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The map label for this gene is polA [H]

Identifier: 88195497

GI number: 88195497

Start: 1693829

End: 1696459

Strand: Reverse

Name: polA [H]

Synonym: SAOUHSC_01797

Alternate gene names: 88195497

Gene position: 1696459-1693829 (Counterclockwise)

Preceding gene: 88195498

Following gene: 88195496

Centisome position: 60.13

GC content: 32.23

Gene sequence:

>2631_bases
GTGAATAAATTAGTATTAATCGATGGTAATAGCTTAAGTTTTAGAGCTTTTTATGCATTACCTTTGTTATCAAACAAAGC
AGGCATTCATACCAATGCAGTATATGGTTTTGCAATGTTACTAGAGAAGATTTTAAAAGAAGAAAAGCCAAATCATTTTT
TAGTAGCGTTTGATGCAGGTAAAACGACTTTCAGACATGAAAAATATAGTGAATATAAAGGTGGACGCCAGAAAACGCCG
CCTGAACTAAGTGAGCAATTTCCTTATATTCGCCAATTATTAGATGCTTATCATATTAAACGTTATGAATTAGATAACTA
TGAGGCAGATGATATTATCGGAACTTTAAGTAAAGAAGCAGATAAAGCGGGCTTTCAGACAATTATTATTACGGGAGACC
GAGATTTAACACAACTTGCAACGGATAATGTAACCATTTATTACACTAAAAAAGGTGTTACTGATGTTGATCATTATACA
CCTGACTTTATTGCGGAAAAATACAACGGGTTAACACCTAATCAAATTATTGATATGAAAGGATTAATGGGAGATACTTC
TGATAATATACCAGGTGTTGCAGGTGTTGGAGAGAAAACAGCAATAAAATTGCTAAACCAATTTGACACAGTAGAAGGTG
TCTATGAACATTTAGATGAAATTTCAGGTAAAAAGTTAAAAGAAAAACTTCAAAATAGCAAAGAAGATGCATTAATGAGT
AAAGAATTAGCAACGATTAATGTAGATAGTCCGATTGAAGTAAAACTTGAAGATACGTTAATGACTCATCAAGATGAACA
ACAAGAAAAAATCGAATTGTTTAAGAAGTTAGAATTCAAACAATTGTTGGCTGACATTGATCAATCAGCAAGCGTTGAAG
ATGCAATAGAAAAGACATTTGAAATTGAAACGTCTTTTGATAATATTGATTTTACTTCATTGAAAGAAGCGGCCATCCAT
TTCGAATTAGACGGTGGTAACTATTTGCGAAATAATATTTTAAAATTCTCTTTATTTACAGGTGAGAAACATATTGTAAT
TAATGCGGATGACATAAATAATTATGTCGAACTAGTTTCATGGTTAGAAAATCCGAATTCGAAAAAAGTCGTATATGATG
CTAAAAAAACATATGTAGCATCACATAGATTGGGGATTGATATTCAAAATATTTCTTTCGATATTATGTTGGCCAGTTAT
ATAATTGATCCATCTCGTACGATTAGTGATGTTCAATCAGTTGTTTCATTGTACGGTCAAAGTTTTGTGAAAGACGATGT
GAGTATATATGGAAAAGGTAAGAAATTTAAGGTACCTGAAGATGATGTTTTAAATCCATATGTTGCTTCTATTACTGATG
CAATTTACTTTGCGAAACCAAATATGGATAAACAATTAGAAGAATACAATCAGGTAGAACTCTTGGCTGATTTAGAGCTA
CCGCTAGCTAAGATTTTAAGTGAAATGGAAGAAATTGGTATATTTACAGATGTTCATGATTTAGAAGAAATGGAAAAAGA
AATTCAAGAAAAATTAGACGTCTTGATTCGAAATATCCATGATGCAGCTGGTGAAGATTTTAATATAAATTCTCCTAAGC
AATTAGGTGTTGTATTGTTTGAGACATTACAATTACCTGTTATTAAAAAGACGAAAACAGGATATTCTACTGCTGTAGAT
GTCTTAGAGCAATTGCAAGGTGAACATCCTATCATTGATTATATTTTAGAATATCGTCAATTATCTAAGTTACAGTCTAC
TTATGTTGAAGGATTGCAAAAGGTAATTAGTGATGATCAACGTATCCATACACGTTTTAATCAAACTTTAGCGCAAACTG
GACGTTTATCAAGTGTAGATCCTAATTTACAAAATATTCCGGTTAGACTTGAAGAAGGGCGTAAAATAAGAAAAGCCTTT
AAACCAACTTCAAAAGATAGCGTTATATTATCAGCAGATTATTCTCAAATTGAATTGCGTGTATTAGCACACATTACACA
AGATGAGAGTATGAAAGAAGCATTTATCAACGGCGATGATATTCATACAGCAACTGCTATGAAAGTATTTGGTGTAGAAG
CTGATCAAGTCGATAGTTTAATGCGTCGTCAAGCAAAAGCAGTTAACTTTGGAATTGTTTATGGGATAAGTGATTATGGT
TTAAGTCAAAGTTTAGGTATTACTCGTAAAAAAGCAAAAGCATTCATTGATGATTATTTAGCTAGTTTCCCAGGTGTAAA
ACAATATATGTCTGATATTGTAAAAGATGCCAAAGCTTTAGGTTACGTGGAAACATTGCTACATCGTCGACGCTATATTC
CTGATATTACGAGTCGTAACTTTAATTTACGCGGCTTTGCTGAACGTACTGCTATGAATACGCCAATACAGGGCAGTGCT
GCAGATATCATTAAACTGGCAATGGTTAAATTTGCTCAAAAAATGAAAGAGACAACATATCAAGCTAAACTATTATTACA
AGTACACGATGAATTAATTTTTGAAGTACCTAAGTCAGAAGTAGATTCATTTAGTGAATTTGTAGAAGAGATAATGGAAA
ATGCATTGCAATTAGATGTTCCATTAAAAGTAGACTCAAGTTATGGTGCAACCTGGTATGATGCAAAATAA

Upstream 100 bases:

>100_bases
TTAGGTCGTGTATAAATACTCTATGAGATAGTTTCTTTAATTACGACATCACAGTCGAATATGCTAAAATATATATAAAT
TAGTTCTGGAGGTTAAAATT

Downstream 100 bases:

>100_bases
AGTGAGGTTAATGGTATGCCCGAATTACCAGAAGTAGAACATGTAAAAAGAGGAATTGAACCCTACGTTATAAATCAAAA
AATTGAACACGTTATATTTT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 876; Mature: 876

Protein sequence:

>876_residues
MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKILKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTP
PELSEQFPYIRQLLDAYHIKRYELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHLDEISGKKLKEKLQNSKEDALMS
KELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKKLEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIH
FELDGGNYLRNNILKFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNISFDIMLASY
IIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVASITDAIYFAKPNMDKQLEEYNQVELLADLEL
PLAKILSEMEEIGIFTDVHDLEEMEKEIQEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVD
VLEQLQGEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQNIPVRLEEGRKIRKAF
KPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIHTATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYG
LSQSLGITRKKAKAFIDDYLASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQGSA
ADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENALQLDVPLKVDSSYGATWYDAK

Sequences:

>Translated_876_residues
MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKILKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTP
PELSEQFPYIRQLLDAYHIKRYELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHLDEISGKKLKEKLQNSKEDALMS
KELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKKLEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIH
FELDGGNYLRNNILKFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNISFDIMLASY
IIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVASITDAIYFAKPNMDKQLEEYNQVELLADLEL
PLAKILSEMEEIGIFTDVHDLEEMEKEIQEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVD
VLEQLQGEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQNIPVRLEEGRKIRKAF
KPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIHTATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYG
LSQSLGITRKKAKAFIDDYLASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQGSA
ADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENALQLDVPLKVDSSYGATWYDAK
>Mature_876_residues
MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKILKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTP
PELSEQFPYIRQLLDAYHIKRYELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHLDEISGKKLKEKLQNSKEDALMS
KELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKKLEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIH
FELDGGNYLRNNILKFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNISFDIMLASY
IIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVASITDAIYFAKPNMDKQLEEYNQVELLADLEL
PLAKILSEMEEIGIFTDVHDLEEMEKEIQEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVD
VLEQLQGEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQNIPVRLEEGRKIRKAF
KPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIHTATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYG
LSQSLGITRKKAKAFIDDYLASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQGSA
ADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENALQLDVPLKVDSSYGATWYDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=515, Percent_Identity=32.0388349514563, Blast_Score=243, Evalue=7e-64,
Organism=Homo sapiens, GI139394648, Length=630, Percent_Identity=26.8253968253968, Blast_Score=220, Evalue=5e-57,
Organism=Escherichia coli, GI1790294, Length=925, Percent_Identity=38.4864864864865, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI226510970, Length=221, Percent_Identity=31.2217194570136, Blast_Score=126, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI86563272, Length=378, Percent_Identity=32.2751322751323, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17933644, Length=508, Percent_Identity=29.5275590551181, Blast_Score=224, Evalue=2e-58,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 99196; Mature: 99196

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKILKEEKPNHFLVAFDAG
CCEEEEEECCCCEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
KTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRYELDNYEADDIIGTLSKEA
CCCHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
DKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYTPDFIAEKYNGLTPNQIIDMK
HCCCCEEEEEECCCCHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHEEEHH
GLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHLDEISGKKLKEKLQNSKEDALMS
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
KELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKKLEFKQLLADIDQSASVEDAIEKTF
HHHEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHE
EIETSFDNIDFTSLKEAAIHFELDGGNYLRNNILKFSLFTGEKHIVINADDINNYVELVS
EEECCCCCCCHHHHCCEEEEEEECCCCHHHCCCEEEEEEECCEEEEEECCCHHHHHHHHH
WLENPNSKKVVYDAKKTYVASHRLGIDIQNISFDIMLASYIIDPSRTISDVQSVVSLYGQ
HHHCCCCCEEEEECHHHHHHHHHCCEEEECCCHHEEHHHHHCCCCHHHHHHHHHHHHHHH
SFVKDDVSIYGKGKKFKVPEDDVLNPYVASITDAIYFAKPNMDKQLEEYNQVELLADLEL
HHHCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHEEHHCCC
PLAKILSEMEEIGIFTDVHDLEEMEKEIQEKLDVLIRNIHDAAGEDFNINSPKQLGVVLF
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ETLQLPVIKKTKTGYSTAVDVLEQLQGEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQ
HHHCCCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
RIHTRFNQTLAQTGRLSSVDPNLQNIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELR
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCEEEECCCHHHHHH
VLAHITQDESMKEAFINGDDIHTATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYG
HHHHHHCCHHHHHHHCCCCCCHHHHHHHEECCCHHHHHHHHHHHHHHCCEEEEEEECCCC
LSQSLGITRKKAKAFIDDYLASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRN
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FNLRGFAERTAMNTPIQGSAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSE
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VDSFSEFVEEIMENALQLDVPLKVDSSYGATWYDAK
HHHHHHHHHHHHHHHHEECCCEEECCCCCCEECCCC
>Mature Secondary Structure
MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKILKEEKPNHFLVAFDAG
CCEEEEEECCCCEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
KTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRYELDNYEADDIIGTLSKEA
CCCHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
DKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYTPDFIAEKYNGLTPNQIIDMK
HCCCCEEEEEECCCCHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHEEEHH
GLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHLDEISGKKLKEKLQNSKEDALMS
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
KELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKKLEFKQLLADIDQSASVEDAIEKTF
HHHEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHE
EIETSFDNIDFTSLKEAAIHFELDGGNYLRNNILKFSLFTGEKHIVINADDINNYVELVS
EEECCCCCCCHHHHCCEEEEEEECCCCHHHCCCEEEEEEECCEEEEEECCCHHHHHHHHH
WLENPNSKKVVYDAKKTYVASHRLGIDIQNISFDIMLASYIIDPSRTISDVQSVVSLYGQ
HHHCCCCCEEEEECHHHHHHHHHCCEEEECCCHHEEHHHHHCCCCHHHHHHHHHHHHHHH
SFVKDDVSIYGKGKKFKVPEDDVLNPYVASITDAIYFAKPNMDKQLEEYNQVELLADLEL
HHHCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHEEHHCCC
PLAKILSEMEEIGIFTDVHDLEEMEKEIQEKLDVLIRNIHDAAGEDFNINSPKQLGVVLF
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ETLQLPVIKKTKTGYSTAVDVLEQLQGEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQ
HHHCCCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
RIHTRFNQTLAQTGRLSSVDPNLQNIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELR
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCEEEECCCHHHHHH
VLAHITQDESMKEAFINGDDIHTATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYG
HHHHHHCCHHHHHHHCCCCCCHHHHHHHEECCCHHHHHHHHHHHHHHCCEEEEEEECCCC
LSQSLGITRKKAKAFIDDYLASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRN
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FNLRGFAERTAMNTPIQGSAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSE
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VDSFSEFVEEIMENALQLDVPLKVDSSYGATWYDAK
HHHHHHHHHHHHHHHHEECCCEEECCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]