Click here to switch to the map view.

The map label for this gene is queA

Identifier: 87200264

GI number: 87200264

Start: 2390270

End: 2391298

Strand: Reverse

Name: queA

Synonym: Saro_2250

Alternate gene names: 87200264

Gene position: 2391298-2390270 (Counterclockwise)

Preceding gene: 87200265

Following gene: 87200263

Centisome position: 67.14

GC content: 66.18

Gene sequence:

>1029_bases
ATGCGCGTTGATCTCTTCGACTTCGAACTGCCGTCCGAAAACATCGCCCTGCGTCCGGCACGGCCTCGCGATTCCGCGAG
GTTGCTTGGCGTCGGCCGCGATGGCCCGTTCCGCGATCTCATCGTGCGGGACCTGCCGGACCTGCTGAACCCCGGCGATG
TTCTGGTGTTCAACGACACCCGCGTGATACCCGCACAGCTCGAAGGACAGCGCGGCGAAGCGCGGGTCGGCGCGACTCTG
CACAAGCGCATCGACCTGCGCCGCTGGCAGGCCTTCGTGCGCAATGCCAAGCGCCTGCGCGAGGGCGACGTGATCCAGTT
CGGGGCCGGTGTCACCGCAACCGCTGAAATGCGCCTGCCGGACGGAAGCTGGACGCTGTTCTTCCCGGGCGAGGAACCGG
TGGAGGTTCTCCTCGAACGCGCTGGCCGCATGCCGCTGCCGCCCTATATCGCGGGCAAGCGGCCGACCGACGAGCAGGAC
CGCGAAGACTATCAGACGATGTTCGCGGCCAAGGACGGAGCGGTTGCCGCGCCAACCGCCGCGCTCCACTTCACCCCGTC
GCTGATGGAAGCGCTGGCGGCGCGCGGCGTGGCAACCGAGACGCTGACGCTGCACGTGGGTGCTGGCACGTTCCTCCCGG
TCAAGGCCGACGACACGCAGGACCACCAGATGCATGCCGAGTGGGGCACCATCGACGCTGCCACCGCAGATCGGCTCAAC
GCAGCCCGAGCCGCCGGCAAACGCGTGATCGCGGTCGGCACCACCAGCTTGCGCCTGCTCGAAAGCGCTACGGGAGAGGA
CCGGGTAATCCGTCCGTTCGAGGGCGACACTTCGATCTTCATTACCCCGGGATATACATTCCGCGCGATCGACGGGCTGA
TGACCAACTTCCACCTGCCCAAGTCGACCCTGTTCATGCTGGTCAGTGCGCTGATGGGTCTCGATCGCATGCAGGCAGCC
TATGCCCACGCCATTGCCGGAGGCTACCGCTTCTACTCCTACGGGGATTCGAGCCTGCTCCTGCCATAA

Upstream 100 bases:

>100_bases
TTCCCGCACCGGCAGCGCCGGCGGCACCGGCTGCGATCACTGCCGACATGCTGAGCAATTCGAAGTCGAACTGAGCCAGG
TTTGGCGCTAGGGCGCGCCC

Downstream 100 bases:

>100_bases
AGCGCAGTTTCGATAAGGCCTGCACCCGGTAGCCTGCCTCCAAACACAAGTGGGAGCAGGGCATGATACCGGCACACGTT
CCGGCCGACCGGGTGGTCGA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRVDLFDFELPSENIALRPARPRDSARLLGVGRDGPFRDLIVRDLPDLLNPGDVLVFNDTRVIPAQLEGQRGEARVGATL
HKRIDLRRWQAFVRNAKRLREGDVIQFGAGVTATAEMRLPDGSWTLFFPGEEPVEVLLERAGRMPLPPYIAGKRPTDEQD
REDYQTMFAAKDGAVAAPTAALHFTPSLMEALAARGVATETLTLHVGAGTFLPVKADDTQDHQMHAEWGTIDAATADRLN
AARAAGKRVIAVGTTSLRLLESATGEDRVIRPFEGDTSIFITPGYTFRAIDGLMTNFHLPKSTLFMLVSALMGLDRMQAA
YAHAIAGGYRFYSYGDSSLLLP

Sequences:

>Translated_342_residues
MRVDLFDFELPSENIALRPARPRDSARLLGVGRDGPFRDLIVRDLPDLLNPGDVLVFNDTRVIPAQLEGQRGEARVGATL
HKRIDLRRWQAFVRNAKRLREGDVIQFGAGVTATAEMRLPDGSWTLFFPGEEPVEVLLERAGRMPLPPYIAGKRPTDEQD
REDYQTMFAAKDGAVAAPTAALHFTPSLMEALAARGVATETLTLHVGAGTFLPVKADDTQDHQMHAEWGTIDAATADRLN
AARAAGKRVIAVGTTSLRLLESATGEDRVIRPFEGDTSIFITPGYTFRAIDGLMTNFHLPKSTLFMLVSALMGLDRMQAA
YAHAIAGGYRFYSYGDSSLLLP
>Mature_342_residues
MRVDLFDFELPSENIALRPARPRDSARLLGVGRDGPFRDLIVRDLPDLLNPGDVLVFNDTRVIPAQLEGQRGEARVGATL
HKRIDLRRWQAFVRNAKRLREGDVIQFGAGVTATAEMRLPDGSWTLFFPGEEPVEVLLERAGRMPLPPYIAGKRPTDEQD
REDYQTMFAAKDGAVAAPTAALHFTPSLMEALAARGVATETLTLHVGAGTFLPVKADDTQDHQMHAEWGTIDAATADRLN
AARAAGKRVIAVGTTSLRLLESATGEDRVIRPFEGDTSIFITPGYTFRAIDGLMTNFHLPKSTLFMLVSALMGLDRMQAA
YAHAIAGGYRFYSYGDSSLLLP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=46.0869565217391, Blast_Score=278, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_NOVAD (Q2G636)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497521.1
- HSSP:   O32054
- ProteinModelPortal:   Q2G636
- SMR:   Q2G636
- STRING:   Q2G636
- GeneID:   3916566
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2250
- NMPDR:   fig|48935.1.peg.1732
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q2G636
- ProtClustDB:   PRK00147
- BioCyc:   NARO279238:SARO_2250-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37518; Mature: 37518

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFELPSENIALRPARPRDSARLLGVGRDGPFRDLIVRDLPDLLNPGDVLVFNDT
CEEEEEECCCCCCCEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCC
RVIPAQLEGQRGEARVGATLHKRIDLRRWQAFVRNAKRLREGDVIQFGAGVTATAEMRLP
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECC
DGSWTLFFPGEEPVEVLLERAGRMPLPPYIAGKRPTDEQDREDYQTMFAAKDGAVAAPTA
CCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
ALHFTPSLMEALAARGVATETLTLHVGAGTFLPVKADDTQDHQMHAEWGTIDAATADRLN
EEEECHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCCCCEEECCCCCEECHHHHHHH
AARAAGKRVIAVGTTSLRLLESATGEDRVIRPFEGDTSIFITPGYTFRAIDGLMTNFHLP
HHHHCCCEEEEECCHHHHHHHCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHCCCC
KSTLFMLVSALMGLDRMQAAYAHAIAGGYRFYSYGDSSLLLP
HHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEECCCEEECC
>Mature Secondary Structure
MRVDLFDFELPSENIALRPARPRDSARLLGVGRDGPFRDLIVRDLPDLLNPGDVLVFNDT
CEEEEEECCCCCCCEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCC
RVIPAQLEGQRGEARVGATLHKRIDLRRWQAFVRNAKRLREGDVIQFGAGVTATAEMRLP
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECC
DGSWTLFFPGEEPVEVLLERAGRMPLPPYIAGKRPTDEQDREDYQTMFAAKDGAVAAPTA
CCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
ALHFTPSLMEALAARGVATETLTLHVGAGTFLPVKADDTQDHQMHAEWGTIDAATADRLN
EEEECHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCCCCEEECCCCCEECHHHHHHH
AARAAGKRVIAVGTTSLRLLESATGEDRVIRPFEGDTSIFITPGYTFRAIDGLMTNFHLP
HHHHCCCEEEEECCHHHHHHHCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHCCCC
KSTLFMLVSALMGLDRMQAAYAHAIAGGYRFYSYGDSSLLLP
HHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA