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The map label for this gene is hipO [H]

Identifier: 87200944

GI number: 87200944

Start: 3147585

End: 3148733

Strand: Reverse

Name: hipO [H]

Synonym: Saro_2932

Alternate gene names: 87200944

Gene position: 3148733-3147585 (Counterclockwise)

Preceding gene: 87200945

Following gene: 87200937

Centisome position: 88.41

GC content: 68.49

Gene sequence:

>1149_bases
ATGAGCGAAATCGCAGATCTCCTTCCGGACCTCGTGGCCCTGCGCCGCGACATCCATCAGAACCCCGAACTCGGCTTCTG
CGAACACCGCACCGCCGCGCGCATCGCCGCAGAACTGCGGGCCAGCGGGATCGAGGTCCACGAAGGCATCGGCACCACCG
GGCTCGTCGGCGTGCTCAAGGGCACGCGCGAGGGCACTCGCAGCCTCGGTCTGCGCGCCGACATGGATGCCCTGCCGATC
CACGAGCAGACCAATCTTCCCTGGGCAAGCCGTACCCCCGGCACCTTCCACGGCTGCGGCCACGACGGCCACGTCGCGAT
GCTGCTCGGCGCCGCCCGCGTGCTGGCTGCCGACCCCGACTTCGCGGGTACGGTCAACTTCATCTTCCAGCCCGCCGAGG
AGGGGCAGGGCGGGGCGCGCGTGATGATCGAGGAAGGCCTGTTCGACCGCTTCCCCTGCGACCGCGTCTATGCGCTGCAC
AACTGGCCGGGCCTGCCCGCCGGCACGATCAGCACCCGTCCCGGCGCGATCATGGGCGCGGCCGACAAGTTCCGCATCGC
GCTTGTCGGCAAGGGCGGCCACGCCGCGATTCCGCAGGACAGCCCCGATGCGATCCTTGCCGCCGCCAGCCTTGTCCAGC
AACTCAACACCATCGTCAGCCGTGCCGTGCCGCCCTATGCGGCGGCCGTCCTCTCGATCACCGAGATCCACGGTGGCCAC
GCCCACAACGTCATCCCGGCGGAAGTCATGGTGGGGGGCACGGTCCGCACGTTCGACCCGGCGGTCCAGGATCGCATCGA
GGAACGGATGCGCGCGATGCTGAAAGGCATCGAGGCTTCGTTCGAGGTCGAATGCGCGCTCGACTACGACCGTTACTATC
CCGCCACGATCAACGATCCGCAGGCGGCAGCCGATGCGCTGGAAGTGGCCGCGACGGTGGCACGGGCGGAACTCGCGCCT
GAACCCGCGCCGACCTCGGAAGACTTTTCCTTCATGCTCCAGCAGCGCCCCGGCGCCTACATCTGGCTGGGGCAGGGCAC
GCAGGGCCACGCCGCGCCGCTCCACAATCCACACTACGATTTCAACGATGGCGTCATGGAAACGGGCATCCGTCTCCACG
TTGCGCTTGCCCGGCACTGGCTGGGCTGA

Upstream 100 bases:

>100_bases
CCTTCATCAAGCACCACTTCCCGATCCAGAACCTCTTCGGCCAGACCCACGGCTGAAGCCTCAAGGCCAAGGCAACCTTG
CTGACGGACCCAAGGACAGC

Downstream 100 bases:

>100_bases
AACGGGGACGGCCCGGATCGATCCGGGCCGCTGGCAGCTAGAAGCTAGAGCCTAGAAGCGCAGGGCCAGCCCGACGATGG
CGCGATTGATACTGCCGCGG

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSEIADLLPDLVALRRDIHQNPELGFCEHRTAARIAAELRASGIEVHEGIGTTGLVGVLKGTREGTRSLGLRADMDALPI
HEQTNLPWASRTPGTFHGCGHDGHVAMLLGAARVLAADPDFAGTVNFIFQPAEEGQGGARVMIEEGLFDRFPCDRVYALH
NWPGLPAGTISTRPGAIMGAADKFRIALVGKGGHAAIPQDSPDAILAAASLVQQLNTIVSRAVPPYAAAVLSITEIHGGH
AHNVIPAEVMVGGTVRTFDPAVQDRIEERMRAMLKGIEASFEVECALDYDRYYPATINDPQAAADALEVAATVARAELAP
EPAPTSEDFSFMLQQRPGAYIWLGQGTQGHAAPLHNPHYDFNDGVMETGIRLHVALARHWLG

Sequences:

>Translated_382_residues
MSEIADLLPDLVALRRDIHQNPELGFCEHRTAARIAAELRASGIEVHEGIGTTGLVGVLKGTREGTRSLGLRADMDALPI
HEQTNLPWASRTPGTFHGCGHDGHVAMLLGAARVLAADPDFAGTVNFIFQPAEEGQGGARVMIEEGLFDRFPCDRVYALH
NWPGLPAGTISTRPGAIMGAADKFRIALVGKGGHAAIPQDSPDAILAAASLVQQLNTIVSRAVPPYAAAVLSITEIHGGH
AHNVIPAEVMVGGTVRTFDPAVQDRIEERMRAMLKGIEASFEVECALDYDRYYPATINDPQAAADALEVAATVARAELAP
EPAPTSEDFSFMLQQRPGAYIWLGQGTQGHAAPLHNPHYDFNDGVMETGIRLHVALARHWLG
>Mature_381_residues
SEIADLLPDLVALRRDIHQNPELGFCEHRTAARIAAELRASGIEVHEGIGTTGLVGVLKGTREGTRSLGLRADMDALPIH
EQTNLPWASRTPGTFHGCGHDGHVAMLLGAARVLAADPDFAGTVNFIFQPAEEGQGGARVMIEEGLFDRFPCDRVYALHN
WPGLPAGTISTRPGAIMGAADKFRIALVGKGGHAAIPQDSPDAILAAASLVQQLNTIVSRAVPPYAAAVLSITEIHGGHA
HNVIPAEVMVGGTVRTFDPAVQDRIEERMRAMLKGIEASFEVECALDYDRYYPATINDPQAAADALEVAATVARAELAPE
PAPTSEDFSFMLQQRPGAYIWLGQGTQGHAAPLHNPHYDFNDGVMETGIRLHVALARHWLG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=422, Percent_Identity=30.3317535545024, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 40862; Mature: 40731

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIADLLPDLVALRRDIHQNPELGFCEHRTAARIAAELRASGIEVHEGIGTTGLVGVLK
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHH
GTREGTRSLGLRADMDALPIHEQTNLPWASRTPGTFHGCGHDGHVAMLLGAARVLAADPD
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCC
FAGTVNFIFQPAEEGQGGARVMIEEGLFDRFPCDRVYALHNWPGLPAGTISTRPGAIMGA
CCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEC
ADKFRIALVGKGGHAAIPQDSPDAILAAASLVQQLNTIVSRAVPPYAAAVLSITEIHGGH
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
AHNVIPAEVMVGGTVRTFDPAVQDRIEERMRAMLKGIEASFEVECALDYDRYYPATINDP
CCCCCCHHEEECCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCH
QAAADALEVAATVARAELAPEPAPTSEDFSFMLQQRPGAYIWLGQGTQGHAAPLHNPHYD
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCC
FNDGVMETGIRLHVALARHWLG
CCCCHHHCCCEEEHHHHHHHCC
>Mature Secondary Structure 
SEIADLLPDLVALRRDIHQNPELGFCEHRTAARIAAELRASGIEVHEGIGTTGLVGVLK
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHH
GTREGTRSLGLRADMDALPIHEQTNLPWASRTPGTFHGCGHDGHVAMLLGAARVLAADPD
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCC
FAGTVNFIFQPAEEGQGGARVMIEEGLFDRFPCDRVYALHNWPGLPAGTISTRPGAIMGA
CCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEC
ADKFRIALVGKGGHAAIPQDSPDAILAAASLVQQLNTIVSRAVPPYAAAVLSITEIHGGH
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
AHNVIPAEVMVGGTVRTFDPAVQDRIEERMRAMLKGIEASFEVECALDYDRYYPATINDP
CCCCCCHHEEECCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCH
QAAADALEVAATVARAELAPEPAPTSEDFSFMLQQRPGAYIWLGQGTQGHAAPLHNPHYD
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCC
FNDGVMETGIRLHVALARHWLG
CCCCHHHCCCEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]