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The map label for this gene is recJ [H]

Identifier: 87200288

GI number: 87200288

Start: 2413476

End: 2415197

Strand: Direct

Name: recJ [H]

Synonym: Saro_2274

Alternate gene names: 87200288

Gene position: 2413476-2415197 (Clockwise)

Preceding gene: 87200283

Following gene: 87200289

Centisome position: 67.76

GC content: 70.91

Gene sequence:

>1722_bases
ATGGACCTCGGCGGCGCGGGTGGCATCCAGGGTGGCGGGCTTTCCGACGACATCGTGACGCAGCTCCTGCTGTCGCGCGG
AGTGGGCCGCGAGGACCTCGACCGCCATCGCCGCCCGACGTTGCGCGACTTCCTGCCGGACCCGTCGATCTTCCAGGACA
TGGACAACGCCGCACGCCGGCTGACCGATGCGATCCTGTCGGGCGAGCGCATCACGATCTACGGCGACTACGACGTCGAT
GGGGCGACCAGCGCGGCGCTGCTGATCCGGCTGCTGCGCGAACTTGGCGTCGATGCGGGGCACTATATCCCCGACCGCCT
GCTGGAAGGCTATGGGCCCTCGGGCGAAGCCCTGGTCCGCCTGGCGCGCGAGGGGTCGAATCTCATCGTCACGGTCGACT
GCGGCGCGATGGCGCACGAAGCGCTTGCGGCGGCGGCAGCCGAAGGCGTGGACGTAATCGTCGTCGACCACCACAAGTGC
GCCCCCGAGCTTCCCCGCGCGGCGGCGCTGGTCAATCCCAACCGCCTTGACGAATGCGACGAAGCTGCCGCCCACGGGCA
TCTGGCAGCCGTGGGCGTCGCCTTCCTGCTGGCGGTGGCGACCGTGCGCGAACTGCGCGGACGCGGCTTTTTCGACGGGC
GCAAGGCACCGGACCTGATGGCACTGCTGGACCTCGTGGCGCTGGGCACGGTGGCCGACGTCGCGCAGCTCAAGGGTCTC
AACCGCGCGCTCGTCTCGCAGGGGCTCAAGATCATGGCGCGGCGCGAGAACGTCGGCCTTTCCGCGCTGATCGACGCCAG
CCGGCTGAGCCGCGCGCCCACGTGCAGCGATCTCGGCTTCGCGCTCGGGCCGCGGATCAATGCGGGCGGGCGCGTTGGCG
AGGCGACGCTGGGCGTACGCCTGCTGACGACCGAGGACCCGGAGGAAGCGCGGGCGATCTCGGCCCAGCTATCGCGGCTC
AACGACGAGCGCCGCGCCATCGAGCAGGCCGTGCAGGAGGCCGCCGAGGCGCAGGTCGACGCGCAGCACAACCGGGCCGT
GATGGTCCTGGCGGGCAGCGGCTGGCATCCGGGCGTGATCGGCATCGTGGCCGGACGGATCAAGGAGAAGACCGGCAAGC
CGACCCTCGTGATCGCGCTCGACGCGGACGAGGCCGGGCATGGCAAGGGGTCGGGCCGCTCGATCTCGGGGGTGGACCTT
GGCGCGGCGATCATCGCCGCGCGCGAGGCCGGATTGCTGGTGGCGGGCGGCGGACACGCCATGGCCTGCGGATTGACCAT
CGAGCCTTCAGCGCTGTCGCGACTTGCGGATTGGCTGGACGAAAGGCTTTCACGCGACGTGACGGCGGCGCAATCGTCGC
AGGCGCTGCTGCTGGACCTTTCGCTAAGTCCGGGCGGGCTGACGCCGGATCTGGTCGAGACGCTCGAGGCGGCAGGCCCG
TTCGGCGTGGGCTGGCCCGGGCCGCGCGTGGCAGTGGGCCCGGTGCGGCTGGTCAAGTGCGACCTCGTCGGCACCGACCA
CGTGAGGATGGTCGCCGCAGGTGCCGACGGCCGGTCGTTCAAGGCCATCGCCTTTCGCGCGGCGCAGAGCGAACTTGGAC
AGGTTCTGCTCAACGCATCGCGCGGCCGACAGTTCTGGCTGGCGGGTCGCGCGCGGATCGATGACTGGGCCAGCCGCCCG
GCGGCCGAACTCCATGTCGAGGATGCCGCATTCGCCGACTGA

Upstream 100 bases:

>100_bases
TTTCGCCGGAGCGCGCATCGTATAGCGCAAAAGAATGATACCGGTCACTGCCATAACCCGATCCCTTTCAGGACAAGCCT
GGCGCTGGCGCGGGGGCAAC

Downstream 100 bases:

>100_bases
ACTTTTTTATCATTTCGCCGCTTGACGCCCCGCCATCCTCCCCTTAGAGGCGCGGACCTGCCCAGCGGCGCAAGCCCAGT
GGCCCCTTCGTCTAGCGGTC

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MDLGGAGGIQGGGLSDDIVTQLLLSRGVGREDLDRHRRPTLRDFLPDPSIFQDMDNAARRLTDAILSGERITIYGDYDVD
GATSAALLIRLLRELGVDAGHYIPDRLLEGYGPSGEALVRLAREGSNLIVTVDCGAMAHEALAAAAAEGVDVIVVDHHKC
APELPRAAALVNPNRLDECDEAAAHGHLAAVGVAFLLAVATVRELRGRGFFDGRKAPDLMALLDLVALGTVADVAQLKGL
NRALVSQGLKIMARRENVGLSALIDASRLSRAPTCSDLGFALGPRINAGGRVGEATLGVRLLTTEDPEEARAISAQLSRL
NDERRAIEQAVQEAAEAQVDAQHNRAVMVLAGSGWHPGVIGIVAGRIKEKTGKPTLVIALDADEAGHGKGSGRSISGVDL
GAAIIAAREAGLLVAGGGHAMACGLTIEPSALSRLADWLDERLSRDVTAAQSSQALLLDLSLSPGGLTPDLVETLEAAGP
FGVGWPGPRVAVGPVRLVKCDLVGTDHVRMVAAGADGRSFKAIAFRAAQSELGQVLLNASRGRQFWLAGRARIDDWASRP
AAELHVEDAAFAD

Sequences:

>Translated_573_residues
MDLGGAGGIQGGGLSDDIVTQLLLSRGVGREDLDRHRRPTLRDFLPDPSIFQDMDNAARRLTDAILSGERITIYGDYDVD
GATSAALLIRLLRELGVDAGHYIPDRLLEGYGPSGEALVRLAREGSNLIVTVDCGAMAHEALAAAAAEGVDVIVVDHHKC
APELPRAAALVNPNRLDECDEAAAHGHLAAVGVAFLLAVATVRELRGRGFFDGRKAPDLMALLDLVALGTVADVAQLKGL
NRALVSQGLKIMARRENVGLSALIDASRLSRAPTCSDLGFALGPRINAGGRVGEATLGVRLLTTEDPEEARAISAQLSRL
NDERRAIEQAVQEAAEAQVDAQHNRAVMVLAGSGWHPGVIGIVAGRIKEKTGKPTLVIALDADEAGHGKGSGRSISGVDL
GAAIIAAREAGLLVAGGGHAMACGLTIEPSALSRLADWLDERLSRDVTAAQSSQALLLDLSLSPGGLTPDLVETLEAAGP
FGVGWPGPRVAVGPVRLVKCDLVGTDHVRMVAAGADGRSFKAIAFRAAQSELGQVLLNASRGRQFWLAGRARIDDWASRP
AAELHVEDAAFAD
>Mature_573_residues
MDLGGAGGIQGGGLSDDIVTQLLLSRGVGREDLDRHRRPTLRDFLPDPSIFQDMDNAARRLTDAILSGERITIYGDYDVD
GATSAALLIRLLRELGVDAGHYIPDRLLEGYGPSGEALVRLAREGSNLIVTVDCGAMAHEALAAAAAEGVDVIVVDHHKC
APELPRAAALVNPNRLDECDEAAAHGHLAAVGVAFLLAVATVRELRGRGFFDGRKAPDLMALLDLVALGTVADVAQLKGL
NRALVSQGLKIMARRENVGLSALIDASRLSRAPTCSDLGFALGPRINAGGRVGEATLGVRLLTTEDPEEARAISAQLSRL
NDERRAIEQAVQEAAEAQVDAQHNRAVMVLAGSGWHPGVIGIVAGRIKEKTGKPTLVIALDADEAGHGKGSGRSISGVDL
GAAIIAAREAGLLVAGGGHAMACGLTIEPSALSRLADWLDERLSRDVTAAQSSQALLLDLSLSPGGLTPDLVETLEAAGP
FGVGWPGPRVAVGPVRLVKCDLVGTDHVRMVAAGADGRSFKAIAFRAAQSELGQVLLNASRGRQFWLAGRARIDDWASRP
AAELHVEDAAFAD

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=522, Percent_Identity=37.7394636015326, Blast_Score=270, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 59877; Mature: 59877

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLGGAGGIQGGGLSDDIVTQLLLSRGVGREDLDRHRRPTLRDFLPDPSIFQDMDNAARR
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHH
LTDAILSGERITIYGDYDVDGATSAALLIRLLRELGVDAGHYIPDRLLEGYGPSGEALVR
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHH
LAREGSNLIVTVDCGAMAHEALAAAAAEGVDVIVVDHHKCAPELPRAAALVNPNRLDECD
HHHCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHCCCCCCCCCHHH
EAAAHGHLAAVGVAFLLAVATVRELRGRGFFDGRKAPDLMALLDLVALGTVADVAQLKGL
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
NRALVSQGLKIMARRENVGLSALIDASRLSRAPTCSDLGFALGPRINAGGRVGEATLGVR
HHHHHHCCHHEEHHHCCCCHHHEECHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCEEEEE
LLTTEDPEEARAISAQLSRLNDERRAIEQAVQEAAEAQVDAQHNRAVMVLAGSGWHPGVI
EEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHH
GIVAGRIKEKTGKPTLVIALDADEAGHGKGSGRSISGVDLGAAIIAAREAGLLVAGGGHA
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEECCCHHHHHHHHHHCCCEEEECCCCE
MACGLTIEPSALSRLADWLDERLSRDVTAAQSSQALLLDLSLSPGGLTPDLVETLEAAGP
EEEEEEECHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCC
FGVGWPGPRVAVGPVRLVKCDLVGTDHVRMVAAGADGRSFKAIAFRAAQSELGQVLLNAS
CCCCCCCCCEEECCEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
RGRQFWLAGRARIDDWASRPAAELHVEDAAFAD
CCCEEEEECCCCCCHHHCCCCCEEEECCCCCCC
>Mature Secondary Structure
MDLGGAGGIQGGGLSDDIVTQLLLSRGVGREDLDRHRRPTLRDFLPDPSIFQDMDNAARR
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHH
LTDAILSGERITIYGDYDVDGATSAALLIRLLRELGVDAGHYIPDRLLEGYGPSGEALVR
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHH
LAREGSNLIVTVDCGAMAHEALAAAAAEGVDVIVVDHHKCAPELPRAAALVNPNRLDECD
HHHCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHCCCCCCCCCHHH
EAAAHGHLAAVGVAFLLAVATVRELRGRGFFDGRKAPDLMALLDLVALGTVADVAQLKGL
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
NRALVSQGLKIMARRENVGLSALIDASRLSRAPTCSDLGFALGPRINAGGRVGEATLGVR
HHHHHHCCHHEEHHHCCCCHHHEECHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCEEEEE
LLTTEDPEEARAISAQLSRLNDERRAIEQAVQEAAEAQVDAQHNRAVMVLAGSGWHPGVI
EEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHH
GIVAGRIKEKTGKPTLVIALDADEAGHGKGSGRSISGVDLGAAIIAAREAGLLVAGGGHA
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEECCCHHHHHHHHHHCCCEEEECCCCE
MACGLTIEPSALSRLADWLDERLSRDVTAAQSSQALLLDLSLSPGGLTPDLVETLEAAGP
EEEEEEECHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCC
FGVGWPGPRVAVGPVRLVKCDLVGTDHVRMVAAGADGRSFKAIAFRAAQSELGQVLLNAS
CCCCCCCCCEEECCEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
RGRQFWLAGRARIDDWASRPAAELHVEDAAFAD
CCCEEEEECCCCCCHHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]