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The map label for this gene is gutB [H]

Identifier: 87199891

GI number: 87199891

Start: 1975363

End: 1976370

Strand: Direct

Name: gutB [H]

Synonym: Saro_1874

Alternate gene names: 87199891

Gene position: 1975363-1976370 (Clockwise)

Preceding gene: 87199890

Following gene: 87199892

Centisome position: 55.46

GC content: 66.47

Gene sequence:

>1008_bases
ATGCGCGCCGCCGTGATGCAGGGCCTGCACAAGCCGCTGGCCATCGATACGATCCCCGATCCGACGCCGGGCGAGGGTGA
TGTCGTGGTCAAGGTCGGGCGCTGCGGCATCTGCGGGTCCGACCTGCACATGACCGAGGACCCGGCATACGGGCAGGGCG
CGGGTTCGGTGCTGGGTCACGAGTTTGCGGGCGAAGTCGTTGCGCTGGGCAAGGGCGTGGAGGGCCTCAGGACCGGGGAT
CTCGTCTCGGTCATCCCGTTACAAAGTTGCGGTCAATGTCATTCGTGTCGCACGGGCGAAGTGCAGTGGTGCGAGAGGTT
CGGTCTTCAGGGTGGCGGCTATGCCGAATTTGCCCTGACGCGGCCGAACCAGTGCGTGCGCCTGCCGGCAAGCGCCAGCA
TGGCTGATGGCGCCATCGTCGAACCGCTAGCCGTGGCTCTGCACGGCCTGGCGCTTAGCCGGATGAAGATCGGCGACAAG
GTGCTGGTGCTGGGCGCGGGACCGATCGGTCTCGCCGTCGCGTTCTGGGCCCGGCGCTTCGGGGCTGGGCGCGTGGTGGT
GCAGGACCTGGCGGAGTGGCAGCGCGACCGCGCTTTGCAGATGGGCGCGCACGATTTCGTCGTCGATGCGGCCGATCCGG
TGGGGAGCGCCGGGCGCGCGCTGGGCGGCAAGGCCGATATAGTATTCGAATGCGTGGGCGTGCCGGGACTGATCGCGCAG
GCTGTGGAGCAGGTGCGCAATGACGGGACCATCACGCTGCTTGGCCTGTGTACGCGGCCCGATACGTTCAACAGCTTCGC
GATGCTGTCCAAGCAGGTGAAGCTGGTGACGTCCGCGTTCTTCACCAGGCAGGAATACGAAGCGGCGCTCGACGCCCTCG
TCCGTGGTGCGGTGGAGCCGCGCCTTCTGGTAACCGACACCATTTCGCTCGATGCAACGCCGGACGTGTTCGAGAGCCTG
CGCAAGCGCACGCATCAGTGCAAGGTGCTGATAAATCCGGGCGAATGA

Upstream 100 bases:

>100_bases
GGCGCTGCTGCTGGCGTCGGACGCTTCGGACTACATGACCGGCAGCCACGTGGTGATCGATGGCGGCATGATGCTCGGCA
AGTACAAGTAAGGAAACCCC

Downstream 100 bases:

>100_bases
TCGATCCCCGGCAAGCTGGAGCAGGGGCCCATGGGCATTGAAACCTTCGCGGGACGAACCGCATTCGTCACCGGTGGAGC
ATCCGGAATAGGCCTGGGCA

Product: GroES-related

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRAAVMQGLHKPLAIDTIPDPTPGEGDVVVKVGRCGICGSDLHMTEDPAYGQGAGSVLGHEFAGEVVALGKGVEGLRTGD
LVSVIPLQSCGQCHSCRTGEVQWCERFGLQGGGYAEFALTRPNQCVRLPASASMADGAIVEPLAVALHGLALSRMKIGDK
VLVLGAGPIGLAVAFWARRFGAGRVVVQDLAEWQRDRALQMGAHDFVVDAADPVGSAGRALGGKADIVFECVGVPGLIAQ
AVEQVRNDGTITLLGLCTRPDTFNSFAMLSKQVKLVTSAFFTRQEYEAALDALVRGAVEPRLLVTDTISLDATPDVFESL
RKRTHQCKVLINPGE

Sequences:

>Translated_335_residues
MRAAVMQGLHKPLAIDTIPDPTPGEGDVVVKVGRCGICGSDLHMTEDPAYGQGAGSVLGHEFAGEVVALGKGVEGLRTGD
LVSVIPLQSCGQCHSCRTGEVQWCERFGLQGGGYAEFALTRPNQCVRLPASASMADGAIVEPLAVALHGLALSRMKIGDK
VLVLGAGPIGLAVAFWARRFGAGRVVVQDLAEWQRDRALQMGAHDFVVDAADPVGSAGRALGGKADIVFECVGVPGLIAQ
AVEQVRNDGTITLLGLCTRPDTFNSFAMLSKQVKLVTSAFFTRQEYEAALDALVRGAVEPRLLVTDTISLDATPDVFESL
RKRTHQCKVLINPGE
>Mature_335_residues
MRAAVMQGLHKPLAIDTIPDPTPGEGDVVVKVGRCGICGSDLHMTEDPAYGQGAGSVLGHEFAGEVVALGKGVEGLRTGD
LVSVIPLQSCGQCHSCRTGEVQWCERFGLQGGGYAEFALTRPNQCVRLPASASMADGAIVEPLAVALHGLALSRMKIGDK
VLVLGAGPIGLAVAFWARRFGAGRVVVQDLAEWQRDRALQMGAHDFVVDAADPVGSAGRALGGKADIVFECVGVPGLIAQ
AVEQVRNDGTITLLGLCTRPDTFNSFAMLSKQVKLVTSAFFTRQEYEAALDALVRGAVEPRLLVTDTISLDATPDVFESL
RKRTHQCKVLINPGE

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=329, Percent_Identity=29.1793313069909, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI262073058, Length=286, Percent_Identity=25.1748251748252, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI71743840, Length=286, Percent_Identity=25.1748251748252, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4501933, Length=264, Percent_Identity=25.3787878787879, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4501929, Length=262, Percent_Identity=24.0458015267176, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI156523966, Length=363, Percent_Identity=21.763085399449, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI4501939, Length=363, Percent_Identity=21.763085399449, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI34577061, Length=264, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI71565154, Length=274, Percent_Identity=27.007299270073, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1787863, Length=337, Percent_Identity=29.3768545994065, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790045, Length=315, Percent_Identity=28.8888888888889, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788073, Length=320, Percent_Identity=30.625, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI226510992, Length=305, Percent_Identity=27.8688524590164, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790718, Length=305, Percent_Identity=28.8524590163934, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082125, Length=352, Percent_Identity=26.9886363636364, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788075, Length=329, Percent_Identity=26.4437689969605, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788407, Length=327, Percent_Identity=27.82874617737, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786825, Length=401, Percent_Identity=24.1895261845387, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=28.8828337874659, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081918, Length=309, Percent_Identity=25.2427184466019, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786518, Length=280, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082401, Length=329, Percent_Identity=25.8358662613982, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=342, Percent_Identity=30.9941520467836, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17562878, Length=330, Percent_Identity=31.5151515151515, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71988145, Length=340, Percent_Identity=28.8235294117647, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17562584, Length=343, Percent_Identity=25.6559766763848, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=337, Percent_Identity=26.1127596439169, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25146526, Length=279, Percent_Identity=27.2401433691756, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71997431, Length=281, Percent_Identity=27.0462633451957, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17565904, Length=221, Percent_Identity=26.6968325791855, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319955, Length=323, Percent_Identity=29.4117647058824, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322619, Length=323, Percent_Identity=29.4117647058824, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6319257, Length=351, Percent_Identity=26.4957264957265, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323099, Length=328, Percent_Identity=27.7439024390244, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6319258, Length=325, Percent_Identity=25.8461538461538, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324486, Length=329, Percent_Identity=27.0516717325228, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=321, Percent_Identity=27.1028037383178, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=343, Percent_Identity=25.3644314868805, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=321, Percent_Identity=26.791277258567, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=275, Percent_Identity=26.9090909090909, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137530, Length=332, Percent_Identity=28.9156626506024, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17737897, Length=334, Percent_Identity=29.0419161676647, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI45551930, Length=338, Percent_Identity=28.698224852071, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550770, Length=338, Percent_Identity=28.698224852071, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI221457811, Length=338, Percent_Identity=28.698224852071, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17737895, Length=301, Percent_Identity=28.2392026578073, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 35327; Mature: 35327

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAVMQGLHKPLAIDTIPDPTPGEGDVVVKVGRCGICGSDLHMTEDPAYGQGAGSVLGH
CCCHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
EFAGEVVALGKGVEGLRTGDLVSVIPLQSCGQCHSCRTGEVQWCERFGLQGGGYAEFALT
HHCCCEEEECCCCCCCCCCCEEEEECCHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEE
RPNQCVRLPASASMADGAIVEPLAVALHGLALSRMKIGDKVLVLGAGPIGLAVAFWARRF
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHHHHH
GAGRVVVQDLAEWQRDRALQMGAHDFVVDAADPVGSAGRALGGKADIVFECVGVPGLIAQ
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHH
AVEQVRNDGTITLLGLCTRPDTFNSFAMLSKQVKLVTSAFFTRQEYEAALDALVRGAVEP
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RLLVTDTISLDATPDVFESLRKRTHQCKVLINPGE
EEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCC
>Mature Secondary Structure
MRAAVMQGLHKPLAIDTIPDPTPGEGDVVVKVGRCGICGSDLHMTEDPAYGQGAGSVLGH
CCCHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
EFAGEVVALGKGVEGLRTGDLVSVIPLQSCGQCHSCRTGEVQWCERFGLQGGGYAEFALT
HHCCCEEEECCCCCCCCCCCEEEEECCHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEE
RPNQCVRLPASASMADGAIVEPLAVALHGLALSRMKIGDKVLVLGAGPIGLAVAFWARRF
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHHHHH
GAGRVVVQDLAEWQRDRALQMGAHDFVVDAADPVGSAGRALGGKADIVFECVGVPGLIAQ
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHH
AVEQVRNDGTITLLGLCTRPDTFNSFAMLSKQVKLVTSAFFTRQEYEAALDALVRGAVEP
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RLLVTDTISLDATPDVFESLRKRTHQCKVLINPGE
EEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]