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The map label for this gene is rihB [H]

Identifier: 88196198

GI number: 88196198

Start: 2347253

End: 2348194

Strand: Reverse

Name: rihB [H]

Synonym: SAOUHSC_02553

Alternate gene names: 88196198

Gene position: 2348194-2347253 (Counterclockwise)

Preceding gene: 88196199

Following gene: 88196197

Centisome position: 83.23

GC content: 34.08

Gene sequence:

>942_bases
GTGACTAAGAAAGTTTATTTTAACCACGATGGTGGTGTAGATGATTTAGTATCTCTATTTTTATTATTACAAATGGAAAA
CGTTCAATTGATAGGGGTCAGTACAATTGGTGCTGATTGTTATTTAGAGCCATCTTTGAGCGCATCAGTAAAAATTATTA
ATCGTTTTTCAAATGAAGATATTCAAGTTGCGCCATCATATGAACGAGGAAAAAATCCATTTCCTAAAGAATGGCGTATG
CATGCCTTTTTTATGGACGCATTGCCAATTTTAAATGAGCCAGTCAAACATGTTGCTTCAAATGTGAGCGACAAAGAAGC
CTTTGAAGACATTATTCAAACTTTAAAGAGACAATCAGAAAAAGTAACATTATTATTTACAGGCCCGCTTACAGATTTAG
CAAAAGCACTACAAAAAGATTCATCTATCGTTCAGTATATAGAAAAATTAGTTTGGATGGGTGGCACCTTTTTACCAAAA
GGAAATGTTGAAGAACCTGAGCATGATGGTTCTGCAGAATGGAATGCATATTGGGATCCAGAAGCGGTTAAAATTGTTTT
TGATAGCGATATAGAGATTGATATGGTTGCTTTAGAAAGTACGAATCAAGTACCGCTAACGTTAGATGTTAGACAAAGAT
GGGCGAATGAACGCCAATATACGGGCATTGATTTTTTAGGAGTAAGTTATGCAGCGGTACCACCATTAACACACTTTATA
ACAAATTCTACTTACTTTTTATGGGATGTTTTAACGACTGCTTATATTGGTAACAAGGACTTGGTTCATTCAATTGAGAA
AAAAGTCGATGTAATAAGTTATGGACCAAGTCAAGGTAAGACATTTGAGTGTAAAGATGGGCGCAAAATTAATGTCATAA
ATCATGTAGATAACAACGCATTTTTTGATTATATAACTGCACTTGCTAAAAAAGTAAATTAA

Upstream 100 bases:

>100_bases
GATTACAACAGAATGTTATGATTTTGTAGAAAGATATATAACAACGTTTTATAAAAACATAATATTGTTAATGGAAAATG
AAATGTAAGGGGGATTTCGA

Downstream 100 bases:

>100_bases
CAGCTGTGTAGAATAATTAAGGTTTTAATTTATATAGAACAACTTATTGTAAACTTTTCATTTCTTAAAGTTTACAATGG
TGCTATAATAATGGTCATGA

Product: hypothetical protein

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEPSLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRM
HAFFMDALPILNEPVKHVASNVSDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANERQYTGIDFLGVSYAAVPPLTHFI
TNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGPSQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN

Sequences:

>Translated_313_residues
MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEPSLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRM
HAFFMDALPILNEPVKHVASNVSDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANERQYTGIDFLGVSYAAVPPLTHFI
TNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGPSQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN
>Mature_312_residues
TKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEPSLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMH
AFFMDALPILNEPVKHVASNVSDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPKG
NVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANERQYTGIDFLGVSYAAVPPLTHFIT
NSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGPSQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=281, Percent_Identity=25.2669039145907, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786213, Length=103, Percent_Identity=43.6893203883495, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786871, Length=218, Percent_Identity=26.605504587156, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI32565474, Length=193, Percent_Identity=26.9430051813472, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 35362; Mature: 35231

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEPSLSASVKIINRFSNED
CCCEEEECCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCEEECCCCCHHHHHHHHCCCCC
IQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNVSDKEAFEDIIQTLKRQSE
EEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
KVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPKGNVEEPEHDGSAEWNAYWDP
EEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECC
EAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANERQYTGIDFLGVSYAAVPPLTHFI
CEEEEEECCCCEEEEEEECCCCCCEEEEEHHHHHCCCCCCCCEEEECCCHHHHCHHHHHH
TNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGPSQGKTFECKDGRKINVINHVDNNA
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCEEECCCCCEEEEEEECCCCH
FFDYITALAKKVN
HHHHHHHHHHHCC
>Mature Secondary Structure 
TKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEPSLSASVKIINRFSNED
CCEEEECCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCEEECCCCCHHHHHHHHCCCCC
IQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNVSDKEAFEDIIQTLKRQSE
EEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
KVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPKGNVEEPEHDGSAEWNAYWDP
EEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECC
EAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANERQYTGIDFLGVSYAAVPPLTHFI
CEEEEEECCCCEEEEEEECCCCCCEEEEEHHHHHCCCCCCCCEEEECCCHHHHCHHHHHH
TNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGPSQGKTFECKDGRKINVINHVDNNA
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCEEECCCCCEEEEEEECCCCH
FFDYITALAKKVN
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA