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The map label for this gene is dnaG [H]

Identifier: 88606745

GI number: 88606745

Start: 597914

End: 599737

Strand: Direct

Name: dnaG [H]

Synonym: APH_0571

Alternate gene names: 88606745

Gene position: 597914-599737 (Clockwise)

Preceding gene: 88607494

Following gene: 88607646

Centisome position: 40.64

GC content: 40.02

Gene sequence:

>1824_bases
ATGCAGAATAATAATTCTATCACCGATAATATAACCTTACTGAGAAGTAAAATAAGGCTCATTGATGTAGTAGCAGGGAA
GATTAAGTTAACCAAGAGGAGTGGGAACAACTATGTGGGTTTGTGTCCATTTCATTCAGAAAAAACCCCTTCATTCAATA
TTAATAGTGAAAATGAGTTATTCTACTGTTTTGGATGTGGGGCAACAGGTGATGTAATACAGTTTGTATCTGATACGGAA
GGGCTGGATTTTAAGCAGGCCATGCAATACCTTTCTGAGCGGTACCACGTAGAGCTTCTAGATGATAAGAGGCCTGATTC
GTCACATAGCGCTCTATATGATGTTATGGAAAAAGCGGCGGTATTCCTTTCGCAGCAACTAAGTGTTTCAAAAGCTGCCT
TATCGTATCTCAAAGATCGGGGAATCTCGGATAGTACCATCAATAGATTCAGGTTAGGATATGTGACTTCCACTGGGATA
AAAGCGCACCTTTTGTCTTTGGGCATTAGCATGGAAAAAATGAGCGAGACTGGTTTGCTGACTAAAAGCGTCCAGGATTG
TCTTTATAATAGGATCATATTTCCAATATGTAACCCAATGGGGAAGGTTATTGCCCTTGGGGGCAGGTCTATTACTCCAG
GACATACTCCAAAGTATCTAAACTCCGCAGAAAATGCATTATTCAAAAAAAGAGAGTGCATGTATGGCTTACATCTAGCT
ATGGGGCAAGCAAAGAAGCTAGGTAAGATCATAGTTGTGGAAGGCTACATGGATGTCTTAATGCTCAGTCAGTTGGGTAT
CCACAATGTGGTGGGATTGTTGGGAACGTCAATGACGGAGTTTCATCTGAAAAACATTTGGAGTGTTGTACCTGAGATAA
TAGTATGGCTTGATGGAGATTTTGCAGGCTTAAAAGCATCGGTAAAAATTGCTCAACTTGCAGTATCTATCATGAAGTCT
GGTTCCAGCATTAGGTTTATATCTATTGCAGAAGGTAAGGATCCATATGATATATGCAGAGAACAAAGCATTGACTTTGT
GAAAGACCTAATAGAACGCGCTAAGTTGCTTTCAGAATTTATATGGGAGTATGAACTCTCTAGCAGTGGTATAGGCGATA
AAGTCGTGCCAGAGCAGTGCATGATGCTTGAAGGAAAGATAAAAGAATATACTTCAAGAATACAAGATCCCAATGTAGCA
AAGCACTATAGGAATTTCTTTTATCAGCAGATGAAGGCACTGCAAGGGTATAAAAAGTGGAACGCGAATTCGTATGCAGG
AACTGCGGTTAGTAGAGCAAATAAGGTGAAATCGTGTGACAGACTTCAAGATGTATCAATGAAAGTATGTATGGAAGAGA
ACTATCAGCTGCGTGTTATGTACACAATTATAGAGTGTCCTAAACTTTTAGATGATCCTGCGATTTTTGAACAGTTTGCT
AGTATAGATTTTTCCTCTGTAGACAGAAGGGTGCTACAGCAACATATTGTTGATATAAAAAGTACCTGTGACAACGTGTT
TTTGAAGCAGGATCTTGTGGAAAAGCTTAGGCCACGAGGGCAGAATCTTGAGGCGATTCTTCAATACATAATAAGAAGTA
TGGCAAATGTTGGATGTGCGTTTATAGTGCCGGGACTAAGCGACGAGAGTATAGAAAAATTGGCTAAGCAGGAATGGGAA
AAGCTTATGTTATCTAGGCAGCTGAACGAGATTCACGAGCAGATAGTAAAGCTAAGACTCGAAGGAAAGAATGATGTAGC
CTTGAACTTATCTGAGTATGCTCGCGAGATAGATGATAAATTGCGTAGCCTGTGGAAATGCTGA

Upstream 100 bases:

>100_bases
TAACGTCTGTTGCGAACAGCAAGTATTATGTGAGTAGTCGACTGGTGTGACAACAAGATGCACTGGGAGTATAATCTTCA
GTGTGATGTTGGGGAGTATC

Downstream 100 bases:

>100_bases
AACCTGTTTCAAGTGGCAATCAGCATAATACTTCCGAATTTTATGCTTCAAGGCATTGCTTGAGATTCATGTACCTAAGA
TCCTACTATTGATTGTATTC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MQNNNSITDNITLLRSKIRLIDVVAGKIKLTKRSGNNYVGLCPFHSEKTPSFNINSENELFYCFGCGATGDVIQFVSDTE
GLDFKQAMQYLSERYHVELLDDKRPDSSHSALYDVMEKAAVFLSQQLSVSKAALSYLKDRGISDSTINRFRLGYVTSTGI
KAHLLSLGISMEKMSETGLLTKSVQDCLYNRIIFPICNPMGKVIALGGRSITPGHTPKYLNSAENALFKKRECMYGLHLA
MGQAKKLGKIIVVEGYMDVLMLSQLGIHNVVGLLGTSMTEFHLKNIWSVVPEIIVWLDGDFAGLKASVKIAQLAVSIMKS
GSSIRFISIAEGKDPYDICREQSIDFVKDLIERAKLLSEFIWEYELSSSGIGDKVVPEQCMMLEGKIKEYTSRIQDPNVA
KHYRNFFYQQMKALQGYKKWNANSYAGTAVSRANKVKSCDRLQDVSMKVCMEENYQLRVMYTIIECPKLLDDPAIFEQFA
SIDFSSVDRRVLQQHIVDIKSTCDNVFLKQDLVEKLRPRGQNLEAILQYIIRSMANVGCAFIVPGLSDESIEKLAKQEWE
KLMLSRQLNEIHEQIVKLRLEGKNDVALNLSEYAREIDDKLRSLWKC

Sequences:

>Translated_607_residues
MQNNNSITDNITLLRSKIRLIDVVAGKIKLTKRSGNNYVGLCPFHSEKTPSFNINSENELFYCFGCGATGDVIQFVSDTE
GLDFKQAMQYLSERYHVELLDDKRPDSSHSALYDVMEKAAVFLSQQLSVSKAALSYLKDRGISDSTINRFRLGYVTSTGI
KAHLLSLGISMEKMSETGLLTKSVQDCLYNRIIFPICNPMGKVIALGGRSITPGHTPKYLNSAENALFKKRECMYGLHLA
MGQAKKLGKIIVVEGYMDVLMLSQLGIHNVVGLLGTSMTEFHLKNIWSVVPEIIVWLDGDFAGLKASVKIAQLAVSIMKS
GSSIRFISIAEGKDPYDICREQSIDFVKDLIERAKLLSEFIWEYELSSSGIGDKVVPEQCMMLEGKIKEYTSRIQDPNVA
KHYRNFFYQQMKALQGYKKWNANSYAGTAVSRANKVKSCDRLQDVSMKVCMEENYQLRVMYTIIECPKLLDDPAIFEQFA
SIDFSSVDRRVLQQHIVDIKSTCDNVFLKQDLVEKLRPRGQNLEAILQYIIRSMANVGCAFIVPGLSDESIEKLAKQEWE
KLMLSRQLNEIHEQIVKLRLEGKNDVALNLSEYAREIDDKLRSLWKC
>Mature_607_residues
MQNNNSITDNITLLRSKIRLIDVVAGKIKLTKRSGNNYVGLCPFHSEKTPSFNINSENELFYCFGCGATGDVIQFVSDTE
GLDFKQAMQYLSERYHVELLDDKRPDSSHSALYDVMEKAAVFLSQQLSVSKAALSYLKDRGISDSTINRFRLGYVTSTGI
KAHLLSLGISMEKMSETGLLTKSVQDCLYNRIIFPICNPMGKVIALGGRSITPGHTPKYLNSAENALFKKRECMYGLHLA
MGQAKKLGKIIVVEGYMDVLMLSQLGIHNVVGLLGTSMTEFHLKNIWSVVPEIIVWLDGDFAGLKASVKIAQLAVSIMKS
GSSIRFISIAEGKDPYDICREQSIDFVKDLIERAKLLSEFIWEYELSSSGIGDKVVPEQCMMLEGKIKEYTSRIQDPNVA
KHYRNFFYQQMKALQGYKKWNANSYAGTAVSRANKVKSCDRLQDVSMKVCMEENYQLRVMYTIIECPKLLDDPAIFEQFA
SIDFSSVDRRVLQQHIVDIKSTCDNVFLKQDLVEKLRPRGQNLEAILQYIIRSMANVGCAFIVPGLSDESIEKLAKQEWE
KLMLSRQLNEIHEQIVKLRLEGKNDVALNLSEYAREIDDKLRSLWKC

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=367, Percent_Identity=33.5149863760218, Blast_Score=216, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68642; Mature: 68642

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNNNSITDNITLLRSKIRLIDVVAGKIKLTKRSGNNYVGLCPFHSEKTPSFNINSENEL
CCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCE
FYCFGCGATGDVIQFVSDTEGLDFKQAMQYLSERYHVELLDDKRPDSSHSALYDVMEKAA
EEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
VFLSQQLSVSKAALSYLKDRGISDSTINRFRLGYVTSTGIKAHLLSLGISMEKMSETGLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCHHHHHHHHCCCHHHHHHCCHH
TKSVQDCLYNRIIFPICNPMGKVIALGGRSITPGHTPKYLNSAENALFKKRECMYGLHLA
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGQAKKLGKIIVVEGYMDVLMLSQLGIHNVVGLLGTSMTEFHLKNIWSVVPEIIVWLDGD
HHHHHHCCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCC
FAGLKASVKIAQLAVSIMKSGSSIRFISIAEGKDPYDICREQSIDFVKDLIERAKLLSEF
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
IWEYELSSSGIGDKVVPEQCMMLEGKIKEYTSRIQDPNVAKHYRNFFYQQMKALQGYKKW
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
NANSYAGTAVSRANKVKSCDRLQDVSMKVCMEENYQLRVMYTIIECPKLLDDPAIFEQFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCHHHCCCHHHHHHHH
SIDFSSVDRRVLQQHIVDIKSTCDNVFLKQDLVEKLRPRGQNLEAILQYIIRSMANVGCA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEE
FIVPGLSDESIEKLAKQEWEKLMLSRQLNEIHEQIVKLRLEGKNDVALNLSEYAREIDDK
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
LRSLWKC
HHHHHCC
>Mature Secondary Structure
MQNNNSITDNITLLRSKIRLIDVVAGKIKLTKRSGNNYVGLCPFHSEKTPSFNINSENEL
CCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCE
FYCFGCGATGDVIQFVSDTEGLDFKQAMQYLSERYHVELLDDKRPDSSHSALYDVMEKAA
EEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
VFLSQQLSVSKAALSYLKDRGISDSTINRFRLGYVTSTGIKAHLLSLGISMEKMSETGLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCHHHHHHHHCCCHHHHHHCCHH
TKSVQDCLYNRIIFPICNPMGKVIALGGRSITPGHTPKYLNSAENALFKKRECMYGLHLA
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGQAKKLGKIIVVEGYMDVLMLSQLGIHNVVGLLGTSMTEFHLKNIWSVVPEIIVWLDGD
HHHHHHCCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCC
FAGLKASVKIAQLAVSIMKSGSSIRFISIAEGKDPYDICREQSIDFVKDLIERAKLLSEF
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
IWEYELSSSGIGDKVVPEQCMMLEGKIKEYTSRIQDPNVAKHYRNFFYQQMKALQGYKKW
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
NANSYAGTAVSRANKVKSCDRLQDVSMKVCMEENYQLRVMYTIIECPKLLDDPAIFEQFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCHHHCCCHHHHHHHH
SIDFSSVDRRVLQQHIVDIKSTCDNVFLKQDLVEKLRPRGQNLEAILQYIIRSMANVGCA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEE
FIVPGLSDESIEKLAKQEWEKLMLSRQLNEIHEQIVKLRLEGKNDVALNLSEYAREIDDK
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
LRSLWKC
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8422995; 9823893 [H]