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The map label for this gene is pchF [H]

Identifier: 87200889

GI number: 87200889

Start: 3098651

End: 3100237

Strand: Reverse

Name: pchF [H]

Synonym: Saro_2876

Alternate gene names: 87200889

Gene position: 3100237-3098651 (Counterclockwise)

Preceding gene: 87200890

Following gene: 87200888

Centisome position: 87.05

GC content: 65.66

Gene sequence:

>1587_bases
GTGAAGCTGCACCTGCCCCCGCGCGTCAGTCCGAAGCAGTTCCAGTCCGCGATGACCGCCTTTGCCGGCGTCGTCGGCAA
GGCCTGGGTCATGGACTCCGACGCCGACCGCGATGCCTATGCCGACACCTACGCGCCCGGCAGCACCGAGGAGTGGCCCG
CATCGGCTGCCGTCGCCCCGGCCAACGTCGAGGAAGTGCGCGCGATCGTGCGCCTTGCCAATGAGCACAAGGTGCCGCTC
TGGCCCGTCGCGCGCGGCAAGAACCTTGGCTACGGCACCGCTGCGCCGCGCATGGAAGGCACCGTGGTCATGGACCTCGG
CCGCATGAACAAGGTGCTCGAGCTTGACCACAAGCTCGCCTATTGCGTGGTCGAACCGGGCGTTGGCTTCTTCGACCTCT
ACGACCAGATCCAGCGCGAGAAGGCGCCGCTGTGGATGTCGGTGCCGGGCAACGCGTGGGGATCGGTCCTCGGCAACGCG
CTCGAGCACGGGATCGGTTACACGCCCTACGGCCTTCACGCCCGCAACCTCTGCGGCATCGAGGCGGTGCTGCCCGACGG
GGATCTGGTGCGCACCGGCATGGGTGCGATGAAGGACAACCCGTCGTGGCATCTCTTCCCGATGAGCTACGGGCCGACCT
GGGACCTCGCGTTCTCGCAATCGAACCTGGGCATCGTGACGAAGGCGGGCGTCTGGCTCCAGCCCGCGCCCGAAGCCTCG
CTAGAACTCGTCTGGGACATTCCCGAGGTCGATGACATCGCCTGGGTGGTCGATACGATCACCCCGCTCAAGATCTCGGG
CCTGATCGACCAGAACGTGTTCATCCCCTCGTGGCTCGGCAAGATGGTGCTCAAGGGGCAGCGCAAGGACTTCTGGGACA
AGCCCTCGGCCATTCCCGAATGGCGCGTGGCCGAACTGCTCAAGCAGTACAAGCTTGGCTACTGGCAGGTCGCGCTGCGC
CTCTATGGCGAGGAGACCGTGGTCAAGGCGCGGGCCGACGTGGTCAAGGCTGCGATGAAGCGCCATCTCGATGCGGCGCC
AGCGGAACACTGGTGGCGCCAGGGCGACCCGATCGGCCAGTACGAGACGACGATGGGCGTGCCTTCGGCAGTGCCGTTGC
AGATGTCCGACTGGATCGGCGGGCGCGGCGCGCACATGGGCTTCTCGCCCGTGGTTCCGGCCACCGGCAAGCACGTGCTG
GACCAGATGCACAGGAGCCGCAAGATCATCGCGGATTTCGACGTCGATTTCTACGCCAGCTTCACCATCGGCGGGCGCTT
TGCCAACAACGTCAACATGTTGATGTACGACCGCGACAATGCCGACGAAGTGGCCAAGATGCGCAAGCTGTTCGATGCTC
TCATCGTGGAGTCCGCGAAGGCGGGCTATGCCGAATACCGCACCCACCTCGGCTGGATGGACCCGGTGATGGAAACCTTC
GACTTCAACGGCCATGCGCTGCGCCGGTTCGACGAGAAGGTGAAGTCCGCGCTCGACCCCAACGGCATCATCGCACCCGG
CAAGCAGGGCGTCTGGCCCGCCAACTACGCCGCGATGCGGGGCAAGGCCTACAAGGGAGGGCAGTGA

Upstream 100 bases:

>100_bases
TGCTCGCAATGACGGAGCGATTGCTGCCACTTGACGGCAATTCGCAAGTGCTGCAAACAATAACAATTGTTAGAAAAAGA
TCGACGGGAGAAACCTACCG

Downstream 100 bases:

>100_bases
AATGAAGCGTATCCTGTTCGCCGCCACCCTCGTCGCCATCGGTGCCGTGGCCTATGCCGAACCGCCGGCGGGCGGGCCTC
CGCCGATGCCGCCCTATATA

Product: FAD linked oxidase-like protein

Products: NA

Alternate protein names: P-cresol methylhydroxylase; PCMH [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKLHLPPRVSPKQFQSAMTAFAGVVGKAWVMDSDADRDAYADTYAPGSTEEWPASAAVAPANVEEVRAIVRLANEHKVPL
WPVARGKNLGYGTAAPRMEGTVVMDLGRMNKVLELDHKLAYCVVEPGVGFFDLYDQIQREKAPLWMSVPGNAWGSVLGNA
LEHGIGYTPYGLHARNLCGIEAVLPDGDLVRTGMGAMKDNPSWHLFPMSYGPTWDLAFSQSNLGIVTKAGVWLQPAPEAS
LELVWDIPEVDDIAWVVDTITPLKISGLIDQNVFIPSWLGKMVLKGQRKDFWDKPSAIPEWRVAELLKQYKLGYWQVALR
LYGEETVVKARADVVKAAMKRHLDAAPAEHWWRQGDPIGQYETTMGVPSAVPLQMSDWIGGRGAHMGFSPVVPATGKHVL
DQMHRSRKIIADFDVDFYASFTIGGRFANNVNMLMYDRDNADEVAKMRKLFDALIVESAKAGYAEYRTHLGWMDPVMETF
DFNGHALRRFDEKVKSALDPNGIIAPGKQGVWPANYAAMRGKAYKGGQ

Sequences:

>Translated_528_residues
MKLHLPPRVSPKQFQSAMTAFAGVVGKAWVMDSDADRDAYADTYAPGSTEEWPASAAVAPANVEEVRAIVRLANEHKVPL
WPVARGKNLGYGTAAPRMEGTVVMDLGRMNKVLELDHKLAYCVVEPGVGFFDLYDQIQREKAPLWMSVPGNAWGSVLGNA
LEHGIGYTPYGLHARNLCGIEAVLPDGDLVRTGMGAMKDNPSWHLFPMSYGPTWDLAFSQSNLGIVTKAGVWLQPAPEAS
LELVWDIPEVDDIAWVVDTITPLKISGLIDQNVFIPSWLGKMVLKGQRKDFWDKPSAIPEWRVAELLKQYKLGYWQVALR
LYGEETVVKARADVVKAAMKRHLDAAPAEHWWRQGDPIGQYETTMGVPSAVPLQMSDWIGGRGAHMGFSPVVPATGKHVL
DQMHRSRKIIADFDVDFYASFTIGGRFANNVNMLMYDRDNADEVAKMRKLFDALIVESAKAGYAEYRTHLGWMDPVMETF
DFNGHALRRFDEKVKSALDPNGIIAPGKQGVWPANYAAMRGKAYKGGQ
>Mature_528_residues
MKLHLPPRVSPKQFQSAMTAFAGVVGKAWVMDSDADRDAYADTYAPGSTEEWPASAAVAPANVEEVRAIVRLANEHKVPL
WPVARGKNLGYGTAAPRMEGTVVMDLGRMNKVLELDHKLAYCVVEPGVGFFDLYDQIQREKAPLWMSVPGNAWGSVLGNA
LEHGIGYTPYGLHARNLCGIEAVLPDGDLVRTGMGAMKDNPSWHLFPMSYGPTWDLAFSQSNLGIVTKAGVWLQPAPEAS
LELVWDIPEVDDIAWVVDTITPLKISGLIDQNVFIPSWLGKMVLKGQRKDFWDKPSAIPEWRVAELLKQYKLGYWQVALR
LYGEETVVKARADVVKAAMKRHLDAAPAEHWWRQGDPIGQYETTMGVPSAVPLQMSDWIGGRGAHMGFSPVVPATGKHVL
DQMHRSRKIIADFDVDFYASFTIGGRFANNVNMLMYDRDNADEVAKMRKLFDALIVESAKAGYAEYRTHLGWMDPVMETF
DFNGHALRRFDEKVKSALDPNGIIAPGKQGVWPANYAAMRGKAYKGGQ

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=261, Percent_Identity=28.735632183908, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI37595754, Length=277, Percent_Identity=27.7978339350181, Blast_Score=80, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320027, Length=249, Percent_Identity=27.3092369477912, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR006094
- InterPro:   IPR016170
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: =1.17.99.1 [H]

Molecular weight: Translated: 58514; Mature: 58514

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHLPPRVSPKQFQSAMTAFAGVVGKAWVMDSDADRDAYADTYAPGSTEEWPASAAVAP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ANVEEVRAIVRLANEHKVPLWPVARGKNLGYGTAAPRMEGTVVMDLGRMNKVLELDHKLA
CCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCEE
YCVVEPGVGFFDLYDQIQREKAPLWMSVPGNAWGSVLGNALEHGIGYTPYGLHARNLCGI
EEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
EAVLPDGDLVRTGMGAMKDNPSWHLFPMSYGPTWDLAFSQSNLGIVTKAGVWLQPAPEAS
EEECCCCCCHHHCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCEEEECCEEECCCCCCC
LELVWDIPEVDDIAWVVDTITPLKISGLIDQNVFIPSWLGKMVLKGQRKDFWDKPSAIPE
EEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
WRVAELLKQYKLGYWQVALRLYGEETVVKARADVVKAAMKRHLDAAPAEHWWRQGDPIGQ
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
YETTMGVPSAVPLQMSDWIGGRGAHMGFSPVVPATGKHVLDQMHRSRKIIADFDVDFYAS
CHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEE
FTIGGRFANNVNMLMYDRDNADEVAKMRKLFDALIVESAKAGYAEYRTHLGWMDPVMETF
EEECCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHH
DFNGHALRRFDEKVKSALDPNGIIAPGKQGVWPANYAAMRGKAYKGGQ
CCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MKLHLPPRVSPKQFQSAMTAFAGVVGKAWVMDSDADRDAYADTYAPGSTEEWPASAAVAP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ANVEEVRAIVRLANEHKVPLWPVARGKNLGYGTAAPRMEGTVVMDLGRMNKVLELDHKLA
CCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCEE
YCVVEPGVGFFDLYDQIQREKAPLWMSVPGNAWGSVLGNALEHGIGYTPYGLHARNLCGI
EEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
EAVLPDGDLVRTGMGAMKDNPSWHLFPMSYGPTWDLAFSQSNLGIVTKAGVWLQPAPEAS
EEECCCCCCHHHCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCEEEECCEEECCCCCCC
LELVWDIPEVDDIAWVVDTITPLKISGLIDQNVFIPSWLGKMVLKGQRKDFWDKPSAIPE
EEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
WRVAELLKQYKLGYWQVALRLYGEETVVKARADVVKAAMKRHLDAAPAEHWWRQGDPIGQ
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
YETTMGVPSAVPLQMSDWIGGRGAHMGFSPVVPATGKHVLDQMHRSRKIIADFDVDFYAS
CHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEE
FTIGGRFANNVNMLMYDRDNADEVAKMRKLFDALIVESAKAGYAEYRTHLGWMDPVMETF
EEECCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHH
DFNGHALRRFDEKVKSALDPNGIIAPGKQGVWPANYAAMRGKAYKGGQ
CCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929007; 10565539; 3790500; 1846290; 10623531 [H]