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The map label for this gene is mexA [H]

Identifier: 87199240

GI number: 87199240

Start: 1267442

End: 1268650

Strand: Direct

Name: mexA [H]

Synonym: Saro_1218

Alternate gene names: 87199240

Gene position: 1267442-1268650 (Clockwise)

Preceding gene: 87199233

Following gene: 87199241

Centisome position: 35.59

GC content: 67.74

Gene sequence:

>1209_bases
ATGGTGCGTTCCGATCCAGCGAGGCTGTCCTTGCCGTCCACAATCCGATATCCGCTCTTCGCGTTTGCCGCTGCCGCTTC
CTTTGCGCTGGCTGGTTGCGGTTCCTCCGCCGAACCTGCCGGCAAACCCGCGCCCACTGTCGGCGTCGTGATCGCCCGCC
AGCAGCCGGTCGAGCTTACCGCCGAACTTTCAGGACGAACCGAAGCGTCCGAAGTCGCGGAAGTGCGTCCGCAAGTTGCC
GGGATCGTCATGGCCCGCCTGTTCCCCGAAGGTTCGTTCGTTCGCGCCGGGCAGCCGCTCTACCAGATCGATTCCCGTAT
CTATGCCGCGTCCAGCGCGCAGGCGGCGGCATCGCTGGCATCCGCGCAGGCCACGGTCGAGGCCAACCGCCTGAAGGCGG
AGCGCTTTCGCCTCCTTGCCGAACAGGGCGGCGTGAGCCGGCAGGATGCGGCCGATGCCCTGGCGGCATACAACCAGTCG
CGCGCCGCGGTGGAGCAGGCCCGCGCTTCGCTCGCTTCCGCGCGGGTCAATCTGGGCTTCACCCGGGTCACGTCCCCCAT
TTCGGGGCGCATCGGCATTTCGATGGTGACCAAGGGCGCGCTCGTCACTGCCAACCAGGCCGAACCGATGGCCAAGGTCC
AACGGCTCGACCCGATGTACGTCAACATCAAGCAGTCCGGCGCTGATTTCATCGCCCTGCGCCGCGCTCTGGAAAGCGGC
AAGCTGGTGTCCGGGGCGGCCCCCGTTCGCGTGATATTGCCCGACGGTTCGGAATATCCGGTTACCGGCAGGCTCAATCC
CGCCGACATCGACGTCGATCCCGAGACCGGCACGATCACCGTCCGGGCCGTGGTGCCAAACCCGAGAGGGGATCTTCTGC
CGGGGCTGTTCGTCCGCGCGCGCGTCGGGCAGGGCACCGTGCCAAACGGTATCCTCGTGCCTCAGGCTGCCGTCAGCCGC
GATCCGCGTGGCCGTGCCAGCGTGCTCGTCGCCGGTGCGGGAGACATGCTCGAGAAGCGCGAGATCGTGGCGGAAACGCC
GGTCGGCCAGAACTGGCTCGTCACCGGCGGGCTCAAGCCGGGCGACCGCGTCGTTGTCGAAGGACTGATCAACGCGCGCG
AAGGGCAGAAGGCCAAGGTCGTCGCCGCCGGCAGCAAGGCGGCGTCTGATGCAACGGCCCCGTCCGATGCAAAGGGCGCG
GGGAAGTAA

Upstream 100 bases:

>100_bases
CAATCTGCTAGACACGCTCAAGAAACCGGTCCCGGGCCGGTCGCCGCGCATTGATCCGCCCACCTTGCGATTCGACGCGC
GCCGACCGTTCCGCCGCCGG

Downstream 100 bases:

>100_bases
CCGCCGATGATTTCGCGCTTCTTCGCCCACCGCCCGATTTTCGCCTGGGTTCTTGCCATCGTCATCATGGCGGCGGGCCT
GTTCGCCGTCAGCACCATGG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MVRSDPARLSLPSTIRYPLFAFAAAASFALAGCGSSAEPAGKPAPTVGVVIARQQPVELTAELSGRTEASEVAEVRPQVA
GIVMARLFPEGSFVRAGQPLYQIDSRIYAASSAQAAASLASAQATVEANRLKAERFRLLAEQGGVSRQDAADALAAYNQS
RAAVEQARASLASARVNLGFTRVTSPISGRIGISMVTKGALVTANQAEPMAKVQRLDPMYVNIKQSGADFIALRRALESG
KLVSGAAPVRVILPDGSEYPVTGRLNPADIDVDPETGTITVRAVVPNPRGDLLPGLFVRARVGQGTVPNGILVPQAAVSR
DPRGRASVLVAGAGDMLEKREIVAETPVGQNWLVTGGLKPGDRVVVEGLINAREGQKAKVVAAGSKAASDATAPSDAKGA
GK

Sequences:

>Translated_402_residues
MVRSDPARLSLPSTIRYPLFAFAAAASFALAGCGSSAEPAGKPAPTVGVVIARQQPVELTAELSGRTEASEVAEVRPQVA
GIVMARLFPEGSFVRAGQPLYQIDSRIYAASSAQAAASLASAQATVEANRLKAERFRLLAEQGGVSRQDAADALAAYNQS
RAAVEQARASLASARVNLGFTRVTSPISGRIGISMVTKGALVTANQAEPMAKVQRLDPMYVNIKQSGADFIALRRALESG
KLVSGAAPVRVILPDGSEYPVTGRLNPADIDVDPETGTITVRAVVPNPRGDLLPGLFVRARVGQGTVPNGILVPQAAVSR
DPRGRASVLVAGAGDMLEKREIVAETPVGQNWLVTGGLKPGDRVVVEGLINAREGQKAKVVAAGSKAASDATAPSDAKGA
GK
>Mature_402_residues
MVRSDPARLSLPSTIRYPLFAFAAAASFALAGCGSSAEPAGKPAPTVGVVIARQQPVELTAELSGRTEASEVAEVRPQVA
GIVMARLFPEGSFVRAGQPLYQIDSRIYAASSAQAAASLASAQATVEANRLKAERFRLLAEQGGVSRQDAADALAAYNQS
RAAVEQARASLASARVNLGFTRVTSPISGRIGISMVTKGALVTANQAEPMAKVQRLDPMYVNIKQSGADFIALRRALESG
KLVSGAAPVRVILPDGSEYPVTGRLNPADIDVDPETGTITVRAVVPNPRGDLLPGLFVRARVGQGTVPNGILVPQAAVSR
DPRGRASVLVAGAGDMLEKREIVAETPVGQNWLVTGGLKPGDRVVVEGLINAREGQKAKVVAAGSKAASDATAPSDAKGA
GK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=380, Percent_Identity=47.6315789473684, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1789665, Length=365, Percent_Identity=45.7534246575342, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789929, Length=363, Percent_Identity=43.801652892562, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI87082036, Length=331, Percent_Identity=29.607250755287, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081796, Length=362, Percent_Identity=24.585635359116, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41541; Mature: 41541

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRSDPARLSLPSTIRYPLFAFAAAASFALAGCGSSAEPAGKPAPTVGVVIARQQPVELT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEE
AELSGRTEASEVAEVRPQVAGIVMARLFPEGSFVRAGQPLYQIDSRIYAASSAQAAASLA
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCHHEECCCHHHHHHHH
SAQATVEANRLKAERFRLLAEQGGVSRQDAADALAAYNQSRAAVEQARASLASARVNLGF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TRVTSPISGRIGISMVTKGALVTANQAEPMAKVQRLDPMYVNIKQSGADFIALRRALESG
HEECCCCCCCCEEEEEECCEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCC
KLVSGAAPVRVILPDGSEYPVTGRLNPADIDVDPETGTITVRAVVPNPRGDLLPGLFVRA
CEEECCCCEEEEECCCCCCCEECCCCCCCCEECCCCCEEEEEEEECCCCCCCCCCEEEEE
RVGQGTVPNGILVPQAAVSRDPRGRASVLVAGAGDMLEKREIVAETPVGQNWLVTGGLKP
ECCCCCCCCCEECCCHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEEECCCCC
GDRVVVEGLINAREGQKAKVVAAGSKAASDATAPSDAKGAGK
CCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVRSDPARLSLPSTIRYPLFAFAAAASFALAGCGSSAEPAGKPAPTVGVVIARQQPVELT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEE
AELSGRTEASEVAEVRPQVAGIVMARLFPEGSFVRAGQPLYQIDSRIYAASSAQAAASLA
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCHHEECCCHHHHHHHH
SAQATVEANRLKAERFRLLAEQGGVSRQDAADALAAYNQSRAAVEQARASLASARVNLGF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TRVTSPISGRIGISMVTKGALVTANQAEPMAKVQRLDPMYVNIKQSGADFIALRRALESG
HEECCCCCCCCEEEEEECCEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCC
KLVSGAAPVRVILPDGSEYPVTGRLNPADIDVDPETGTITVRAVVPNPRGDLLPGLFVRA
CEEECCCCEEEEECCCCCCCEECCCCCCCCEECCCCCEEEEEEEECCCCCCCCCCEEEEE
RVGQGTVPNGILVPQAAVSRDPRGRASVLVAGAGDMLEKREIVAETPVGQNWLVTGGLKP
ECCCCCCCCCEECCCHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEEECCCCC
GDRVVVEGLINAREGQKAKVVAAGSKAASDATAPSDAKGAGK
CCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]