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The map label for this gene is actII-2 [H]

Identifier: 87199231

GI number: 87199231

Start: 1256692

End: 1258152

Strand: Direct

Name: actII-2 [H]

Synonym: Saro_1209

Alternate gene names: 87199231

Gene position: 1256692-1258152 (Clockwise)

Preceding gene: 87199230

Following gene: 87199232

Centisome position: 35.28

GC content: 69.06

Gene sequence:

>1461_bases
ATGGCAAATGACATCGCCGGCATGACCGAGAGGCAGAAGAGCCTCGCCTTCTTCACGCTCATCACCGCGCTGGTGCTGGA
GATCGTGGACGTCACGATCATCAACACCGCGCTGCCGGCCATGCAGGCCGACTTTGCGGCGAGAGGGCAGGACCTTGGGG
GCGATACCGCGCAGTGGATCGCGGCGGGCTATTCGCTCGCCTTCGGGTTGCTGCTGATCCTTGGCGGCAGGTTGGGGGAC
CTGTTCGGCGGGCGGGCGATGTTCCTGGCGGGCGTGGGCGGATTCACCGTGGCATCGGCGCTTTGCGGCATGGCATCGGA
CCCCGGACTGCTGGTCGCTGCGCGTGTTCTCCAGGGGGCGGCAGGCGCGATCATGGCGCCGCAGGTCATGGCGGTGATCC
AGATCCTCTACGATCCGGTGGAGCGCATCGGACGCCTGGCCTGGTTCGGGGTGATCGGCGGTCTGGCCGGCATCGCCGGG
CCGATCCTTGGCGGCCTGCTGATTGCGGCCGACATTGCCGGGCTCGGCTGGCGCACGGTGTTCCTCATCAACCTGCCCAT
CGGCCTTGCGGCGCTTGCCGCGGGCTGGCGGTTCCTGCCGCGCGAGGTGGCGCACAAGGGCGGGCGGATCGACGTGATCG
GCACACTTTCCTTCGGCGCGGCGCTTGCGGCGATGCTGTTTCCGCTGGTGCGCGGGGAACATGTCGGCATGGATACCGCG
AGCATGGCGCTGCTGGCGGCATCGCCCCTGCTGATGTGGGCGGCGTGGCGCGGTCTGGTGCGCCGGGCCGGGGCGGGGCG
CCCGGTGATCTTCGATCCCGAATTGCTGCACGACGGCCTGTTCCGCACAGGCGCGCTGATCGGCCTCGTTTTCTCGGCGG
CCAACACCGGCTTCCTGTTCGTGTTCGCGCACGCTCTCCAGTCGCGGCTCGGCTACAGCCCCCTGCAGACTGGACTGGTG
CATATCCCCTTCAGCGCCGGGGTCATGGTCGGCATGGGATTTCTCGGGCGGCGCTACCTGGCGCGGGCAGGCAAGTGGGT
CATGGTCTGCGCGGCGCTGGCGCTGCTTGTGGCCGACAGCGCCGCGCTTGGCTGGATCGCGCTTGGCGGGCACGACCTGT
TCCTCCTGTTGCCGGCGCTGCTGCTGGCTGGCGTAGGCATGGGTACGCTTTCCGGGCCGGTTTCGCCCGTCGCGCTTGCC
AGAGTGGACCGGCGCCATGCGGGGGCTGCCAGCGGCATCCTCAAGACCGTGCTGCAGATGGGCAGTGCGTTCGGCATCGC
GCTGGTGGGAAGCGCCTACTTCGCGTTGCGCGGGCCGGTCGCCGGGGCGGGGGCGGGAGGCGTATATGCGGCGCTGGCGG
TGATCCTCGTGCTGCTGGTGGTGTGCCTGGTGCTTGCGCTGCTGCTGCCGGCGCGGATCTTTGCGCCGGAACCATCTTCC
AAAGCCGCGCAAACCGGATAA

Upstream 100 bases:

>100_bases
TCCTCGGCCTGCCAGAACCCTTCGCTCACCTACATGGCGCTGACCGCACGTGCCTGCGATGCGGCGGTCAGGATGCTGCG
CGAAGGTGCGATCTGAGCCG

Downstream 100 bases:

>100_bases
GCTGGCGCGCGAAACACGCACTGCGGGCTGGAGACTCGCAGGCGTGGGAAGAGGAAGCGCGCAATGGACGATCTGCCGAC
GGCCAAGTTCCACACCATGA

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MANDIAGMTERQKSLAFFTLITALVLEIVDVTIINTALPAMQADFAARGQDLGGDTAQWIAAGYSLAFGLLLILGGRLGD
LFGGRAMFLAGVGGFTVASALCGMASDPGLLVAARVLQGAAGAIMAPQVMAVIQILYDPVERIGRLAWFGVIGGLAGIAG
PILGGLLIAADIAGLGWRTVFLINLPIGLAALAAGWRFLPREVAHKGGRIDVIGTLSFGAALAAMLFPLVRGEHVGMDTA
SMALLAASPLLMWAAWRGLVRRAGAGRPVIFDPELLHDGLFRTGALIGLVFSAANTGFLFVFAHALQSRLGYSPLQTGLV
HIPFSAGVMVGMGFLGRRYLARAGKWVMVCAALALLVADSAALGWIALGGHDLFLLLPALLLAGVGMGTLSGPVSPVALA
RVDRRHAGAASGILKTVLQMGSAFGIALVGSAYFALRGPVAGAGAGGVYAALAVILVLLVVCLVLALLLPARIFAPEPSS
KAAQTG

Sequences:

>Translated_486_residues
MANDIAGMTERQKSLAFFTLITALVLEIVDVTIINTALPAMQADFAARGQDLGGDTAQWIAAGYSLAFGLLLILGGRLGD
LFGGRAMFLAGVGGFTVASALCGMASDPGLLVAARVLQGAAGAIMAPQVMAVIQILYDPVERIGRLAWFGVIGGLAGIAG
PILGGLLIAADIAGLGWRTVFLINLPIGLAALAAGWRFLPREVAHKGGRIDVIGTLSFGAALAAMLFPLVRGEHVGMDTA
SMALLAASPLLMWAAWRGLVRRAGAGRPVIFDPELLHDGLFRTGALIGLVFSAANTGFLFVFAHALQSRLGYSPLQTGLV
HIPFSAGVMVGMGFLGRRYLARAGKWVMVCAALALLVADSAALGWIALGGHDLFLLLPALLLAGVGMGTLSGPVSPVALA
RVDRRHAGAASGILKTVLQMGSAFGIALVGSAYFALRGPVAGAGAGGVYAALAVILVLLVVCLVLALLLPARIFAPEPSS
KAAQTG
>Mature_485_residues
ANDIAGMTERQKSLAFFTLITALVLEIVDVTIINTALPAMQADFAARGQDLGGDTAQWIAAGYSLAFGLLLILGGRLGDL
FGGRAMFLAGVGGFTVASALCGMASDPGLLVAARVLQGAAGAIMAPQVMAVIQILYDPVERIGRLAWFGVIGGLAGIAGP
ILGGLLIAADIAGLGWRTVFLINLPIGLAALAAGWRFLPREVAHKGGRIDVIGTLSFGAALAAMLFPLVRGEHVGMDTAS
MALLAASPLLMWAAWRGLVRRAGAGRPVIFDPELLHDGLFRTGALIGLVFSAANTGFLFVFAHALQSRLGYSPLQTGLVH
IPFSAGVMVGMGFLGRRYLARAGKWVMVCAALALLVADSAALGWIALGGHDLFLLLPALLLAGVGMGTLSGPVSPVALAR
VDRRHAGAASGILKTVLQMGSAFGIALVGSAYFALRGPVAGAGAGGVYAALAVILVLLVVCLVLALLLPARIFAPEPSSK
AAQTG

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=430, Percent_Identity=27.6744186046512, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790195, Length=429, Percent_Identity=25.4079254079254, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788392, Length=470, Percent_Identity=26.1702127659574, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789042, Length=172, Percent_Identity=30.8139534883721, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788710, Length=303, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49698; Mature: 49567

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDIAGMTERQKSLAFFTLITALVLEIVDVTIINTALPAMQADFAARGQDLGGDTAQWI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AAGYSLAFGLLLILGGRLGDLFGGRAMFLAGVGGFTVASALCGMASDPGLLVAARVLQGA
HHHHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
AGAIMAPQVMAVIQILYDPVERIGRLAWFGVIGGLAGIAGPILGGLLIAADIAGLGWRTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FLINLPIGLAALAAGWRFLPREVAHKGGRIDVIGTLSFGAALAAMLFPLVRGEHVGMDTA
EEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCHH
SMALLAASPLLMWAAWRGLVRRAGAGRPVIFDPELLHDGLFRTGALIGLVFSAANTGFLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFAHALQSRLGYSPLQTGLVHIPFSAGVMVGMGFLGRRYLARAGKWVMVCAALALLVADS
HHHHHHHHHCCCCCHHHCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
AALGWIALGGHDLFLLLPALLLAGVGMGTLSGPVSPVALARVDRRHAGAASGILKTVLQM
CHHCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
GSAFGIALVGSAYFALRGPVAGAGAGGVYAALAVILVLLVVCLVLALLLPARIFAPEPSS
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KAAQTG
CCCCCC
>Mature Secondary Structure 
ANDIAGMTERQKSLAFFTLITALVLEIVDVTIINTALPAMQADFAARGQDLGGDTAQWI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AAGYSLAFGLLLILGGRLGDLFGGRAMFLAGVGGFTVASALCGMASDPGLLVAARVLQGA
HHHHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
AGAIMAPQVMAVIQILYDPVERIGRLAWFGVIGGLAGIAGPILGGLLIAADIAGLGWRTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FLINLPIGLAALAAGWRFLPREVAHKGGRIDVIGTLSFGAALAAMLFPLVRGEHVGMDTA
EEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCHH
SMALLAASPLLMWAAWRGLVRRAGAGRPVIFDPELLHDGLFRTGALIGLVFSAANTGFLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFAHALQSRLGYSPLQTGLVHIPFSAGVMVGMGFLGRRYLARAGKWVMVCAALALLVADS
HHHHHHHHHCCCCCHHHCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
AALGWIALGGHDLFLLLPALLLAGVGMGTLSGPVSPVALARVDRRHAGAASGILKTVLQM
CHHCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
GSAFGIALVGSAYFALRGPVAGAGAGGVYAALAVILVLLVVCLVLALLLPARIFAPEPSS
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KAAQTG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]