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The map label for this gene is gltX

Identifier: 87198989

GI number: 87198989

Start: 1013326

End: 1014654

Strand: Reverse

Name: gltX

Synonym: Saro_0967

Alternate gene names: 87198989

Gene position: 1014654-1013326 (Counterclockwise)

Preceding gene: 87198990

Following gene: 87198987

Centisome position: 28.49

GC content: 68.77

Gene sequence:

>1329_bases
ATGGCCACCGTAACCCGCTTCGCGCCTTCGCCCACCGGCAAGCTCCATGTCGGCAACGTCCGCACCGCCCTCCACAACTG
GCTGCTGGCGAAGAAGACCGGCGGCCGCTTCCTCCTGCGCATCGACGATACCGACGCCGAGCGCAGCCGCGAGGAATACG
TCGAATCCATCCGCGCGGATCTCCAGTGGCTGGGCCTCATCCCCGATGGCGAGGAACGCCAGTCGCTGCGCACCGAACTC
TACGAGCGCGAATTTCAGCGTCTGGTCGAGGCGGGACGCATCTACCGGGCCTACGAAACCGCGCAGGAACTCGATCTCAA
GCGCAAGATCCTGCTCGGTCGCGGCCTGCCCCCGATCTATGACCGCGCCGCGCTGAAGCTGACCGAGGCGGACCACGCCG
CAAAGGCTGCGGCAGGCGAAAGGCCGCACTGGCGCTTCCTCCTCGACCACGACCAGCCGATCACCTGGGACGACGGCATT
CGCGGGCCCCAGAGCTTCGACCCCCGGCAGATGTCCGACCCCGTCATCCGCCGCGCGGACGGCTCGTGGCTCTACATGCT
GCCCTCGGCCATCGACGACATCGCCATGGGCATCACCGACGTCCTGCGCGGCGAGGACCACGTCTCGAACACCGCAACGC
AGATCCAGATGTTCACGGCGCTGGGTGCAGAACCGCCGCGTTTCGCCCACGAAGCCCTGCTGACGGGCAGCGAAGGCAAG
CTCTCCAAGCGCCTCGGTGCGCTCGGCATGGCCGACTTTCGCGAACAGGGCATCGAACCCGAGGCCATCGTCGCGCTTCT
CGCCCGCCTCGGCACTTCGGACCCGGTGGACGCGGCGCTCGATGCCGCGGCCCTTGCAACCAGCTTCGACCTGTCCCGCT
TCGGCCGCGCACCTGCCCGTTTCGACGAGGCCGATCTCCACCGCGTCAACGCGCAGATCGTCCATCGCCTGCCTTATGCC
CGCGTCGCACACCTCCTGCCACAGGGCATGGGCGAGGCCGCATGGGAAGCGATCCGCCCCAACCTCGCCCACATCGACGA
GGCGCGCGACTGGTGGAACGTGGTGACGGGGCCCGTCACCGCCCCGACCTTCGACGATGAAACGCGCGCCTTCCTCGCGC
AGGCCGCCAAAACCGCCGCCTCGCTGGATTGGTCCGCCGATCCGTGGCGCGCCCTCACCGCGACCCTCAAGGACGGCACC
GGCCGCAAGGGCAAGGCCCTGTTCCTCCCCCTGCGTCAGGCCCTTACCGGCCACGACCACGGCCCGGAAATGGCCGCCCT
CCTGCCTTTGATCGCGCAGGACGAGGCTGTACGCCGCCTGTCCGCATGA

Upstream 100 bases:

>100_bases
CCGCCACGATGCGCTGTACGAGGCGACGCTGCACGCGGTTGCCCTTATCCCCGCGGCCTGACCCTTCCCCAAACGCCCCT
GGCGCGATAGGGCGGCGCGC

Downstream 100 bases:

>100_bases
AATGAAAAGGGGCGGCGGCCCATCGGCCCCGCCCCTTTTCATTTCAACGGGTAATCCCGATCAGGCGGCGACAACGCTCG
CCTTGCGGATCACGCCCGGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MATVTRFAPSPTGKLHVGNVRTALHNWLLAKKTGGRFLLRIDDTDAERSREEYVESIRADLQWLGLIPDGEERQSLRTEL
YEREFQRLVEAGRIYRAYETAQELDLKRKILLGRGLPPIYDRAALKLTEADHAAKAAAGERPHWRFLLDHDQPITWDDGI
RGPQSFDPRQMSDPVIRRADGSWLYMLPSAIDDIAMGITDVLRGEDHVSNTATQIQMFTALGAEPPRFAHEALLTGSEGK
LSKRLGALGMADFREQGIEPEAIVALLARLGTSDPVDAALDAAALATSFDLSRFGRAPARFDEADLHRVNAQIVHRLPYA
RVAHLLPQGMGEAAWEAIRPNLAHIDEARDWWNVVTGPVTAPTFDDETRAFLAQAAKTAASLDWSADPWRALTATLKDGT
GRKGKALFLPLRQALTGHDHGPEMAALLPLIAQDEAVRRLSA

Sequences:

>Translated_442_residues
MATVTRFAPSPTGKLHVGNVRTALHNWLLAKKTGGRFLLRIDDTDAERSREEYVESIRADLQWLGLIPDGEERQSLRTEL
YEREFQRLVEAGRIYRAYETAQELDLKRKILLGRGLPPIYDRAALKLTEADHAAKAAAGERPHWRFLLDHDQPITWDDGI
RGPQSFDPRQMSDPVIRRADGSWLYMLPSAIDDIAMGITDVLRGEDHVSNTATQIQMFTALGAEPPRFAHEALLTGSEGK
LSKRLGALGMADFREQGIEPEAIVALLARLGTSDPVDAALDAAALATSFDLSRFGRAPARFDEADLHRVNAQIVHRLPYA
RVAHLLPQGMGEAAWEAIRPNLAHIDEARDWWNVVTGPVTAPTFDDETRAFLAQAAKTAASLDWSADPWRALTATLKDGT
GRKGKALFLPLRQALTGHDHGPEMAALLPLIAQDEAVRRLSA
>Mature_441_residues
ATVTRFAPSPTGKLHVGNVRTALHNWLLAKKTGGRFLLRIDDTDAERSREEYVESIRADLQWLGLIPDGEERQSLRTELY
EREFQRLVEAGRIYRAYETAQELDLKRKILLGRGLPPIYDRAALKLTEADHAAKAAAGERPHWRFLLDHDQPITWDDGIR
GPQSFDPRQMSDPVIRRADGSWLYMLPSAIDDIAMGITDVLRGEDHVSNTATQIQMFTALGAEPPRFAHEALLTGSEGKL
SKRLGALGMADFREQGIEPEAIVALLARLGTSDPVDAALDAAALATSFDLSRFGRAPARFDEADLHRVNAQIVHRLPYAR
VAHLLPQGMGEAAWEAIRPNLAHIDEARDWWNVVTGPVTAPTFDDETRAFLAQAAKTAASLDWSADPWRALTATLKDGTG
RKGKALFLPLRQALTGHDHGPEMAALLPLIAQDEAVRRLSA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=329, Percent_Identity=34.3465045592705, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=33.3333333333333, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=31.4285714285714, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71993394, Length=310, Percent_Identity=38.0645161290323, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324540, Length=487, Percent_Identity=30.3901437371663, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324742, Length=113, Percent_Identity=38.0530973451327, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24665321, Length=477, Percent_Identity=31.2368972746331, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_NOVAD (Q2G9R1)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496246.1
- ProteinModelPortal:   Q2G9R1
- SMR:   Q2G9R1
- STRING:   Q2G9R1
- GeneID:   3915749
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0967
- NMPDR:   fig|48935.1.peg.3073
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BioCyc:   NARO279238:SARO_0967-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 48923; Mature: 48792

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVTRFAPSPTGKLHVGNVRTALHNWLLAKKTGGRFLLRIDDTDAERSREEYVESIRAD
CCCCCCCCCCCCCCEEECHHHHHHHHHHHEEECCCEEEEEECCCCHHHHHHHHHHHHHHC
LQWLGLIPDGEERQSLRTELYEREFQRLVEAGRIYRAYETAQELDLKRKILLGRGLPPIY
HHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DRAALKLTEADHAAKAAAGERPHWRFLLDHDQPITWDDGIRGPQSFDPRQMSDPVIRRAD
HHHHEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHCCCC
GSWLYMLPSAIDDIAMGITDVLRGEDHVSNTATQIQMFTALGAEPPRFAHEALLTGSEGK
CCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCH
LSKRLGALGMADFREQGIEPEAIVALLARLGTSDPVDAALDAAALATSFDLSRFGRAPAR
HHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
FDEADLHRVNAQIVHRLPYARVAHLLPQGMGEAAWEAIRPNLAHIDEARDWWNVVTGPVT
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
APTFDDETRAFLAQAAKTAASLDWSADPWRALTATLKDGTGRKGKALFLPLRQALTGHDH
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCC
GPEMAALLPLIAQDEAVRRLSA
CCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATVTRFAPSPTGKLHVGNVRTALHNWLLAKKTGGRFLLRIDDTDAERSREEYVESIRAD
CCCCCCCCCCCCCEEECHHHHHHHHHHHEEECCCEEEEEECCCCHHHHHHHHHHHHHHC
LQWLGLIPDGEERQSLRTELYEREFQRLVEAGRIYRAYETAQELDLKRKILLGRGLPPIY
HHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DRAALKLTEADHAAKAAAGERPHWRFLLDHDQPITWDDGIRGPQSFDPRQMSDPVIRRAD
HHHHEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHCCCC
GSWLYMLPSAIDDIAMGITDVLRGEDHVSNTATQIQMFTALGAEPPRFAHEALLTGSEGK
CCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCH
LSKRLGALGMADFREQGIEPEAIVALLARLGTSDPVDAALDAAALATSFDLSRFGRAPAR
HHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
FDEADLHRVNAQIVHRLPYARVAHLLPQGMGEAAWEAIRPNLAHIDEARDWWNVVTGPVT
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
APTFDDETRAFLAQAAKTAASLDWSADPWRALTATLKDGTGRKGKALFLPLRQALTGHDH
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCC
GPEMAALLPLIAQDEAVRRLSA
CCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA