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The map label for this gene is rotA [H]

Identifier: 87198859

GI number: 87198859

Start: 885781

End: 886479

Strand: Reverse

Name: rotA [H]

Synonym: Saro_0837

Alternate gene names: 87198859

Gene position: 886479-885781 (Counterclockwise)

Preceding gene: 87198860

Following gene: 87198858

Centisome position: 24.89

GC content: 67.95

Gene sequence:

>699_bases
ATGCTGAAACGCCGATTCGTCCTGCTCGCCCCGCTCGCGGTCCTGCTCGTGGCGGCCACCCCCGTGAAGAAGCGGCCCGT
TGTCGCGCGCCCGCCCGCTCCCGCCTATCCCGCACCATCCACCACCCCGTTGCCGGACAAGGTGCGCGTCGCGCTCGATA
CCGAGGCCGGGCGCATCGTGGTCGAAGTCGACGTTAAGCACGCGCCTGCCAGCGCCACCAACTTCGTGCGCTACGCCGAA
ACGAAGCGGTTCGACGGCACAGTACTCTACCGGGCGATGCATCTTCCATGGGGCGAACAGCCCAATGGCCTGATCCAGGG
CGGCACGCGCGGCGACCCGAAGCGCAACCTGCCGCCGGTCGCGCACGAGCCGACGAACGTCACCGGCATCCTTCACAAGG
CGGGTACGCTCTCGATGGCCCGCTATGCCCCTGGCACGGCGACCGGGGATTTCTCGATCATGGTTTCCGACCAGCCGGGG
CTCGACGCCCAGCCGGATTCAGCCGATCCCGAAGCGAAGGCAGGATACGCCGCGTTCGGACAGGTCGTGGAGGGCATGGA
CGTGGTGAAGGCCATCTTCGCTGCGCCGATTTCCGAAACCGAGGGCGAAGGGATCATGCGCGGGCAGATCCTCGCCAAGC
CGGTGAAGATCCTGACTGCCCGGCGCCTGCCCGAAGCCCCGCTGCCATCCGCACCCTGA

Upstream 100 bases:

>100_bases
ACTGGACGCAGGGCGGCACCCCATGCGCCCCGGCCCAGAACGCCAAACCTTCCGCCGCCGGACAGTAGCCATCGGCGCGG
GATCTGGCCATGCTGCGCCC

Downstream 100 bases:

>100_bases
AATGCGGCGTGCGCTGCCTGCCATCGTAGCTGCGCTCGCCCTCGCAGGGTGCGCGCAACCCCGTCTGCGACCGTTCGAGC
AGGTCCTGGCGGCACAGGAC

Product: cyclophilin type peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MLKRRFVLLAPLAVLLVAATPVKKRPVVARPPAPAYPAPSTTPLPDKVRVALDTEAGRIVVEVDVKHAPASATNFVRYAE
TKRFDGTVLYRAMHLPWGEQPNGLIQGGTRGDPKRNLPPVAHEPTNVTGILHKAGTLSMARYAPGTATGDFSIMVSDQPG
LDAQPDSADPEAKAGYAAFGQVVEGMDVVKAIFAAPISETEGEGIMRGQILAKPVKILTARRLPEAPLPSAP

Sequences:

>Translated_232_residues
MLKRRFVLLAPLAVLLVAATPVKKRPVVARPPAPAYPAPSTTPLPDKVRVALDTEAGRIVVEVDVKHAPASATNFVRYAE
TKRFDGTVLYRAMHLPWGEQPNGLIQGGTRGDPKRNLPPVAHEPTNVTGILHKAGTLSMARYAPGTATGDFSIMVSDQPG
LDAQPDSADPEAKAGYAAFGQVVEGMDVVKAIFAAPISETEGEGIMRGQILAKPVKILTARRLPEAPLPSAP
>Mature_232_residues
MLKRRFVLLAPLAVLLVAATPVKKRPVVARPPAPAYPAPSTTPLPDKVRVALDTEAGRIVVEVDVKHAPASATNFVRYAE
TKRFDGTVLYRAMHLPWGEQPNGLIQGGTRGDPKRNLPPVAHEPTNVTGILHKAGTLSMARYAPGTATGDFSIMVSDQPG
LDAQPDSADPEAKAGYAAFGQVVEGMDVVKAIFAAPISETEGEGIMRGQILAKPVKILTARRLPEAPLPSAP

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789763, Length=180, Percent_Identity=36.1111111111111, Blast_Score=84, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 24589; Mature: 24589

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRRFVLLAPLAVLLVAATPVKKRPVVARPPAPAYPAPSTTPLPDKVRVALDTEAGRIV
CCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEE
VEVDVKHAPASATNFVRYAETKRFDGTVLYRAMHLPWGEQPNGLIQGGTRGDPKRNLPPV
EEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCC
AHEPTNVTGILHKAGTLSMARYAPGTATGDFSIMVSDQPGLDAQPDSADPEAKAGYAAFG
CCCCCCEEEEEECCCCEEHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHCCHHHHH
QVVEGMDVVKAIFAAPISETEGEGIMRGQILAKPVKILTARRLPEAPLPSAP
HHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLKRRFVLLAPLAVLLVAATPVKKRPVVARPPAPAYPAPSTTPLPDKVRVALDTEAGRIV
CCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEE
VEVDVKHAPASATNFVRYAETKRFDGTVLYRAMHLPWGEQPNGLIQGGTRGDPKRNLPPV
EEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCC
AHEPTNVTGILHKAGTLSMARYAPGTATGDFSIMVSDQPGLDAQPDSADPEAKAGYAAFG
CCCCCCEEEEEECCCCEEHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHCCHHHHH
QVVEGMDVVKAIFAAPISETEGEGIMRGQILAKPVKILTARRLPEAPLPSAP
HHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]