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The map label for this gene is phoB [H]

Identifier: 87200450

GI number: 87200450

Start: 2620391

End: 2621065

Strand: Direct

Name: phoB [H]

Synonym: Saro_2437

Alternate gene names: 87200450

Gene position: 2620391-2621065 (Clockwise)

Preceding gene: 87200449

Following gene: 87200453

Centisome position: 73.57

GC content: 68.3

Gene sequence:

>675_bases
ATGCGGCGTATCGATATCTTGCTGACCGGGGATCTGCCGGGGGGCCGCGAGCCCTTCGTGCATTCTGACTTGCGCGTCGT
GTTCCATCGCTGGAAATCCGACGCGCCGCTGCCGCTCATGGAAGGGCGGCCCTGGGCTTTCATCGACTGGGTGCTTCCGG
ACCTGTCCGGTCTCGAACTCTGCCGCCGCCTGCGCTGCGCCGACGCGCTGGGCCAGGACGCGCACATCACCATGATCCTC
GAGGAAAACGACGCCGAAGCCAAGCGCCGCGCGCTCAAGGCCGGGGCCGACGACTACATCCTCGCGCCGGTCGATCGCAA
CGCGCTGCTCGACCGTGTCCTCTCGGTCCAGTTGCCGGAACGCGAGACGCCTGCTCGCCAGCTCCGCCTCGGCGAACTTA
CGCTCGACCTTGCCGCGCTGCAGGCACGGTGGCGTGAAAAGCCGCTGATGCTCATGCCCAACGAGCTGCGCCTGCTGCGC
TACCTGATGGAGCAACCGGGCCACGTCTTCTCGCGCCAGCAACTCATCACCGCGCTGGGCAAGCACGACCAGCCGATCGA
CGAGCGCACTGTCGATGCCTGGGTCAGCCGCCTGCGCCGCGCCCTGCGCGATGCTGGAGCAGGTTCGCCGCTGCGCACGG
TGCGCTCGCTGGGCTACGTGCTCGACCGCCCCTGA

Upstream 100 bases:

>100_bases
AAAACTACGTCGGCCTGCCGGTCTGACAAGGTTGTAACATCGTCATGTTCCCGTCACACGGGCGGGACAGAAAGCCCCTG
TTCGACAGGGGCTTTCTTGC

Downstream 100 bases:

>100_bases
CACCTTCTCCTCCCCGGCGCTGCTCCCTGGATTGGCAGCGTCAAACCCCGCCCGGCGTTCATTCACCGGGCGGGGTTTTC
CATTCGGGCAGTGCGCTACT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MRRIDILLTGDLPGGREPFVHSDLRVVFHRWKSDAPLPLMEGRPWAFIDWVLPDLSGLELCRRLRCADALGQDAHITMIL
EENDAEAKRRALKAGADDYILAPVDRNALLDRVLSVQLPERETPARQLRLGELTLDLAALQARWREKPLMLMPNELRLLR
YLMEQPGHVFSRQQLITALGKHDQPIDERTVDAWVSRLRRALRDAGAGSPLRTVRSLGYVLDRP

Sequences:

>Translated_224_residues
MRRIDILLTGDLPGGREPFVHSDLRVVFHRWKSDAPLPLMEGRPWAFIDWVLPDLSGLELCRRLRCADALGQDAHITMIL
EENDAEAKRRALKAGADDYILAPVDRNALLDRVLSVQLPERETPARQLRLGELTLDLAALQARWREKPLMLMPNELRLLR
YLMEQPGHVFSRQQLITALGKHDQPIDERTVDAWVSRLRRALRDAGAGSPLRTVRSLGYVLDRP
>Mature_224_residues
MRRIDILLTGDLPGGREPFVHSDLRVVFHRWKSDAPLPLMEGRPWAFIDWVLPDLSGLELCRRLRCADALGQDAHITMIL
EENDAEAKRRALKAGADDYILAPVDRNALLDRVLSVQLPERETPARQLRLGELTLDLAALQARWREKPLMLMPNELRLLR
YLMEQPGHVFSRQQLITALGKHDQPIDERTVDAWVSRLRRALRDAGAGSPLRTVRSLGYVLDRP

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=184, Percent_Identity=31.5217391304348, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1790860, Length=178, Percent_Identity=35.3932584269663, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788394, Length=193, Percent_Identity=34.7150259067358, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789809, Length=184, Percent_Identity=29.3478260869565, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1789402, Length=177, Percent_Identity=31.0734463276836, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786784, Length=181, Percent_Identity=26.5193370165746, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25600; Mature: 25600

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIDILLTGDLPGGREPFVHSDLRVVFHRWKSDAPLPLMEGRPWAFIDWVLPDLSGLEL
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHH
CRRLRCADALGQDAHITMILEENDAEAKRRALKAGADDYILAPVDRNALLDRVLSVQLPE
HHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCC
RETPARQLRLGELTLDLAALQARWREKPLMLMPNELRLLRYLMEQPGHVFSRQQLITALG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHC
KHDQPIDERTVDAWVSRLRRALRDAGAGSPLRTVRSLGYVLDRP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRIDILLTGDLPGGREPFVHSDLRVVFHRWKSDAPLPLMEGRPWAFIDWVLPDLSGLEL
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHH
CRRLRCADALGQDAHITMILEENDAEAKRRALKAGADDYILAPVDRNALLDRVLSVQLPE
HHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCC
RETPARQLRLGELTLDLAALQARWREKPLMLMPNELRLLRYLMEQPGHVFSRQQLITALG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHC
KHDQPIDERTVDAWVSRLRRALRDAGAGSPLRTVRSLGYVLDRP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]