Click here to switch to the map view.

The map label for this gene is tgt

Identifier: 87200261

GI number: 87200261

Start: 2387062

End: 2388186

Strand: Reverse

Name: tgt

Synonym: Saro_2247

Alternate gene names: 87200261

Gene position: 2388186-2387062 (Counterclockwise)

Preceding gene: 87200263

Following gene: 87200258

Centisome position: 67.05

GC content: 65.78

Gene sequence:

>1125_bases
ATGACCCGTTTTTCGTTCAAGATCCACGCCACCGACGGCAAGGCCCGTACCGGTGCCATCCAGATGATGCGCGGCGAGAT
TCGCACGCCTGCGTTCATGCCGGTTGGTACCGCCGCCACCGTCAAGGCGATGAAGGTCGAGGACGTGCGGGCTTCGGGTG
CGGACATAATCCTCGGCAACACCTATCACCTGATGCTGCGCCCAGGGGCCGAGCGCGTCGCGCGGCTTGGCGGTCTGCAC
AAGTTCATGGGTTGGGACCGCCCGATCCTTACCGACAGCGGCGGCTATCAGGTGATGAGCCTGTCCGACCTGCGCAAGAT
CACCGAGGAGGGGGTGACCTTCGCCAGCCACCTCGACGGCTCGCGCCACCTGCTCAGCCCGGAACGGTCGATGGAGATCC
AGCGCCTGCTCGGCTCCGACATCGTCATGTGCTTCGACGAATGTCCGCGAGCCGATCAGCCGCGCGAGGTGATCGCGAGG
TCGATGGAAATGTCGATGCGTTGGGCGAGGCGCAGCCGCGACGCGTTCGACGCGGGCGGGGAACATGCGGAACGCTCCGC
ACTCTTCGGCATCCAGCAGGGCGCTCTCGACGAAGGGTTGCGCAAGACCAGCGCCGATGCGCTGACCGACATCGGGTTCG
ATGGCTATGCGATCGGAGGCCTTGCTGTGGGCGAGGGGCAGGAGGCGATGTTCGCCACGCTCGATTTCGCGCCCCAGCAG
CTACCCGCTGATCGGCCGCGCTATCTCATGGGTGTCGGCAAGCCCGACGACCTGGTGGGCGCGGTGGAGCGCGGCGTCGA
CATGTTCGATTGCGTCCTGCCGACGCGTTCCGGGCGCAATGGCCAGGCCTTCACCTGGAACGGCCCACTGAACATGCGCA
ACGCCCGCCATGCCGAGGACACCGGCCCGCTCGACGAACGTTGCCCGTGCCCGACCTGTACGAAGTACAGCCGGGCATAC
CTGCATCACCTGCACAAGTCGGGCGAGATGCTTGGGGCCATGCTGCTGACCGAACACAACCTGTGGTTCTACCAGCAGCT
CATGGCTGGGATGCGCGCGGCGATTGCCGAAGGTCGGTTCGCCGCCTTTGCCGCTGACTTCCGCCGCGACTACTTCGCGC
GTTAG

Upstream 100 bases:

>100_bases
GATAGAACCACAGGTCGAGATGTGCAGGTTCGAGCATTTGCGCCCATTGCCGATTGCGCCTAAGGGCCGCAACATTCTTT
TTGCCGAGCGCCTGACCCAG

Downstream 100 bases:

>100_bases
GCCGCGATCTCTTGCGGCCCGTCGGCAAGATCGGGCTCGCTCACTTCCAGCCGCGTATCCTTGTACGCATAGAACGTGCC
CGTTCGCTCGTCCCGAAAGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MTRFSFKIHATDGKARTGAIQMMRGEIRTPAFMPVGTAATVKAMKVEDVRASGADIILGNTYHLMLRPGAERVARLGGLH
KFMGWDRPILTDSGGYQVMSLSDLRKITEEGVTFASHLDGSRHLLSPERSMEIQRLLGSDIVMCFDECPRADQPREVIAR
SMEMSMRWARRSRDAFDAGGEHAERSALFGIQQGALDEGLRKTSADALTDIGFDGYAIGGLAVGEGQEAMFATLDFAPQQ
LPADRPRYLMGVGKPDDLVGAVERGVDMFDCVLPTRSGRNGQAFTWNGPLNMRNARHAEDTGPLDERCPCPTCTKYSRAY
LHHLHKSGEMLGAMLLTEHNLWFYQQLMAGMRAAIAEGRFAAFAADFRRDYFAR

Sequences:

>Translated_374_residues
MTRFSFKIHATDGKARTGAIQMMRGEIRTPAFMPVGTAATVKAMKVEDVRASGADIILGNTYHLMLRPGAERVARLGGLH
KFMGWDRPILTDSGGYQVMSLSDLRKITEEGVTFASHLDGSRHLLSPERSMEIQRLLGSDIVMCFDECPRADQPREVIAR
SMEMSMRWARRSRDAFDAGGEHAERSALFGIQQGALDEGLRKTSADALTDIGFDGYAIGGLAVGEGQEAMFATLDFAPQQ
LPADRPRYLMGVGKPDDLVGAVERGVDMFDCVLPTRSGRNGQAFTWNGPLNMRNARHAEDTGPLDERCPCPTCTKYSRAY
LHHLHKSGEMLGAMLLTEHNLWFYQQLMAGMRAAIAEGRFAAFAADFRRDYFAR
>Mature_373_residues
TRFSFKIHATDGKARTGAIQMMRGEIRTPAFMPVGTAATVKAMKVEDVRASGADIILGNTYHLMLRPGAERVARLGGLHK
FMGWDRPILTDSGGYQVMSLSDLRKITEEGVTFASHLDGSRHLLSPERSMEIQRLLGSDIVMCFDECPRADQPREVIARS
MEMSMRWARRSRDAFDAGGEHAERSALFGIQQGALDEGLRKTSADALTDIGFDGYAIGGLAVGEGQEAMFATLDFAPQQL
PADRPRYLMGVGKPDDLVGAVERGVDMFDCVLPTRSGRNGQAFTWNGPLNMRNARHAEDTGPLDERCPCPTCTKYSRAYL
HHLHKSGEMLGAMLLTEHNLWFYQQLMAGMRAAIAEGRFAAFAADFRRDYFAR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=354, Percent_Identity=44.9152542372881, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1786607, Length=363, Percent_Identity=57.5757575757576, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17544680, Length=368, Percent_Identity=40.2173913043478, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI25146502, Length=217, Percent_Identity=23.963133640553, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19920510, Length=373, Percent_Identity=42.627345844504, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI21355311, Length=155, Percent_Identity=29.6774193548387, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_NOVAD (Q2G639)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497518.1
- ProteinModelPortal:   Q2G639
- SMR:   Q2G639
- STRING:   Q2G639
- GeneID:   3916563
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2247
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q2G639
- ProtClustDB:   PRK00112
- BioCyc:   NARO279238:SARO_2247-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41390; Mature: 41259

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: ACT_SITE 92-92 BINDING 93-93

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFSFKIHATDGKARTGAIQMMRGEIRTPAFMPVGTAATVKAMKVEDVRASGADIILGN
CCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEHHHHHCCCCCEEECC
TYHLMLRPGAERVARLGGLHKFMGWDRPILTDSGGYQVMSLSDLRKITEEGVTFASHLDG
EEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHCCEEHHHCCC
SRHLLSPERSMEIQRLLGSDIVMCFDECPRADQPREVIARSMEMSMRWARRSRDAFDAGG
CCCCCCCHHHHHHHHHHCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCC
EHAERSALFGIQQGALDEGLRKTSADALTDIGFDGYAIGGLAVGEGQEAMFATLDFAPQQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCEEEEEECCCHHH
LPADRPRYLMGVGKPDDLVGAVERGVDMFDCVLPTRSGRNGQAFTWNGPLNMRNARHAED
CCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCC
TGPLDERCPCPTCTKYSRAYLHHLHKSGEMLGAMLLTEHNLWFYQQLMAGMRAAIAEGRF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
AAFAADFRRDYFAR
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TRFSFKIHATDGKARTGAIQMMRGEIRTPAFMPVGTAATVKAMKVEDVRASGADIILGN
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEHHHHHCCCCCEEECC
TYHLMLRPGAERVARLGGLHKFMGWDRPILTDSGGYQVMSLSDLRKITEEGVTFASHLDG
EEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHCCEEHHHCCC
SRHLLSPERSMEIQRLLGSDIVMCFDECPRADQPREVIARSMEMSMRWARRSRDAFDAGG
CCCCCCCHHHHHHHHHHCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCC
EHAERSALFGIQQGALDEGLRKTSADALTDIGFDGYAIGGLAVGEGQEAMFATLDFAPQQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCEEEEEECCCHHH
LPADRPRYLMGVGKPDDLVGAVERGVDMFDCVLPTRSGRNGQAFTWNGPLNMRNARHAED
CCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCC
TGPLDERCPCPTCTKYSRAYLHHLHKSGEMLGAMLLTEHNLWFYQQLMAGMRAAIAEGRF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
AAFAADFRRDYFAR
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA