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The map label for this gene is lipA

Identifier: 87199938

GI number: 87199938

Start: 2034189

End: 2035121

Strand: Direct

Name: lipA

Synonym: Saro_1921

Alternate gene names: 87199938

Gene position: 2034189-2035121 (Clockwise)

Preceding gene: 87199936

Following gene: 87199939

Centisome position: 57.11

GC content: 63.99

Gene sequence:

>933_bases
ATGACGAACCCTGCCGAAACCGCCCGTCCCGAACGCCAGCGCAAGCCGGACTGGATCCGCGTAAAGGCCCCGACCAGCAA
GGGCTATGGCGAGACGCGCGCCCTCATGCGCGAACTGGGACTCAACACGGTCTGTGAAGAGGCAGCCTGTCCCAACATCG
GCGAATGCTGGACCAAGAAGCACGCCACGGTAATGATCCTGGGCGATGTGTGCACGCGCGCTTGCGCGTTCTGCAACGTC
AAGACCGGCATGCCGCGCGCCGTCGATCCGATGGAGCCGATCAATGTCGCCACGGCGGCAGCTAAGATGGGCCTCGAGCA
CATCGTCATCACGTCGGTCGACCGCGATGATCTGCCGGATGGCGGGGCAGGGCACTTCGTCAAGGTCATCAAGGCCCTGC
GCGAGTTGACACCGAACACCACCATAGAAATCCTGACGCCCGACTTCCGGAACAAGATGGAAGCGGCGGTGGAATCGATC
GTCGAGGCGGGACCCGACGTCTACAACCACAACCTCGAGACGGTTCCCCGCCTTTACCCGACCATTCGTCCGGGCGCGCG
CTATTACGCGTCGCTGCGCTTGCTCGAACAGGTCAAGCGCCACGATCCGCGCATCTTCACCAAGTCCGGCGTCATGCTCG
GGCTGGGCGAGGAGCGGCTAGAAGTCCATCAGGTGATGGATGACATGCGCTCGGCCCAGATCGATTTCCTGACGATGGGG
CAGTATCTCCAGCCCACGCCCAAGCACGCCAAGGTGATCGAGTTCGTCACACCCAAAGCGTTCGATGCCTATGGCTCGAT
CGCGCGGGCCAAGGGCTTCCTCCAGGTCGCGGCGAGCCCGCTGACCCGGTCGAGCTATCACGCGGGCGAGGATTTCCGCC
AGATGCGGCAGGCGCGCGAGGCGCAGCTCGCGAAGGCCGCTCAGAAGGCCTGA

Upstream 100 bases:

>100_bases
GCCGATCGAGCTCGGGGGATGCATCCTGGGTCATGGGCCGTGCTAATGGCCCCATCGATTGGGTCTTTCAAGTGCTTGCG
GATCGGCCTATCTGGGCGCC

Downstream 100 bases:

>100_bases
CCTTGCCCCGCATCGTCGAAACCCGCCGGCTCCAGTGGTCGGCCGAACAGATGTTCGACCTCGTGGCTGACGTGCGCCGC
TATGCCGAGTTCCTGCCCTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTNPAETARPERQRKPDWIRVKAPTSKGYGETRALMRELGLNTVCEEAACPNIGECWTKKHATVMILGDVCTRACAFCNV
KTGMPRAVDPMEPINVATAAAKMGLEHIVITSVDRDDLPDGGAGHFVKVIKALRELTPNTTIEILTPDFRNKMEAAVESI
VEAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLEQVKRHDPRIFTKSGVMLGLGEERLEVHQVMDDMRSAQIDFLTMG
QYLQPTPKHAKVIEFVTPKAFDAYGSIARAKGFLQVAASPLTRSSYHAGEDFRQMRQAREAQLAKAAQKA

Sequences:

>Translated_310_residues
MTNPAETARPERQRKPDWIRVKAPTSKGYGETRALMRELGLNTVCEEAACPNIGECWTKKHATVMILGDVCTRACAFCNV
KTGMPRAVDPMEPINVATAAAKMGLEHIVITSVDRDDLPDGGAGHFVKVIKALRELTPNTTIEILTPDFRNKMEAAVESI
VEAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLEQVKRHDPRIFTKSGVMLGLGEERLEVHQVMDDMRSAQIDFLTMG
QYLQPTPKHAKVIEFVTPKAFDAYGSIARAKGFLQVAASPLTRSSYHAGEDFRQMRQAREAQLAKAAQKA
>Mature_309_residues
TNPAETARPERQRKPDWIRVKAPTSKGYGETRALMRELGLNTVCEEAACPNIGECWTKKHATVMILGDVCTRACAFCNVK
TGMPRAVDPMEPINVATAAAKMGLEHIVITSVDRDDLPDGGAGHFVKVIKALRELTPNTTIEILTPDFRNKMEAAVESIV
EAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLEQVKRHDPRIFTKSGVMLGLGEERLEVHQVMDDMRSAQIDFLTMGQ
YLQPTPKHAKVIEFVTPKAFDAYGSIARAKGFLQVAASPLTRSSYHAGEDFRQMRQAREAQLAKAAQKA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=47.0588235294118, Blast_Score=281, Evalue=6e-76,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=47.7911646586345, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI1786846, Length=283, Percent_Identity=50.1766784452297, Blast_Score=287, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=257, Percent_Identity=49.4163424124514, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324770, Length=300, Percent_Identity=46.6666666666667, Blast_Score=281, Evalue=1e-76,
Organism=Drosophila melanogaster, GI221513272, Length=294, Percent_Identity=48.2993197278912, Blast_Score=292, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_NOVAD (Q2G712)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497195.1
- ProteinModelPortal:   Q2G712
- STRING:   Q2G712
- GeneID:   3917144
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1921
- NMPDR:   fig|48935.1.peg.892
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q2G712
- ProtClustDB:   PRK05481
- BioCyc:   NARO279238:SARO_1921-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34418; Mature: 34287

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPAETARPERQRKPDWIRVKAPTSKGYGETRALMRELGLNTVCEEAACPNIGECWTKK
CCCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCC
HATVMILGDVCTRACAFCNVKTGMPRAVDPMEPINVATAAAKMGLEHIVITSVDRDDLPD
CCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCC
GGAGHFVKVIKALRELTPNTTIEILTPDFRNKMEAAVESIVEAGPDVYNHNLETVPRLYP
CCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHCC
TIRPGARYYASLRLLEQVKRHDPRIFTKSGVMLGLGEERLEVHQVMDDMRSAQIDFLTMG
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCEECC
QYLQPTPKHAKVIEFVTPKAFDAYGSIARAKGFLQVAASPLTRSSYHAGEDFRQMRQARE
HHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AQLAKAAQKA
HHHHHHHHCC
>Mature Secondary Structure 
TNPAETARPERQRKPDWIRVKAPTSKGYGETRALMRELGLNTVCEEAACPNIGECWTKK
CCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCC
HATVMILGDVCTRACAFCNVKTGMPRAVDPMEPINVATAAAKMGLEHIVITSVDRDDLPD
CCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCC
GGAGHFVKVIKALRELTPNTTIEILTPDFRNKMEAAVESIVEAGPDVYNHNLETVPRLYP
CCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHCC
TIRPGARYYASLRLLEQVKRHDPRIFTKSGVMLGLGEERLEVHQVMDDMRSAQIDFLTMG
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCEECC
QYLQPTPKHAKVIEFVTPKAFDAYGSIARAKGFLQVAASPLTRSSYHAGEDFRQMRQARE
HHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AQLAKAAQKA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA