BacMap

Definition	Novosphingobium aromaticivorans DSM 12444 chromosome, complete genome.
Accession	NC_007794
Length	3,561,584

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The map label for this gene is tuf

Identifier: 87199268

GI number: 87199268

Start: 1296706

End: 1297896

Strand: Direct

Name: tuf

Synonym: Saro_1247

Alternate gene names: 87199268

Gene position: 1296706-1297896 (Clockwise)

Preceding gene: 87199267

Following gene: 87199269

Centisome position: 36.41

GC content: 63.39

Gene sequence:

>1191_bases
ATGGCCAAGGCTAAGTTTGAGCGGAACAAGCCGCACTGCAACATCGGCACCATCGGTCACGTCGACCACGGCAAGACCAC
GCTGACCGCCGCGATCACCAAGGTGCTCGCCGAGCAGGGTGGCGCTGAATTCACCGACTACGCGAACATCGACAAGGCTC
CGGAAGAGCGCGAGCGCGGCATCACCATCTCGACCGCACACGTCGAGTACGAGACCGCCAACCGCCACTACGCGCACGTC
GACTGCCCGGGTCACGCCGACTACGTGAAGAACATGATCACCGGTGCCGCCCAGATGGACGGCGCGATCCTGGTCGTGAA
CGCTGCTGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTTGCCCGCCAGGTCGGCGTGCCCGCGCTCGTCGTGT
ACATGAACAAGGTCGACCAGGTCGACGACGAGGAAATCCTCGAGCTCGTCGAACTCGAAGTTCGCGAACTGCTGTCGTCG
TACGACTTCCCGGGCGACGATATTCCGATCGTCAAGGGTTCGGCTCTGGCCGCTCTCGAAGGCCGCGACGACAACATCGG
CAAGGACTCGATCAACGCGCTCATGGCTGCCGTTGACGCGTACATCCCGCAGCCGCCGCGTCCGACCGACAAGCCCTTCC
TGATGCCCGTCGAAGACGTGTTCTCGATCTCGGGTCGCGGCACCGTGGTGACCGGCCGTATCGAAACCGGCATCATCAAG
GTCGGCGAAGAAGTCGAGATCATCGGTCTGAAGGACACCCAGAAGACCACCGTGACCGGCGTCGAAATGTTCCGCAAGCT
GCTCGACCAGGGCGAAGCCGGCGACAACATCGGTGCGCTGATCCGCGGCATCAAGCGTGAAGAAGTCGAGCGTGGGCAGG
TTCTCGCCAAGCCCGGTTCGGTGACCCCGCACACCGAATTCTCGGCCGAGGTCTACGTGCTGTCGAAGGACGAAGGTGGC
CGTCACACGCCGTTCTTCGCCAACTACCGCCCGCAGTTCTACTTCCGCACCACCGACGTCACCGGTGAGGTCGTCCTCCC
CGAGGGCACCGAGATGGTGATGCCGGGCGACAACGTCACCCTGGCTGTCAAGCTGATCGCTCCGATCGCGATGGACGAAG
GTCTGCGCTTCGCAATTCGCGAAGGCGGCCGCACCGTCGGTTCGGGGGTTGTCAGCAAGATCACGAAGTAA

Upstream 100 bases:

>100_bases
TTGCATAAGAGCACGGATAGCATTAGGGGCGGCGCTCGATTCAGCGGGTGCCGTCCAATTCCCGCAGTATCTGCACACTT
GAGAACAGAAGGTTTGAACA

Downstream 100 bases:

>100_bases
TATAGGCCGCGCACCGGCGGGGAGATTCGCTCTTCCCGCCGGTCGGTTTTTTACTGACTGATCGCTATTTGGCAGTCGGC
CAGCTCTCCCCGAGCTTCAG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERNKPHCNIGTIGHVDHGKTTLTAAITKVLAEQGGAEFTDYANIDKAPEERERGITISTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLARQVGVPALVVYMNKVDQVDDEEILELVELEVRELLSS
YDFPGDDIPIVKGSALAALEGRDDNIGKDSINALMAAVDAYIPQPPRPTDKPFLMPVEDVFSISGRGTVVTGRIETGIIK
VGEEVEIIGLKDTQKTTVTGVEMFRKLLDQGEAGDNIGALIRGIKREEVERGQVLAKPGSVTPHTEFSAEVYVLSKDEGG
RHTPFFANYRPQFYFRTTDVTGEVVLPEGTEMVMPGDNVTLAVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITK

Sequences:

>Translated_396_residues
MAKAKFERNKPHCNIGTIGHVDHGKTTLTAAITKVLAEQGGAEFTDYANIDKAPEERERGITISTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLARQVGVPALVVYMNKVDQVDDEEILELVELEVRELLSS
YDFPGDDIPIVKGSALAALEGRDDNIGKDSINALMAAVDAYIPQPPRPTDKPFLMPVEDVFSISGRGTVVTGRIETGIIK
VGEEVEIIGLKDTQKTTVTGVEMFRKLLDQGEAGDNIGALIRGIKREEVERGQVLAKPGSVTPHTEFSAEVYVLSKDEGG
RHTPFFANYRPQFYFRTTDVTGEVVLPEGTEMVMPGDNVTLAVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITK
>Mature_395_residues
AKAKFERNKPHCNIGTIGHVDHGKTTLTAAITKVLAEQGGAEFTDYANIDKAPEERERGITISTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLARQVGVPALVVYMNKVDQVDDEEILELVELEVRELLSSY
DFPGDDIPIVKGSALAALEGRDDNIGKDSINALMAAVDAYIPQPPRPTDKPFLMPVEDVFSISGRGTVVTGRIETGIIKV
GEEVEIIGLKDTQKTTVTGVEMFRKLLDQGEAGDNIGALIRGIKREEVERGQVLAKPGSVTPHTEFSAEVYVLSKDEGGR
HTPFFANYRPQFYFRTTDVTGEVVLPEGTEMVMPGDNVTLAVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=58.8235294117647, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI4503471, Length=455, Percent_Identity=30.989010989011, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI4503475, Length=455, Percent_Identity=30.1098901098901, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=30.0683371298405, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=30.5239179954442, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI54607086, Length=328, Percent_Identity=29.5731707317073, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=30.0595238095238, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=30.0595238095238, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=30.0595238095238, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI46094014, Length=323, Percent_Identity=30.3405572755418, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI4503507, Length=288, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI157426893, Length=278, Percent_Identity=26.2589928057554, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=33.0827067669173, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=74.8730964467005, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=74.8730964467005, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=31.7507418397626, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994988, Length=272, Percent_Identity=33.0882352941176, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789108, Length=265, Percent_Identity=31.3207547169811, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=31.7241379310345, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=52.0100502512563, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.1524547803618, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=31.3333333333333, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=31.3333333333333, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=29.4663573085847, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=27.3348519362187, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=27.3348519362187, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=349, Percent_Identity=30.0859598853868, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=30.1470588235294, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=41.4965986394558, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=280, Percent_Identity=26.0714285714286, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=26.890756302521, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556745, Length=135, Percent_Identity=31.1111111111111, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.9191919191919, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=31.7673378076063, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=31.7673378076063, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=25.3897550111359, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=29.2753623188406, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=281, Percent_Identity=28.1138790035587, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=248, Percent_Identity=28.2258064516129, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=188, Percent_Identity=31.3829787234043, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=58.7179487179487, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=58.7179487179487, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=48.7046632124352, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=30.4444444444444, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=30.4444444444444, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=30.4444444444444, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=30.4444444444444, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=31.4031180400891, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=31.4031180400891, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.6831683168317, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=27.8538812785388, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=30.1775147928994, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=27.8571428571429, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=27.8571428571429, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=34.4594594594595, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=133, Percent_Identity=35.3383458646617, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_NOVAD (Q2G8Y2)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496525.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q2G8Y2
- SMR:   Q2G8Y2
- STRING:   Q2G8Y2
- GeneID:   3917878
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1247
- NMPDR:   fig|48935.1.peg.1785
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q2G8Y2
- ProtClustDB:   PRK00049
- BioCyc:   NARO279238:SARO_1247-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 42977; Mature: 42846

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHCNIGTIGHVDHGKTTLTAAITKVLAEQGGAEFTDYANIDKAPEERERG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
ITISTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVYMNKVDQVDDEEILELVELEVRELLSSYDFPGDDIPIVKGSALAALE
HHHHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
GRDDNIGKDSINALMAAVDAYIPQPPRPTDKPFLMPVEDVFSISGRGTVVTGRIETGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEEEECEEE
VGEEVEIIGLKDTQKTTVTGVEMFRKLLDQGEAGDNIGALIRGIKREEVERGQVLAKPGS
ECCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
VTPHTEFSAEVYVLSKDEGGRHTPFFANYRPQFYFRTTDVTGEVVLPEGTEMVMPGDNVT
CCCCCCCCEEEEEEECCCCCCCCCEEECCCCEEEEEEECCCCEEECCCCCEEEECCCCEE
LAVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITK
EEEEEEEHHHHCCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERNKPHCNIGTIGHVDHGKTTLTAAITKVLAEQGGAEFTDYANIDKAPEERERG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
ITISTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVYMNKVDQVDDEEILELVELEVRELLSSYDFPGDDIPIVKGSALAALE
HHHHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
GRDDNIGKDSINALMAAVDAYIPQPPRPTDKPFLMPVEDVFSISGRGTVVTGRIETGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEEEECEEE
VGEEVEIIGLKDTQKTTVTGVEMFRKLLDQGEAGDNIGALIRGIKREEVERGQVLAKPGS
ECCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
VTPHTEFSAEVYVLSKDEGGRHTPFFANYRPQFYFRTTDVTGEVVLPEGTEMVMPGDNVT
CCCCCCCCEEEEEEECCCCCCCCCEEECCCCEEEEEEECCCCEEECCCCCEEEECCCCEE
LAVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITK
EEEEEEEHHHHCCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA