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The map label for this gene is leuD

Identifier: 87199195

GI number: 87199195

Start: 1213375

End: 1213983

Strand: Direct

Name: leuD

Synonym: Saro_1173

Alternate gene names: 87199195

Gene position: 1213375-1213983 (Clockwise)

Preceding gene: 87199194

Following gene: 87199196

Centisome position: 34.07

GC content: 64.86

Gene sequence:

>609_bases
ATGGAACCGGTGAAGCAGATCGAGGGACGGGCTATCCCGTTCGGCCGCAAGAACGTCGATACCGACGTGATCATTCCCGC
CAGGTGGCTCAAGACGATTACCCGTCAGGGCCTTGGTCGCGGTGCGTTCGAGGCGCTGCGCGCCGATCCTGACAACATCT
TCGACAGCGCCGAGTTCGCCGGCTCGCCGATCCTCATCGCGGGCGACAACTTCGGCTGCGGCTCCAGCCGCGAACACGCC
GCCTGGGCGCTGCTCGACATGGGCGTGAAGGCGGTCATCGCGCCGTCGTTTTCCGACATCTTCTCGGGCAACGCGTTCAA
GAACGGCATCCTCACCGTCGTCCTCCCGCAGGAGGCCATAGACCGGCTCATGGAAGTCGCGCAGACCGATCCCGTCTCGA
TCGACCTCGAGGCGCAGACCGTCACCACCCCGTTCCAGGATCGCTTCTCGTTCGAGATCGACCCCTTCCGCAAGCACTGC
CTTGCCAACGGCCTCGACGAGGTCGGCCTGACCATGGCGCGCGGCGATGCCATTGCCACGCACGAGGCCCGAATGCGGGA
AAGCCTCCCGTTCCTTGCCAAGGGAACCGATGCCGTAGCGGCAGCTTAA

Upstream 100 bases:

>100_bases
CCAAGACCCCTCCGTCCGACACCCCCGAGACGGACTGACCCGCAACATTCTTGCGCCAAACCCCGGTTGCATCACCGATT
GTGCAAGCTATAGCGACGGC

Downstream 100 bases:

>100_bases
GTGCTCGGTTAAGGGGGCTTCCCAAGCCGTCCAAAACTTGCTTCCCTGTGGCCAAGCGGACCGATTCCGCATGGCTTAGG
GAGAATGAACGATGAAGGCT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MEPVKQIEGRAIPFGRKNVDTDVIIPARWLKTITRQGLGRGAFEALRADPDNIFDSAEFAGSPILIAGDNFGCGSSREHA
AWALLDMGVKAVIAPSFSDIFSGNAFKNGILTVVLPQEAIDRLMEVAQTDPVSIDLEAQTVTTPFQDRFSFEIDPFRKHC
LANGLDEVGLTMARGDAIATHEARMRESLPFLAKGTDAVAAA

Sequences:

>Translated_202_residues
MEPVKQIEGRAIPFGRKNVDTDVIIPARWLKTITRQGLGRGAFEALRADPDNIFDSAEFAGSPILIAGDNFGCGSSREHA
AWALLDMGVKAVIAPSFSDIFSGNAFKNGILTVVLPQEAIDRLMEVAQTDPVSIDLEAQTVTTPFQDRFSFEIDPFRKHC
LANGLDEVGLTMARGDAIATHEARMRESLPFLAKGTDAVAAA
>Mature_202_residues
MEPVKQIEGRAIPFGRKNVDTDVIIPARWLKTITRQGLGRGAFEALRADPDNIFDSAEFAGSPILIAGDNFGCGSSREHA
AWALLDMGVKAVIAPSFSDIFSGNAFKNGILTVVLPQEAIDRLMEVAQTDPVSIDLEAQTVTTPFQDRFSFEIDPFRKHC
LANGLDEVGLTMARGDAIATHEARMRESLPFLAKGTDAVAAA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=187, Percent_Identity=45.4545454545455, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321429, Length=210, Percent_Identity=43.3333333333333, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320440, Length=129, Percent_Identity=32.5581395348837, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_NOVAD (Q2G955)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496452.1
- ProteinModelPortal:   Q2G955
- SMR:   Q2G955
- STRING:   Q2G955
- GeneID:   3916470
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1173
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q2G955
- ProtClustDB:   PRK01641
- BioCyc:   NARO279238:SARO_1173-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21817; Mature: 21817

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPVKQIEGRAIPFGRKNVDTDVIIPARWLKTITRQGLGRGAFEALRADPDNIFDSAEFA
CCCHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCHHHC
GSPILIAGDNFGCGSSREHAAWALLDMGVKAVIAPSFSDIFSGNAFKNGILTVVLPQEAI
CCCEEEECCCCCCCCCCCHHHHHHHHHCCCEEECCCHHHHHCCCCCCCCCEEEEECHHHH
DRLMEVAQTDPVSIDLEAQTVTTPFQDRFSFEIDPFRKHCLANGLDEVGLTMARGDAIAT
HHHHHHHCCCCCEEEEECCEECCCCCCCCCCCCCHHHHHHHHCCHHHHCEEEECCCCHHH
HEARMRESLPFLAKGTDAVAAA
HHHHHHHCCCCCCCCCCHHHCC
>Mature Secondary Structure
MEPVKQIEGRAIPFGRKNVDTDVIIPARWLKTITRQGLGRGAFEALRADPDNIFDSAEFA
CCCHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCHHHC
GSPILIAGDNFGCGSSREHAAWALLDMGVKAVIAPSFSDIFSGNAFKNGILTVVLPQEAI
CCCEEEECCCCCCCCCCCHHHHHHHHHCCCEEECCCHHHHHCCCCCCCCCEEEEECHHHH
DRLMEVAQTDPVSIDLEAQTVTTPFQDRFSFEIDPFRKHCLANGLDEVGLTMARGDAIAT
HHHHHHHCCCCCEEEEECCEECCCCCCCCCCCCCHHHHHHHHCCHHHHCEEEECCCCHHH
HEARMRESLPFLAKGTDAVAAA
HHHHHHHCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA