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The map label for this gene is nikA [H]

Identifier: 88196403

GI number: 88196403

Start: 2542857

End: 2544455

Strand: Reverse

Name: nikA [H]

Synonym: SAOUHSC_02767

Alternate gene names: 88196403

Gene position: 2544455-2542857 (Counterclockwise)

Preceding gene: 88196404

Following gene: 88196402

Centisome position: 90.19

GC content: 34.77

Gene sequence:

>1599_bases
ATGAGAAAACTAACTAAAATGAGTGCAATGTTACTTGCATCAGGGCTAATTTTAACTGGTTGTGGCGGTAATAAAGGTTT
AGAGGAGAAAAAAGAAAACAAGCAATTAACGTATACGACGGTTAAAGATATCGGTGATATGAATCCGCATGTTTACGGTG
GATCAATGTCTGCTGAAAGTATGATATACGAGCCGCTTGTACGTAACACGAAAGATGGTATTAAGCCTTTACTAGCTAAA
AAGTGGGATGTGTCTGAAGATGGGAAGACATACACGTTCCATTTGAGAGATGACGTTAAATTCCATGATGGTACGCCATT
TGATGCTGACGCAGTTAAGAAAAATATTGACGCAGTTCAAGAAAACAAAAAATTGCATTCTTGGTTAAAGATTTCGACAT
TAATTGACAATGTTAAAGTTAAAGATAAGTACACGGTTGAATTGAATTTGAAAGAAGCATATCAACCTGCATTGGCTGAA
TTAGCGATGCCTCGTCCATATGTATTTGTGTCTCCAAAAGACTTTAAAAACGGTACAACAAAAGATGGCGTTAAAAAGTT
CGATGGTACTGGTCCATTTAAATTAGGTGAACACAAAAAAGATGAGTCTGCAGACTTTAACAAAAATGATCAATACTGGG
GCGAAAAGTCTAAACTTAACAAAGTACAAGCAAAAGTAATGCCTGCTGGTGAAACAGCATTCCTATCAATGAAAAAAGGT
GAAACGAACTTTGCCTTCACAGATGATAGAGGTACAGATAGCTTAGACAAAGACTCTTTAAAACAATTGAAAGATACAGG
TGACTATCAAGTTAAGCGTAGTCAACCTATGAATACGAAAATGTTAGTTGTCAATTCTGGTAAAAAAGATAACGCTGTGA
GTGACAAAACAGTCAGACAAGCGATTGGTCATATGGTAAACAGAGATAAAATTGCCAAAGAAATTTTAGATGGTCAAGAA
AAACCAGCAACTCAATTATTTGCGAAAAATGTAACAGACATTAATTTCGATATGCCAACACGTAAGTATGACCTTAAAAA
AGCAGAATCATTATTAGATGAAGCTGGTTGGAAGAAAGGTAAAGACAGCGATGTTCGTCAAAAAGATGGTAAAAACCTTG
AAATGGCAATGTACTATGACAAAGGTTCTTCAAGTCAAAAAGAACAAGCAGAATACTTACAAGCAGAATTTAAGAAAATG
GGTATTAAGTTAAACATCAATGGCGAAACATCAGATAAAATTGCTGAACGTCGTACTTCTGGTGATTATGACTTAATGTT
CAACCAAACTTGGGGATTATTGTACGATCCACAAAGTACTATTGCAGCATTTAAAGAGAAAAATGGTTATGAAAGTGCAA
CATCAGGCATTGAGAACAAAGATAAAATATACAACAGCATTGATGACGCATTTAAAATCCAAAACGGTAAAGAGCGTTCA
GACGCTTATAAAAACATTTTGAAACAAATTGATGATGAAGGTATCTTTATCCCTATTTCACACGGTAGTATGACAGTTGT
TGCACCAAAAGATTTAGAAAAAGTATCATTCACACAATCACAGTATGAATTACCATTCAATGAAATGCAGTATAAATAA

Upstream 100 bases:

>100_bases
TGTTTGACATTTACATAAAAATAAGCAAATAATTGAGAAAAATAATCATTACGATTTGATTAAGTAATGCAACTTATCAA
TTTAGAAAGAGGAAAAGCAA

Downstream 100 bases:

>100_bases
AGGAGCAATTAGATGTTCAAATTTATCTTAAAACGTATTGCGCTCATGTTTCCATTGATGATTGTAGTAAGTTTTATGAC
ATTTCTATTGACGTATATTA

Product: peptide ABC transporter peptide-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAK
KWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQE
KPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKM
GIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKEKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

Sequences:

>Translated_532_residues
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAK
KWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQE
KPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKM
GIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKEKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK
>Mature_532_residues
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAK
KWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQE
KPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKM
GIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKEKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=499, Percent_Identity=34.2685370741483, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1789966, Length=538, Percent_Identity=27.5092936802974, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1787052, Length=339, Percent_Identity=25.3687315634218, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787762, Length=456, Percent_Identity=22.8070175438596, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081878, Length=390, Percent_Identity=22.5641025641026, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787551, Length=281, Percent_Identity=23.1316725978648, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60078; Mature: 60078

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAES
CCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCEEEEEEHHHHCCCCCEEECCCCCHHH
MIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQ
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHH
ENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTT
HHHHHHHHHHHHHHHHCEEECCEEEEEEEHHHHHHHHHHHHCCCCCEEEECCHHCCCCCC
KDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEEEEEECC
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQ
CCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHH
AIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
KDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTS
CCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCC
GDYDLMFNQTWGLLYDPQSTIAAFKEKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
CCEEEEEECCCCEEECCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHH
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK
HHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHCCHHHCCCHHHHCCC
>Mature Secondary Structure
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAES
CCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCEEEEEEHHHHCCCCCEEECCCCCHHH
MIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQ
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHH
ENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTT
HHHHHHHHHHHHHHHHCEEECCEEEEEEEHHHHHHHHHHHHCCCCCEEEECCHHCCCCCC
KDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEEEEEECC
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQ
CCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHH
AIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
KDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTS
CCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCC
GDYDLMFNQTWGLLYDPQSTIAAFKEKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
CCEEEEEECCCCEEECCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHH
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK
HHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHCCHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]