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The map label for this gene is dinB2 [H]

Identifier: 87200669

GI number: 87200669

Start: 2893230

End: 2894366

Strand: Reverse

Name: dinB2 [H]

Synonym: Saro_2656

Alternate gene names: 87200669

Gene position: 2894366-2893230 (Counterclockwise)

Preceding gene: 87200671

Following gene: 87200667

Centisome position: 81.27

GC content: 66.67

Gene sequence:

>1137_bases
ATGGACCCTCGATCGGCAGATGGCGAACCGGATCGCAGCGCGACCGGGCTGCGCAAGGTCATCCATGTCGACATGGACGC
CTTCTTCGCCAGCGTCGAGCAACGCGACAATCCGGACCTGCGCGGAAAGCCGGTCGCGGTCGGCGGTTCCTCCGGGCGCG
GGGTCGTTGCCGCGGCAAGTTACGAGGCCCGCAAGTTCGGCGTACGATCGGCTATGCCTTCGGCGAGGGCCATCGTGCTG
TGTCCCGACCTGATCTTCTGCAGGCCGCGATTCGATGTCTATCGCTCCGTTTCCCAGCAGATCCGCGCCATCTTCCTCGA
CTACACGCCCCACGTCGAACCCCTGTCGCTCGACGAGGCCTATCTCGACGTGACCGATGACGTGCGCGGCATCGGTTCCG
CCACGCGGATCGCCGAACTCATCCGCCGGCGGATCAAGGCCGACACCGGGCTGACCGCCAGTGCCGGTGTGTCCTACAAC
AAGTTCCTCGCCAAGATCGCGAGCGACCAGAACAAGCCCGACGGCATGTGCGTGATCCGGCCCGGCGAGGGCGCGCAGTT
CGTCGCCAGCCTTCCGGTGCGGCGCTTCCACGGCATCGGACCGCGCGGTGCGGAAAAGATGGCGGCGCTCGGGATAGAGA
CGGGCGCGGATCTGCGTGCCAGGGACCTGCCTTTCCTGCGCCAGCATTTCGGCAGCCTCGCGGACTATCTCTACCGGGCG
GTGCGGGGCATCGACCTGCGCCAGGTGAAGGCCGACAGGCCGCGCAAGTCGGTCGGCGCGGAGCGGACGTTCGAGCGTGA
CATTTCGTCCGGCCCGGCCTTGCGCGAAACGCTGGAGCGCATCCTGGAGATCGTGTGGGATCGGATCGAGCGCAGCGGGG
CCAGCGGTCGGACGGTCACCCTCAAGATGAAATTCAACGACTTCACCCCAATTACCCGTGCCCGCTCCCTGCCGCGCCCG
ATCGCAGACAAGGAGGAATTTGCCCGGCTGTCGCGTGAACTGCTCGATGCGCAACTGCCGCTTGCCAAGCCGATCAGGCT
GATGGGGCTGACGCTGTCCGCTCTCGAGGGCGAGGAGCCGGAAGAGGCCGAGGACGGTCCCTCCGGCGCAGCGCTTCAAG
CAGAACTGCCCTTCTGA

Upstream 100 bases:

>100_bases
GAGGTGTTTGAATTGTTAGCGTTCATAACTGCCATTTGAACTAGTTCGCACGCGAATCCCACCCTTTATGTTGCAATGCG
AAACGGGTTAGCATCGCGAA

Downstream 100 bases:

>100_bases
TGGTCGAGCCATCGCGCGATCATGGCGCGGGCTGCCTCACTGGTGCGCTCGGGCAGTGCGTCGGTGGGAAAGAACCGGGC
TTCCTCGATTTCACGCGCGT

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV 2 [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MDPRSADGEPDRSATGLRKVIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRGVVAAASYEARKFGVRSAMPSARAIVL
CPDLIFCRPRFDVYRSVSQQIRAIFLDYTPHVEPLSLDEAYLDVTDDVRGIGSATRIAELIRRRIKADTGLTASAGVSYN
KFLAKIASDQNKPDGMCVIRPGEGAQFVASLPVRRFHGIGPRGAEKMAALGIETGADLRARDLPFLRQHFGSLADYLYRA
VRGIDLRQVKADRPRKSVGAERTFERDISSGPALRETLERILEIVWDRIERSGASGRTVTLKMKFNDFTPITRARSLPRP
IADKEEFARLSRELLDAQLPLAKPIRLMGLTLSALEGEEPEEAEDGPSGAALQAELPF

Sequences:

>Translated_378_residues
MDPRSADGEPDRSATGLRKVIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRGVVAAASYEARKFGVRSAMPSARAIVL
CPDLIFCRPRFDVYRSVSQQIRAIFLDYTPHVEPLSLDEAYLDVTDDVRGIGSATRIAELIRRRIKADTGLTASAGVSYN
KFLAKIASDQNKPDGMCVIRPGEGAQFVASLPVRRFHGIGPRGAEKMAALGIETGADLRARDLPFLRQHFGSLADYLYRA
VRGIDLRQVKADRPRKSVGAERTFERDISSGPALRETLERILEIVWDRIERSGASGRTVTLKMKFNDFTPITRARSLPRP
IADKEEFARLSRELLDAQLPLAKPIRLMGLTLSALEGEEPEEAEDGPSGAALQAELPF
>Mature_378_residues
MDPRSADGEPDRSATGLRKVIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRGVVAAASYEARKFGVRSAMPSARAIVL
CPDLIFCRPRFDVYRSVSQQIRAIFLDYTPHVEPLSLDEAYLDVTDDVRGIGSATRIAELIRRRIKADTGLTASAGVSYN
KFLAKIASDQNKPDGMCVIRPGEGAQFVASLPVRRFHGIGPRGAEKMAALGIETGADLRARDLPFLRQHFGSLADYLYRA
VRGIDLRQVKADRPRKSVGAERTFERDISSGPALRETLERILEIVWDRIERSGASGRTVTLKMKFNDFTPITRARSLPRP
IADKEEFARLSRELLDAQLPLAKPIRLMGLTLSALEGEEPEEAEDGPSGAALQAELPF

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=338, Percent_Identity=30.4733727810651, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI7706681, Length=339, Percent_Identity=30.3834808259587, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI7705344, Length=129, Percent_Identity=47.2868217054264, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI154350220, Length=321, Percent_Identity=28.3489096573209, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI5729982, Length=146, Percent_Identity=40.4109589041096, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786425, Length=347, Percent_Identity=46.1095100864553, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1787432, Length=266, Percent_Identity=31.5789473684211, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193205700, Length=408, Percent_Identity=31.3725490196078, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17537959, Length=279, Percent_Identity=32.258064516129, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193205702, Length=285, Percent_Identity=31.9298245614035, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI115534089, Length=124, Percent_Identity=37.9032258064516, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19923006, Length=337, Percent_Identity=26.4094955489614, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355641, Length=337, Percent_Identity=28.486646884273, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24644984, Length=337, Percent_Identity=28.486646884273, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24668444, Length=249, Percent_Identity=26.9076305220884, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41515; Mature: 41515

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPRSADGEPDRSATGLRKVIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRGVVAAAS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEECC
YEARKFGVRSAMPSARAIVLCPDLIFCRPRFDVYRSVSQQIRAIFLDYTPHVEPLSLDEA
HHHHHHHHHHCCCCCCEEEECCCHHEECCHHHHHHHHHHHHHHEEECCCCCCCCCCCCHH
YLDVTDDVRGIGSATRIAELIRRRIKADTGLTASAGVSYNKFLAKIASDQNKPDGMCVIR
HHCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEE
PGEGAQFVASLPVRRFHGIGPRGAEKMAALGIETGADLRARDLPFLRQHFGSLADYLYRA
CCCCCHHHHHCCHHHHCCCCCCCHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
VRGIDLRQVKADRPRKSVGAERTFERDISSGPALRETLERILEIVWDRIERSGASGRTVT
HHCCCHHHHHCCCCHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LKMKFNDFTPITRARSLPRPIADKEEFARLSRELLDAQLPLAKPIRLMGLTLSALEGEEP
EEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
EEAEDGPSGAALQAELPF
CCCCCCCCCCEEEECCCC
>Mature Secondary Structure
MDPRSADGEPDRSATGLRKVIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRGVVAAAS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEECC
YEARKFGVRSAMPSARAIVLCPDLIFCRPRFDVYRSVSQQIRAIFLDYTPHVEPLSLDEA
HHHHHHHHHHCCCCCCEEEECCCHHEECCHHHHHHHHHHHHHHEEECCCCCCCCCCCCHH
YLDVTDDVRGIGSATRIAELIRRRIKADTGLTASAGVSYNKFLAKIASDQNKPDGMCVIR
HHCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEE
PGEGAQFVASLPVRRFHGIGPRGAEKMAALGIETGADLRARDLPFLRQHFGSLADYLYRA
CCCCCHHHHHCCHHHHCCCCCCCHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
VRGIDLRQVKADRPRKSVGAERTFERDISSGPALRETLERILEIVWDRIERSGASGRTVT
HHCCCHHHHHCCCCHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LKMKFNDFTPITRARSLPRPIADKEEFARLSRELLDAQLPLAKPIRLMGLTLSALEGEEP
EEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
EEAEDGPSGAALQAELPF
CCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]