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The map label for this gene is pth

Identifier: 87200132

GI number: 87200132

Start: 2254975

End: 2255550

Strand: Direct

Name: pth

Synonym: Saro_2116

Alternate gene names: 87200132

Gene position: 2254975-2255550 (Clockwise)

Preceding gene: 87200131

Following gene: 87200133

Centisome position: 63.31

GC content: 60.94

Gene sequence:

>576_bases
ATGCAGCTCTGGGTCGGCCTCGGAAATCCCGGACCGCAATATGCGCTCCACCGGCACAATGTCGGCTTCATGGCGCTCGA
CACTATTGCGGAGGTGCACAATTTCGGCCCGGTCCAGAAGAAGTTCCAGGGCTGGTTGCAGGAGGGCCGCATCGGCACCG
AAAAGGTCCTGCTGCTCAAGCCCGCGACGTTCATGAACGAAAGCGGTCGTTCCGTCGGCGAGGCGATGCGCTTCTACAAG
CTTGGGATGGATGCTCTGGTCGTGTTCCACGACGAGCTGGACCTCGCCCCGTTCAAGGTGAAGGTGAAGCAGGGCGGCGG
TACGGCGGGCCACAACGGATTGCGTTCCATCGACAAGCACCTCGGGCCGGACTTCCTGCGGGTGCGGCTCGGCATCGGCC
ATCCCGGGCACAAGGACCGGGTGACGGGCCACGTCCTGGGCAACTTCGCCAAGTCCGAACAGGACGATCTCGTCGACATG
CTCGGCGCAATTGCATCCGAAGCGGCCCTTTTAGCCAAGGGTGATAATGTACTTTTCATGAATAACATTGCACTTCGTCA
ACAAACGGCGGGGTGA

Upstream 100 bases:

>100_bases
CTTCCGCCGCGTGCGGGAGGGGTTCTTTTTTTGCCGGGCCATATCTGGCAATGCGCCCCAGTCCCAAATGGCCAGCACCG
AATGAAAGGATCAATCCCGA

Downstream 100 bases:

>100_bases
AGCAACAATTCGGAAACGTGACTTGTCGAACGCCTTTACGCCATTAATAATGGTTAACGCGCCGGACACTATAGTTGCTG
CATTTTTCGATTTGGCACCG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MQLWVGLGNPGPQYALHRHNVGFMALDTIAEVHNFGPVQKKFQGWLQEGRIGTEKVLLLKPATFMNESGRSVGEAMRFYK
LGMDALVVFHDELDLAPFKVKVKQGGGTAGHNGLRSIDKHLGPDFLRVRLGIGHPGHKDRVTGHVLGNFAKSEQDDLVDM
LGAIASEAALLAKGDNVLFMNNIALRQQTAG

Sequences:

>Translated_191_residues
MQLWVGLGNPGPQYALHRHNVGFMALDTIAEVHNFGPVQKKFQGWLQEGRIGTEKVLLLKPATFMNESGRSVGEAMRFYK
LGMDALVVFHDELDLAPFKVKVKQGGGTAGHNGLRSIDKHLGPDFLRVRLGIGHPGHKDRVTGHVLGNFAKSEQDDLVDM
LGAIASEAALLAKGDNVLFMNNIALRQQTAG
>Mature_191_residues
MQLWVGLGNPGPQYALHRHNVGFMALDTIAEVHNFGPVQKKFQGWLQEGRIGTEKVLLLKPATFMNESGRSVGEAMRFYK
LGMDALVVFHDELDLAPFKVKVKQGGGTAGHNGLRSIDKHLGPDFLRVRLGIGHPGHKDRVTGHVLGNFAKSEQDDLVDM
LGAIASEAALLAKGDNVLFMNNIALRQQTAG

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=161, Percent_Identity=36.0248447204969, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1787455, Length=166, Percent_Identity=46.3855421686747, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321983, Length=168, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_NOVAD (Q2G6G8)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497389.1
- ProteinModelPortal:   Q2G6G8
- SMR:   Q2G6G8
- STRING:   Q2G6G8
- GeneID:   3917764
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2116
- NMPDR:   fig|48935.1.peg.491
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- PhylomeDB:   Q2G6G8
- ProtClustDB:   PRK05426
- BioCyc:   NARO279238:SARO_2116-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20818; Mature: 20818

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLWVGLGNPGPQYALHRHNVGFMALDTIAEVHNFGPVQKKFQGWLQEGRIGTEKVLLLK
CEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEC
PATFMNESGRSVGEAMRFYKLGMDALVVFHDELDLAPFKVKVKQGGGTAGHNGLRSIDKH
CHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHH
LGPDFLRVRLGIGHPGHKDRVTGHVLGNFAKSEQDDLVDMLGAIASEAALLAKGDNVLFM
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCEEEE
NNIALRQQTAG
CCEEHHCCCCC
>Mature Secondary Structure
MQLWVGLGNPGPQYALHRHNVGFMALDTIAEVHNFGPVQKKFQGWLQEGRIGTEKVLLLK
CEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEC
PATFMNESGRSVGEAMRFYKLGMDALVVFHDELDLAPFKVKVKQGGGTAGHNGLRSIDKH
CHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHH
LGPDFLRVRLGIGHPGHKDRVTGHVLGNFAKSEQDDLVDMLGAIASEAALLAKGDNVLFM
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCEEEE
NNIALRQQTAG
CCEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA