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The map label for this gene is ynfL [C]

Identifier: 87198561

GI number: 87198561

Start: 586351

End: 587277

Strand: Reverse

Name: ynfL [C]

Synonym: Saro_0537

Alternate gene names: 87198561

Gene position: 587277-586351 (Counterclockwise)

Preceding gene: 87198569

Following gene: 87198557

Centisome position: 16.49

GC content: 64.4

Gene sequence:

>927_bases
ATGGACCTCAGGCAACTTCGACAGTTTCTCGCGGTGGCCAATGCCCGCAGTTTTTCGCGCGCAGCGGAAACGCTGAACAT
CGCCCAGCCGCCCTTGAGCCGGACGATCCGGCACCTGGAGGAGGAGATCGGCGGCGCCCTGTTCGACCGCACCGCCCGCC
CGCTGGCGATGACCCCGCTCGGGCAGTTGCTGCACGAGCAGGCGCGACAGATCCTCGATCGCATGGGCGACATGCACGAC
ATGATGAAGGCCGCCGCCGCGACCGAGCGCCGGCGCTTCACCATCGGCTTCGTCGCTTCGACGATCTATGCGAGGTTACC
CGAACTCATTCGCGCCTTTCGCAAGCGCGCGCCCGACGTGGAACTGACACTCGTCGAAAGTTCGACGCTCGAACAACTGG
CCGCCCTCAAGGATGGCCGCATCGATGTCGGCTTCGGTCGCATCCGGTTCGAGGACCCGGCAATCAAGCGCATCATCCTG
CGCAACGAACGCCTTGCCGTAGCCTTGCCGCGTGACCATCCTCTTGCGCTCGACGATCGGCCACTCACCCTGAAGCAGGT
CGCTGCCGAGCCGCAGATCCTCTATCCGCGCGCTCCGCGTCCGAGCTATGCCGACCAGGTCATCTCGCTGTTCCGCGATC
ACGGCCTCGAACCCAGAATTGCCCACGAAGCGCGCGAACTGCAGATCGCCATCGGCCTGGTCGCGGCCGAGGAAGGCCTT
GCGATCATCCCGGAGTCCATCAGCCGTTCAAGAAGCCACGATGTCGTCTACCGGGAGCTGGCCGAAGCGGCGACTTCTCC
GATCATCATGAGCCACCGGATGAACGACAGCTCTGCCGAACTCCGGCTGATGGCGCAGGTTATTGCAAGCGAATATGCAG
CCTGGGGGTACCCCGTACCCGAAATCCTCGCCCACTCCGCCCCCTGA

Upstream 100 bases:

>100_bases
CGCCTCTCCAGAATCAGGCAATTTTGCTGGAGAATATCGGGACCGAAACGCCCCATCAAAGATCATTGCGGTCAATTGAT
CATACCCTTAGAGTATAACG

Downstream 100 bases:

>100_bases
TCCCGGAATGCCGAAACTGGAACCGGGTGTTCGGAGAACCGGGGCGCGCATCCTGTCCGGTCATCCCCGGACAACACACC
TGAAGCCGATGTGGGAGGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORFR1 [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MDLRQLRQFLAVANARSFSRAAETLNIAQPPLSRTIRHLEEEIGGALFDRTARPLAMTPLGQLLHEQARQILDRMGDMHD
MMKAAAATERRRFTIGFVASTIYARLPELIRAFRKRAPDVELTLVESSTLEQLAALKDGRIDVGFGRIRFEDPAIKRIIL
RNERLAVALPRDHPLALDDRPLTLKQVAAEPQILYPRAPRPSYADQVISLFRDHGLEPRIAHEARELQIAIGLVAAEEGL
AIIPESISRSRSHDVVYRELAEAATSPIIMSHRMNDSSAELRLMAQVIASEYAAWGYPVPEILAHSAP

Sequences:

>Translated_308_residues
MDLRQLRQFLAVANARSFSRAAETLNIAQPPLSRTIRHLEEEIGGALFDRTARPLAMTPLGQLLHEQARQILDRMGDMHD
MMKAAAATERRRFTIGFVASTIYARLPELIRAFRKRAPDVELTLVESSTLEQLAALKDGRIDVGFGRIRFEDPAIKRIIL
RNERLAVALPRDHPLALDDRPLTLKQVAAEPQILYPRAPRPSYADQVISLFRDHGLEPRIAHEARELQIAIGLVAAEEGL
AIIPESISRSRSHDVVYRELAEAATSPIIMSHRMNDSSAELRLMAQVIASEYAAWGYPVPEILAHSAP
>Mature_308_residues
MDLRQLRQFLAVANARSFSRAAETLNIAQPPLSRTIRHLEEEIGGALFDRTARPLAMTPLGQLLHEQARQILDRMGDMHD
MMKAAAATERRRFTIGFVASTIYARLPELIRAFRKRAPDVELTLVESSTLEQLAALKDGRIDVGFGRIRFEDPAIKRIIL
RNERLAVALPRDHPLALDDRPLTLKQVAAEPQILYPRAPRPSYADQVISLFRDHGLEPRIAHEARELQIAIGLVAAEEGL
AIIPESISRSRSHDVVYRELAEAATSPIIMSHRMNDSSAELRLMAQVIASEYAAWGYPVPEILAHSAP

Specific function: Probable positive regulator of the cat1 operon which encode enzymes responsible for the degradation of catechol to acetyl-CoA via the beta-ketoadipate pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=263, Percent_Identity=34.6007604562738, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788887, Length=283, Percent_Identity=32.8621908127208, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI2367136, Length=264, Percent_Identity=28.7878787878788, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790399, Length=259, Percent_Identity=28.957528957529, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=25.7142857142857, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=28.1632653061224, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081904, Length=203, Percent_Identity=30.0492610837438, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787806, Length=264, Percent_Identity=28.030303030303, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790262, Length=268, Percent_Identity=26.4925373134328, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787601, Length=251, Percent_Identity=24.3027888446215, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=25.2788104089219, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788296, Length=198, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=188, Percent_Identity=30.3191489361702, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790783, Length=147, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789204, Length=250, Percent_Identity=28.8, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788481, Length=204, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788297, Length=260, Percent_Identity=26.5384615384615, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789506, Length=148, Percent_Identity=25.6756756756757, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=143, Percent_Identity=29.3706293706294, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=258, Percent_Identity=25.968992248062, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34477; Mature: 34477

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLRQFLAVANARSFSRAAETLNIAQPPLSRTIRHLEEEIGGALFDRTARPLAMTPL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCCCCCHHHH
GQLLHEQARQILDRMGDMHDMMKAAAATERRRFTIGFVASTIYARLPELIRAFRKRAPDV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELTLVESSTLEQLAALKDGRIDVGFGRIRFEDPAIKRIILRNERLAVALPRDHPLALDDR
EEEEECHHHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCC
PLTLKQVAAEPQILYPRAPRPSYADQVISLFRDHGLEPRIAHEARELQIAIGLVAAEEGL
CCHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCC
AIIPESISRSRSHDVVYRELAEAATSPIIMSHRMNDSSAELRLMAQVIASEYAAWGYPVP
EECCHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHH
EILAHSAP
HHHCCCCC
>Mature Secondary Structure
MDLRQLRQFLAVANARSFSRAAETLNIAQPPLSRTIRHLEEEIGGALFDRTARPLAMTPL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCCCCCHHHH
GQLLHEQARQILDRMGDMHDMMKAAAATERRRFTIGFVASTIYARLPELIRAFRKRAPDV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELTLVESSTLEQLAALKDGRIDVGFGRIRFEDPAIKRIILRNERLAVALPRDHPLALDDR
EEEEECHHHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCC
PLTLKQVAAEPQILYPRAPRPSYADQVISLFRDHGLEPRIAHEARELQIAIGLVAAEEGL
CCHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCC
AIIPESISRSRSHDVVYRELAEAATSPIIMSHRMNDSSAELRLMAQVIASEYAAWGYPVP
EECCHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHH
EILAHSAP
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473520 [H]