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The map label for this gene is paaF [C]

Identifier: 87198542

GI number: 87198542

Start: 558851

End: 559693

Strand: Reverse

Name: paaF [C]

Synonym: Saro_0518

Alternate gene names: 87198542

Gene position: 559693-558851 (Counterclockwise)

Preceding gene: 87198545

Following gene: 87198541

Centisome position: 15.71

GC content: 63.58

Gene sequence:

>843_bases
ATGGATGCCGTGGACGGAGTGAGACTCGAGGAAGGCGACGCAATCGACTGGATCGTCTTCGATCGCCCGCAAGCTGCGAA
CAGCTTCTCCGCCACCCTGCTGGAACAATTCTCGGCGCTGGTTAAGGACCGACAGGCCAATGGCGCACCGGTTCTCGGGA
TTCGCGGGAGCGGTCGCGGTTTCAGCTCGGGCATGGATCTGGGCGAATACAATGCCACGAGCGGCCCCACCAGCGACGTA
CTGCGGCTCTCTTCCTATGTCGAACGCTGGCTGGACCTTTGGCGCCACCCGAAGCCGGTGATCGTCGCGGTCCACGGCTA
TTGCATCGGCGTTGCCGCGCAGCTTGCGTCGTTCGCGGATATCCTCGTGGTGGCCGAAGACGCGATGATAAGCGAGCCCA
CCATTCCGATCGGAGGAGGCTTCATCGCCCCCACCTGGGTGAGCCACGTCGGCTCTCGCCACGCGAAGGAGTTCGCCTTT
CTGCCTGGCAACCGGATCGACGGCCGCATGGCGGCAGCTTGGGGCTGGGCCAATTGCGCGGTCCCTGCTTCGGAAGTGAT
CGCGTGTTGCGAGAGTCTGGCCCAGCGGATGAAGCTTATGCCGCCTGCCGTCCTGGCCATGAAGAAGCGGTCGATCAACC
GGGCGATGGAGGCAGCCGGATTTCACGCAGCGGCTTCGGCAATCGCCGAAAGCGACGCCCTTCTTCATCTCGAACCCGAG
GTAACGGCGATCCGCAACCGCCTGCGGACCGAGGATCTCAAGGCTGTCGTCGGCTCATATGCCGGCGAAAGCTCCCAGGA
AATATTCCAGCGCCACGGCGGTCATCGACCCGACGCTTCCTGA

Upstream 100 bases:

>100_bases
CCCCAACTTCAACCTAATTCATTTTAGGTTTGCCCTAATTTAAGCCAAGTGTAAATAGGCCATACAGACTGCTCGCAACG
ACCGAAGCAGGGAGAGAGAG

Downstream 100 bases:

>100_bases
GCAACAATTTGGTTGCCCGCGCAGACTAATCATGATTAGGTTTGAGTTTACACTCCGGCAAGAGCCCCCGCTCTTGCGCG
ATACAGGAAGCGGGATGACG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MDAVDGVRLEEGDAIDWIVFDRPQAANSFSATLLEQFSALVKDRQANGAPVLGIRGSGRGFSSGMDLGEYNATSGPTSDV
LRLSSYVERWLDLWRHPKPVIVAVHGYCIGVAAQLASFADILVVAEDAMISEPTIPIGGGFIAPTWVSHVGSRHAKEFAF
LPGNRIDGRMAAAWGWANCAVPASEVIACCESLAQRMKLMPPAVLAMKKRSINRAMEAAGFHAAASAIAESDALLHLEPE
VTAIRNRLRTEDLKAVVGSYAGESSQEIFQRHGGHRPDAS

Sequences:

>Translated_280_residues
MDAVDGVRLEEGDAIDWIVFDRPQAANSFSATLLEQFSALVKDRQANGAPVLGIRGSGRGFSSGMDLGEYNATSGPTSDV
LRLSSYVERWLDLWRHPKPVIVAVHGYCIGVAAQLASFADILVVAEDAMISEPTIPIGGGFIAPTWVSHVGSRHAKEFAF
LPGNRIDGRMAAAWGWANCAVPASEVIACCESLAQRMKLMPPAVLAMKKRSINRAMEAAGFHAAASAIAESDALLHLEPE
VTAIRNRLRTEDLKAVVGSYAGESSQEIFQRHGGHRPDAS
>Mature_280_residues
MDAVDGVRLEEGDAIDWIVFDRPQAANSFSATLLEQFSALVKDRQANGAPVLGIRGSGRGFSSGMDLGEYNATSGPTSDV
LRLSSYVERWLDLWRHPKPVIVAVHGYCIGVAAQLASFADILVVAEDAMISEPTIPIGGGFIAPTWVSHVGSRHAKEFAF
LPGNRIDGRMAAAWGWANCAVPASEVIACCESLAQRMKLMPPAVLAMKKRSINRAMEAAGFHAAASAIAESDALLHLEPE
VTAIRNRLRTEDLKAVVGSYAGESSQEIFQRHGGHRPDAS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=219, Percent_Identity=29.6803652968037, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787659, Length=215, Percent_Identity=27.906976744186, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=216, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=213, Percent_Identity=28.169014084507, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=226, Percent_Identity=22.5663716814159, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20129971, Length=213, Percent_Identity=28.6384976525822, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24653477, Length=213, Percent_Identity=28.6384976525822, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29994; Mature: 29994

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVDGVRLEEGDAIDWIVFDRPQAANSFSATLLEQFSALVKDRQANGAPVLGIRGSGRG
CCCCCCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
FSSGMDLGEYNATSGPTSDVLRLSSYVERWLDLWRHPKPVIVAVHGYCIGVAAQLASFAD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHH
ILVVAEDAMISEPTIPIGGGFIAPTWVSHVGSRHAKEFAFLPGNRIDGRMAAAWGWANCA
HHHEECCHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHEEECCCCCCCCCEEHHCCCCCCC
VPASEVIACCESLAQRMKLMPPAVLAMKKRSINRAMEAAGFHAAASAIAESDALLHLEPE
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCH
VTAIRNRLRTEDLKAVVGSYAGESSQEIFQRHGGHRPDAS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDAVDGVRLEEGDAIDWIVFDRPQAANSFSATLLEQFSALVKDRQANGAPVLGIRGSGRG
CCCCCCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
FSSGMDLGEYNATSGPTSDVLRLSSYVERWLDLWRHPKPVIVAVHGYCIGVAAQLASFAD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHH
ILVVAEDAMISEPTIPIGGGFIAPTWVSHVGSRHAKEFAFLPGNRIDGRMAAAWGWANCA
HHHEECCHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHEEECCCCCCCCCEEHHCCCCCCC
VPASEVIACCESLAQRMKLMPPAVLAMKKRSINRAMEAAGFHAAASAIAESDALLHLEPE
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCH
VTAIRNRLRTEDLKAVVGSYAGESSQEIFQRHGGHRPDAS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA