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The map label for this gene is queF

Identifier: 87200564

GI number: 87200564

Start: 2753941

End: 2754441

Strand: Reverse

Name: queF

Synonym: Saro_2551

Alternate gene names: 87200564

Gene position: 2754441-2753941 (Counterclockwise)

Preceding gene: 87200580

Following gene: 87200562

Centisome position: 77.34

GC content: 61.48

Gene sequence:

>501_bases
GTGCCGTCAGGTGCTTTTAGGTGCCGTTCAGGTCACAAGGCGGTCGTCATGGCAAATACACCACTTTTCCTCGGACAAAA
CAGCAGCTTGCCAGCTTCTCCGGACGAGGCTGTGCTCGATTACGTGGCCAATCCGCGTCCCGGCGCGCTTTACATGATCC
GCTTTGCTGCGCCCGAATTCACCTCGCTCTGCCCGGTCACCGGCCAGCCCGACTTCGCACACCTCGTGATCGATTATGCG
CCCGGTGAATGCATCGTGGAATCAAAGGCCTTGAAGCTGTTCCTCGGATCGTTTCGCAACCATGCCGGCTTCCACGAGGA
CGTGACCGTCGGCATCGGCCAGCGCCTGTTCGACGAGATGAAGCCGCAATGGCTGCGCATCGGTGGCTACTGGTACCCGC
GCGGCGGAATTCCGATTGACGTGTTCTGGCAATCGGGTCCGCCGCCGGCCGGTCTTTGGCTTCCGGATCAAGGGGTTGCG
CCTTACCGCGGACGCGGCTGA

Upstream 100 bases:

>100_bases
GATGAACCGATGGGCATGATCGGCTCACTTGCCCTGCGGCTGCGACCGGTGCATTGGGCGCGCAACAGAAGGGCTTCGGG
GTGCGCCAAGGTGCATTTCG

Downstream 100 bases:

>100_bases
CGCATGGCGCGCCGCAGCCCGCTCAGGCAGCGAGCTGCGGCAACCGGAAGGTCTCGCGCTGTTCCGGCGCAAGCATGACG
ACAAGCGCCTTGACCACGCG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MPSGAFRCRSGHKAVVMANTPLFLGQNSSLPASPDEAVLDYVANPRPGALYMIRFAAPEFTSLCPVTGQPDFAHLVIDYA
PGECIVESKALKLFLGSFRNHAGFHEDVTVGIGQRLFDEMKPQWLRIGGYWYPRGGIPIDVFWQSGPPPAGLWLPDQGVA
PYRGRG

Sequences:

>Translated_166_residues
MPSGAFRCRSGHKAVVMANTPLFLGQNSSLPASPDEAVLDYVANPRPGALYMIRFAAPEFTSLCPVTGQPDFAHLVIDYA
PGECIVESKALKLFLGSFRNHAGFHEDVTVGIGQRLFDEMKPQWLRIGGYWYPRGGIPIDVFWQSGPPPAGLWLPDQGVA
PYRGRG
>Mature_165_residues
PSGAFRCRSGHKAVVMANTPLFLGQNSSLPASPDEAVLDYVANPRPGALYMIRFAAPEFTSLCPVTGQPDFAHLVIDYAP
GECIVESKALKLFLGSFRNHAGFHEDVTVGIGQRLFDEMKPQWLRIGGYWYPRGGIPIDVFWQSGPPPAGLWLPDQGVAP
YRGRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_NOVAD (Q2G586)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497821.1
- ProteinModelPortal:   Q2G586
- SMR:   Q2G586
- STRING:   Q2G586
- GeneID:   3916873
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2551
- NMPDR:   fig|48935.1.peg.3156
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   HEDCTVA
- ProtClustDB:   PRK13258
- BioCyc:   NARO279238:SARO_2551-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 18145; Mature: 18014

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00196 COPPER_BLUE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSGAFRCRSGHKAVVMANTPLFLGQNSSLPASPDEAVLDYVANPRPGALYMIRFAAPEF
CCCCCCEECCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCC
TSLCPVTGQPDFAHLVIDYAPGECIVESKALKLFLGSFRNHAGFHEDVTVGIGQRLFDEM
CCCCCCCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHC
KPQWLRIGGYWYPRGGIPIDVFWQSGPPPAGLWLPDQGVAPYRGRG
CCCEEEECCEEECCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure 
PSGAFRCRSGHKAVVMANTPLFLGQNSSLPASPDEAVLDYVANPRPGALYMIRFAAPEF
CCCCCEECCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCC
TSLCPVTGQPDFAHLVIDYAPGECIVESKALKLFLGSFRNHAGFHEDVTVGIGQRLFDEM
CCCCCCCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHC
KPQWLRIGGYWYPRGGIPIDVFWQSGPPPAGLWLPDQGVAPYRGRG
CCCEEEECCEEECCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA