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The map label for this gene is puuE [H]

Identifier: 87200615

GI number: 87200615

Start: 2812382

End: 2813683

Strand: Reverse

Name: puuE [H]

Synonym: Saro_2602

Alternate gene names: 87200615

Gene position: 2813683-2812382 (Counterclockwise)

Preceding gene: 87200616

Following gene: 87200614

Centisome position: 79.0

GC content: 67.36

Gene sequence:

>1302_bases
ATGACCACGAACGCCGAACTCTGGACCCGTCGCGAAGCCGCCGTGCCGCGCGGTGTCTCCTCGATGCACCAGCGCTTCTT
CGCGCGGGGTGAAAATGCCGAGGCCTTCGATGCTGAAGGCAACCGCTACATCGATTTCGCCACCGGCATCGCGGTGTGCA
ACACCGGCCACGGGCATCCGCGCGTGCTTGCGGCGGTGCAGCGCCAGCTTGACGCGTTTTCGCACTGCTCGTTCCAGGTG
ACGCCTTACGAAAACTACATCGAGCTGGCCGAACGGCTGAATGCGATTGCTCCCATCGACGGACCGGTGAAGTCGATCTT
CTTCACCACCGGTGCCGAGGCGCTGGAGAACGCTGTGAAGATCGCGCGTGCGCATACCGGGCGCCGCGGCATCATCACTT
TCCAGGGCGGCTATCACGGTCGCACGCTGCTGACGCTGGCGATGACCGGCAAGGTCCTGCCCTACAAGGCGCGGTTCGGG
CCGATGCCGGGCGATGTGTTCCATGCGCGCTTTCCCATCCCCTACCACGGCTTTTCCGAGGAAGAGGCGATTGCCGGGAT
CGAGGCGCTGTTTGCCTCGGCCATCGAACCTTCGGCGGTTGCGGCGATCGTGCTGGAACCGGTCCTGGGCGAGGGCGGGT
TCTACACCGCGTCCTATGCCTTCTTCCGTGCCTTGCGGGAGATCTGCGACCGTCACGGCATTCTGCTGATCGCGGACGAG
GTCCAGGCCGGTTTTGCCCGCACTGGCCGCATGTTCGCGATGGACTGGGTGCGCGAGGCCACCGGCGTGACGCCGGACCT
GATGACCGTTGCCAAGGCGATGGCGGGGGGTTTCCCCATTTCGGGGGTGATGGGCCGGGCCGAGATCATGGACGCGCCCG
ATCCGGGCGGGCTTGGCGGCACCTATGGCGGATCGCCACTGGGCTGCGCGGCGGGGCTGGCGGTGCTCGACGTGATTGCC
GACGAGGACTTGTGCGCACGTGCCGTCGCCATCGGGAACCGGATCAAGGCCCGCCTGCGCCGGATGCGCGACAATGGCAT
GACCGCCATCGGCGACGTGCGCGGGCCGGGCGCGATGATTGCGATGGAACTGGTCCACGATGGCGATCCGTCGCGGCCGA
ACCCGGATGCGACCCGTGCGCTGGTGCAGGAAGGCGCGCGCGAGGGCCTGTTGCTGCTGTCGTGCGGGCTGCGCGGGAAC
GTCGTGCGCTTCCTGCCGGCGCTGACCATGCCGGACGCCCTGCTCGACGAAGCGCTCGACAGGCTGGAGATTGCGCTGAA
CCGGGTGCTGGGCGGGCAGTGA

Upstream 100 bases:

>100_bases
TCGGCGGGATCAAGCATTCGGGCCTGGGCCGCGAGGGATCGCGCCATGGCCTCGATGAATACCTCGAACTCAAATACTGC
CTGATGGGAGGCATCGACCG

Downstream 100 bases:

>100_bases
GCGCCTGAATGGCCAGATAAGGCAAGCGGCGTGGTGGGGGCAGGCGCAACCTTCACGCCACAACAATAGCAATCCGGCCT
CGTGTCTGCCCCCGCGGATG

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTTNAELWTRREAAVPRGVSSMHQRFFARGENAEAFDAEGNRYIDFATGIAVCNTGHGHPRVLAAVQRQLDAFSHCSFQV
TPYENYIELAERLNAIAPIDGPVKSIFFTTGAEALENAVKIARAHTGRRGIITFQGGYHGRTLLTLAMTGKVLPYKARFG
PMPGDVFHARFPIPYHGFSEEEAIAGIEALFASAIEPSAVAAIVLEPVLGEGGFYTASYAFFRALREICDRHGILLIADE
VQAGFARTGRMFAMDWVREATGVTPDLMTVAKAMAGGFPISGVMGRAEIMDAPDPGGLGGTYGGSPLGCAAGLAVLDVIA
DEDLCARAVAIGNRIKARLRRMRDNGMTAIGDVRGPGAMIAMELVHDGDPSRPNPDATRALVQEGAREGLLLLSCGLRGN
VVRFLPALTMPDALLDEALDRLEIALNRVLGGQ

Sequences:

>Translated_433_residues
MTTNAELWTRREAAVPRGVSSMHQRFFARGENAEAFDAEGNRYIDFATGIAVCNTGHGHPRVLAAVQRQLDAFSHCSFQV
TPYENYIELAERLNAIAPIDGPVKSIFFTTGAEALENAVKIARAHTGRRGIITFQGGYHGRTLLTLAMTGKVLPYKARFG
PMPGDVFHARFPIPYHGFSEEEAIAGIEALFASAIEPSAVAAIVLEPVLGEGGFYTASYAFFRALREICDRHGILLIADE
VQAGFARTGRMFAMDWVREATGVTPDLMTVAKAMAGGFPISGVMGRAEIMDAPDPGGLGGTYGGSPLGCAAGLAVLDVIA
DEDLCARAVAIGNRIKARLRRMRDNGMTAIGDVRGPGAMIAMELVHDGDPSRPNPDATRALVQEGAREGLLLLSCGLRGN
VVRFLPALTMPDALLDEALDRLEIALNRVLGGQ
>Mature_432_residues
TTNAELWTRREAAVPRGVSSMHQRFFARGENAEAFDAEGNRYIDFATGIAVCNTGHGHPRVLAAVQRQLDAFSHCSFQVT
PYENYIELAERLNAIAPIDGPVKSIFFTTGAEALENAVKIARAHTGRRGIITFQGGYHGRTLLTLAMTGKVLPYKARFGP
MPGDVFHARFPIPYHGFSEEEAIAGIEALFASAIEPSAVAAIVLEPVLGEGGFYTASYAFFRALREICDRHGILLIADEV
QAGFARTGRMFAMDWVREATGVTPDLMTVAKAMAGGFPISGVMGRAEIMDAPDPGGLGGTYGGSPLGCAAGLAVLDVIAD
EDLCARAVAIGNRIKARLRRMRDNGMTAIGDVRGPGAMIAMELVHDGDPSRPNPDATRALVQEGAREGLLLLSCGLRGNV
VRFLPALTMPDALLDEALDRLEIALNRVLGGQ

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=429, Percent_Identity=32.8671328671329, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI4557809, Length=419, Percent_Identity=31.2649164677804, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI37574042, Length=459, Percent_Identity=29.8474945533769, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI226442705, Length=459, Percent_Identity=29.4117647058824, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI226442709, Length=396, Percent_Identity=30.8080808080808, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI24119277, Length=419, Percent_Identity=27.4463007159904, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI284507298, Length=334, Percent_Identity=32.6347305389222, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI188536080, Length=437, Percent_Identity=27.2311212814645, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI38679950, Length=437, Percent_Identity=27.2311212814645, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI38679946, Length=437, Percent_Identity=27.2311212814645, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1787560, Length=425, Percent_Identity=54.1176470588235, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1789016, Length=420, Percent_Identity=53.3333333333333, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789759, Length=411, Percent_Identity=35.5231143552311, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1788044, Length=404, Percent_Identity=36.1386138613861, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI145693181, Length=343, Percent_Identity=33.5276967930029, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786991, Length=414, Percent_Identity=29.2270531400966, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1786349, Length=338, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32564660, Length=445, Percent_Identity=30.3370786516854, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25144271, Length=409, Percent_Identity=27.3838630806846, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71992977, Length=434, Percent_Identity=28.110599078341, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25144274, Length=296, Percent_Identity=27.027027027027, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17541228, Length=464, Percent_Identity=25.4310344827586, Blast_Score=111, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=413, Percent_Identity=30.0242130750605, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323470, Length=412, Percent_Identity=29.126213592233, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6324386, Length=418, Percent_Identity=22.9665071770335, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321456, Length=312, Percent_Identity=23.7179487179487, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28574759, Length=456, Percent_Identity=30.9210526315789, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI161085790, Length=453, Percent_Identity=30.9050772626932, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21357415, Length=409, Percent_Identity=29.8288508557457, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21356575, Length=409, Percent_Identity=31.5403422982885, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI281366494, Length=441, Percent_Identity=24.9433106575964, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24667139, Length=441, Percent_Identity=24.9433106575964, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24667143, Length=441, Percent_Identity=24.9433106575964, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 46311; Mature: 46180

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNAELWTRREAAVPRGVSSMHQRFFARGENAEAFDAEGNRYIDFATGIAVCNTGHGHP
CCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEECCCCCEEEEEECCEEEECCCCCCH
RVLAAVQRQLDAFSHCSFQVTPYENYIELAERLNAIAPIDGPVKSIFFTTGAEALENAVK
HHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCHHHHEECCCHHHHHHHHH
IARAHTGRRGIITFQGGYHGRTLLTLAMTGKVLPYKARFGPMPGDVFHARFPIPYHGFSE
HHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCEEHHCCCCCCCCCEEEECCCCCCCCCC
EEAIAGIEALFASAIEPSAVAAIVLEPVLGEGGFYTASYAFFRALREICDRHGILLIADE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECC
VQAGFARTGRMFAMDWVREATGVTPDLMTVAKAMAGGFPISGVMGRAEIMDAPDPGGLGG
HHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCHHHCCCCCCCCCCCC
TYGGSPLGCAAGLAVLDVIADEDLCARAVAIGNRIKARLRRMRDNGMTAIGDVRGPGAMI
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHE
AMELVHDGDPSRPNPDATRALVQEGAREGLLLLSCGLRGNVVRFLPALTMPDALLDEALD
EEEEHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHH
RLEIALNRVLGGQ
HHHHHHHHHHCCC
>Mature Secondary Structure 
TTNAELWTRREAAVPRGVSSMHQRFFARGENAEAFDAEGNRYIDFATGIAVCNTGHGHP
CCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEECCCCCEEEEEECCEEEECCCCCCH
RVLAAVQRQLDAFSHCSFQVTPYENYIELAERLNAIAPIDGPVKSIFFTTGAEALENAVK
HHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCHHHHEECCCHHHHHHHHH
IARAHTGRRGIITFQGGYHGRTLLTLAMTGKVLPYKARFGPMPGDVFHARFPIPYHGFSE
HHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCEEHHCCCCCCCCCEEEECCCCCCCCCC
EEAIAGIEALFASAIEPSAVAAIVLEPVLGEGGFYTASYAFFRALREICDRHGILLIADE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECC
VQAGFARTGRMFAMDWVREATGVTPDLMTVAKAMAGGFPISGVMGRAEIMDAPDPGGLGG
HHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCHHHCCCCCCCCCCCC
TYGGSPLGCAAGLAVLDVIADEDLCARAVAIGNRIKARLRRMRDNGMTAIGDVRGPGAMI
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHE
AMELVHDGDPSRPNPDATRALVQEGAREGLLLLSCGLRGNVVRFLPALTMPDALLDEALD
EEEEHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHH
RLEIALNRVLGGQ
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]