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The map label for this gene is pchF [H]

Identifier: 87199669

GI number: 87199669

Start: 1727025

End: 1728656

Strand: Direct

Name: pchF [H]

Synonym: Saro_1652

Alternate gene names: 87199669

Gene position: 1727025-1728656 (Clockwise)

Preceding gene: 87199668

Following gene: 87199670

Centisome position: 48.49

GC content: 64.83

Gene sequence:

>1632_bases
ATGACTGCAGGAATCTCGCGTCGCGACATGATCCGCGCGGGCGCTGCCGGCGCCGCGCTCCTCTCCTCCCGGGCATTCGC
CAGTCCGCTCGACGGCCCGCGCATCATGCCGCCGGGCCTGGCCGCTGACCGCTTTGCCGCTGCTGTCAAGGAACTGCGGG
CCGTTGTCGGCACGGACTGGGTCTTTGCGGATGCCGAAAGCACGCTGCCCTACGCCAGCACCTTCACCCCCGACCCCGAC
GGTCGGCACCTGCCTTCGGGCGCGGTGGCCCCGGCTTCGGTCGAGGAAGTACAGGCGGTGCTGAAGGTGGCGAACAAGTA
CGGGCTGCCGCTCTGGCCGGTCTCCACCGGCAAGAACATGGGTTATGGCAATGCAACGCCTGCGACCTCGGGCCAGATGG
TGCTCGACCTCAAGCGCATGAACCGGATCATCGAGGTAGACGCCGAACTCGGTACTGCGCTGGTAGAGCCGGGCGTGACC
TACCAGGACCTCCACGACTACCTGCAGGAACACAATCTGCCCTACTGGGTCGACGTGCCCACGGTCGGGCCGATCGTGTC
GCCGCTCGGCAACACGCTGGAACGCGGGGTGGGCTATACCCCTTATGGCGACCATTTCTTCATGCAGTGCGGCATGGAAG
TCGTGCTGGCCGATGGCACGGTCGTGCGGACCGGGATGGGCAGCGTGAAGAACTCGACCACCTGGCAGGCGTTCAAGTGG
GGCTACGGCCCCTACATCGACGGCCTGTTCACCCAGTCGAACTTCGGCGTGGTGACCAAGCTCGGCATGTGGTTGATGCC
GGCGCCGCCAGCCTACAAGCCCTTCATGGTCCGTCACATGGAAGTGGCCGACGTGGCGCGGATCGTCGATGCGATCCGCC
CGTTCCGCATGAACAACCTCATCCCCAATTGCGTCTTGATGATGGGCGCGGCCTACCAGCTCGCGATGTTCAAGCGCCGC
GCCGACATCTGGACCGAGCAGCGCTCCGTTCCGGATGACGTGATCCGGGCCGAGGCTATGCGGAACGGCCTCGGCATGTG
GAACACCTATTTCGCGCTCTACGGTACCGATGAGATCATCGCTGCGGTGGAACCCATCGTTCGCTCCGCCTTCGAGGCGA
CCGGCGGCGAGGTACTGACCGAGAGGGAAATGTCCGGCAACCCGTGGTTCGAACATCACAAGTCGCTGATGCGTGGCGGC
ATGACGTTGGAGGAGATCGGCATCGTGCGCTGGCGCGGGCCCGGTGGCGGGATGATCTGCTTTGCCCCGGTCGCTCCGGC
CAAGGGCGTCGAGACCGCCGAGCAGACCGCGCTCGCCAAGGAAATCCTCGGCAAGTACGACTTCGACTACAACGGTGCCT
TCGCCATCGGCAGCCGCGAACTGCACCACCTGATCTTCCTGCTGTTCGACAAGGATGATCCGGCCGAGGAACGCAAGGCG
CAGGACTGCATGGAAGAGATGATCCTGCGCTTCGGCGACAAGGGCTGGGCCGCGTATCGCACCGCCGTCAGCACCATGGA
TCTCGTAGCAGGCCAGTACGGCGAGGCGAATAGGATGCTCAATCGGCGCCTGAAGGCGGCGCTCGACCCAAACGGTGTCA
TCGCGCCCGGAAAATCGGGGATCACGCTTTGA

Upstream 100 bases:

>100_bases
CGATGCCCGCCTTCCGCCCGACCGAGATCACCGCCGCCGAACTCGCTGCCCTTGGCGTTTGGATCGAAAAGTCCCGGGCC
AATCCGAAGGAGCATGGACA

Downstream 100 bases:

>100_bases
CGATGCGATGCGTCGACCCGTCGCAAGTCTCTTGTCTATCAAATTAGTTGAGTTATCTCCTGAAGCCTGTCGGGACGTAA
TCCGAGTCGACCGGCTTTCC

Product: twin-arginine translocation pathway signal protein

Products: NA

Alternate protein names: P-cresol methylhydroxylase; PCMH [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MTAGISRRDMIRAGAAGAALLSSRAFASPLDGPRIMPPGLAADRFAAAVKELRAVVGTDWVFADAESTLPYASTFTPDPD
GRHLPSGAVAPASVEEVQAVLKVANKYGLPLWPVSTGKNMGYGNATPATSGQMVLDLKRMNRIIEVDAELGTALVEPGVT
YQDLHDYLQEHNLPYWVDVPTVGPIVSPLGNTLERGVGYTPYGDHFFMQCGMEVVLADGTVVRTGMGSVKNSTTWQAFKW
GYGPYIDGLFTQSNFGVVTKLGMWLMPAPPAYKPFMVRHMEVADVARIVDAIRPFRMNNLIPNCVLMMGAAYQLAMFKRR
ADIWTEQRSVPDDVIRAEAMRNGLGMWNTYFALYGTDEIIAAVEPIVRSAFEATGGEVLTEREMSGNPWFEHHKSLMRGG
MTLEEIGIVRWRGPGGGMICFAPVAPAKGVETAEQTALAKEILGKYDFDYNGAFAIGSRELHHLIFLLFDKDDPAEERKA
QDCMEEMILRFGDKGWAAYRTAVSTMDLVAGQYGEANRMLNRRLKAALDPNGVIAPGKSGITL

Sequences:

>Translated_543_residues
MTAGISRRDMIRAGAAGAALLSSRAFASPLDGPRIMPPGLAADRFAAAVKELRAVVGTDWVFADAESTLPYASTFTPDPD
GRHLPSGAVAPASVEEVQAVLKVANKYGLPLWPVSTGKNMGYGNATPATSGQMVLDLKRMNRIIEVDAELGTALVEPGVT
YQDLHDYLQEHNLPYWVDVPTVGPIVSPLGNTLERGVGYTPYGDHFFMQCGMEVVLADGTVVRTGMGSVKNSTTWQAFKW
GYGPYIDGLFTQSNFGVVTKLGMWLMPAPPAYKPFMVRHMEVADVARIVDAIRPFRMNNLIPNCVLMMGAAYQLAMFKRR
ADIWTEQRSVPDDVIRAEAMRNGLGMWNTYFALYGTDEIIAAVEPIVRSAFEATGGEVLTEREMSGNPWFEHHKSLMRGG
MTLEEIGIVRWRGPGGGMICFAPVAPAKGVETAEQTALAKEILGKYDFDYNGAFAIGSRELHHLIFLLFDKDDPAEERKA
QDCMEEMILRFGDKGWAAYRTAVSTMDLVAGQYGEANRMLNRRLKAALDPNGVIAPGKSGITL
>Mature_542_residues
TAGISRRDMIRAGAAGAALLSSRAFASPLDGPRIMPPGLAADRFAAAVKELRAVVGTDWVFADAESTLPYASTFTPDPDG
RHLPSGAVAPASVEEVQAVLKVANKYGLPLWPVSTGKNMGYGNATPATSGQMVLDLKRMNRIIEVDAELGTALVEPGVTY
QDLHDYLQEHNLPYWVDVPTVGPIVSPLGNTLERGVGYTPYGDHFFMQCGMEVVLADGTVVRTGMGSVKNSTTWQAFKWG
YGPYIDGLFTQSNFGVVTKLGMWLMPAPPAYKPFMVRHMEVADVARIVDAIRPFRMNNLIPNCVLMMGAAYQLAMFKRRA
DIWTEQRSVPDDVIRAEAMRNGLGMWNTYFALYGTDEIIAAVEPIVRSAFEATGGEVLTEREMSGNPWFEHHKSLMRGGM
TLEEIGIVRWRGPGGGMICFAPVAPAKGVETAEQTALAKEILGKYDFDYNGAFAIGSRELHHLIFLLFDKDDPAEERKAQ
DCMEEMILRFGDKGWAAYRTAVSTMDLVAGQYGEANRMLNRRLKAALDPNGVIAPGKSGITL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=229, Percent_Identity=29.6943231441048, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI37595754, Length=245, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI48994907, Length=187, Percent_Identity=32.620320855615, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320027, Length=269, Percent_Identity=27.8810408921933, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR006094
- InterPro:   IPR016170
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: =1.17.99.1 [H]

Molecular weight: Translated: 59317; Mature: 59186

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGISRRDMIRAGAAGAALLSSRAFASPLDGPRIMPPGLAADRFAAAVKELRAVVGTDW
CCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
VFADAESTLPYASTFTPDPDGRHLPSGAVAPASVEEVQAVLKVANKYGLPLWPVSTGKNM
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
GYGNATPATSGQMVLDLKRMNRIIEVDAELGTALVEPGVTYQDLHDYLQEHNLPYWVDVP
CCCCCCCCCCCCEEEEHHHHHHHEEECHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECC
TVGPIVSPLGNTLERGVGYTPYGDHFFMQCGMEVVLADGTVVRTGMGSVKNSTTWQAFKW
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCCEEC
GYGPYIDGLFTQSNFGVVTKLGMWLMPAPPAYKPFMVRHMEVADVARIVDAIRPFRMNNL
CCCCHHCCEEECCCCCHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCC
IPNCVLMMGAAYQLAMFKRRADIWTEQRSVPDDVIRAEAMRNGLGMWNTYFALYGTDEII
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHEEEEECCHHHH
AAVEPIVRSAFEATGGEVLTEREMSGNPWFEHHKSLMRGGMTLEEIGIVRWRGPGGGMIC
HHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCHHHCCEEEEECCCCCEEE
FAPVAPAKGVETAEQTALAKEILGKYDFDYNGAFAIGSRELHHLIFLLFDKDDPAEERKA
EECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCHHCEEEEEEEECCCCCHHHHHH
QDCMEEMILRFGDKGWAAYRTAVSTMDLVAGQYGEANRMLNRRLKAALDPNGVIAPGKSG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCC
ITL
CCC
>Mature Secondary Structure 
TAGISRRDMIRAGAAGAALLSSRAFASPLDGPRIMPPGLAADRFAAAVKELRAVVGTDW
CCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
VFADAESTLPYASTFTPDPDGRHLPSGAVAPASVEEVQAVLKVANKYGLPLWPVSTGKNM
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
GYGNATPATSGQMVLDLKRMNRIIEVDAELGTALVEPGVTYQDLHDYLQEHNLPYWVDVP
CCCCCCCCCCCCEEEEHHHHHHHEEECHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECC
TVGPIVSPLGNTLERGVGYTPYGDHFFMQCGMEVVLADGTVVRTGMGSVKNSTTWQAFKW
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCCEEC
GYGPYIDGLFTQSNFGVVTKLGMWLMPAPPAYKPFMVRHMEVADVARIVDAIRPFRMNNL
CCCCHHCCEEECCCCCHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCC
IPNCVLMMGAAYQLAMFKRRADIWTEQRSVPDDVIRAEAMRNGLGMWNTYFALYGTDEII
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHEEEEECCHHHH
AAVEPIVRSAFEATGGEVLTEREMSGNPWFEHHKSLMRGGMTLEEIGIVRWRGPGGGMIC
HHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCHHHCCEEEEECCCCCEEE
FAPVAPAKGVETAEQTALAKEILGKYDFDYNGAFAIGSRELHHLIFLLFDKDDPAEERKA
EECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCHHCEEEEEEEECCCCCHHHHHH
QDCMEEMILRFGDKGWAAYRTAVSTMDLVAGQYGEANRMLNRRLKAALDPNGVIAPGKSG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCC
ITL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929007; 10565539; 3790500; 1846290; 10623531 [H]