Click here to switch to the map view.

The map label for this gene is cysJ [H]

Identifier: 88196572

GI number: 88196572

Start: 2710641

End: 2712521

Strand: Reverse

Name: cysJ [H]

Synonym: SAOUHSC_02947

Alternate gene names: 88196572

Gene position: 2712521-2710641 (Counterclockwise)

Preceding gene: 88196574

Following gene: 88196571

Centisome position: 96.14

GC content: 34.88

Gene sequence:

>1881_bases
TTGAAATTAAATACATCTAATAGTCCATTTACAGAAAAACAAGTCACAGAGATTAACAATCTGCTGCAAACATTAACAGA
GAGCCAACAACAGTGGCTTAGTGGTTTTTTACTAGCTAATAGTAACGATACGACAAGTGATAGTAATCAACAACAATTAG
AGACAGAAGTGTGGCAACAATCACAAATATCAGAAGAACAAGCAACTTCAACAACGTATATGTTACAAAATAAAGAGCCA
CATATCGAAGCTAATCAGCGGCATGTTACAGTGCTATATGGTTCTGAATCAGGTAATGCCATGCGTTTAGCTGAAATTTT
TTCAGAACGTTTAAGTGATATCGGACATCAAGTTGTTTTGATGTCAATGGATGAATATGATACGACAAACATCGCGCAGT
TAGAAGATTTATTTATTATTACGTCTACTCATGGTGAAGGAGAACCGCCTGATAATGCATGGGATTTCTTTGAATTTTTA
GAAGACGATAACGCACCTAATTTAAATCATGTGAGATATTCAGTACTAGCTTTAGGTGATCAAACATATGAATTTTTCTG
TCAAGCCGGTAAAGATGTAGATGTTTTACTAGAAAATCTAGGCGCTGAGCGTATATGTAAGCGTGTAGATTGTGATATTG
ATTATGAAGAAGACGCAGAAAAGTGGATGGCAGACATCATTAATATTATTGATACCACATCAGAAGGTATTCAAAGTGAA
TCGGTGATAAGTGAATCAATTAAGTCTGCCAAAGAAAAGAAATATTCTAAATCAAATCCATACCAAGCAGAAGTATTAGC
GAATATCAATTTAAATGGTACCGATTCAAATAAAGAAACACGACATATAGAATTTTTACTTGATGATTTTAGTGAATCAT
ATGAACCAGGAGATTGTATAGTAGCATTACCGCAAAACGACCCTGAATTGGTTGAAAAACTAATATCCATGTTAGGTTGG
GATCCGCAATCTCCGGTGCCAATTAATGATCATGGTGATACAGTTCCTATTGTTGAAGCACTAACATCACATTTTGAATT
TACTAAATTAACATTGCCATTATTGAAAAATGCAGATATCTATTTTGACAATGAAGAATTATCTGAACGTATTCAAGATG
AGTCATGGGCGCGTGAATATGTTATAAATCGGGACTTTATAGATTTAATAACAGATTTTCCAACTATAGAATTACAACCT
GAGAATATGTATCAAATCCTTAGAAAATTACCACCAAGAGAGTATTCGATTTCTAGTAGTTTTATGGCAACGCCAGATGA
AGTGCATATTACCGTTGGTACGGTTCGTTATCAAGCACATGGACGTGAGAGAAAAGGTGTATGCTCGGTTCATTTTGCTG
AGCGAATTAAACCAGGCGATATAGTACCAATTTATTTGAAGAAAAATCCGAACTTCAAATTTCCGATGAAGCAAGATATA
CCGGTTATTATGATTGGACCAGGTACTGGAATTGCTCCTTTTAGAGCATATTTACAAGAACGTGAAGAACTTGGTATGAC
TGGAAAAACATGGTTGTTCTTTGGTGATCAACACCGTAGTTCTGACTTTTTATATGAAGAAGAAATAGAAGAATGGCTTG
AAAATGGAAACTTAACACGCGTAGATTTAGCATTTTCAAGAGACCAAGAACACAAAGAATATGTACAGCATCGTATAATG
GAAGAAAGTAAACGTTTCAATGAATGGATTGAGCAAGGCGCAGCAATCTATATTTGTGGCGATGAAAAATGTATGGCGAA
AGATGTCCATCAAGCCATTAAAGATGTATTGGTAAAAGAACGTCATATTTCTCAAGAAGAAGCAGAGTTATTATTGCGAC
AAATGAAACAACAACAACGCTATCAACGTGATGTTTATTAG

Upstream 100 bases:

>100_bases
AAGTTTGTTTTAATTTATACCATACATATTAAAAAATTGACTAAACTATCGATAAAAAAAGAGTCAATCATCTATTTCAA
GTTTATTGAGGTGAAAACAT

Downstream 100 bases:

>100_bases
CGATTGGTGTTAAATATTTTAAGGTGTAATGATGTAAAAAGATATAAAGGATGTTGCTCAACATGAATATGCCATTAATG
ATAGATTTAACAAATAAAAA

Product: sulfite reductase (NADPH) flavoprotein alpha-component

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
ENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESKRFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY

Sequences:

>Translated_626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
ENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESKRFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY
>Mature_626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
ENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESKRFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=612, Percent_Identity=30.718954248366, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI7657393, Length=597, Percent_Identity=28.3082077051926, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI10835173, Length=655, Percent_Identity=28.0916030534351, Blast_Score=209, Evalue=6e-54,
Organism=Homo sapiens, GI221316705, Length=606, Percent_Identity=27.8877887788779, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI221316709, Length=597, Percent_Identity=28.1407035175879, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI40254422, Length=664, Percent_Identity=27.5602409638554, Blast_Score=196, Evalue=6e-50,
Organism=Homo sapiens, GI221316707, Length=520, Percent_Identity=28.8461538461538, Blast_Score=196, Evalue=8e-50,
Organism=Homo sapiens, GI24041029, Length=599, Percent_Identity=27.0450751252087, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI169790956, Length=425, Percent_Identity=28.4705882352941, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI169790958, Length=425, Percent_Identity=28.4705882352941, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1789123, Length=539, Percent_Identity=39.7031539888683, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1790181, Length=152, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789154, Length=126, Percent_Identity=31.7460317460317, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554134, Length=605, Percent_Identity=30.0826446280992, Blast_Score=251, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17566446, Length=572, Percent_Identity=26.5734265734266, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17531441, Length=255, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321832, Length=647, Percent_Identity=26.1205564142195, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325305, Length=633, Percent_Identity=25.1184834123223, Blast_Score=156, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6321143, Length=390, Percent_Identity=27.6923076923077, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17137192, Length=612, Percent_Identity=30.0653594771242, Blast_Score=245, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24582192, Length=514, Percent_Identity=29.7665369649805, Blast_Score=203, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24583543, Length=533, Percent_Identity=29.4559099437148, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI78706876, Length=533, Percent_Identity=29.4559099437148, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI78706872, Length=533, Percent_Identity=29.4559099437148, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24660907, Length=599, Percent_Identity=25.542570951586, Blast_Score=173, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24660903, Length=599, Percent_Identity=25.542570951586, Blast_Score=173, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24647438, Length=414, Percent_Identity=25.3623188405797, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 72213; Mature: 72213

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHH
SQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVL
HHCCHHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEE
MSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFLEDDNAPNLNHVRYSVLALGD
EECCCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHEEEEEEECC
QTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
HHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCI
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEE
VALPQNDPELVEKLISMLGWDPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADI
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHEEHHHHCCCCE
YFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQPENMYQILRKLPPREYSISSS
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCHHHHHHHHHCCCCCEECCCC
FMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
CCCCCCEEEEEEEEEEEEECCCCCCCEEEEEHHHCCCCCCEEEEEEECCCCCCCCCCCCC
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTR
CEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEE
VDLAFSRDQEHKEYVQHRIMEESKRFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RHISQEEAELLLRQMKQQQRYQRDVY
HHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHH
SQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVL
HHCCHHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEE
MSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFLEDDNAPNLNHVRYSVLALGD
EECCCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHEEEEEEECC
QTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
HHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCI
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEE
VALPQNDPELVEKLISMLGWDPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADI
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHEEHHHHCCCCE
YFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQPENMYQILRKLPPREYSISSS
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCHHHHHHHHHCCCCCEECCCC
FMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
CCCCCCEEEEEEEEEEEEECCCCCCCEEEEEHHHCCCCCCEEEEEEECCCCCCCCCCCCC
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTR
CEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEE
VDLAFSRDQEHKEYVQHRIMEESKRFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RHISQEEAELLLRQMKQQQRYQRDVY
HHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]