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The map label for this gene is folD

Identifier: 87201312

GI number: 87201312

Start: 3519973

End: 3520866

Strand: Direct

Name: folD

Synonym: Saro_3300

Alternate gene names: 87201312

Gene position: 3519973-3520866 (Clockwise)

Preceding gene: 87201311

Following gene: 87201313

Centisome position: 98.83

GC content: 67.34

Gene sequence:

>894_bases
ATGACTGCTGCCGTTATTGACGGAAAGGCCTTTGCCGCCACGCTGCGCGGGCGCGTCGGCGACCTTGCCACTGCATTCGA
GGCCAGGGCCGGTCGCAAGGCGGGGCTTGCCGTGGTGCTGGTGGGCGAGGACCCGGCGAGCCAAGTCTATGTTCGCTCGA
AGGGCAAGAGCACGCTCGAAGCGGGCATGGCAAGCTTCGAGCACAAGCTTCCCGCCGACACCGCCGAGGCCGACCTCCTT
GCCGTAGTGGAGCAGCTCAACGCCGATCCGGCGGTTGACGGCATTCTCGTCCAGCTTCCCCTGCCCCGGCACATGGACGA
GCAGAAGGTCATCGCCGCGATCAATCCCGACAAGGACGTCGATGGCTTCCACGTGATCAACGCGGGCCGCCTCGCCGTCG
GCCAGCCGGGCTTCGTGCCCTGCACCCCGCTTGGCTGCCTGATGTTGCTAAAGGACCGACTGGGCGACCTTTCCGGCCTC
GATGCCGTGGTAATCGGCCGCTCAAACATCGTCGGCAAGCCGATGGCGCAGTTGCTCATCGCCGAAAGCTGCACCGTCAC
GGTCGCGCACAGCCGCACGAAGAACCTCCCGGAAGTCGTGCGCCGCGCGGACATCGTCGTGGCAGCCGTAGGCCGCCCCG
AGATGGTCAAGGGCGACTGGATCAAGCCGGGCGCAACCGTGATCGACGTCGGCATCAACCGCGTCCCGGGCAAGGAAGAA
GGCAAGACCCGGCTGGTCGGCGACGTCGACTATGCGTCCGCCGCCGAAGTCGCGTCGGCCATCACCCCGGTTCCGGGCGG
CGTCGGCCCGATGACCATCGCCGTGCTTCTGCGCAACGCGCTGGTCGCGGCCTACCGCAACGCGGGGATCGACCTTTCCG
CCGACGCGATCTGA

Upstream 100 bases:

>100_bases
GGTGCTGATCATCGGCCTCAACCTGCTGACGATCCTGCTGAGCGGCGTTGCCGCCTCGGGCGCGGGGCTCTGAAGCCACA
CCGGAACGGAACCTATTTCG

Downstream 100 bases:

>100_bases
GCTTGGGCCGATCTGGACTTGGGCCGATCTGAAAATGGGCCGATCTGAAATTGGGTATGCGGTCCTGCGCGGGCCGCGCC
CTCAAATTCGCTGTCCTACA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTAAVIDGKAFAATLRGRVGDLATAFEARAGRKAGLAVVLVGEDPASQVYVRSKGKSTLEAGMASFEHKLPADTAEADLL
AVVEQLNADPAVDGILVQLPLPRHMDEQKVIAAINPDKDVDGFHVINAGRLAVGQPGFVPCTPLGCLMLLKDRLGDLSGL
DAVVIGRSNIVGKPMAQLLIAESCTVTVAHSRTKNLPEVVRRADIVVAAVGRPEMVKGDWIKPGATVIDVGINRVPGKEE
GKTRLVGDVDYASAAEVASAITPVPGGVGPMTIAVLLRNALVAAYRNAGIDLSADAI

Sequences:

>Translated_297_residues
MTAAVIDGKAFAATLRGRVGDLATAFEARAGRKAGLAVVLVGEDPASQVYVRSKGKSTLEAGMASFEHKLPADTAEADLL
AVVEQLNADPAVDGILVQLPLPRHMDEQKVIAAINPDKDVDGFHVINAGRLAVGQPGFVPCTPLGCLMLLKDRLGDLSGL
DAVVIGRSNIVGKPMAQLLIAESCTVTVAHSRTKNLPEVVRRADIVVAAVGRPEMVKGDWIKPGATVIDVGINRVPGKEE
GKTRLVGDVDYASAAEVASAITPVPGGVGPMTIAVLLRNALVAAYRNAGIDLSADAI
>Mature_296_residues
TAAVIDGKAFAATLRGRVGDLATAFEARAGRKAGLAVVLVGEDPASQVYVRSKGKSTLEAGMASFEHKLPADTAEADLLA
VVEQLNADPAVDGILVQLPLPRHMDEQKVIAAINPDKDVDGFHVINAGRLAVGQPGFVPCTPLGCLMLLKDRLGDLSGLD
AVVIGRSNIVGKPMAQLLIAESCTVTVAHSRTKNLPEVVRRADIVVAAVGRPEMVKGDWIKPGATVIDVGINRVPGKEEG
KTRLVGDVDYASAAEVASAITPVPGGVGPMTIAVLLRNALVAAYRNAGIDLSADAI

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=46.551724137931, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=42.5170068027211, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=43.1972789115646, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI36796743, Length=214, Percent_Identity=28.0373831775701, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=53.8732394366197, Blast_Score=277, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=45.9183673469388, Blast_Score=227, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=48.9583333333333, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=44.5182724252492, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=47.7508650519031, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=47.7508650519031, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=47.7508650519031, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=47.7508650519031, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=46.1016949152542, Blast_Score=247, Evalue=8e-66,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=46.1016949152542, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_NOVAD (Q2G338)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498569.1
- ProteinModelPortal:   Q2G338
- SMR:   Q2G338
- STRING:   Q2G338
- GeneID:   3915947
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_3300
- NMPDR:   fig|48935.1.peg.3892
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   Q2G338
- ProtClustDB:   PRK14188
- BioCyc:   NARO279238:SARO_3300-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30785; Mature: 30654

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAVIDGKAFAATLRGRVGDLATAFEARAGRKAGLAVVLVGEDPASQVYVRSKGKSTLE
CCEEEECCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCHHHH
AGMASFEHKLPADTAEADLLAVVEQLNADPAVDGILVQLPLPRHMDEQKVIAAINPDKDV
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCC
DGFHVINAGRLAVGQPGFVPCTPLGCLMLLKDRLGDLSGLDAVVIGRSNIVGKPMAQLLI
CCEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHH
AESCTVTVAHSRTKNLPEVVRRADIVVAAVGRPEMVKGDWIKPGATVIDVGINRVPGKEE
HCCCEEEEECCCCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GKTRLVGDVDYASAAEVASAITPVPGGVGPMTIAVLLRNALVAAYRNAGIDLSADAI
CCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAAVIDGKAFAATLRGRVGDLATAFEARAGRKAGLAVVLVGEDPASQVYVRSKGKSTLE
CEEEECCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCHHHH
AGMASFEHKLPADTAEADLLAVVEQLNADPAVDGILVQLPLPRHMDEQKVIAAINPDKDV
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCC
DGFHVINAGRLAVGQPGFVPCTPLGCLMLLKDRLGDLSGLDAVVIGRSNIVGKPMAQLLI
CCEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHH
AESCTVTVAHSRTKNLPEVVRRADIVVAAVGRPEMVKGDWIKPGATVIDVGINRVPGKEE
HCCCEEEEECCCCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GKTRLVGDVDYASAAEVASAITPVPGGVGPMTIAVLLRNALVAAYRNAGIDLSADAI
CCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA