTTGGATTTGGTAGGGTAGGTCGCCTTGTAGCTAAGGTTTTGATGGCAGA
ACATGTTAACTATATAGCCTCTGATATTCAGCCAAGTGTTGTTACTGAGGGGAGGAAGGAGGGGTTTCCTGTATACTTAG
GAGATCTCACGAAGTTAGAGGTGCTTACCGCTATGGGGGCTTCTCGTGCTACTGCGATTGCTATTGCGATAAATAATGAG
GTCACGGCGAAGAAGGTGATAGCGCTTGTTGCTAAAAACTTCCCTGATACGATAATTGCGGTGCGTATACCGGATTTAAG
TAATGCTGACATGTATAAAAAGCTGGGGGCTCATTATTTGATTCCTGAAACCTATGAAGCAGGTTTGCAGCTGGGTGGTA
TAGCTCTTAGTATCAGTGGGTTCAGTGATAACGCGATTTTATCTTTGCAGAATGCTTTTCGTATGGAAGATTATCAGCGT
CCGGTGAAAAAAGAAAGCTCGGATGACGTCCGATAG

Upstream 100 bases:

>100_bases
TCGCTACTACGGGTGGTGTGCCTGAAAATCAGAAGCCTAGTATTGGGTGTTCCATAAAGTGGCGTTGCGTTGATGATATT
TTAAGTTAGTTTGTTCTTCT

Downstream 100 bases:

>100_bases
ATATGCCTTCTGCTATACACTAGGGGCAAGCATTGCTATTAATTTAGTTTTGGTGCTTAGGTAATGTCTGTACTGTAGGG
AAGCGTTTGTACAGCGTTGT

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MHHSSYLLDVIVLLAAAVVVVVLFWKLRISPVLGYFVAGAAIGTHGLQLVESSSIINSLAEFGVVFLLFLIGIELTFERL
SAMRMHVFGFGTLQVIVTATLIWCVARGLGVDSESSVVIGGALALSSTAIVLQVLQEKGMQSSQVGRLSIAVLLLQDFAV
VPLIVLLPLLAGESNSLVGSLFIAFLKAGVALALIFVTGRLLLRPLFGTIAAMKSNEVFIASTLLIVLGAAFVTENFNLS
MALGAFVSGLLVAETEYRYDVEQVVLPFKKLLLGLFFMTVGMSIDTELLLSKLPVVAALSLSLMLLKSFIVCVLCRCFRF
SLSAGLQAGLLLSQGGEFSFILFGLAAEKSVLSHDLAQVLMMATTVTMAFTPLLATLGNWISGFLTKNKVSYTSSALVLD
TSDLDQHVIVVGFGRVGRLVAKVLMAEHVNYIASDIQPSVVTEGRKEGFPVYLGDLTKLEVLTAMGASRATAIAIAINNE
VTAKKVIALVAKNFPDTIIAVRIPDLSNADMYKKLGAHYLIPETYEAGLQLGGIALSISGFSDNAILSLQNAFRMEDYQR
PVKKESSDDVR

Sequences:

>Translated_571_residues
MHHSSYLLDVIVLLAAAVVVVVLFWKLRISPVLGYFVAGAAIGTHGLQLVESSSIINSLAEFGVVFLLFLIGIELTFERL
SAMRMHVFGFGTLQVIVTATLIWCVARGLGVDSESSVVIGGALALSSTAIVLQVLQEKGMQSSQVGRLSIAVLLLQDFAV
VPLIVLLPLLAGESNSLVGSLFIAFLKAGVALALIFVTGRLLLRPLFGTIAAMKSNEVFIASTLLIVLGAAFVTENFNLS
MALGAFVSGLLVAETEYRYDVEQVVLPFKKLLLGLFFMTVGMSIDTELLLSKLPVVAALSLSLMLLKSFIVCVLCRCFRF
SLSAGLQAGLLLSQGGEFSFILFGLAAEKSVLSHDLAQVLMMATTVTMAFTPLLATLGNWISGFLTKNKVSYTSSALVLD
TSDLDQHVIVVGFGRVGRLVAKVLMAEHVNYIASDIQPSVVTEGRKEGFPVYLGDLTKLEVLTAMGASRATAIAIAINNE
VTAKKVIALVAKNFPDTIIAVRIPDLSNADMYKKLGAHYLIPETYEAGLQLGGIALSISGFSDNAILSLQNAFRMEDYQR
PVKKESSDDVR
>Mature_571_residues
MHHSSYLLDVIVLLAAAVVVVVLFWKLRISPVLGYFVAGAAIGTHGLQLVESSSIINSLAEFGVVFLLFLIGIELTFERL
SAMRMHVFGFGTLQVIVTATLIWCVARGLGVDSESSVVIGGALALSSTAIVLQVLQEKGMQSSQVGRLSIAVLLLQDFAV
VPLIVLLPLLAGESNSLVGSLFIAFLKAGVALALIFVTGRLLLRPLFGTIAAMKSNEVFIASTLLIVLGAAFVTENFNLS
MALGAFVSGLLVAETEYRYDVEQVVLPFKKLLLGLFFMTVGMSIDTELLLSKLPVVAALSLSLMLLKSFIVCVLCRCFRF
SLSAGLQAGLLLSQGGEFSFILFGLAAEKSVLSHDLAQVLMMATTVTMAFTPLLATLGNWISGFLTKNKVSYTSSALVLD
TSDLDQHVIVVGFGRVGRLVAKVLMAEHVNYIASDIQPSVVTEGRKEGFPVYLGDLTKLEVLTAMGASRATAIAIAINNE
VTAKKVIALVAKNFPDTIIAVRIPDLSNADMYKKLGAHYLIPETYEAGLQLGGIALSISGFSDNAILSLQNAFRMEDYQR
PVKKESSDDVR

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=375, Percent_Identity=25.6, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1786232, Length=558, Percent_Identity=35.3046594982079, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1789749, Length=563, Percent_Identity=36.9449378330373, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1786685, Length=518, Percent_Identity=28.957528957529, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 61150; Mature: 61150

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHSSYLLDVIVLLAAAVVVVVLFWKLRISPVLGYFVAGAAIGTHGLQLVESSSIINSLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFGVVFLLFLIGIELTFERLSAMRMHVFGFGTLQVIVTATLIWCVARGLGVDSESSVVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
GALALSSTAIVLQVLQEKGMQSSQVGRLSIAVLLLQDFAVVPLIVLLPLLAGESNSLVGS
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LFIAFLKAGVALALIFVTGRLLLRPLFGTIAAMKSNEVFIASTLLIVLGAAFVTENFNLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
MALGAFVSGLLVAETEYRYDVEQVVLPFKKLLLGLFFMTVGMSIDTELLLSKLPVVAALS
HHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHH
LSLMLLKSFIVCVLCRCFRFSLSAGLQAGLLLSQGGEFSFILFGLAAEKSVLSHDLAQVL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEECCCCEEEEEEEHHHHHHHHHHHHHHHH
MMATTVTMAFTPLLATLGNWISGFLTKNKVSYTSSALVLDTSDLDQHVIVVGFGRVGRLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCHHHHHH
AKVLMAEHVNYIASDIQPSVVTEGRKEGFPVYLGDLTKLEVLTAMGASRATAIAIAINNE
HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEECCHHHHHHHHHCCCCCCEEEEEEECCC
VTAKKVIALVAKNFPDTIIAVRIPDLSNADMYKKLGAHYLIPETYEAGLQLGGIALSISG
HHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCHHHCCCEECCEEEEEEC
FSDNAILSLQNAFRMEDYQRPVKKESSDDVR
CCCCCEEEHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHHSSYLLDVIVLLAAAVVVVVLFWKLRISPVLGYFVAGAAIGTHGLQLVESSSIINSLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFGVVFLLFLIGIELTFERLSAMRMHVFGFGTLQVIVTATLIWCVARGLGVDSESSVVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
GALALSSTAIVLQVLQEKGMQSSQVGRLSIAVLLLQDFAVVPLIVLLPLLAGESNSLVGS
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LFIAFLKAGVALALIFVTGRLLLRPLFGTIAAMKSNEVFIASTLLIVLGAAFVTENFNLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
MALGAFVSGLLVAETEYRYDVEQVVLPFKKLLLGLFFMTVGMSIDTELLLSKLPVVAALS
HHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHH
LSLMLLKSFIVCVLCRCFRFSLSAGLQAGLLLSQGGEFSFILFGLAAEKSVLSHDLAQVL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEECCCCEEEEEEEHHHHHHHHHHHHHHHH
MMATTVTMAFTPLLATLGNWISGFLTKNKVSYTSSALVLDTSDLDQHVIVVGFGRVGRLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCHHHHHH
AKVLMAEHVNYIASDIQPSVVTEGRKEGFPVYLGDLTKLEVLTAMGASRATAIAIAINNE
HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEECCHHHHHHHHHCCCCCCEEEEEEECCC
VTAKKVIALVAKNFPDTIIAVRIPDLSNADMYKKLGAHYLIPETYEAGLQLGGIALSISG
HHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCHHHCCCEECCEEEEEEC
FSDNAILSLQNAFRMEDYQRPVKKESSDDVR
CCCCCEEEHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA