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The map label for this gene is dnaN [H]

Identifier: 87199450

GI number: 87199450

Start: 1470682

End: 1471809

Strand: Direct

Name: dnaN [H]

Synonym: Saro_1429

Alternate gene names: 87199450

Gene position: 1470682-1471809 (Clockwise)

Preceding gene: 87199449

Following gene: 87199451

Centisome position: 41.29

GC content: 62.77

Gene sequence:

>1128_bases
ATGAAGGCGACGATCGAACGCGCTACGCTGCTGCGCTGCTTGTCCCACGTCCAGTCGGTGGTCGAACGCCGCAACACCAT
CCCCATCCTGTCGAACGTGCTCATCGAAGCCGCGCCCGACAGCACGGTGCGCCTGATGGCAACCGACCTCGACCTGCAGA
TCATCGAAACGATGAGCGCGGTCTCGGTTGAAGCGCCAGGCGCCATCACGGTTTCTGCGCACCTCCTGTTCGACATCGCG
CGCAAGCTGCCCGACGGTTCGCAGGTCAGCCTGGAGACGTCGGACAACCGCATGATCGTGAAGGCTGGCCGCAGCCGCTT
TCAGTTGCCGACCCTGCCGCGCGACGATTTCCCCGTGATCGTCGAAGGCGATCTCCCGACCAGCTTCGAGATCCCGGCGC
GCACGCTCGCCGAGTTGATCGATCGCACGCGCTTCGCGATCTCGACCGAGGAAACGCGCTACTACCTCAACGGTATCTTC
CTGCACGTTTCCGACGATCCGCAGCCGGTGCTGAAAGCCGCCGCGACCGACGGCCACCGTCTTGCCCGTTTCACCATCAC
CCGCCCGGATGGAGCGGAAGGCATGCCGGACGTGATCGTGCCGAGGAAGTGCGTGGGCGAACTGCGCAAGCTGCTCGAAG
AAGTGCTCGACAGCGCGGTGCTGATCGACCTTTCCGCCAGCAAGATCCGCTTTACCTTGGGTGGCGAAAACGGCGTGGTC
CTTACGAGCAAGCTGATCGACGGGACGTTCCCCGACTACAGCCGCGTCATTCCGACCGGAAACGACAAGCTTCTGAAGCT
CGATCCGCGCTCTTTCTTCGAAGGCGTGGATCGCGTGGCGACCATCGCCACGGAAAAGACCCGGGCGGTCAAGATGGCGC
TGGAGAACGACCGTGTAACGCTGTCGGTAACGAGCCCGGACAATGGCACGGCGGCCGAAGAGCTGCCGGCAGACTACAGC
TCGACCGGCTTCGAGATCGGCTTCAATGCCAACTATCTCAAGGACATTCTCAGCCAGATCGACGGTGACACCGTGGAACT
GCATTTGGCCGATGCGGGCGCGCCGACGCTGATCCGCAAGGACGACAAGTCGGCCGCGCTTTACGTTCTCATGCCAATGC
GCGTTTGA

Upstream 100 bases:

>100_bases
TAAGACAGATGGTCCGAACCGTACGCCGGGGACGCGATTCCTCGGCCCAGTCAATGCAAGGAAAGGGCGAAGGCCCGCTC
CTCGGGGGATCGAGACGAGA

Downstream 100 bases:

>100_bases
GGGGGCTGGTCCCGGGGAAGCAAACCTTTCCCGGGACTGGTGAACTGCGATTGCAATCGATGGATGGTTAACGGTGTGTT
CGGCTGTCCTCCGGTAAATC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKATIERATLLRCLSHVQSVVERRNTIPILSNVLIEAAPDSTVRLMATDLDLQIIETMSAVSVEAPGAITVSAHLLFDIA
RKLPDGSQVSLETSDNRMIVKAGRSRFQLPTLPRDDFPVIVEGDLPTSFEIPARTLAELIDRTRFAISTEETRYYLNGIF
LHVSDDPQPVLKAAATDGHRLARFTITRPDGAEGMPDVIVPRKCVGELRKLLEEVLDSAVLIDLSASKIRFTLGGENGVV
LTSKLIDGTFPDYSRVIPTGNDKLLKLDPRSFFEGVDRVATIATEKTRAVKMALENDRVTLSVTSPDNGTAAEELPADYS
STGFEIGFNANYLKDILSQIDGDTVELHLADAGAPTLIRKDDKSAALYVLMPMRV

Sequences:

>Translated_375_residues
MKATIERATLLRCLSHVQSVVERRNTIPILSNVLIEAAPDSTVRLMATDLDLQIIETMSAVSVEAPGAITVSAHLLFDIA
RKLPDGSQVSLETSDNRMIVKAGRSRFQLPTLPRDDFPVIVEGDLPTSFEIPARTLAELIDRTRFAISTEETRYYLNGIF
LHVSDDPQPVLKAAATDGHRLARFTITRPDGAEGMPDVIVPRKCVGELRKLLEEVLDSAVLIDLSASKIRFTLGGENGVV
LTSKLIDGTFPDYSRVIPTGNDKLLKLDPRSFFEGVDRVATIATEKTRAVKMALENDRVTLSVTSPDNGTAAEELPADYS
STGFEIGFNANYLKDILSQIDGDTVELHLADAGAPTLIRKDDKSAALYVLMPMRV
>Mature_375_residues
MKATIERATLLRCLSHVQSVVERRNTIPILSNVLIEAAPDSTVRLMATDLDLQIIETMSAVSVEAPGAITVSAHLLFDIA
RKLPDGSQVSLETSDNRMIVKAGRSRFQLPTLPRDDFPVIVEGDLPTSFEIPARTLAELIDRTRFAISTEETRYYLNGIF
LHVSDDPQPVLKAAATDGHRLARFTITRPDGAEGMPDVIVPRKCVGELRKLLEEVLDSAVLIDLSASKIRFTLGGENGVV
LTSKLIDGTFPDYSRVIPTGNDKLLKLDPRSFFEGVDRVATIATEKTRAVKMALENDRVTLSVTSPDNGTAAEELPADYS
STGFEIGFNANYLKDILSQIDGDTVELHLADAGAPTLIRKDDKSAALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=377, Percent_Identity=36.604774535809, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41002; Mature: 41002

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATIERATLLRCLSHVQSVVERRNTIPILSNVLIEAAPDSTVRLMATDLDLQIIETMSA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCCCCEEEEEEECCCHHHHHHHHH
VSVEAPGAITVSAHLLFDIARKLPDGSQVSLETSDNRMIVKAGRSRFQLPTLPRDDFPVI
HEECCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCEE
VEGDLPTSFEIPARTLAELIDRTRFAISTEETRYYLNGIFLHVSDDPQPVLKAAATDGHR
EECCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCHHHHHHHCCCCCE
LARFTITRPDGAEGMPDVIVPRKCVGELRKLLEEVLDSAVLIDLSASKIRFTLGGENGVV
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCEE
LTSKLIDGTFPDYSRVIPTGNDKLLKLDPRSFFEGVDRVATIATEKTRAVKMALENDRVT
EEEEHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCHHHEEEEEECCCEEE
LSVTSPDNGTAAEELPADYSSTGFEIGFNANYLKDILSQIDGDTVELHLADAGAPTLIRK
EEEECCCCCCCHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEE
DDKSAALYVLMPMRV
CCCCEEEEEEEECCC
>Mature Secondary Structure
MKATIERATLLRCLSHVQSVVERRNTIPILSNVLIEAAPDSTVRLMATDLDLQIIETMSA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCCCCEEEEEEECCCHHHHHHHHH
VSVEAPGAITVSAHLLFDIARKLPDGSQVSLETSDNRMIVKAGRSRFQLPTLPRDDFPVI
HEECCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCEE
VEGDLPTSFEIPARTLAELIDRTRFAISTEETRYYLNGIFLHVSDDPQPVLKAAATDGHR
EECCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCHHHHHHHCCCCCE
LARFTITRPDGAEGMPDVIVPRKCVGELRKLLEEVLDSAVLIDLSASKIRFTLGGENGVV
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCEE
LTSKLIDGTFPDYSRVIPTGNDKLLKLDPRSFFEGVDRVATIATEKTRAVKMALENDRVT
EEEEHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCHHHEEEEEECCCEEE
LSVTSPDNGTAAEELPADYSSTGFEIGFNANYLKDILSQIDGDTVELHLADAGAPTLIRK
EEEECCCCCCCHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEE
DDKSAALYVLMPMRV
CCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]