Click here to switch to the map view.

The map label for this gene is pchF [H]

Identifier: 87199511

GI number: 87199511

Start: 1534481

End: 1536040

Strand: Reverse

Name: pchF [H]

Synonym: Saro_1492

Alternate gene names: 87199511

Gene position: 1536040-1534481 (Counterclockwise)

Preceding gene: 87199512

Following gene: 87199510

Centisome position: 43.13

GC content: 60.51

Gene sequence:

>1560_bases
ATGAGCGCGCTACTTGCCCAAGGCGTTTCCCCCGCGCAATTTGCACAAGCGCTGGATGCGATGCGCGCTGTCGTTGGCCC
ACAGTGGCTTTTTGCCAGCGAAGAAGACATCGCGGCCTACAGTGATCACTTCGCCTTCGAAGACGTGACTGCCAACATGG
CATCGGCGATCGTCGCCCCGCTAGGCCTCGACCAGATCACCAGGATCATCGGCATTGCACGCGATCACCGGATCCCGGTC
TGGGCGATTTCGACCGGTCGCAACCTTGCCTACGGCGGATCCGCGCCGCGTAAGCACGGCACGCTGACGCTGGATCTCAA
GCGCAACAACCGCATCCTCGAGGTTAACGAAGAGTTGGCCTACGCGGTGGTCGAGCCGGGGGTATCGTTCTTCCAACTGT
ATCGCCACTTGCGCGAAACTGGCTCCAAGCTCTGGATCGACACGCCATCGCCAGGCTGGGGCGGTATTATGGGCAATATG
CTCGAACGCGGGGTCGGCTACACGCCCTACGGCGACCGCTTCATGTGGCAATGCGGGATGCAGGTGGTGCTGGCCGACGG
TACGGTTGTGGACACCGGCATGGCCGCGCAGGAAGGTGCGCCCGGCAATCACACGTATCGTTATGGCGGCGGCCCGTGGA
TCGACGGAATTTTCACGCAGTCCAATTTCGGCATCGTCACCAAGGTCGGCATCCAGTTGATGCCTGAACCACCGGGATAT
CGCCCGTTCCTTGTCACCTTTGCTGAAGATGATGACATCGAGCCGGTCTCAGACCTGATCCGCCCGCTCAAGATGACGCA
CATCATCCCCAACGCGGCCGTCACCTGCAGCCTCAACCTGGAAGCAGCCACATCGCTGGACCGGACGAAGTACCATTCAG
ATTCTGGCCCCGTGCCAGAAGCGGGCCGCCGCCGGATGATGGAGGATCTGGGCGTCGGCAAATGGAACTTTTACGCCGCC
CTCTATGGCCCTGAACCGGTCATGGATGCCCATTGGGAAGTAATCCGCGACAGCTTCTCTTCGGTGAAGGGCGCGCGCTT
CTTCACCGAAGAAGACCGCAAAAACGATGTCGTGTTCGGATATCGCACCCAGTTGATGCGCGGAGAACCGAACATGACCG
AGTTCGGTATCCTCAACTGGATGCCGAATGGTGCCCACCTCGGTTTTTCGCCTGTGGCCCCGGTCGACGGCAAAACCGCG
CTCGACCAGTACCGCCTAGCCGAAGCAATCTGTAACCGGCATGGCTTCGACTATACCGGCATGTTCATCGTCGGCTTCCG
CGCGATGCACCACATCGTCGAACCGATCTTCTCGCGTAGCGATGAGGATCAGCGCGGTCGAGTGGTCAGCATGGTCACCG
AACTGATCGATGAGGCCGCCAAGCGTGGCTACGGCGAATATCGCGGTCACCTCAGCTTCATGGATCAGATCGCCGGTACT
TATGGCTGGGGCGACGACGCGCTCATGAAGCTTAGCCAACGCATCAAGCGCGCACTGGACCCTTCGGGCATCATGGCCCC
CGGGAAGAGTGGCATCTGGTCGGATGGAGCGTCCTCATGA

Upstream 100 bases:

>100_bases
GTCCGTCAGTGCCCCGCTTCCATTCGTCCCTGGAACTTGAAACGCCTGCCCATGCCGGTCCGGCGTTCCTCAACAGCTTC
CTGTTCGAACGGCTTTCGGC

Downstream 100 bases:

>100_bases
AAACACTGTTCTGCATTTTCGCATCCCTGCCACTGGCCGGCTGTTCCGTTGCATCGGCTGCCCCTGACGGCCGGACTCTG
TTCGCGCAAAATTGTTCATC

Product: vanillyl-alcohol oxidase

Products: NA

Alternate protein names: P-cresol methylhydroxylase; PCMH [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSALLAQGVSPAQFAQALDAMRAVVGPQWLFASEEDIAAYSDHFAFEDVTANMASAIVAPLGLDQITRIIGIARDHRIPV
WAISTGRNLAYGGSAPRKHGTLTLDLKRNNRILEVNEELAYAVVEPGVSFFQLYRHLRETGSKLWIDTPSPGWGGIMGNM
LERGVGYTPYGDRFMWQCGMQVVLADGTVVDTGMAAQEGAPGNHTYRYGGGPWIDGIFTQSNFGIVTKVGIQLMPEPPGY
RPFLVTFAEDDDIEPVSDLIRPLKMTHIIPNAAVTCSLNLEAATSLDRTKYHSDSGPVPEAGRRRMMEDLGVGKWNFYAA
LYGPEPVMDAHWEVIRDSFSSVKGARFFTEEDRKNDVVFGYRTQLMRGEPNMTEFGILNWMPNGAHLGFSPVAPVDGKTA
LDQYRLAEAICNRHGFDYTGMFIVGFRAMHHIVEPIFSRSDEDQRGRVVSMVTELIDEAAKRGYGEYRGHLSFMDQIAGT
YGWGDDALMKLSQRIKRALDPSGIMAPGKSGIWSDGASS

Sequences:

>Translated_519_residues
MSALLAQGVSPAQFAQALDAMRAVVGPQWLFASEEDIAAYSDHFAFEDVTANMASAIVAPLGLDQITRIIGIARDHRIPV
WAISTGRNLAYGGSAPRKHGTLTLDLKRNNRILEVNEELAYAVVEPGVSFFQLYRHLRETGSKLWIDTPSPGWGGIMGNM
LERGVGYTPYGDRFMWQCGMQVVLADGTVVDTGMAAQEGAPGNHTYRYGGGPWIDGIFTQSNFGIVTKVGIQLMPEPPGY
RPFLVTFAEDDDIEPVSDLIRPLKMTHIIPNAAVTCSLNLEAATSLDRTKYHSDSGPVPEAGRRRMMEDLGVGKWNFYAA
LYGPEPVMDAHWEVIRDSFSSVKGARFFTEEDRKNDVVFGYRTQLMRGEPNMTEFGILNWMPNGAHLGFSPVAPVDGKTA
LDQYRLAEAICNRHGFDYTGMFIVGFRAMHHIVEPIFSRSDEDQRGRVVSMVTELIDEAAKRGYGEYRGHLSFMDQIAGT
YGWGDDALMKLSQRIKRALDPSGIMAPGKSGIWSDGASS
>Mature_518_residues
SALLAQGVSPAQFAQALDAMRAVVGPQWLFASEEDIAAYSDHFAFEDVTANMASAIVAPLGLDQITRIIGIARDHRIPVW
AISTGRNLAYGGSAPRKHGTLTLDLKRNNRILEVNEELAYAVVEPGVSFFQLYRHLRETGSKLWIDTPSPGWGGIMGNML
ERGVGYTPYGDRFMWQCGMQVVLADGTVVDTGMAAQEGAPGNHTYRYGGGPWIDGIFTQSNFGIVTKVGIQLMPEPPGYR
PFLVTFAEDDDIEPVSDLIRPLKMTHIIPNAAVTCSLNLEAATSLDRTKYHSDSGPVPEAGRRRMMEDLGVGKWNFYAAL
YGPEPVMDAHWEVIRDSFSSVKGARFFTEEDRKNDVVFGYRTQLMRGEPNMTEFGILNWMPNGAHLGFSPVAPVDGKTAL
DQYRLAEAICNRHGFDYTGMFIVGFRAMHHIVEPIFSRSDEDQRGRVVSMVTELIDEAAKRGYGEYRGHLSFMDQIAGTY
GWGDDALMKLSQRIKRALDPSGIMAPGKSGIWSDGASS

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=228, Percent_Identity=28.0701754385965, Blast_Score=70, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR006094
- InterPro:   IPR016170
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: =1.17.99.1 [H]

Molecular weight: Translated: 57222; Mature: 57090

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLAQGVSPAQFAQALDAMRAVVGPQWLFASEEDIAAYSDHFAFEDVTANMASAIVAP
CCCHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCCHHHHHHHHHHHHHHHC
LGLDQITRIIGIARDHRIPVWAISTGRNLAYGGSAPRKHGTLTLDLKRNNRILEVNEELA
CCHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCEEEEEECCCCEEEECHHHH
YAVVEPGVSFFQLYRHLRETGSKLWIDTPSPGWGGIMGNMLERGVGYTPYGDRFMWQCGM
HHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHCCCC
QVVLADGTVVDTGMAAQEGAPGNHTYRYGGGPWIDGIFTQSNFGIVTKVGIQLMPEPPGY
EEEEECCEEEECCCCCCCCCCCCCCEECCCCCEECCEEECCCCCEEEEECEEECCCCCCC
RPFLVTFAEDDDIEPVSDLIRPLKMTHIIPNAAVTCSLNLEAATSLDRTKYHSDSGPVPE
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCHHHHCCCCCCCCCH
AGRRRMMEDLGVGKWNFYAALYGPEPVMDAHWEVIRDSFSSVKGARFFTEEDRKNDVVFG
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEE
YRTQLMRGEPNMTEFGILNWMPNGAHLGFSPVAPVDGKTALDQYRLAEAICNRHGFDYTG
EHHHHHCCCCCCCCEEHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCH
MFIVGFRAMHHIVEPIFSRSDEDQRGRVVSMVTELIDEAAKRGYGEYRGHLSFMDQIAGT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
YGWGDDALMKLSQRIKRALDPSGIMAPGKSGIWSDGASS
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SALLAQGVSPAQFAQALDAMRAVVGPQWLFASEEDIAAYSDHFAFEDVTANMASAIVAP
CCHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCCHHHHHHHHHHHHHHHC
LGLDQITRIIGIARDHRIPVWAISTGRNLAYGGSAPRKHGTLTLDLKRNNRILEVNEELA
CCHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCEEEEEECCCCEEEECHHHH
YAVVEPGVSFFQLYRHLRETGSKLWIDTPSPGWGGIMGNMLERGVGYTPYGDRFMWQCGM
HHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHCCCC
QVVLADGTVVDTGMAAQEGAPGNHTYRYGGGPWIDGIFTQSNFGIVTKVGIQLMPEPPGY
EEEEECCEEEECCCCCCCCCCCCCCEECCCCCEECCEEECCCCCEEEEECEEECCCCCCC
RPFLVTFAEDDDIEPVSDLIRPLKMTHIIPNAAVTCSLNLEAATSLDRTKYHSDSGPVPE
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCHHHHCCCCCCCCCH
AGRRRMMEDLGVGKWNFYAALYGPEPVMDAHWEVIRDSFSSVKGARFFTEEDRKNDVVFG
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEE
YRTQLMRGEPNMTEFGILNWMPNGAHLGFSPVAPVDGKTALDQYRLAEAICNRHGFDYTG
EHHHHHCCCCCCCCEEHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCH
MFIVGFRAMHHIVEPIFSRSDEDQRGRVVSMVTELIDEAAKRGYGEYRGHLSFMDQIAGT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
YGWGDDALMKLSQRIKRALDPSGIMAPGKSGIWSDGASS
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929007; 10565539; 3790500; 1846290; 10623531 [H]