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The map label for this gene is narH [H]

Identifier: 88196320

GI number: 88196320

Start: 2462342

End: 2463787

Strand: Reverse

Name: narH [H]

Synonym: SAOUHSC_02680

Alternate gene names: 88196320

Gene position: 2463787-2462342 (Counterclockwise)

Preceding gene: 88196321

Following gene: 88196319

Centisome position: 87.33

GC content: 38.04

Gene sequence:

>1446_bases
ATGTGGTTCAACAACGTAGAAACGAAGCCAGGTGTAGGGTATCCGAAACGTTGGGAAGACCAAGAACACTACAAAGGTGG
TTGGGTACTAAATCGTAAAGGGAAACTTGAATTAAAATCTGGAAGTAGAATTTCAAAAATTGCTTTAGGTAAAATTTTTT
ATAACCCAGATATGCCATTAATTAAAGATTATTATGAGCCATGGAACTATAATTATGAACATTTAACAACTGCGAAATCA
GGGAAGCATTCGCCAGTTGCTAGAGCGTATTCAGAAATTACGGGGGATAACATTGAAATTGAATGGGGACCTAACTGGGA
AGATGACTTAGCAGGTGGTCATGTTACAGGTCCAAAAGATCCTAACATCCAAAAAATAGAAGAAGATATTAAATTCCAAT
TTGACGAAACTTTTATGATGTATTTACCACGTTTATGTGAACATTGCTTGAATCCAAGTTGCGTTGCATCGTGTCCTTCA
GGTGCTATGTATAAACGTGATGAAGATGGCATTGTATTAGTTGACCAAGACGCATGTCGTGGATGGCGTTACTGTATGAC
AGGTTGTCCATATAAGAAAGTTTACTTCAATTGGAAAACGAACAAAGCTGAAAAGTGTACTTTCTGTTTCCCGAGAATCG
AGGCGGGTATGCCTACGGTATGCTCTGAAACTTGTACAGGACGTATGCGTTACTTAGGTGTTTTATTATATGATGCAGAT
CGAGTTCATGAAGCAGCTTCAGCAGTAGACGAGAAAGATTTATATGAAAAACAATTAGATATATTCTTAAACCCATTTGA
CGAAGAAGTCATTGCTCAAGCTGAAAAAGATGGTATTGGTTACGATTGGATTGAAGCTGCACAAAACTCTCCAATTTATA
AGTTAGCAATTGAATATAAACTTGCATTTCCGTTACATCCTGAATTTAGAACGATGCCGATGGTTTGGTATTGCCCACCA
CTTAGCCCAATTATGAGCTATTTTGAAGGTAAAAATACAACTCAAAATCCAGATGCGATTTTCCCAGCCATTGAAGAAAT
GCGTTTACCTATTGAATACTTAGCTAATATTTTCACTGCAGGAGATACAGAGCCAGTAAAAGGAGCCCTACAACGTATGG
CAATGATGAGAAGTTATATGAGATCTCAAGTTACTCAACAGCCATTTGACACGTCTCGATTAGAGCGATTAGGTATAACC
GAGCGCCAAACTAAAGACATGTATCGTTTATTAGGACTAGCCAAATATGAAGATCGATTTGTTATTCCAACATCACACAA
AGAAACCTATTTAGATACGTATCACGCACAAGGTAGTACAGGATACAATTACGGCGGCGAGCATTTTGGAGATAACTGTG
AAGGCTGTGGCGTTGCAGTAGGTTCAGGGAAAACTGGTCAAGAAATTTATAATGAGAATTTCTATGGAGGGATTTTCCGT
GATTAA

Upstream 100 bases:

>100_bases
GCAAGTTGCGATGGTATTAAATTTAGATAAATGCATAGGATGCCATACGTGTAGTGTGACATGTAAAAACACTTGGACAA
ATCGTCCAGGTGCTGAGTAC

Downstream 100 bases:

>100_bases
TTTCGATAATTTTAAAAAATATCAAGAAAGTTTTGGATATATGGCACAACAGTTATGTTTTCCAGAAAAATTAACTTTTC
ATCCAAAAACTTTTGAAGAA

Product: nitrate reductase, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKS
GKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPS
GAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDAD
RVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPP
LSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGIT
ERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFR
D

Sequences:

>Translated_481_residues
MWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKS
GKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPS
GAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDAD
RVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPP
LSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGIT
ERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFR
D
>Mature_481_residues
MWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKS
GKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPS
GAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDAD
RVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPP
LSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGIT
ERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFR
D

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=456, Percent_Identity=58.5526315789474, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1787740, Length=455, Percent_Identity=56.9230769230769, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1787872, Length=109, Percent_Identity=40.3669724770642, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787122, Length=109, Percent_Identity=40.3669724770642, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510944, Length=92, Percent_Identity=40.2173913043478, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=35.632183908046, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 55220; Mature: 55220

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPL
CCCCCCCCCCCCCCCCCCCCHHHCCCCEEECCCCCEEECCCCCCHHEEEEEEEECCCCCH
IKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKD
HHHHCCCCCCCHHEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCCC
PNIQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
CCHHHHHHHHHEECCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHC
GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDAD
CHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHCCCCCHHHHHHHCCHHHHHEEEEECHH
RVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYK
HHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEE
LAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTA
EECCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCC
GDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEE
VIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFR
EEECCCCCHHHHHHCCCCCCCCCCCCHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCC
D
C
>Mature Secondary Structure
MWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPL
CCCCCCCCCCCCCCCCCCCCHHHCCCCEEECCCCCEEECCCCCCHHEEEEEEEECCCCCH
IKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKD
HHHHCCCCCCCHHEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCCC
PNIQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
CCHHHHHHHHHEECCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHC
GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDAD
CHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHCCCCCHHHHHHHCCHHHHHEEEEECHH
RVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYK
HHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEE
LAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTA
EECCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCC
GDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEE
VIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFR
EEECCCCCHHHHHHCCCCCCCCCCCCHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]