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The map label for this gene is ligF [H]

Identifier: 87200107

GI number: 87200107

Start: 2227684

End: 2228457

Strand: Reverse

Name: ligF [H]

Synonym: Saro_2091

Alternate gene names: 87200107

Gene position: 2228457-2227684 (Counterclockwise)

Preceding gene: 87200114

Following gene: 87200105

Centisome position: 62.57

GC content: 61.89

Gene sequence:

>774_bases
ATGGTGATTCCACTCGGAGAGGATAACACCATCATGCTCAAGCTCTACTCCTTCGGACCTGCCGCCAATTCGATGAAGCC
GCTGCTGACGGTGTTCGAGAAGGGCCTCGATGTCGAAAAGCACCGGCTCGACCCCGCCAAGTTCGAGCATCACACCGACT
GGTTCAAGGCCATCAACCCGCGCGGGCAGGTGCCGGCACTGGTGGACGGCGACAAGGTGGTGACCGAATCCACCGTGATC
TGCGAATATCTCGAGGATGAATATCCCACCGAGGTCGCGCTCCGCCCCGCCGACAGCTTCGGCAAGGCGCAGATGCGCAT
CTGGACCAAGTGGGTGGACGAATATTTCTGCTGGTGCGTCTCGACCATCGGCTGGCACCGCTATGTCGGCAACATGGTCA
AGAGCCTGAGCGACGCGGAGTTCGAGGAGAAGGTCAAGGCGATCCCCGTGATCGAGCAGCAGGTGAAGTGGCGCCGCGCG
CGCGAAGGCTTCCCGCAGGACATGCTCGACGAAGAAATGCGCAAGATCGCCTACTCCGTGCGCAAGCTCGACGACCACCT
GGCCGACCACGAGTGGCTGGTTCCCGGGCAGTACACCCTGGCCGACATCTGCAACTTCGCGATCGCCAACGGCATGCAGT
TCGGCTTTGCCGAACTGGTCAACAAGCAGGACACGCCGCACCTGGTCCGCTGGATCGAGCAGATCAACGAGCGGCCCGCG
GTGAAGCAGATGTTCGCGCAGGTCGAACTGGAAAAGCTCGGGCCGCGCGAGTGA

Upstream 100 bases:

>100_bases
GTGACCGCCCCGCAGGCGCAGCTTCCAGTGTAGGTCATCAGTGCAGTCTCCCCGAGCATGGAGCCAATTTTGATATTGTT
AGTGTTACATACAATTGGCT

Downstream 100 bases:

>100_bases
GGCGACAGTGTCCCCGGCCCCTCTCCTGAACGGGAGGGGGGAAGGGGCGCCAGCCGGCATCAGTTGCCCGGGCGGCGGGC
CATGAAGGCCAGCTTTTCGA

Product: glutathione S-transferase-like protein

Products: HX; R-S-Glutathione; S-(2,4-dinitrophenyl)glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MVIPLGEDNTIMLKLYSFGPAANSMKPLLTVFEKGLDVEKHRLDPAKFEHHTDWFKAINPRGQVPALVDGDKVVTESTVI
CEYLEDEYPTEVALRPADSFGKAQMRIWTKWVDEYFCWCVSTIGWHRYVGNMVKSLSDAEFEEKVKAIPVIEQQVKWRRA
REGFPQDMLDEEMRKIAYSVRKLDDHLADHEWLVPGQYTLADICNFAIANGMQFGFAELVNKQDTPHLVRWIEQINERPA
VKQMFAQVELEKLGPRE

Sequences:

>Translated_257_residues
MVIPLGEDNTIMLKLYSFGPAANSMKPLLTVFEKGLDVEKHRLDPAKFEHHTDWFKAINPRGQVPALVDGDKVVTESTVI
CEYLEDEYPTEVALRPADSFGKAQMRIWTKWVDEYFCWCVSTIGWHRYVGNMVKSLSDAEFEEKVKAIPVIEQQVKWRRA
REGFPQDMLDEEMRKIAYSVRKLDDHLADHEWLVPGQYTLADICNFAIANGMQFGFAELVNKQDTPHLVRWIEQINERPA
VKQMFAQVELEKLGPRE
>Mature_257_residues
MVIPLGEDNTIMLKLYSFGPAANSMKPLLTVFEKGLDVEKHRLDPAKFEHHTDWFKAINPRGQVPALVDGDKVVTESTVI
CEYLEDEYPTEVALRPADSFGKAQMRIWTKWVDEYFCWCVSTIGWHRYVGNMVKSLSDAEFEEKVKAIPVIEQQVKWRRA
REGFPQDMLDEEMRKIAYSVRKLDDHLADHEWLVPGQYTLADICNFAIANGMQFGFAELVNKQDTPHLVRWIEQINERPA
VKQMFAQVELEKLGPRE

Specific function: Lignin degradation enzyme [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18 [C]

Molecular weight: Translated: 29759; Mature: 29759

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIPLGEDNTIMLKLYSFGPAANSMKPLLTVFEKGLDVEKHRLDPAKFEHHTDWFKAINP
CEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCC
RGQVPALVDGDKVVTESTVICEYLEDEYPTEVALRPADSFGKAQMRIWTKWVDEYFCWCV
CCCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
STIGWHRYVGNMVKSLSDAEFEEKVKAIPVIEQQVKWRRAREGFPQDMLDEEMRKIAYSV
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RKLDDHLADHEWLVPGQYTLADICNFAIANGMQFGFAELVNKQDTPHLVRWIEQINERPA
HHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHH
VKQMFAQVELEKLGPRE
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVIPLGEDNTIMLKLYSFGPAANSMKPLLTVFEKGLDVEKHRLDPAKFEHHTDWFKAINP
CEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCC
RGQVPALVDGDKVVTESTVICEYLEDEYPTEVALRPADSFGKAQMRIWTKWVDEYFCWCV
CCCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
STIGWHRYVGNMVKSLSDAEFEEKVKAIPVIEQQVKWRRAREGFPQDMLDEEMRKIAYSV
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RKLDDHLADHEWLVPGQYTLADICNFAIANGMQFGFAELVNKQDTPHLVRWIEQINERPA
HHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHH
VKQMFAQVELEKLGPRE
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; Glutathione; 1-chloro-2,4-dinitrobenzene; reduced glutathione [C]

Specific reaction: RX + Glutathione = HX + R-S-Glutathione 1-chloro-2,4-dinitrobenzene + reduced glutathione = S-(2,4-dinitrophenyl)glutathione [C]

General reaction: Aryl group transfer [C]

Inhibitor: Cd2+; CibacronBlue; Co2+; Cu2+; Hematin; Hg2+; Ni2+; Sulfophthaleins; Tributyl tinacetate; Triethyl tinbromide; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726 [H]