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The map label for this gene is mltB [H]

Identifier: 86751619

GI number: 86751619

Start: 5108270

End: 5109664

Strand: Direct

Name: mltB [H]

Synonym: RPB_4521

Alternate gene names: 86751619

Gene position: 5108270-5109664 (Clockwise)

Preceding gene: 86751617

Following gene: 86751620

Centisome position: 95.81

GC content: 67.81

Gene sequence:

>1395_bases
ATGACGGAAACTGGCAACAGGCTAGCGCGGGTCGCAGGGATGGCCGTACTCGGCGCCGTGCTGCACACGGGCGCCGCGCA
GGCGCAGAACCCGTTCGACGTGTTCAATCTGTTCGGCGGCAAGAGCGCCGCGCCGAATGCGCCGCAGCCCGCCAATGTCG
GCCCGCAAAAGCCCGCTTCGCAGCAAGGCAGCAGCGCTGCGCCGCCGTGGAGCGGCGAGGACGGCGGCTCCGGCCATCCG
ACGATGACCGCGCAGGCGATCCGCGAGGCCGCCGCCAATTTCGACAATTGCGTCGCCGGCTTCTGGCCGGACGCTCAGCG
CCGCGGCATCTCGCGCGACAGCTTCGCGCGCTTCACCGCGGGGCTGTCGCCCGATCTGCGGCTGATGGACCTGATGGATT
CGCAGCCGGAATTCACCAAGGCGATCTGGGAGTATCTCGACATCCTGGTGAACGACAACAGGATGGCGCGGGGCCGCGAG
ATCCTGGCGCAATACAAGCCGCAATTCGACGCGGTGGAGCGCGCGTATGGCGTCGACCGCTACATCATTGCGTCGATCTG
GGGCATCGAGTCGAACTACTCGACCCAGATGGGCGACCGCTATGTGCTGAACTCGACCGCGACGCTGGCCTGCGTCGGCC
GCCGCCAAAACTACTTCAAGGACGAATTCCTCACCGCGTTGGAGATCCTGCATCGCGGCGATCTGCGTCCCGAGCAGTTG
CGCGGCTCGTGGGCCGGCGCGTTCGGCCCGACGCAGTTCATGCCGACCGCGTTCAAGCGCTTCGCCGTCGACGCCGACGG
CGACGGCCGCCGCGACGTCGTCGACAATCCCGCCGACCTGATCGCCTCGACCGCCAACAACCTCAAGAAGGACGGCTGGC
AGAGCGGCCAGACCTGGGGCTACGAGGTCGCGGTGCCGCGCGGCTTCGACTACATGAAGGCCGACCGCAAGCAGATGCTG
ACGCTGGCGCGATGGGAAGAGCTCGGCGTCCGCCGCCCGAACGGCCAGCCGTTCCCGCGCGGCGGCGAAAAGGCCTATCT
GCTGGCGCCCGCCGGCGCCGAAGGCCCCGGCTTCCTGATGCTGACCAATTTCCGCGTCATCATGAAGTACAACCCCGCCG
AAGCCTATGCGCTGGCGATCGGCCATTTCGCCGACCGCCTGCGCGGCGGTCCGCCCTTCGTCCAGCCCTGGCCGCGCCAC
GAACGCGTGCTGTCCCGCAGCGAACGCCTCGAACTGCAACAACTCCTCGCCCAACGCGGCTTCTACCGCGGCACCCCCGA
CGGCCAATTCGGCAGCCTGACGCGGGAAGGACTCCGGAATTTCCAGGCGTCCATTGGGGCCCCGGCGGATGGATTTGCCA
CGGGGGAGATGCTGGAGAGGTTGAGAGGAAGGTAG

Upstream 100 bases:

>100_bases
CACCGCCGACGCCAAAACAACGCCTTGTTAAGCTGATCGAAACCATGACGAGGCCTCCTGACCGCGGGGGCTCTATCGAG
CAAGCGGGATCGGACCGAGC

Downstream 100 bases:

>100_bases
CATCCCACGCCGCTGGAAAATTCCCACAACCTCTGGTTATACTGGGCCACGAGATTTTTGAACCACTCCATTTCATAACT
CGGAATTATGATCGTGCGCG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTETGNRLARVAGMAVLGAVLHTGAAQAQNPFDVFNLFGGKSAAPNAPQPANVGPQKPASQQGSSAAPPWSGEDGGSGHP
TMTAQAIREAAANFDNCVAGFWPDAQRRGISRDSFARFTAGLSPDLRLMDLMDSQPEFTKAIWEYLDILVNDNRMARGRE
ILAQYKPQFDAVERAYGVDRYIIASIWGIESNYSTQMGDRYVLNSTATLACVGRRQNYFKDEFLTALEILHRGDLRPEQL
RGSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLKKDGWQSGQTWGYEVAVPRGFDYMKADRKQML
TLARWEELGVRRPNGQPFPRGGEKAYLLAPAGAEGPGFLMLTNFRVIMKYNPAEAYALAIGHFADRLRGGPPFVQPWPRH
ERVLSRSERLELQQLLAQRGFYRGTPDGQFGSLTREGLRNFQASIGAPADGFATGEMLERLRGR

Sequences:

>Translated_464_residues
MTETGNRLARVAGMAVLGAVLHTGAAQAQNPFDVFNLFGGKSAAPNAPQPANVGPQKPASQQGSSAAPPWSGEDGGSGHP
TMTAQAIREAAANFDNCVAGFWPDAQRRGISRDSFARFTAGLSPDLRLMDLMDSQPEFTKAIWEYLDILVNDNRMARGRE
ILAQYKPQFDAVERAYGVDRYIIASIWGIESNYSTQMGDRYVLNSTATLACVGRRQNYFKDEFLTALEILHRGDLRPEQL
RGSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLKKDGWQSGQTWGYEVAVPRGFDYMKADRKQML
TLARWEELGVRRPNGQPFPRGGEKAYLLAPAGAEGPGFLMLTNFRVIMKYNPAEAYALAIGHFADRLRGGPPFVQPWPRH
ERVLSRSERLELQQLLAQRGFYRGTPDGQFGSLTREGLRNFQASIGAPADGFATGEMLERLRGR
>Mature_463_residues
TETGNRLARVAGMAVLGAVLHTGAAQAQNPFDVFNLFGGKSAAPNAPQPANVGPQKPASQQGSSAAPPWSGEDGGSGHPT
MTAQAIREAAANFDNCVAGFWPDAQRRGISRDSFARFTAGLSPDLRLMDLMDSQPEFTKAIWEYLDILVNDNRMARGREI
LAQYKPQFDAVERAYGVDRYIIASIWGIESNYSTQMGDRYVLNSTATLACVGRRQNYFKDEFLTALEILHRGDLRPEQLR
GSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLKKDGWQSGQTWGYEVAVPRGFDYMKADRKQMLT
LARWEELGVRRPNGQPFPRGGEKAYLLAPAGAEGPGFLMLTNFRVIMKYNPAEAYALAIGHFADRLRGGPPFVQPWPRHE
RVLSRSERLELQQLLAQRGFYRGTPDGQFGSLTREGLRNFQASIGAPADGFATGEMLERLRGR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=268, Percent_Identity=29.8507462686567, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 51117; Mature: 50986

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETGNRLARVAGMAVLGAVLHTGAAQAQNPFDVFNLFGGKSAAPNAPQPANVGPQKPAS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QQGSSAAPPWSGEDGGSGHPTMTAQAIREAAANFDNCVAGFWPDAQRRGISRDSFARFTA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHCCCCHHHHHHHHC
GLSPDLRLMDLMDSQPEFTKAIWEYLDILVNDNRMARGREILAQYKPQFDAVERAYGVDR
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCH
YIIASIWGIESNYSTQMGDRYVLNSTATLACVGRRQNYFKDEFLTALEILHRGDLRPEQL
HHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RGSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLKKDGWQSGQTWG
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
YEVAVPRGFDYMKADRKQMLTLARWEELGVRRPNGQPFPRGGEKAYLLAPAGAEGPGFLM
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
LTNFRVIMKYNPAEAYALAIGHFADRLRGGPPFVQPWPRHERVLSRSERLELQQLLAQRG
EECEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
FYRGTPDGQFGSLTREGLRNFQASIGAPADGFATGEMLERLRGR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TETGNRLARVAGMAVLGAVLHTGAAQAQNPFDVFNLFGGKSAAPNAPQPANVGPQKPAS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QQGSSAAPPWSGEDGGSGHPTMTAQAIREAAANFDNCVAGFWPDAQRRGISRDSFARFTA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHCCCCHHHHHHHHC
GLSPDLRLMDLMDSQPEFTKAIWEYLDILVNDNRMARGREILAQYKPQFDAVERAYGVDR
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCH
YIIASIWGIESNYSTQMGDRYVLNSTATLACVGRRQNYFKDEFLTALEILHRGDLRPEQL
HHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RGSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLKKDGWQSGQTWG
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
YEVAVPRGFDYMKADRKQMLTLARWEELGVRRPNGQPFPRGGEKAYLLAPAGAEGPGFLM
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
LTNFRVIMKYNPAEAYALAIGHFADRLRGGPPFVQPWPRHERVLSRSERLELQQLLAQRG
EECEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
FYRGTPDGQFGSLTREGLRNFQASIGAPADGFATGEMLERLRGR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]