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The map label for this gene is pyrG

Identifier: 88196031

GI number: 88196031

Start: 2190059

End: 2191669

Strand: Reverse

Name: pyrG

Synonym: SAOUHSC_02368

Alternate gene names: 88196031

Gene position: 2191669-2190059 (Counterclockwise)

Preceding gene: 88196032

Following gene: 88196029

Centisome position: 77.68

GC content: 33.83

Gene sequence:

>1611_bases
ATGACAAAATTTATTTTTGTAACAGGTGGCGTAGTTTCATCATTAGGGAAGGGTATTACAGCATCTTCTCTAGGTAGATT
ATTAAAAGATAGAGGTCTAAATGTAACAATTCAAAAATTCGATCCATACTTAAATGTTGACCCAGGTACAATGAGTCCTT
ATCAACATGGTGAAGTATTCGTAACGGATGATGGTGCAGAAACTGACCTAGACTTAGGACATTACGAAAGATTTATTGAT
ATTAATTTAAACAAGTTTTCAAATGTGACAGCCGGTAAAGTGTATTCACACGTATTGAAAAAAGAACGTCGTGGTGATTA
CTTAGGCGGAACAGTTCAAGTTATTCCGCATATTACAAATGAAATTAAAGAACGTTTATTACTTGCAGGGGAAAGTACGA
ATGCAGACGTTGTTATCACTGAAATTGGCGGTACAACAGGTGATATTGAGTCATTACCGTTTATTGAAGCGATTCGTCAA
ATTCGTAGCGATTTAGGTAGAGAAAATGTTATGTATGTTCACTGTACATTACTGCCTTATATTAAAGCTGCTGGAGAAAT
GAAAACGAAGCCAACACAACATAGTGTTAAAGAATTACGAGGCTTAGGTATTCAACCAGACTTAATCGTTGTAAGAACTG
AATATGAAATGACACAAGATTTAAAAGATAAAATTGCATTATTCTGTGACATTAATAAAGAAAGTGTTATTGAATGTCGT
GATGCAGACTCTTTATACGAAATTCCATTACAATTAAGCCAACAAAATATGGATGATATCGTTATTAAACGTTTACAATT
AAACGCGAAATATGAAACACAGCTTGATGAATGGAAACAGTTGTTAGATATCGTTAATAATTTAGATGGTAAAATTACAA
TTGGTTTAGTAGGTAAATATGTTAGCTTACAAGATGCATATTTATCAGTTGTTGAATCATTGAAACATGCTGGATATCCT
TTTGCCAAAGATATTGACATTAGATGGATTGATTCAAGTGAAGTAACAGATGAAAATGCAGCCGAATACCTTGCAGATGT
CGACGGTATTTTAGTACCAGGTGGATTTGGTTTCCGTGCAAGTGAAGGTAAAATTAGTGCAATTAAGTATGCTAGAGAAA
ACAATGTACCATTCTTTGGTATTTGTTTAGGAATGCAACTTGCAACAGTTGAATTTTCAAGAAACGTATTAGGCCTTGAA
GGCGCACATTCAGCTGAATTAGACCCAGCAACACCATACCCAATTATAGATTTATTACCAGAACAAAAAGATATCGAAGA
TTTAGGTGGTACATTACGCTTAGGCTTATATTCATGTTCAATTAAAGAAGGCACATTGGCACAAGATGTTTATGGTAAAG
CGGAAATTGAAGAAAGACATCGTCATCGTTATGAATTTAATAATGACTATAGAGAACAATTAGAAGCAAATGGTATGGTG
ATTTCTGGTACAAGTCCAGATGGACGTTTAGTAGAAATGGTAGAGATTCCGACAAATGATTTCTTTATTGCTTGTCAATT
CCACCCAGAATTCTTATCTAGACCAAATCGTCCGCACCCGATTTTTAAATCATTTATTGAAGCTTCATTAAAATATCAAC
AAAATAAATAA

Upstream 100 bases:

>100_bases
ACAAATAAACAACTTGAATGAGCTTGCTTTAATGTTATGTTTTACGTAATTTTTACAATTGATGAGGAAGCATTCCCTTT
AATAATTAGGAGGTCAAGAC

Downstream 100 bases:

>100_bases
ATTTGCTAATAAAACCGGTACTTTCATTGTTAAACATTGAAAGTACCGGTTTTTCGTATAATTTTAATATTATGTTAGTG
ACAAGGTATGAAATAACAAT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFID
INLNKFSNVTAGKVYSHVLKKERRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMTQDLKDKIALFCDINKESVIECR
DADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLDEWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYP
FAKDIDIRWIDSSEVTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEFSRNVLGLE
GAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYSCSIKEGTLAQDVYGKAEIEERHRHRYEFNNDYREQLEANGMV
ISGTSPDGRLVEMVEIPTNDFFIACQFHPEFLSRPNRPHPIFKSFIEASLKYQQNK

Sequences:

>Translated_536_residues
MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFID
INLNKFSNVTAGKVYSHVLKKERRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMTQDLKDKIALFCDINKESVIECR
DADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLDEWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYP
FAKDIDIRWIDSSEVTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEFSRNVLGLE
GAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYSCSIKEGTLAQDVYGKAEIEERHRHRYEFNNDYREQLEANGMV
ISGTSPDGRLVEMVEIPTNDFFIACQFHPEFLSRPNRPHPIFKSFIEASLKYQQNK
>Mature_535_residues
TKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDI
NLNKFSNVTAGKVYSHVLKKERRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQI
RSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMTQDLKDKIALFCDINKESVIECRD
ADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLDEWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPF
AKDIDIRWIDSSEVTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEFSRNVLGLEG
AHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYSCSIKEGTLAQDVYGKAEIEERHRHRYEFNNDYREQLEANGMVI
SGTSPDGRLVEMVEIPTNDFFIACQFHPEFLSRPNRPHPIFKSFIEASLKYQQNK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=46.1678832116788, Blast_Score=504, Evalue=1e-143,
Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=45.5210237659963, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=45.5210237659963, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=45.5210237659963, Blast_Score=493, Evalue=1e-139,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=53.0275229357798, Blast_Score=592, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=38.1426202321725, Blast_Score=419, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=42.530755711775, Blast_Score=472, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=42.8571428571429, Blast_Score=462, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24664469, Length=548, Percent_Identity=45.8029197080292, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI21357815, Length=493, Percent_Identity=43.6105476673428, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59983; Mature: 59852

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYLNVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
EIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQIRSDLGRENVMYVHCTLLPY
HHHHHHEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHH
IKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMTQDLKDKIALFCDINKESVIECR
HHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHCEEEEEECCCCCEEEEC
DADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLDEWKQLLDIVNNLDGKITIGLVGKY
CCCCHHHCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCH
VSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSEVTDENAAEYLADVDGILVPGGFGFRA
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEECCCCCCCC
SEGKISAIKYARENNVPFFGICLGMQLATVEFSRNVLGLEGAHSAELDPATPYPIIDLLP
CCCCEEEEEEHHCCCCCEEEEECCCEEEEEECCCCEECCCCCCCCCCCCCCCCCHHHCCC
EQKDIEDLGGTLRLGLYSCSIKEGTLAQDVYGKAEIEERHRHRYEFNNDYREQLEANGMV
CCCCHHHCCCEEEEEEEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCCEE
ISGTSPDGRLVEMVEIPTNDFFIACQFHPEFLSRPNRPHPIFKSFIEASLKYQQNK
EECCCCCCCEEEEEEECCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYLNVDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
EIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQIRSDLGRENVMYVHCTLLPY
HHHHHHEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHH
IKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMTQDLKDKIALFCDINKESVIECR
HHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHCEEEEEECCCCCEEEEC
DADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLDEWKQLLDIVNNLDGKITIGLVGKY
CCCCHHHCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCH
VSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSEVTDENAAEYLADVDGILVPGGFGFRA
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEECCCCCCCC
SEGKISAIKYARENNVPFFGICLGMQLATVEFSRNVLGLEGAHSAELDPATPYPIIDLLP
CCCCEEEEEEHHCCCCCEEEEECCCEEEEEECCCCEECCCCCCCCCCCCCCCCCHHHCCC
EQKDIEDLGGTLRLGLYSCSIKEGTLAQDVYGKAEIEERHRHRYEFNNDYREQLEANGMV
CCCCHHHCCCEEEEEEEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCCEE
ISGTSPDGRLVEMVEIPTNDFFIACQFHPEFLSRPNRPHPIFKSFIEASLKYQQNK
EECCCCCCCEEEEEEECCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA