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The map label for this gene is glnA [H]

Identifier: 87199130

GI number: 87199130

Start: 1155575

End: 1156984

Strand: Reverse

Name: glnA [H]

Synonym: Saro_1108

Alternate gene names: 87199130

Gene position: 1156984-1155575 (Counterclockwise)

Preceding gene: 87199131

Following gene: 87199129

Centisome position: 32.49

GC content: 63.19

Gene sequence:

>1410_bases
ATGGCAACTGCAAAGGACATCCTCGCCCGCATCAAGGACGAGGAAATCGAATGGGTCGATCTCCGTTTCACCGACCCGAA
GGGCAAGTGGCAGCACCTGACCATGGTGTCGACCGTCCTCGGCGAGGATGAACTCGAGGACGGCCTCATGTTCGACGGTT
CGTCGATCGAAGGCTGGAAGGCCATCAACGAGTCGGACATGATCCTCAAGCCCGACCTCGACGCGGTCTACGTCGATCCG
TTCTCGGCCAGCCCGATGCTGATCATCTGCTGCGACATCGTCGAGCCTTCGACCGGCGAACTCTACGCCCGCGACCCGCG
CTCGACCGCGAAGCGCGCCGAAGCCTTCGTCAAGACGGCCGGCCTTGGCGACACCGTCTACGTCGGCCCGGAAGCAGAGT
TCTTCCTCTTCGACGACGTCAAGTTCTACGACGGCTATGACGGCAACGGTTTCCGCATCGACGACATCGAGCTGCCGACC
AACTCGAACAAGGACATCGAAGGCGGCAACCTCGCCCACCGTCCGCGCGCCAAGGGCGGCTACTTCCCCGTCGCGCCGGT
CGACAGCTGCGTCGACATCCGCGGCGAGATGGTCCGCACCATGCTCGACATGGGCCTGCCCTGCGACAAGCACCACCACG
AAGTGGCCTCGGCCCAGCACGAGCTCGGCCTGACCTTCGGCACGCTCGTCCAGACCGCCGACCGCATGCAGGTCTACAAG
TACGTCGTGCAGCAGGTCGCCCATGCCTATGGCAAGACCGCCACGTTCATGCCGAAGCCGATCAAGGGCGACAACGGCTC
GGGCATGCACACCCACATCTCGATCTGGGATAGCGGCAAGCCCCTGTTCGCCGGTAATGGCTATGCCGGCCTGTCGGACA
TGTGCCTGTACTTCATCGGCGGCGTGATCAAGCACGCCAAGGCGCTGAACGCCTTCACCAACCCGACCACCAACAGCTAC
AAGCGCCTGGTGCCGGGCTACGAAGCCCCCGTTCTGCTGGCCTACTCGGCCCGCAACCGTTCGGCTTCGTGCCGCATCCC
CTATGGCGCTGGCGCCAAGGCAAAGCGCGTGGAATTCCGTTTCCCCGACGCGATGGCCAACCCCTACCTGTGCTACGCTG
CGCTGCTCATGGCCGGCATCGACGGGATCAAGAACAAGATCCATCCGGGCGAAGCCATGGACAAGAACCTCTACGACCTG
CCGCCGGCAGAGCTCGCCGAAGTTCCGACCGTGTGCGGATCGCTCCGTGAAGCGCTGGAAAGCCTCGAAGCCGACCACGA
CTTCCTGCTGCAGGGCGGCGTGTTCACCAAGGAACAGATCGAAAGCTACATCGAACTGAAGTGGCCGGAAGTGCTGCGTT
GGGAAACCACGCCGTCGGCCGTCGAGTTCGACATGTACTACTCGCTCTGA

Upstream 100 bases:

>100_bases
ATCAGATGGAGGGTGGCCCGTGGACGGGCGAGCCTCCAGAAACACAACCACCCTGTTACCCCCCGCAGCTCTCCACCGGG
GGCAAGAAGAAGGACCAGCG

Downstream 100 bases:

>100_bases
TCCTTCGGGACCTGCGAAACAGGAGGGGCGTCCGGGAAACCGGGCGCCCTTTCCTTTTGATGTCCGCAAAGCCCGGCATC
CACATGCCCGCCACAGCTGG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MATAKDILARIKDEEIEWVDLRFTDPKGKWQHLTMVSTVLGEDELEDGLMFDGSSIEGWKAINESDMILKPDLDAVYVDP
FSASPMLIICCDIVEPSTGELYARDPRSTAKRAEAFVKTAGLGDTVYVGPEAEFFLFDDVKFYDGYDGNGFRIDDIELPT
NSNKDIEGGNLAHRPRAKGGYFPVAPVDSCVDIRGEMVRTMLDMGLPCDKHHHEVASAQHELGLTFGTLVQTADRMQVYK
YVVQQVAHAYGKTATFMPKPIKGDNGSGMHTHISIWDSGKPLFAGNGYAGLSDMCLYFIGGVIKHAKALNAFTNPTTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYGAGAKAKRVEFRFPDAMANPYLCYAALLMAGIDGIKNKIHPGEAMDKNLYDL
PPAELAEVPTVCGSLREALESLEADHDFLLQGGVFTKEQIESYIELKWPEVLRWETTPSAVEFDMYYSL

Sequences:

>Translated_469_residues
MATAKDILARIKDEEIEWVDLRFTDPKGKWQHLTMVSTVLGEDELEDGLMFDGSSIEGWKAINESDMILKPDLDAVYVDP
FSASPMLIICCDIVEPSTGELYARDPRSTAKRAEAFVKTAGLGDTVYVGPEAEFFLFDDVKFYDGYDGNGFRIDDIELPT
NSNKDIEGGNLAHRPRAKGGYFPVAPVDSCVDIRGEMVRTMLDMGLPCDKHHHEVASAQHELGLTFGTLVQTADRMQVYK
YVVQQVAHAYGKTATFMPKPIKGDNGSGMHTHISIWDSGKPLFAGNGYAGLSDMCLYFIGGVIKHAKALNAFTNPTTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYGAGAKAKRVEFRFPDAMANPYLCYAALLMAGIDGIKNKIHPGEAMDKNLYDL
PPAELAEVPTVCGSLREALESLEADHDFLLQGGVFTKEQIESYIELKWPEVLRWETTPSAVEFDMYYSL
>Mature_468_residues
ATAKDILARIKDEEIEWVDLRFTDPKGKWQHLTMVSTVLGEDELEDGLMFDGSSIEGWKAINESDMILKPDLDAVYVDPF
SASPMLIICCDIVEPSTGELYARDPRSTAKRAEAFVKTAGLGDTVYVGPEAEFFLFDDVKFYDGYDGNGFRIDDIELPTN
SNKDIEGGNLAHRPRAKGGYFPVAPVDSCVDIRGEMVRTMLDMGLPCDKHHHEVASAQHELGLTFGTLVQTADRMQVYKY
VVQQVAHAYGKTATFMPKPIKGDNGSGMHTHISIWDSGKPLFAGNGYAGLSDMCLYFIGGVIKHAKALNAFTNPTTNSYK
RLVPGYEAPVLLAYSARNRSASCRIPYGAGAKAKRVEFRFPDAMANPYLCYAALLMAGIDGIKNKIHPGEAMDKNLYDLP
PAELAEVPTVCGSLREALESLEADHDFLLQGGVFTKEQIESYIELKWPEVLRWETTPSAVEFDMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=61.1111111111111, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI87081870, Length=396, Percent_Identity=24.7474747474747, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281360453, Length=338, Percent_Identity=26.6272189349112, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136208, Length=338, Percent_Identity=26.6272189349112, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24580558, Length=338, Percent_Identity=26.6272189349112, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51908; Mature: 51777

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAKDILARIKDEEIEWVDLRFTDPKGKWQHLTMVSTVLGEDELEDGLMFDGSSIEGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCHHHCCCEEECCCCCCCCC
AINESDMILKPDLDAVYVDPFSASPMLIICCDIVEPSTGELYARDPRSTAKRAEAFVKTA
CCCCCCCEECCCCCEEEECCCCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHC
GLGDTVYVGPEAEFFLFDDVKFYDGYDGNGFRIDDIELPTNSNKDIEGGNLAHRPRAKGG
CCCCEEEECCCCCEEEEECEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
YFPVAPVDSCVDIRGEMVRTMLDMGLPCDKHHHEVASAQHELGLTFGTLVQTADRMQVYK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YVVQQVAHAYGKTATFMPKPIKGDNGSGMHTHISIWDSGKPLFAGNGYAGLSDMCLYFIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHH
GVIKHAKALNAFTNPTTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYGAGAKAKRVEFR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEEEE
FPDAMANPYLCYAALLMAGIDGIKNKIHPGEAMDKNLYDLPPAELAEVPTVCGSLREALE
CCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
SLEADHDFLLQGGVFTKEQIESYIELKWPEVLRWETTPSAVEFDMYYSL
HHCCCCCEEEECCCCCHHHHHHHHHCCCCHHEEECCCCCEEEEEEEECC
>Mature Secondary Structure 
ATAKDILARIKDEEIEWVDLRFTDPKGKWQHLTMVSTVLGEDELEDGLMFDGSSIEGWK
CCHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCHHHCCCEEECCCCCCCCC
AINESDMILKPDLDAVYVDPFSASPMLIICCDIVEPSTGELYARDPRSTAKRAEAFVKTA
CCCCCCCEECCCCCEEEECCCCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHC
GLGDTVYVGPEAEFFLFDDVKFYDGYDGNGFRIDDIELPTNSNKDIEGGNLAHRPRAKGG
CCCCEEEECCCCCEEEEECEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
YFPVAPVDSCVDIRGEMVRTMLDMGLPCDKHHHEVASAQHELGLTFGTLVQTADRMQVYK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YVVQQVAHAYGKTATFMPKPIKGDNGSGMHTHISIWDSGKPLFAGNGYAGLSDMCLYFIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHH
GVIKHAKALNAFTNPTTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYGAGAKAKRVEFR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEEEE
FPDAMANPYLCYAALLMAGIDGIKNKIHPGEAMDKNLYDLPPAELAEVPTVCGSLREALE
CCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
SLEADHDFLLQGGVFTKEQIESYIELKWPEVLRWETTPSAVEFDMYYSL
HHCCCCCEEEECCCCCHHHHHHHHHCCCCHHEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]