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The map label for this gene is cysD [H]

Identifier: 87201264

GI number: 87201264

Start: 3471830

End: 3472744

Strand: Direct

Name: cysD [H]

Synonym: Saro_3252

Alternate gene names: 87201264

Gene position: 3471830-3472744 (Clockwise)

Preceding gene: 87201260

Following gene: 87201265

Centisome position: 97.48

GC content: 64.92

Gene sequence:

>915_bases
GTGACACCTGACCGCAGTACCCTGACGCATCTCCAGCGCCTCGAATCCGAAGCGATCCACATCATGCGCGAGGTCGTGGC
CGAGGCCGAGAACCCCGTGATGCTCTATTCGGTGGGCAAGGACAGCGCGGTGATGCTGCACCTCGCACGCAAGGCTTTCT
ACCCCTCGCCGCCGCCGTTCCCGCTGCTCCACGTCGACACCACGTGGAAGTTCAGGGCGATGTACGACCTGCGCGACCGC
ATGGCGAAGGAGAGCGGCATGGAGCTGCTCGTCTATCAGAATCCCGAGGCGGCCGAGCGCGGCATCAACCCGTTCGACCA
CGGCGCGCTCCACACCGACATGTGGAAGACCGAAGGGCTCAAGCAGGCGCTCGACAAGTGGGGCTTCGACGCGGCCTTCG
GCGGCGCACGCCGCGACGAGGAGAAGAGCCGCGCCAAGGAACGCATCTTCTCGTTCCGCACCGCCTCGCACGGCTGGGAC
CCCAAGAACCAGCGGCCCGAGCTGTGGAACCTCTACAATGCCCGCAAGAACAAGGGCGAGAGCATCCGCGTCTTCCCGAT
CTCGAACTGGACCGAGCTCGACATCTGGCAGTACATCCATCTCAACGACGTGCCGATCGTCCCGCTCTACTTCGCGGAAG
AACGTCCCACGGTCGAGCGCGACGGCATGCTGCTGATGGTCGACGACGACCGCTTCCCGCTCAAGCCGGGCGAGACGCCG
CAGATGCGTTCGATCCGTTTCCGCACGCTCGGCTGCTATCCGCTGACCGGCGCCGTGGAAAGCACCGCCAAGACGCTTCC
GGAAGTGATCCAGGAAACCCTGCTCACCACCACCAGCGAACGCCAGGGCCGCGCAATCGACAAGGACGCCGGCGGCGCGG
GCATGGAAGTCAAGAAGCAGCAGGGGTATTTCTGA

Upstream 100 bases:

>100_bases
AGCGCCTCCAAGATTTCCATCCGTGCGCGCTCCCATCTGGGAGCGCAAGAAGGCCGCGCGATTCGCCAGACGGGGCCAGC
GCAATACCGGAGCGAGAACC

Downstream 100 bases:

>100_bases
TGACCGACACGATCACCACGGAACAGCCCGTCTACGTCACCGACGCACTCATCGCCGAGGACATCGACCAGTACCTCAAG
GTGCATGAGCACAAGACGAT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTPDRSTLTHLQRLESEAIHIMREVVAEAENPVMLYSVGKDSAVMLHLARKAFYPSPPPFPLLHVDTTWKFRAMYDLRDR
MAKESGMELLVYQNPEAAERGINPFDHGALHTDMWKTEGLKQALDKWGFDAAFGGARRDEEKSRAKERIFSFRTASHGWD
PKNQRPELWNLYNARKNKGESIRVFPISNWTELDIWQYIHLNDVPIVPLYFAEERPTVERDGMLLMVDDDRFPLKPGETP
QMRSIRFRTLGCYPLTGAVESTAKTLPEVIQETLLTTTSERQGRAIDKDAGGAGMEVKKQQGYF

Sequences:

>Translated_304_residues
MTPDRSTLTHLQRLESEAIHIMREVVAEAENPVMLYSVGKDSAVMLHLARKAFYPSPPPFPLLHVDTTWKFRAMYDLRDR
MAKESGMELLVYQNPEAAERGINPFDHGALHTDMWKTEGLKQALDKWGFDAAFGGARRDEEKSRAKERIFSFRTASHGWD
PKNQRPELWNLYNARKNKGESIRVFPISNWTELDIWQYIHLNDVPIVPLYFAEERPTVERDGMLLMVDDDRFPLKPGETP
QMRSIRFRTLGCYPLTGAVESTAKTLPEVIQETLLTTTSERQGRAIDKDAGGAGMEVKKQQGYF
>Mature_303_residues
TPDRSTLTHLQRLESEAIHIMREVVAEAENPVMLYSVGKDSAVMLHLARKAFYPSPPPFPLLHVDTTWKFRAMYDLRDRM
AKESGMELLVYQNPEAAERGINPFDHGALHTDMWKTEGLKQALDKWGFDAAFGGARRDEEKSRAKERIFSFRTASHGWDP
KNQRPELWNLYNARKNKGESIRVFPISNWTELDIWQYIHLNDVPIVPLYFAEERPTVERDGMLLMVDDDRFPLKPGETPQ
MRSIRFRTLGCYPLTGAVESTAKTLPEVIQETLLTTTSERQGRAIDKDAGGAGMEVKKQQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=71.523178807947, Blast_Score=453, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34901; Mature: 34770

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDRSTLTHLQRLESEAIHIMREVVAEAENPVMLYSVGKDSAVMLHLARKAFYPSPPPF
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCC
PLLHVDTTWKFRAMYDLRDRMAKESGMELLVYQNPEAAERGINPFDHGALHTDMWKTEGL
CEEEECCCEEEEHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHH
KQALDKWGFDAAFGGARRDEEKSRAKERIFSFRTASHGWDPKNQRPELWNLYNARKNKGE
HHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
SIRVFPISNWTELDIWQYIHLNDVPIVPLYFAEERPTVERDGMLLMVDDDRFPLKPGETP
EEEEEECCCCCEECEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCC
QMRSIRFRTLGCYPLTGAVESTAKTLPEVIQETLLTTTSERQGRAIDKDAGGAGMEVKKQ
CHHEEEEEEECCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHC
QGYF
CCCC
>Mature Secondary Structure 
TPDRSTLTHLQRLESEAIHIMREVVAEAENPVMLYSVGKDSAVMLHLARKAFYPSPPPF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCC
PLLHVDTTWKFRAMYDLRDRMAKESGMELLVYQNPEAAERGINPFDHGALHTDMWKTEGL
CEEEECCCEEEEHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHH
KQALDKWGFDAAFGGARRDEEKSRAKERIFSFRTASHGWDPKNQRPELWNLYNARKNKGE
HHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
SIRVFPISNWTELDIWQYIHLNDVPIVPLYFAEERPTVERDGMLLMVDDDRFPLKPGETP
EEEEEECCCCCEECEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCC
QMRSIRFRTLGCYPLTGAVESTAKTLPEVIQETLLTTTSERQGRAIDKDAGGAGMEVKKQ
CHHEEEEEEECCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHC
QGYF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]