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The map label for this gene is alkK [H]

Identifier: 87199788

GI number: 87199788

Start: 1867958

End: 1869577

Strand: Direct

Name: alkK [H]

Synonym: Saro_1771

Alternate gene names: 87199788

Gene position: 1867958-1869577 (Clockwise)

Preceding gene: 87199787

Following gene: 87199789

Centisome position: 52.45

GC content: 65.0

Gene sequence:

>1620_bases
GTGACTCAGCAGCTTGGTGCAATGCAGGACTGGAAGCTGCGCGTTACGCACCTGATCGATCATGCCGCGCGCGAGCACGG
GCAGCGCGAAATCGTCAGCCGCTGGGCAGATGGCAGCGAAAGCCGCACGACCTGGGCGGGCATCCGTCACGACGCATTGC
GCATGACGCAGGCCCTGCGCCGCCTTGGCGTGAAGCCGGGCGACCGCATCGCCACGCTTGCGATGAACCACCACCGCCAC
CTGGTAAGCTGGTACGGTTCGGTCGGCGTCGGCGGGATTCTCCATACGCTCAACCCGCGCCTGTTCGACGACCAGCTCGA
ATACATCGTCAATCACGCCGAAGACCGCGTGATGCTGTTCGACAAGCAGTGGACCCCCATCATCGAGCGCATGAAGTCGC
GCTGGCCCACGGTCGAACATTACATCTGCTTCGACAGCAGCGAGCCCGCGCTTCATTTCGAGGAGTGGATCGCTTCCGAA
GACGGGCAGACCGAATGGGCCGACGGCGATGAGCGCGACCCTTGCATGCTCTGCTACACCAGCGGCACGACCGGCAATCC
CAAGGGTGTACTGTACGAACACCGCTCGACCGTGCTCCACGCAATGAGCGCGATCAGCCCCCCGATCTTCGGGCTCGATT
GCCGTTCGGTCATGCTGCCGATCGTGCCGATGTTCCACGCGGCAAGCTGGGGCTTGCCCTGGGCGGGCGCGGCGGCGGGC
GCGAAGTTCGTATATTCCGCGGTCAATGACGGCGCGGTCCTGTGCGACCTGATGAACCGCGAGAAGGTGACCTGTTCGGC
CGGCGTGCCCACTGTCTGGCTGGCGCTGCTCCAGCATGTCGATGCCCACAATGGCGGCGAAATTCCACCGACCCTGCAGA
CCATCGTCTGCGGCGGCTCGGCCATGCCGCGCGCGATGATCGAACGCTTCATGCGCGCCGGCCGCCGCGTGGCCCACGCC
TGGGGCATGACCGAGACGTCGCCGATCGGCACTACCGGCGCGGAAACGTGGAACTGGGACGACCTCAGCTTCGACGAAAA
GGTGACCGTCAAGGCAATGCAGGGCCGTCCGCCCTTCGGCGTCGAGATCCGTTGCGTCGATCTTGGCGATCCCGGCAAGG
TGCTACCGCGCGATGGCGTGACGGCGGGGGCCTTGCAAGTGCGCGGGCCGTGGATCGTCAAGCGCTACTTCAAGGCCGAA
CAGGATGCGGTGGCCGCCGGGCAGTGGTTCGACACGGGCGATGTCGGCGTGATCCATCCGGACGGAACCCTGCAACTGAC
GGATCGCACGAAGGACGTCATCAAGTCGGGCGGCGAATGGATCAGTTCGGTCGAACTGGAGAATGCCGCCATCGGCCACC
CGGCCGTTGCCGAGGCCGCCGCGATTGGCGTCTACCATCCCAAGTGGGACGAGCGGCCGCTGCTTGTCGTGATCCGCAAG
CCGGGAATGGACGTCAACCAGGAGGAATTGCGAAGCTACCTCCAGCAGCACGTCGCCAAGTGGTGGGTGCCCGATGCGGT
GGCATTCGTGGACCAGATCCCGCATACCGGCACCGGAAAGATCAGCAAGAAGGACCTGCGCGACCAGTTCCGTGACTATC
GCTGGGACGAGGTTGCCTGA

Upstream 100 bases:

>100_bases
ACGATTGATTCCGATAGAAGGACTCGCCAATGTGGCATTGACCGCAAGACCGGAAAGGCATCTGCGGTTGGTCGAATAGA
CAGGATCGGGAGAAATTGAA

Downstream 100 bases:

>100_bases
ACGACACCTAAAGGCACCTGAAGACACCTTATCGTACACGGGAGCGCTTTCAACGCCTATGGCCGAGACCTACGTCGACC
CCAGCCGCGAGAACTTCGAG

Product: AMP-dependent synthetase and ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTQQLGAMQDWKLRVTHLIDHAAREHGQREIVSRWADGSESRTTWAGIRHDALRMTQALRRLGVKPGDRIATLAMNHHRH
LVSWYGSVGVGGILHTLNPRLFDDQLEYIVNHAEDRVMLFDKQWTPIIERMKSRWPTVEHYICFDSSEPALHFEEWIASE
DGQTEWADGDERDPCMLCYTSGTTGNPKGVLYEHRSTVLHAMSAISPPIFGLDCRSVMLPIVPMFHAASWGLPWAGAAAG
AKFVYSAVNDGAVLCDLMNREKVTCSAGVPTVWLALLQHVDAHNGGEIPPTLQTIVCGGSAMPRAMIERFMRAGRRVAHA
WGMTETSPIGTTGAETWNWDDLSFDEKVTVKAMQGRPPFGVEIRCVDLGDPGKVLPRDGVTAGALQVRGPWIVKRYFKAE
QDAVAAGQWFDTGDVGVIHPDGTLQLTDRTKDVIKSGGEWISSVELENAAIGHPAVAEAAAIGVYHPKWDERPLLVVIRK
PGMDVNQEELRSYLQQHVAKWWVPDAVAFVDQIPHTGTGKISKKDLRDQFRDYRWDEVA

Sequences:

>Translated_539_residues
MTQQLGAMQDWKLRVTHLIDHAAREHGQREIVSRWADGSESRTTWAGIRHDALRMTQALRRLGVKPGDRIATLAMNHHRH
LVSWYGSVGVGGILHTLNPRLFDDQLEYIVNHAEDRVMLFDKQWTPIIERMKSRWPTVEHYICFDSSEPALHFEEWIASE
DGQTEWADGDERDPCMLCYTSGTTGNPKGVLYEHRSTVLHAMSAISPPIFGLDCRSVMLPIVPMFHAASWGLPWAGAAAG
AKFVYSAVNDGAVLCDLMNREKVTCSAGVPTVWLALLQHVDAHNGGEIPPTLQTIVCGGSAMPRAMIERFMRAGRRVAHA
WGMTETSPIGTTGAETWNWDDLSFDEKVTVKAMQGRPPFGVEIRCVDLGDPGKVLPRDGVTAGALQVRGPWIVKRYFKAE
QDAVAAGQWFDTGDVGVIHPDGTLQLTDRTKDVIKSGGEWISSVELENAAIGHPAVAEAAAIGVYHPKWDERPLLVVIRK
PGMDVNQEELRSYLQQHVAKWWVPDAVAFVDQIPHTGTGKISKKDLRDQFRDYRWDEVA
>Mature_538_residues
TQQLGAMQDWKLRVTHLIDHAAREHGQREIVSRWADGSESRTTWAGIRHDALRMTQALRRLGVKPGDRIATLAMNHHRHL
VSWYGSVGVGGILHTLNPRLFDDQLEYIVNHAEDRVMLFDKQWTPIIERMKSRWPTVEHYICFDSSEPALHFEEWIASED
GQTEWADGDERDPCMLCYTSGTTGNPKGVLYEHRSTVLHAMSAISPPIFGLDCRSVMLPIVPMFHAASWGLPWAGAAAGA
KFVYSAVNDGAVLCDLMNREKVTCSAGVPTVWLALLQHVDAHNGGEIPPTLQTIVCGGSAMPRAMIERFMRAGRRVAHAW
GMTETSPIGTTGAETWNWDDLSFDEKVTVKAMQGRPPFGVEIRCVDLGDPGKVLPRDGVTAGALQVRGPWIVKRYFKAEQ
DAVAAGQWFDTGDVGVIHPDGTLQLTDRTKDVIKSGGEWISSVELENAAIGHPAVAEAAAIGVYHPKWDERPLLVVIRKP
GMDVNQEELRSYLQQHVAKWWVPDAVAFVDQIPHTGTGKISKKDLRDQFRDYRWDEVA

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=528, Percent_Identity=25.1893939393939, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI187761345, Length=542, Percent_Identity=26.1992619926199, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI187761343, Length=542, Percent_Identity=26.1992619926199, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI38505220, Length=490, Percent_Identity=24.6938775510204, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI28416953, Length=531, Percent_Identity=24.4821092278719, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI58082049, Length=522, Percent_Identity=24.1379310344828, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI115511026, Length=420, Percent_Identity=25.2380952380952, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI157311624, Length=526, Percent_Identity=23.9543726235741, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI157311622, Length=526, Percent_Identity=23.9543726235741, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI122937307, Length=527, Percent_Identity=24.0986717267552, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI42544132, Length=378, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI221142682, Length=532, Percent_Identity=24.4360902255639, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI145693145, Length=507, Percent_Identity=25.8382642998028, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788107, Length=506, Percent_Identity=23.9130434782609, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1786810, Length=512, Percent_Identity=25.1953125, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1790505, Length=555, Percent_Identity=22.3423423423423, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789201, Length=368, Percent_Identity=23.0978260869565, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788595, Length=363, Percent_Identity=24.7933884297521, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557194, Length=538, Percent_Identity=24.7211895910781, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=517, Percent_Identity=24.1779497098646, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=533, Percent_Identity=24.390243902439, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=523, Percent_Identity=21.2237093690249, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=491, Percent_Identity=24.847250509165, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=539, Percent_Identity=22.6345083487941, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=539, Percent_Identity=22.6345083487941, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=539, Percent_Identity=22.6345083487941, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=514, Percent_Identity=22.9571984435798, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=545, Percent_Identity=23.6697247706422, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=393, Percent_Identity=25.9541984732824, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=513, Percent_Identity=20.6627680311891, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=497, Percent_Identity=24.748490945674, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18859661, Length=522, Percent_Identity=24.5210727969349, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21355181, Length=550, Percent_Identity=22.5454545454545, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24581924, Length=553, Percent_Identity=24.9547920433996, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922652, Length=525, Percent_Identity=23.047619047619, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24656500, Length=535, Percent_Identity=22.0560747663551, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21358303, Length=518, Percent_Identity=20.4633204633205, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356947, Length=498, Percent_Identity=23.2931726907631, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648260, Length=506, Percent_Identity=22.1343873517787, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281365686, Length=509, Percent_Identity=20.2357563850688, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24653035, Length=499, Percent_Identity=20.4408817635271, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648253, Length=535, Percent_Identity=20.3738317757009, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648257, Length=539, Percent_Identity=19.2949907235622, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI20130357, Length=452, Percent_Identity=22.1238938053097, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 60081; Mature: 59950

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQLGAMQDWKLRVTHLIDHAAREHGQREIVSRWADGSESRTTWAGIRHDALRMTQALR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
RLGVKPGDRIATLAMNHHRHLVSWYGSVGVGGILHTLNPRLFDDQLEYIVNHAEDRVMLF
HHCCCCCCCEEEEHHHCCHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEE
DKQWTPIIERMKSRWPTVEHYICFDSSEPALHFEEWIASEDGQTEWADGDERDPCMLCYT
CCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
SGTTGNPKGVLYEHRSTVLHAMSAISPPIFGLDCRSVMLPIVPMFHAASWGLPWAGAAAG
CCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
AKFVYSAVNDGAVLCDLMNREKVTCSAGVPTVWLALLQHVDAHNGGEIPPTLQTIVCGGS
HHHHHHHCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHEEECCC
AMPRAMIERFMRAGRRVAHAWGMTETSPIGTTGAETWNWDDLSFDEKVTVKAMQGRPPFG
CCCHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
VEIRCVDLGDPGKVLPRDGVTAGALQVRGPWIVKRYFKAEQDAVAAGQWFDTGDVGVIHP
EEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCHHHHHCCCCCCCCCEEEECC
DGTLQLTDRTKDVIKSGGEWISSVELENAAIGHPAVAEAAAIGVYHPKWDERPLLVVIRK
CCCEEECHHHHHHHHCCCHHHHHEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEC
PGMDVNQEELRSYLQQHVAKWWVPDAVAFVDQIPHTGTGKISKKDLRDQFRDYRWDEVA
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQQLGAMQDWKLRVTHLIDHAAREHGQREIVSRWADGSESRTTWAGIRHDALRMTQALR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
RLGVKPGDRIATLAMNHHRHLVSWYGSVGVGGILHTLNPRLFDDQLEYIVNHAEDRVMLF
HHCCCCCCCEEEEHHHCCHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEE
DKQWTPIIERMKSRWPTVEHYICFDSSEPALHFEEWIASEDGQTEWADGDERDPCMLCYT
CCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
SGTTGNPKGVLYEHRSTVLHAMSAISPPIFGLDCRSVMLPIVPMFHAASWGLPWAGAAAG
CCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
AKFVYSAVNDGAVLCDLMNREKVTCSAGVPTVWLALLQHVDAHNGGEIPPTLQTIVCGGS
HHHHHHHCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHEEECCC
AMPRAMIERFMRAGRRVAHAWGMTETSPIGTTGAETWNWDDLSFDEKVTVKAMQGRPPFG
CCCHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
VEIRCVDLGDPGKVLPRDGVTAGALQVRGPWIVKRYFKAEQDAVAAGQWFDTGDVGVIHP
EEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCHHHHHCCCCCCCCCEEEECC
DGTLQLTDRTKDVIKSGGEWISSVELENAAIGHPAVAEAAAIGVYHPKWDERPLLVVIRK
CCCEEECHHHHHHHHCCCHHHHHEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEC
PGMDVNQEELRSYLQQHVAKWWVPDAVAFVDQIPHTGTGKISKKDLRDQFRDYRWDEVA
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]