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The map label for this gene is gyrB [H]

Identifier: 87201322

GI number: 87201322

Start: 3529785

End: 3532292

Strand: Direct

Name: gyrB [H]

Synonym: Saro_3310

Alternate gene names: 87201322

Gene position: 3529785-3532292 (Clockwise)

Preceding gene: 87201319

Following gene: 87201323

Centisome position: 99.11

GC content: 65.19

Gene sequence:

>2508_bases
ATGGCCAGCACGAACGAAAACACCCCCAACACCAATTCCTACGGCGCGGATTCGATCAAGGTCCTGAAAGGCCTCGACGC
GGTGCGCAAGCGCCCCGGCATGTACATCGGCGACACAGACGACGGGTCGGGCCTGCACCACATGGTGTTCGAGGTGTCGG
ACAACGCCATCGACGAGGCGCTGGCCGGGCACTGCGACCTCGTGCTGATCGAACTGAATCCGGACGGGTCGGTCTCGGTC
GAGGACAACGGGCGCGGCATTCCCACCGACATCCACGCCGAAGAGGGCGTCTCGGCGGCCGAAGTCATCATGACCCAGCT
CCACGCAGGCGGAAAGTTCGAGAATACCTCGGACGACAACGCCTACAAGGTTTCGGGCGGCCTCCATGGCGTGGGCGTGT
CGGTGGTCAACGCGCTTTCGGAATGGCTGGAGCTGACGATCTGGCGCGACGGCAAGGAGCACTGGATGCGCTTCGCCCAT
GGTGACGCGGAAGGCCCGCTGGTCGTGCGCGGCCCCGCACCCGAGGGCAAGAAGGGCACCCGCGTGACCTTCCAGGCCAG
CCACGAGACCTTCAAGAACGTGCTCGAGTTCGATTTCGACAAGCTGGAGCACCGCTACCGCGAACTCGCCTTCCTCAACT
CGGGCGTCCGCATCCTGCTGCGCGACAAGCGGCACGAGGAAATCAAGGAACACGACCTGTTCTATGAAGGCGGGATCGCG
GCCTTCGTGAAGTACCTCGACCGCAACAAGCAGCCCCTGATGCCCGAGCCGGTGGCGATTTCGGCCAACCGCGACGGCAT
CGGCATCGACGTGGCGCTGGAGTGGAACGACAGCTACTACGAGAACGTCCTGTGCTTCACCAACAACATCCCCCAGCGTG
ACGGCGGCACCCATCTGGCGGCCTTCCGCGCCGCGTTGACGCGCACGCTGAACGGCTATGCCGAGCGTTCGGGCATGCTG
AAGAAGGAAAAGGTCTCGCTCACCGGCGACGACATGCGCGAGGGCCTGACGGCGATCGTCTCGGTCAAGCTGCCCGATCC
GAAGTTCTCTTCGCAGACCAAGGACAAGCTGGTCTCCTCCGAAGTGCGCCAGCCACTTGAAAGCCTGATGGCCGACAAGA
TGGTCGAATGGCTCGAGGAGAACCCCGGGCACGCGAAGTCGATCATACAGAAGGTGATCGACGCCGCCGCCGCGCGCGAA
GCGGCCAAGCGGGCGCGCGAACTGACGCGGCGCAAGGGCGCGATGGACATCGCCTCGCTGCCCGGCAAGCTCGCCGACTG
CCAGGAGCGCGATCCCGCCAAGTGCGAACTGTTCCTGGTCGAGGGTGACTCCGCAGGCGGATCGGCCAAGCAGGGACGCG
ACCGCAAGACGCAGGCGATCCTGCCCCTCAAGGGCAAGATCCTCAACGTCGAACGCGCGCGCTTCGACCGGATCATCTCG
TCGAAGGAAGTGGGCACGCTGATCCAGGCGATGGGCACGGGCCTGCGCGACGAGTTCAACCTGGAAAAGCTGCGCTATCA
CAAGATCGTGATCATGACCGACGCCGACGTCGACGGCGCGCACATCCGCACGCTCCTGCTCACGTTCTTCCATCGCCAGA
TGCCCGAAATCATCAAGGCCGGGCATCTCTTCATCGCCCAGCCACCGCTCTACAAGGTCGCCAAGGGCCGCAGCGAGGTC
TATCTCAAGGACAACGCCGCGATGGACCGCTACCTCGTCGAGGCGGGCCTTTCGGGACGCGTTCTGGAAACCAGTGGCGG
AGCGCGCAGCGGAACGGACCTCGAAGCGCTGGTCGAACATGCGCTGCGCATGCGCAATCTCATGGCATTTGTGCCGCGCC
GCTACAATCCGGCCATCATCGAGGCGCTCGCGCTGGCCGGCGCGCTGGAACCCGACATGACGGTCGAAGCGCGCGATGCG
GCGCTGCTGCGCGCCGCCGACTGGCTCAACCGCGGCGATACCGAGGCGCGTTGGACAGTCACGCTCGCCGAGGAAGGCGG
CTACCACGTGCAGCGCATGTGGCGCGGCGTGACCGATCACCACATGATCGAAGCCGGCTTCCTGGTCAGCGCGGAAGCGC
GCAAGCTGGCCAAGCTCGCCGCCGAGAACGCCGAAGTCTATGCCGGAATGGCAAGGCTGGTGCGGGCGACTGCAGCAGCT
TCCGAGGCGGATGCCGCCCCTGCCGCCGATGATGACGGCGAGGTGCCCGCAGCACCGACCGTGCAGCACAAGGACGCGAT
CACGCGGCCGTCCGAGCTGCTCGAGATGGTCTTTGCCGGTGGCCGCAAGGGCCTTTCGATCCAGCGCTACAAGGGCCTTG
GCGAGATGAACGCCGAGCAGCTCTGGGAAACCACGCTCGACCCGGACAACCGCTCGCTGCTGCAGGTCAAGGTCGAGGAC
GCGGACGTCACCGACGAGATCTTTACCCGCCTGATGGGCGACGTGGTCGAACCGCGCCGCGACTTCATCCAGGAAAACGC
GCTGAACGTGGCAAATCTGGACGTCTGA

Upstream 100 bases:

>100_bases
TTCCCGGGCAGGCCCGGACAAGGTCGCAAATGCGGCCTGCGCTGTGCAAAACGGGCCGGATTCGCTTGGGTTTTCGGCCG
CGAATGCCTATGTGCAAAAG

Downstream 100 bases:

>100_bases
TCTTGGCCTACACCGACGAAATTCCGTGGGACCAGCCCGCGACCATGATCGACCTCGAGGGCCGCGCGCCCATCATCGGC
ACGATCCGCGACTGCGCGCT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 835; Mature: 834

Protein sequence:

>835_residues
MASTNENTPNTNSYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLVLIELNPDGSVSV
EDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGKEHWMRFAH
GDAEGPLVVRGPAPEGKKGTRVTFQASHETFKNVLEFDFDKLEHRYRELAFLNSGVRILLRDKRHEEIKEHDLFYEGGIA
AFVKYLDRNKQPLMPEPVAISANRDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNGYAERSGML
KKEKVSLTGDDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADKMVEWLEENPGHAKSIIQKVIDAAAARE
AAKRARELTRRKGAMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRKTQAILPLKGKILNVERARFDRIIS
SKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPEIIKAGHLFIAQPPLYKVAKGRSEV
YLKDNAAMDRYLVEAGLSGRVLETSGGARSGTDLEALVEHALRMRNLMAFVPRRYNPAIIEALALAGALEPDMTVEARDA
ALLRAADWLNRGDTEARWTVTLAEEGGYHVQRMWRGVTDHHMIEAGFLVSAEARKLAKLAAENAEVYAGMARLVRATAAA
SEADAAPAADDDGEVPAAPTVQHKDAITRPSELLEMVFAGGRKGLSIQRYKGLGEMNAEQLWETTLDPDNRSLLQVKVED
ADVTDEIFTRLMGDVVEPRRDFIQENALNVANLDV

Sequences:

>Translated_835_residues
MASTNENTPNTNSYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLVLIELNPDGSVSV
EDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGKEHWMRFAH
GDAEGPLVVRGPAPEGKKGTRVTFQASHETFKNVLEFDFDKLEHRYRELAFLNSGVRILLRDKRHEEIKEHDLFYEGGIA
AFVKYLDRNKQPLMPEPVAISANRDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNGYAERSGML
KKEKVSLTGDDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADKMVEWLEENPGHAKSIIQKVIDAAAARE
AAKRARELTRRKGAMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRKTQAILPLKGKILNVERARFDRIIS
SKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPEIIKAGHLFIAQPPLYKVAKGRSEV
YLKDNAAMDRYLVEAGLSGRVLETSGGARSGTDLEALVEHALRMRNLMAFVPRRYNPAIIEALALAGALEPDMTVEARDA
ALLRAADWLNRGDTEARWTVTLAEEGGYHVQRMWRGVTDHHMIEAGFLVSAEARKLAKLAAENAEVYAGMARLVRATAAA
SEADAAPAADDDGEVPAAPTVQHKDAITRPSELLEMVFAGGRKGLSIQRYKGLGEMNAEQLWETTLDPDNRSLLQVKVED
ADVTDEIFTRLMGDVVEPRRDFIQENALNVANLDV
>Mature_834_residues
ASTNENTPNTNSYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLVLIELNPDGSVSVE
DNGRGIPTDIHAEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGKEHWMRFAHG
DAEGPLVVRGPAPEGKKGTRVTFQASHETFKNVLEFDFDKLEHRYRELAFLNSGVRILLRDKRHEEIKEHDLFYEGGIAA
FVKYLDRNKQPLMPEPVAISANRDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNGYAERSGMLK
KEKVSLTGDDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADKMVEWLEENPGHAKSIIQKVIDAAAAREA
AKRARELTRRKGAMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRKTQAILPLKGKILNVERARFDRIISS
KEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPEIIKAGHLFIAQPPLYKVAKGRSEVY
LKDNAAMDRYLVEAGLSGRVLETSGGARSGTDLEALVEHALRMRNLMAFVPRRYNPAIIEALALAGALEPDMTVEARDAA
LLRAADWLNRGDTEARWTVTLAEEGGYHVQRMWRGVTDHHMIEAGFLVSAEARKLAKLAAENAEVYAGMARLVRATAAAS
EADAAPAADDDGEVPAAPTVQHKDAITRPSELLEMVFAGGRKGLSIQRYKGLGEMNAEQLWETTLDPDNRSLLQVKVEDA
DVTDEIFTRLMGDVVEPRRDFIQENALNVANLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=576, Percent_Identity=25.3472222222222, Blast_Score=140, Evalue=7e-33,
Organism=Homo sapiens, GI19913406, Length=557, Percent_Identity=24.4165170556553, Blast_Score=127, Evalue=5e-29,
Organism=Escherichia coli, GI48994957, Length=836, Percent_Identity=57.5358851674641, Blast_Score=882, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=553, Percent_Identity=40.6871609403255, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17535065, Length=541, Percent_Identity=26.2476894639556, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=505, Percent_Identity=25.5445544554455, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=144, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=598, Percent_Identity=24.5819397993311, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17136538, Length=535, Percent_Identity=26.1682242990654, Blast_Score=142, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91942; Mature: 91811

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTNENTPNTNSYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEECCHHHHHH
LAGHCDLVLIELNPDGSVSVEDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGKFENTSDDN
HCCCCCEEEEEECCCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCC
AYKVSGGLHGVGVSVVNALSEWLELTIWRDGKEHWMRFAHGDAEGPLVVRGPAPEGKKGT
CEEECCCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCEEEECCCCCCCCCC
RVTFQASHETFKNVLEFDFDKLEHRYRELAFLNSGVRILLRDKRHEEIKEHDLFYEGGIA
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCHH
AFVKYLDRNKQPLMPEPVAISANRDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLA
HHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEEECCCHHHCCEEEEECCCCCCCCCCHHH
AFRAALTRTLNGYAERSGMLKKEKVSLTGDDMREGLTAIVSVKLPDPKFSSQTKDKLVSS
HHHHHHHHHHHHHHHHCCCCCHHEECCCCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHH
EVRQPLESLMADKMVEWLEENPGHAKSIIQKVIDAAAAREAAKRARELTRRKGAMDIASL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
PGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRKTQAILPLKGKILNVERARFDRIIS
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHH
SKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPEIIKA
HHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GHLFIAQPPLYKVAKGRSEVYLKDNAAMDRYLVEAGLSGRVLETSGGARSGTDLEALVEH
CCEEEECCCHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHH
ALRMRNLMAFVPRRYNPAIIEALALAGALEPDMTVEARDAALLRAADWLNRGDTEARWTV
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEE
TLAEEGGYHVQRMWRGVTDHHMIEAGFLVSAEARKLAKLAAENAEVYAGMARLVRATAAA
EEECCCCHHHHHHHCCCCHHHEECCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
SEADAAPAADDDGEVPAAPTVQHKDAITRPSELLEMVFAGGRKGLSIQRYKGLGEMNAEQ
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHH
LWETTLDPDNRSLLQVKVEDADVTDEIFTRLMGDVVEPRRDFIQENALNVANLDV
HHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
ASTNENTPNTNSYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEECCHHHHHH
LAGHCDLVLIELNPDGSVSVEDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGKFENTSDDN
HCCCCCEEEEEECCCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCC
AYKVSGGLHGVGVSVVNALSEWLELTIWRDGKEHWMRFAHGDAEGPLVVRGPAPEGKKGT
CEEECCCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCEEEECCCCCCCCCC
RVTFQASHETFKNVLEFDFDKLEHRYRELAFLNSGVRILLRDKRHEEIKEHDLFYEGGIA
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCHH
AFVKYLDRNKQPLMPEPVAISANRDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLA
HHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEEECCCHHHCCEEEEECCCCCCCCCCHHH
AFRAALTRTLNGYAERSGMLKKEKVSLTGDDMREGLTAIVSVKLPDPKFSSQTKDKLVSS
HHHHHHHHHHHHHHHHCCCCCHHEECCCCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHH
EVRQPLESLMADKMVEWLEENPGHAKSIIQKVIDAAAAREAAKRARELTRRKGAMDIASL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
PGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRKTQAILPLKGKILNVERARFDRIIS
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHH
SKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPEIIKA
HHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GHLFIAQPPLYKVAKGRSEVYLKDNAAMDRYLVEAGLSGRVLETSGGARSGTDLEALVEH
CCEEEECCCHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHH
ALRMRNLMAFVPRRYNPAIIEALALAGALEPDMTVEARDAALLRAADWLNRGDTEARWTV
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEE
TLAEEGGYHVQRMWRGVTDHHMIEAGFLVSAEARKLAKLAAENAEVYAGMARLVRATAAA
EEECCCCHHHHHHHCCCCHHHEECCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
SEADAAPAADDDGEVPAAPTVQHKDAITRPSELLEMVFAGGRKGLSIQRYKGLGEMNAEQ
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHH
LWETTLDPDNRSLLQVKVEDADVTDEIFTRLMGDVVEPRRDFIQENALNVANLDV
HHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]