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The map label for this gene is fixJ [H]

Identifier: 87200784

GI number: 87200784

Start: 2990334

End: 2990954

Strand: Reverse

Name: fixJ [H]

Synonym: Saro_2771

Alternate gene names: 87200784

Gene position: 2990954-2990334 (Counterclockwise)

Preceding gene: 87200785

Following gene: 87200781

Centisome position: 83.98

GC content: 67.47

Gene sequence:

>621_bases
ATGGTTGATCGCGTAACACTTCACATCCTCGATGGTGACACTGCCCGCCGGGCACAGCTCGCCCGCCTTGCATTCGCGGC
GGGTCACCACGCCGAAATCTACGCAAATGCGGAGGAGCTTCTGGCGCATGCGCCGTCTGCTGGCCTCGTCATCGCGCATG
ACGAACCGGTGGGTGAAGGCATCGCCGCGCTGATCTCGGAAATGATGCGTGCCGGGCACTGGCTTCCGGTCATCGCCGTT
GCCGATGCCCCTGAAACGGCGGCGGTCGTCCGGGGGATCAAGGCAGGCGCTCTGGATTACCTCGCGATGCCCACGCAGAT
CGCGCCCCTGAACGAAGCCGTGGCCCGTGCCGCCAAGGAAGCCGACGCCTTCCGGGTCCAGCGCGCCCGTGCCGCCGAAG
CGCGCCAGCGCATTGCACGCCTGTCCCTGCGGGAACGCGAAGTGCTTGATCGCCTGGCCGAAGGCTGCAGCAACAAGGCC
ATCGCCCGCGACCTGGATATCAGCCCGCGCACGGTCGAAATCCACCGGATGAAGATGATGGGCAAGCTGGGCGCGCGCCA
TGCGGCCGAAGCGGTCCGCCTGCGCATCGAAGCGGCCGGGCTCGACGACCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGAAAAACGTGTACGTAGCCCTTCTGAGGTGTGACTTTCGGTTCATTACGAAACTAACGCCTGTGGCAATGTTGCCGGTT
GCGGTGATGTAGTCCGGTTC

Downstream 100 bases:

>100_bases
CAAGCCGTCCCGCCTCAGCGGCAGAACGGCCTGAGGCCTCCGGGTTCGAGGTGAAGGTGATCTCGGTGCGCCTCGTTGTA
ATCGGGCGTCAACACAGTGC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MVDRVTLHILDGDTARRAQLARLAFAAGHHAEIYANAEELLAHAPSAGLVIAHDEPVGEGIAALISEMMRAGHWLPVIAV
ADAPETAAVVRGIKAGALDYLAMPTQIAPLNEAVARAAKEADAFRVQRARAAEARQRIARLSLREREVLDRLAEGCSNKA
IARDLDISPRTVEIHRMKMMGKLGARHAAEAVRLRIEAAGLDDLAA

Sequences:

>Translated_206_residues
MVDRVTLHILDGDTARRAQLARLAFAAGHHAEIYANAEELLAHAPSAGLVIAHDEPVGEGIAALISEMMRAGHWLPVIAV
ADAPETAAVVRGIKAGALDYLAMPTQIAPLNEAVARAAKEADAFRVQRARAAEARQRIARLSLREREVLDRLAEGCSNKA
IARDLDISPRTVEIHRMKMMGKLGARHAAEAVRLRIEAAGLDDLAA
>Mature_206_residues
MVDRVTLHILDGDTARRAQLARLAFAAGHHAEIYANAEELLAHAPSAGLVIAHDEPVGEGIAALISEMMRAGHWLPVIAV
ADAPETAAVVRGIKAGALDYLAMPTQIAPLNEAVARAAKEADAFRVQRARAAEARQRIARLSLREREVLDRLAEGCSNKA
IARDLDISPRTVEIHRMKMMGKLGARHAAEAVRLRIEAAGLDDLAA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22105; Mature: 22105

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDRVTLHILDGDTARRAQLARLAFAAGHHAEIYANAEELLAHAPSAGLVIAHDEPVGEG
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHCCCCCEEEECCCCCCHH
IAALISEMMRAGHWLPVIAVADAPETAAVVRGIKAGALDYLAMPTQIAPLNEAVARAAKE
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
ADAFRVQRARAAEARQRIARLSLREREVLDRLAEGCSNKAIARDLDISPRTVEIHRMKMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHH
GKLGARHAAEAVRLRIEAAGLDDLAA
HHHHHHHHHHHHHHHHEECCCHHCCC
>Mature Secondary Structure
MVDRVTLHILDGDTARRAQLARLAFAAGHHAEIYANAEELLAHAPSAGLVIAHDEPVGEG
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHCCCCCEEEECCCCCCHH
IAALISEMMRAGHWLPVIAVADAPETAAVVRGIKAGALDYLAMPTQIAPLNEAVARAAKE
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
ADAFRVQRARAAEARQRIARLSLREREVLDRLAEGCSNKAIARDLDISPRTVEIHRMKMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHH
GKLGARHAAEAVRLRIEAAGLDDLAA
HHHHHHHHHHHHHHHHEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]