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The map label for this gene is kefB [H]

Identifier: 87199302

GI number: 87199302

Start: 1322145

End: 1323929

Strand: Reverse

Name: kefB [H]

Synonym: Saro_1281

Alternate gene names: 87199302

Gene position: 1323929-1322145 (Counterclockwise)

Preceding gene: 87199303

Following gene: 87199301

Centisome position: 37.17

GC content: 64.65

Gene sequence:

>1785_bases
ATGGCAGGCAATCTCGAACCGACTACCGCACTTTCCGACGCCCTCGTGATCCTCGGTGCCGCTGGCATCATCATCCCCGC
TTTCGCGCGCTTCCGCATCACCCCGATTATCGGCTTCATCCTCGTCGGTCTCGTTGTCGGCCCGTTCGGCCTGGGATCGC
TCGTGCCGCAGTATCCGTGGCTCTATCATGTCACGATTTCCGATCCGGCCGCGATCACGCCCTTCGCCGAATTCGGCATC
ATCCTGCTACTGTTCGAAATCGGCCTCGAGCTGTCGTTCAACCGCCTGTGGGACATGCGAAGGCTCGTCTTCGGCCTTGG
CGCCATGGAGCTTGGCGTCTCGGCGTTCCTGCTTTCCGCAGTGCTGCTCGGAACGGGTCTCAACATAAGCGGCGCATTCG
GTCTGGGCCTCGCACTAGCCCTTTCATCGACCGCGCTTGTGCTGCCCATTGCCGGAACGCACGGCCCGGTGGGTCGTGCT
GCGCTTTCGATGCTCCTGTTCGAGGATATCGCTCTTGTCCCGATCATCTTCATCCTCGGCGCGATGGGCCCGGTTGCCGC
GCATTCCGACGGTCCCAGCCTCGGCGAGACGCTGCTTTACGGCGGCATCGTCGTAGCTGTCCTGCTGGTCGGCGGCAGGC
TCCTGCTCCCGCGCCTGTTCGCCCAAGCGGCGCAGACGAAGAGCCCCGAGCTGTTCCTTGCCGCCACCATGCTGGTGGTC
ATCGCGGCGGCCCTTTCTACTTCGCTTGTCGGTCTTTCGCCGATCATGGGTGCGCTTCTGGCAGGCCTGCTGATCGCCGA
AACGGAATACCACACCGAGGTCGAATCGATCACCAAGCCATTCAAGGGCCTCGCGCTGGGCGTGTTCCTGATCACCATCG
GCATGGGCATCGACCTGCGCGTTGTCTGGGAGCAGATCGGGCCACTCGCGCTGGCGGTGACCGGGGTTGTGGTGCTCAAG
GCGCTGGTCACGATGTTCGCCCTGCGCGCGATGGGCAAGGAGCGGGCAACCGCGCTGGAAGCCGGCATCCTGATGGCAAG
CCCTTCGGAAACCACGCTGATCGTGCTCGCAACGGCGGCGGAAGCCTCCCTGATCGACCGCGAGACGGCGCAATTCTGGC
AGATCGTCACCGCCATCGGCCTGACCATAACCCCGCTGCTTGCCAAAGTGGGTGAGACCTGGGGCCGCCGCGTGGCCAAG
GGCGGGGCCGACGCCGGACAGGCTGTCGCGGTGGAAGAACCCGAGGGCGAGCGCGTGGTCGTCATGGGCTTCGGTCGCGT
CGGCAGGCTGGTGGCCGACATGCTCTCCGTCCACGGCGCATCCTGGCTGGGCATCGATACCGACCCGGCCATCGTGAAGC
GCGCGCGCAACGACGGATTGCCGGTCCTGTTCGGCAACGTCGACAGCGAAACCGCGATCGAACGGCTTGGTCTGGACAAT
GCGCGCGCAATGATCCTGACGATGGACGAACCGGTACTGGTGGTGCGCATGGTGAAGAAGCTGCGGTCGGGCCATCCCGA
TCTGCCGATCATCGTCCGCGCCCGCGATACCGCCCACGCCGCCGAACTCTACAAGGCAGGAGCCACCCACGCGGTGCCGG
AAACGCTGGAAAGCTCGCTGCAACTGTCGGAAGCGGTTCTGGTCGATCTCGGCTTCCCGATGGGCCTCGTCATCGCCTCC
ATTCACGAGAAGCGCGATGAGTTCCGGGAGCAGATCATGGAGGAAGGCGGCCTTACCGAAAAGCCGCGCCTGAAATCCGC
CTCGCTGCGCGAACGATCCGCGTAA

Upstream 100 bases:

>100_bases
GACAGGGCAAGCATTCTTGCGACGCACTACGCAGTGCCCGGCAACAGTTCGACATGCGGAAAGCCGTGACACCGCCTGCC
CCCTCCCCTAACCTTCGGCG

Downstream 100 bases:

>100_bases
AAGACCGCAACTTTCCGGCCCCCTGCGCGTTCAGCGGGGAGGAGAGTGACCATGACCCACAGCAACGAGCCTGACCTGAA
CCCGGCCACGCAGGGCGATC

Product: sodium/hydrogen exchanger

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MAGNLEPTTALSDALVILGAAGIIIPAFARFRITPIIGFILVGLVVGPFGLGSLVPQYPWLYHVTISDPAAITPFAEFGI
ILLLFEIGLELSFNRLWDMRRLVFGLGAMELGVSAFLLSAVLLGTGLNISGAFGLGLALALSSTALVLPIAGTHGPVGRA
ALSMLLFEDIALVPIIFILGAMGPVAAHSDGPSLGETLLYGGIVVAVLLVGGRLLLPRLFAQAAQTKSPELFLAATMLVV
IAAALSTSLVGLSPIMGALLAGLLIAETEYHTEVESITKPFKGLALGVFLITIGMGIDLRVVWEQIGPLALAVTGVVVLK
ALVTMFALRAMGKERATALEAGILMASPSETTLIVLATAAEASLIDRETAQFWQIVTAIGLTITPLLAKVGETWGRRVAK
GGADAGQAVAVEEPEGERVVVMGFGRVGRLVADMLSVHGASWLGIDTDPAIVKRARNDGLPVLFGNVDSETAIERLGLDN
ARAMILTMDEPVLVVRMVKKLRSGHPDLPIIVRARDTAHAAELYKAGATHAVPETLESSLQLSEAVLVDLGFPMGLVIAS
IHEKRDEFREQIMEEGGLTEKPRLKSASLRERSA

Sequences:

>Translated_594_residues
MAGNLEPTTALSDALVILGAAGIIIPAFARFRITPIIGFILVGLVVGPFGLGSLVPQYPWLYHVTISDPAAITPFAEFGI
ILLLFEIGLELSFNRLWDMRRLVFGLGAMELGVSAFLLSAVLLGTGLNISGAFGLGLALALSSTALVLPIAGTHGPVGRA
ALSMLLFEDIALVPIIFILGAMGPVAAHSDGPSLGETLLYGGIVVAVLLVGGRLLLPRLFAQAAQTKSPELFLAATMLVV
IAAALSTSLVGLSPIMGALLAGLLIAETEYHTEVESITKPFKGLALGVFLITIGMGIDLRVVWEQIGPLALAVTGVVVLK
ALVTMFALRAMGKERATALEAGILMASPSETTLIVLATAAEASLIDRETAQFWQIVTAIGLTITPLLAKVGETWGRRVAK
GGADAGQAVAVEEPEGERVVVMGFGRVGRLVADMLSVHGASWLGIDTDPAIVKRARNDGLPVLFGNVDSETAIERLGLDN
ARAMILTMDEPVLVVRMVKKLRSGHPDLPIIVRARDTAHAAELYKAGATHAVPETLESSLQLSEAVLVDLGFPMGLVIAS
IHEKRDEFREQIMEEGGLTEKPRLKSASLRERSA
>Mature_593_residues
AGNLEPTTALSDALVILGAAGIIIPAFARFRITPIIGFILVGLVVGPFGLGSLVPQYPWLYHVTISDPAAITPFAEFGII
LLLFEIGLELSFNRLWDMRRLVFGLGAMELGVSAFLLSAVLLGTGLNISGAFGLGLALALSSTALVLPIAGTHGPVGRAA
LSMLLFEDIALVPIIFILGAMGPVAAHSDGPSLGETLLYGGIVVAVLLVGGRLLLPRLFAQAAQTKSPELFLAATMLVVI
AAALSTSLVGLSPIMGALLAGLLIAETEYHTEVESITKPFKGLALGVFLITIGMGIDLRVVWEQIGPLALAVTGVVVLKA
LVTMFALRAMGKERATALEAGILMASPSETTLIVLATAAEASLIDRETAQFWQIVTAIGLTITPLLAKVGETWGRRVAKG
GADAGQAVAVEEPEGERVVVMGFGRVGRLVADMLSVHGASWLGIDTDPAIVKRARNDGLPVLFGNVDSETAIERLGLDNA
RAMILTMDEPVLVVRMVKKLRSGHPDLPIIVRARDTAHAAELYKAGATHAVPETLESSLQLSEAVLVDLGFPMGLVIASI
HEKRDEFREQIMEEGGLTEKPRLKSASLRERSA

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1786232, Length=600, Percent_Identity=30.3333333333333, Blast_Score=242, Evalue=6e-65,
Organism=Escherichia coli, GI1789749, Length=571, Percent_Identity=30.8231173380035, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1786685, Length=528, Percent_Identity=24.4318181818182, Blast_Score=122, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 62468; Mature: 62336

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNLEPTTALSDALVILGAAGIIIPAFARFRITPIIGFILVGLVVGPFGLGSLVPQYPW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
LYHVTISDPAAITPFAEFGIILLLFEIGLELSFNRLWDMRRLVFGLGAMELGVSAFLLSA
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VLLGTGLNISGAFGLGLALALSSTALVLPIAGTHGPVGRAALSMLLFEDIALVPIIFILG
HHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AMGPVAAHSDGPSLGETLLYGGIVVAVLLVGGRLLLPRLFAQAAQTKSPELFLAATMLVV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IAAALSTSLVGLSPIMGALLAGLLIAETEYHTEVESITKPFKGLALGVFLITIGMGIDLR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VVWEQIGPLALAVTGVVVLKALVTMFALRAMGKERATALEAGILMASPSETTLIVLATAA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCEEEECCCCCEEEEEEECC
EASLIDRETAQFWQIVTAIGLTITPLLAKVGETWGRRVAKGGADAGQAVAVEEPEGERVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEE
VMGFGRVGRLVADMLSVHGASWLGIDTDPAIVKRARNDGLPVLFGNVDSETAIERLGLDN
EEECCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCC
ARAMILTMDEPVLVVRMVKKLRSGHPDLPIIVRARDTAHAAELYKAGATHAVPETLESSL
CCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHH
QLSEAVLVDLGFPMGLVIASIHEKRDEFREQIMEEGGLTEKPRLKSASLRERSA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
AGNLEPTTALSDALVILGAAGIIIPAFARFRITPIIGFILVGLVVGPFGLGSLVPQYPW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
LYHVTISDPAAITPFAEFGIILLLFEIGLELSFNRLWDMRRLVFGLGAMELGVSAFLLSA
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VLLGTGLNISGAFGLGLALALSSTALVLPIAGTHGPVGRAALSMLLFEDIALVPIIFILG
HHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AMGPVAAHSDGPSLGETLLYGGIVVAVLLVGGRLLLPRLFAQAAQTKSPELFLAATMLVV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IAAALSTSLVGLSPIMGALLAGLLIAETEYHTEVESITKPFKGLALGVFLITIGMGIDLR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VVWEQIGPLALAVTGVVVLKALVTMFALRAMGKERATALEAGILMASPSETTLIVLATAA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCEEEECCCCCEEEEEEECC
EASLIDRETAQFWQIVTAIGLTITPLLAKVGETWGRRVAKGGADAGQAVAVEEPEGERVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEE
VMGFGRVGRLVADMLSVHGASWLGIDTDPAIVKRARNDGLPVLFGNVDSETAIERLGLDN
EEECCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCC
ARAMILTMDEPVLVVRMVKKLRSGHPDLPIIVRARDTAHAAELYKAGATHAVPETLESSL
CCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHH
QLSEAVLVDLGFPMGLVIASIHEKRDEFREQIMEEGGLTEKPRLKSASLRERSA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA