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The map label for this gene is purB [H]

Identifier: 87199330

GI number: 87199330

Start: 1352436

End: 1353752

Strand: Reverse

Name: purB [H]

Synonym: Saro_1309

Alternate gene names: 87199330

Gene position: 1353752-1352436 (Counterclockwise)

Preceding gene: 87199331

Following gene: 87199329

Centisome position: 38.01

GC content: 66.59

Gene sequence:

>1317_bases
ATGGTCCCCCGCTACGCCCGCCCCGCCATGACCGCGATCTGGGAGCCCGCAGCCCGCTATCGCATCTGGTTCGAGATCGA
GGCCTATGCGACCGAGAAGCTCGGCGAACTCGGCGTCGTCCCGCCCAGCGGCGCGAAGGCGCTGTGGGACTGGTGGGCGA
CCAACCCCGCCATCGATGTCGCCGCGATCGACGCCATCGAGGCCGTGACCAAGCACGACGTCATCGCCTTCCTCGACTGG
GTGGCCCAGCAGGTCGGCCCCGAAGCGCGCTTCATGCACCAGGGCATGACCAGCTCCGACGTGCTCGACACCACGCTCAG
CGTCCAGCTCGCCCGCGCTTCGGACATCCTGCTGGCCGATCTTGACGCCCTGCTCGAAGCGATCAAGCGCCGCGCGTTCG
AACACAAGTACACGCCGACCATCGGGCGCAGCCACGGCATCCACGCCGAACCGACGACCTTCGGCAAGAAGCTCGCCGAA
GCCTATGCCGAGTTCACCCGTTGCCGCGCCCGCCTTGTCGCGGCGCGCGCCGAAGTGGCGACCTGCGCGATCTCGGGCGC
GGTCGGCACCTTCGCCAACATCGATCCTTCGGTCGAGGAATACGTCGCCGAGAAGCTCGGCCTGGCGGTCGAACCGGTAT
CGACCCAGGTCATCCCGCGTGATCGCCACGCGATGTTCTTCGCGACGCTCGGCGTGATCGCCAGCTCGATCGAACGCCTC
GCCGTCGAGGTTCGCCACCTCCAGCGCACCGAAGTGCTCGAGGCCGAGGAATACTTCTCGCCCGGCCAGAAGGGCTCGTC
GGCCATGCCGCACAAGCGCAACCCGGTGCTGACCGAAAACCTCACCGGCCTTGCCCGCATGGTCCGCTCCGCCGTGACCC
CCGCGCTGGAAAACGTGGCGCTGTGGCACGAGCGCGACATCTCTCACTCGTCGGTCGAACGCTTCATCGGGCCCGATGCC
ACGATCACGCTCGACTTCGCTCTCGCGCGGCTGACCTCGGTGGTCGACAAGCTCGTCGTCTATCCCGAGCGGATGCAGAA
GAACCTCGACAAGATGGGCGGCCTCGTCCACTCGCAGCGCGTTCTCCTGGCCCTTACCCAGGCCGGTCTGGAACGCGACA
CCGCCTACCGCCTGGTCCAGCGCAACGCGATGAAGGTCTGGGAATCGGACGGCCAGCTCAACCTCATGGAACTGCTCAAG
GCCGATCCCGAAGTCGCCGCCGCGCTGACGAAGGAACAGATCGAGGAGAAGTTCAACCTCGACTACCACTTCAAGGCGAT
CGACACGATCTTCGACCGCGTCTTCGGCCAGGCCTGA

Upstream 100 bases:

>100_bases
TTCATTCGGACCTGCCTTTTCCTGAACGGCATGGCCTCGCCCCTTGCGTTTGAGCGGCCTAACCCCTAGCGGCCCGGGCA
AGATTTTCACGGAGTCGAAC

Downstream 100 bases:

>100_bases
AACGGACCGGGCGCGCAAAGCGGGGGGACTCGCCAAGCGCGCTGGCCTGCCCTAGCATTGCGGCGAAACGGGCGGGTCAC
GGAGCGGAAGGCATGAGGAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVPRYARPAMTAIWEPAARYRIWFEIEAYATEKLGELGVVPPSGAKALWDWWATNPAIDVAAIDAIEAVTKHDVIAFLDW
VAQQVGPEARFMHQGMTSSDVLDTTLSVQLARASDILLADLDALLEAIKRRAFEHKYTPTIGRSHGIHAEPTTFGKKLAE
AYAEFTRCRARLVAARAEVATCAISGAVGTFANIDPSVEEYVAEKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSIERL
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPALENVALWHERDISHSSVERFIGPDA
TITLDFALARLTSVVDKLVVYPERMQKNLDKMGGLVHSQRVLLALTQAGLERDTAYRLVQRNAMKVWESDGQLNLMELLK
ADPEVAAALTKEQIEEKFNLDYHFKAIDTIFDRVFGQA

Sequences:

>Translated_438_residues
MVPRYARPAMTAIWEPAARYRIWFEIEAYATEKLGELGVVPPSGAKALWDWWATNPAIDVAAIDAIEAVTKHDVIAFLDW
VAQQVGPEARFMHQGMTSSDVLDTTLSVQLARASDILLADLDALLEAIKRRAFEHKYTPTIGRSHGIHAEPTTFGKKLAE
AYAEFTRCRARLVAARAEVATCAISGAVGTFANIDPSVEEYVAEKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSIERL
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPALENVALWHERDISHSSVERFIGPDA
TITLDFALARLTSVVDKLVVYPERMQKNLDKMGGLVHSQRVLLALTQAGLERDTAYRLVQRNAMKVWESDGQLNLMELLK
ADPEVAAALTKEQIEEKFNLDYHFKAIDTIFDRVFGQA
>Mature_438_residues
MVPRYARPAMTAIWEPAARYRIWFEIEAYATEKLGELGVVPPSGAKALWDWWATNPAIDVAAIDAIEAVTKHDVIAFLDW
VAQQVGPEARFMHQGMTSSDVLDTTLSVQLARASDILLADLDALLEAIKRRAFEHKYTPTIGRSHGIHAEPTTFGKKLAE
AYAEFTRCRARLVAARAEVATCAISGAVGTFANIDPSVEEYVAEKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSIERL
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPALENVALWHERDISHSSVERFIGPDA
TITLDFALARLTSVVDKLVVYPERMQKNLDKMGGLVHSQRVLLALTQAGLERDTAYRLVQRNAMKVWESDGQLNLMELLK
ADPEVAAALTKEQIEEKFNLDYHFKAIDTIFDRVFGQA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=418, Percent_Identity=26.555023923445, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=26.8229166666667, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1787376, Length=354, Percent_Identity=29.0960451977401, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=23.989898989899, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32564234, Length=366, Percent_Identity=24.8633879781421, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323391, Length=418, Percent_Identity=27.7511961722488, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24647570, Length=416, Percent_Identity=25.9615384615385, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48617; Mature: 48617

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPRYARPAMTAIWEPAARYRIWFEIEAYATEKLGELGVVPPSGAKALWDWWATNPAIDV
CCCCCCCCHHHHHHCCHHHEEEEEEEEHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHH
AAIDAIEAVTKHDVIAFLDWVAQQVGPEARFMHQGMTSSDVLDTTLSVQLARASDILLAD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDALLEAIKRRAFEHKYTPTIGRSHGIHAEPTTFGKKLAEAYAEFTRCRARLVAARAEVA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCAISGAVGTFANIDPSVEEYVAEKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSIERL
HHHHHCCCCCCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPALENVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LWHERDISHSSVERFIGPDATITLDFALARLTSVVDKLVVYPERMQKNLDKMGGLVHSQR
HHHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
VLLALTQAGLERDTAYRLVQRNAMKVWESDGQLNLMELLKADPEVAAALTKEQIEEKFNL
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCC
DYHFKAIDTIFDRVFGQA
CCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVPRYARPAMTAIWEPAARYRIWFEIEAYATEKLGELGVVPPSGAKALWDWWATNPAIDV
CCCCCCCCHHHHHHCCHHHEEEEEEEEHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHH
AAIDAIEAVTKHDVIAFLDWVAQQVGPEARFMHQGMTSSDVLDTTLSVQLARASDILLAD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDALLEAIKRRAFEHKYTPTIGRSHGIHAEPTTFGKKLAEAYAEFTRCRARLVAARAEVA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCAISGAVGTFANIDPSVEEYVAEKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSIERL
HHHHHCCCCCCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPALENVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LWHERDISHSSVERFIGPDATITLDFALARLTSVVDKLVVYPERMQKNLDKMGGLVHSQR
HHHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
VLLALTQAGLERDTAYRLVQRNAMKVWESDGQLNLMELLKADPEVAAALTKEQIEEKFNL
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCC
DYHFKAIDTIFDRVFGQA
CCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]