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The map label for this gene is tilS [H]

Identifier: 88606751

GI number: 88606751

Start: 1251447

End: 1252790

Strand: Reverse

Name: tilS [H]

Synonym: APH_1180

Alternate gene names: 88606751

Gene position: 1252790-1251447 (Counterclockwise)

Preceding gene: 88607698

Following gene: 88607638

Centisome position: 85.15

GC content: 43.45

Gene sequence:

>1344_bases
TTGCGCGATATCGTTCCCAATGCACAGTATGCTGTTGCTGTCTCAGGTGGTGTAGATAGCATGACGCTTATGCGTCTTGT
CGCGCTATTTCATAAGAGTTCAGCATCTGTTGGTTCGCCGACTATTCTTACCGTAAACCATGGGTTTAGACCTGAAGCTA
GAAATGAAGTTAGCTTCGTGCACAAGCAAGCCACTATGCTAGGGCTGGAGTGTCACATGCTGCACTGGGAGAACCCTATT
CGAAAAAAGTCTCAAGTTGTTGCTAGAAATATACGGTACCAATTGTTGCTGCAATGGTGCTCTGCACATAACGTGAAGGT
TCTTCTTACAGCGCATACGAAAAATGATCAGGCAGAAACAGTATTAATCCGCTTAGAGAGAGGTAGTGGCATAGATGGGC
TTGCTGGTATAAGGGAGCGCTCTGTGATTGGGGATGTGACAATTATTAGACCTTTGCTAGATTTTACACGTCGGGAAATA
CAAGAATATGCTATTCAACATCAGCAACCATGGATTGAAGACCCTAGCAATAGTGACCCAAAATACAGACGAACTTTTTA
CCGGAACTTTATAAATAACTGCAAGCATCCGGAGATACTGATAAGCAGGCTAAGCAGCACAGCTCTGCATATGCAGCGGT
CCTTAAGCTGTATCTTACACTATGTACAGAATGCTTTGGACAGCTGTTTGGAATTTACTCCCTTCGGATACGTAGTAATC
AAGCACGATGTTTTTCGTAATTTGCAGGATGAGATCGCCAGCAGGTTGTTTCTGCTTCTCATCATGACTATTGGCAACAA
ACCTTTGAAGCCCAGGTTTTCTAAGTTCTCTTCTGCACTCTCTAAGGTACGTTCTACTGCTGGTTTTGCTTCTTTTACCT
TGCATGGCTGTATGATACTCAAGACGTGTGATAGTAACATTATTGTTGCTAGGGAAGTATCCAGTATAGTCCCTAAAACT
TATTTGCACGGTGAGACACGTCTACTGTGGGACTCTAGATTTGAGTTACATATTGAATTCTCAGATTGCTTTGACACGTC
TTTACCTTCTACCTCTGAGATGTCGGAAAAACAGGAATCCACGTGTGGTAGTGTAACAGAACGCAAGGCTGCTTCTGCGA
TGCAGGTATCCATCAGCCCGCTAGATTCTAATCCTATACCGGAGCATTTGCGGGGTATAAACAAATATGTAGTACAAGGG
TTGCCTGTGTTGATCTGCGGGGATAAAGTTCTTGCCTACCCGTTGCAAAAGCATAATATAAATGGTGTCCCTGTCGTTAG
CATAGAGAGGGTCTTGCTAAAGGAGAGGATGATGAACCTAATTTGTAATCAGCTAGATCAATAG

Upstream 100 bases:

>100_bases
GCCTGGAAAGTGTGTACACAGCAATGTGTCGGGGGTTCGAATCCCCCTCTTTCCGTATTTTGGGATGTCTGTGGGATTTG
AATCTAGCTCTTTCCGTAAG

Downstream 100 bases:

>100_bases
AGGTCGCTTATCGTGAACTTTAGGAGAGTGGTCGAGGGCTTAATTGTGTGGTCCGCAGTGATCGCACTTGCTGCTATGCT
ATATGCCCATTTTGGTGACA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MRDIVPNAQYAVAVSGGVDSMTLMRLVALFHKSSASVGSPTILTVNHGFRPEARNEVSFVHKQATMLGLECHMLHWENPI
RKKSQVVARNIRYQLLLQWCSAHNVKVLLTAHTKNDQAETVLIRLERGSGIDGLAGIRERSVIGDVTIIRPLLDFTRREI
QEYAIQHQQPWIEDPSNSDPKYRRTFYRNFINNCKHPEILISRLSSTALHMQRSLSCILHYVQNALDSCLEFTPFGYVVI
KHDVFRNLQDEIASRLFLLLIMTIGNKPLKPRFSKFSSALSKVRSTAGFASFTLHGCMILKTCDSNIIVAREVSSIVPKT
YLHGETRLLWDSRFELHIEFSDCFDTSLPSTSEMSEKQESTCGSVTERKAASAMQVSISPLDSNPIPEHLRGINKYVVQG
LPVLICGDKVLAYPLQKHNINGVPVVSIERVLLKERMMNLICNQLDQ

Sequences:

>Translated_447_residues
MRDIVPNAQYAVAVSGGVDSMTLMRLVALFHKSSASVGSPTILTVNHGFRPEARNEVSFVHKQATMLGLECHMLHWENPI
RKKSQVVARNIRYQLLLQWCSAHNVKVLLTAHTKNDQAETVLIRLERGSGIDGLAGIRERSVIGDVTIIRPLLDFTRREI
QEYAIQHQQPWIEDPSNSDPKYRRTFYRNFINNCKHPEILISRLSSTALHMQRSLSCILHYVQNALDSCLEFTPFGYVVI
KHDVFRNLQDEIASRLFLLLIMTIGNKPLKPRFSKFSSALSKVRSTAGFASFTLHGCMILKTCDSNIIVAREVSSIVPKT
YLHGETRLLWDSRFELHIEFSDCFDTSLPSTSEMSEKQESTCGSVTERKAASAMQVSISPLDSNPIPEHLRGINKYVVQG
LPVLICGDKVLAYPLQKHNINGVPVVSIERVLLKERMMNLICNQLDQ
>Mature_447_residues
MRDIVPNAQYAVAVSGGVDSMTLMRLVALFHKSSASVGSPTILTVNHGFRPEARNEVSFVHKQATMLGLECHMLHWENPI
RKKSQVVARNIRYQLLLQWCSAHNVKVLLTAHTKNDQAETVLIRLERGSGIDGLAGIRERSVIGDVTIIRPLLDFTRREI
QEYAIQHQQPWIEDPSNSDPKYRRTFYRNFINNCKHPEILISRLSSTALHMQRSLSCILHYVQNALDSCLEFTPFGYVVI
KHDVFRNLQDEIASRLFLLLIMTIGNKPLKPRFSKFSSALSKVRSTAGFASFTLHGCMILKTCDSNIIVAREVSSIVPKT
YLHGETRLLWDSRFELHIEFSDCFDTSLPSTSEMSEKQESTCGSVTERKAASAMQVSISPLDSNPIPEHLRGINKYVVQG
LPVLICGDKVLAYPLQKHNINGVPVVSIERVLLKERMMNLICNQLDQ

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=197, Percent_Identity=32.994923857868, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 50523; Mature: 50523

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIVPNAQYAVAVSGGVDSMTLMRLVALFHKSSASVGSPTILTVNHGFRPEARNEVSFV
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHH
HKQATMLGLECHMLHWENPIRKKSQVVARNIRYQLLLQWCSAHNVKVLLTAHTKNDQAET
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEE
VLIRLERGSGIDGLAGIRERSVIGDVTIIRPLLDFTRREIQEYAIQHQQPWIEDPSNSDP
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KYRRTFYRNFINNCKHPEILISRLSSTALHMQRSLSCILHYVQNALDSCLEFTPFGYVVI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
KHDVFRNLQDEIASRLFLLLIMTIGNKPLKPRFSKFSSALSKVRSTAGFASFTLHGCMIL
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCEEEE
KTCDSNIIVAREVSSIVPKTYLHGETRLLWDSRFELHIEFSDCFDTSLPSTSEMSEKQES
EECCCCEEEEEHHHHHCCHHHCCCCEEEEECCCEEEEEEEHHHCCCCCCCHHHHHHHHHH
TCGSVTERKAASAMQVSISPLDSNPIPEHLRGINKYVVQGLPVLICGDKVLAYPLQKHNI
HHCHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHCCCHHHCCC
NGVPVVSIERVLLKERMMNLICNQLDQ
CCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRDIVPNAQYAVAVSGGVDSMTLMRLVALFHKSSASVGSPTILTVNHGFRPEARNEVSFV
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHH
HKQATMLGLECHMLHWENPIRKKSQVVARNIRYQLLLQWCSAHNVKVLLTAHTKNDQAET
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEE
VLIRLERGSGIDGLAGIRERSVIGDVTIIRPLLDFTRREIQEYAIQHQQPWIEDPSNSDP
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KYRRTFYRNFINNCKHPEILISRLSSTALHMQRSLSCILHYVQNALDSCLEFTPFGYVVI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
KHDVFRNLQDEIASRLFLLLIMTIGNKPLKPRFSKFSSALSKVRSTAGFASFTLHGCMIL
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCEEEE
KTCDSNIIVAREVSSIVPKTYLHGETRLLWDSRFELHIEFSDCFDTSLPSTSEMSEKQES
EECCCCEEEEEHHHHHCCHHHCCCCEEEEECCCEEEEEEEHHHCCCCCCCHHHHHHHHHH
TCGSVTERKAASAMQVSISPLDSNPIPEHLRGINKYVVQGLPVLICGDKVLAYPLQKHNI
HHCHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHCCCHHHCCC
NGVPVVSIERVLLKERMMNLICNQLDQ
CCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA