Click here to switch to the map view.

The map label for this gene is pgk [H]

Identifier: 86751566

GI number: 86751566

Start: 5059264

End: 5060460

Strand: Direct

Name: pgk [H]

Synonym: RPB_4468

Alternate gene names: 86751566

Gene position: 5059264-5060460 (Clockwise)

Preceding gene: 86751565

Following gene: 86751567

Centisome position: 94.89

GC content: 67.59

Gene sequence:

>1197_bases
ATGACCAAATCATTCCGCACCCTCGACGACGTCGACGTCAAAGGCCAACGCGTGCTGCTGCGCGTCGATCTCAACGTGCC
GATGGATTCCGGCAAGGTCACCGACACCACGCGGCTCGACCGTGTCGCGCCGACGATCACCGAGATCGCCGGCAAGGGCG
GCAAGGTGATCCTGCTGGCGCATTTCGGCCGGCCGAAGGGGCGGGACGACAAGAACTCGCTGAAGCCGGTCGCGGCCGCG
CTCGCGGACGTCATCAAGAAGCCGGTCGGCTTCGCCGAGGACTGTGTCGGCGAGCCCGCCGCGACAGCGATCGCGGCGAT
GCAGGACGGCGACATCCTCTGCCTCGAGAACACCCGGTTCCATCCTGAAGAGGAAAAGAACGATCCGACGTTCGTCGCCA
AGCTCGCCGAGCTCGGCGACATCTGGGTCAATGACGCGTTCTCGGCGGCGCACCGCGCCCACGCCTCCACCGAGGGCCTC
GGCCACAAGCTGCCGGCCTATGCGGGCCGCACCATGCAGGCCGAGCTGGTGGCGCTGAACAAGGCGCTGGAAGCGCCGAC
CAAGCCGGTGATCGCCATTGTCGGCGGCGCCAAGGTGTCGAGCAAGATCGACCTCCTGGAAAACCTCGTCACCAAGGTGC
AGGCGCTGGTGATCGGCGGCGGCATGGCCAACACCTTCCTGCACGCCCAGGGCTTCAAGGTCGGCAAATCGCTCTGCGAA
AAGGATCTGGCCGAGACCGCGCTGCGGATCATGAACAAGGCCGAGGCCGCCAGCTGCGCGATCATCCTGCCGGTCGACGC
CACCGTGGCGTATCATTTCGAGGCCAACGCGCCGTCCTTCGCCTACGGCCTCGACGCGATTCCGGACGACGCCATGATCC
TCGACGTCGGCCCGCGCTCGATCGAGCGCATCCATGCGGCGATCGACGACGCCGCAACATTGGTCTGGAACGGCCCGGTC
GGCGCCTTCGAGCTGACGCCGTTCGACAAGGGCACGGTCGAGGCGGCCAGGCACGCCGCCCGCCGCACCAAGGCCGGGAT
GCTGATCTCGGTCGCCGGCGGCGGCGACACCGTGGCGGCGCTGAACCACGCCGGCGTGGCCGACGATTTCACCTACATCT
CGACCGCCGGCGGCGCCTTCCTCGAATGGATGGAAGGCAAGCCGCTGCCCGGCGTCGAGGTGCTGCGGGTGAAGTGA

Upstream 100 bases:

>100_bases
GCACGACGTGATGCGCTGCTGAACCGGGACCGTATCGTTTGTGGCGGTCCCGGCTCAGCGGTGCACCGCTTCGCGCTGCA
CCGCGTCCGGGACACATCAC

Downstream 100 bases:

>100_bases
GGCTCTGCGGCCGGCGCTCAGGCGCCGGCTTGCGGCCGGCCCATCCAAAGCCTATGTTGCGTTACATGTAACGCTTCCGA
CGGAGGCCGCGATGGATAAG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTKSFRTLDDVDVKGQRVLLRVDLNVPMDSGKVTDTTRLDRVAPTITEIAGKGGKVILLAHFGRPKGRDDKNSLKPVAAA
LADVIKKPVGFAEDCVGEPAATAIAAMQDGDILCLENTRFHPEEEKNDPTFVAKLAELGDIWVNDAFSAAHRAHASTEGL
GHKLPAYAGRTMQAELVALNKALEAPTKPVIAIVGGAKVSSKIDLLENLVTKVQALVIGGGMANTFLHAQGFKVGKSLCE
KDLAETALRIMNKAEAASCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRSIERIHAAIDDAATLVWNGPV
GAFELTPFDKGTVEAARHAARRTKAGMLISVAGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLRVK

Sequences:

>Translated_398_residues
MTKSFRTLDDVDVKGQRVLLRVDLNVPMDSGKVTDTTRLDRVAPTITEIAGKGGKVILLAHFGRPKGRDDKNSLKPVAAA
LADVIKKPVGFAEDCVGEPAATAIAAMQDGDILCLENTRFHPEEEKNDPTFVAKLAELGDIWVNDAFSAAHRAHASTEGL
GHKLPAYAGRTMQAELVALNKALEAPTKPVIAIVGGAKVSSKIDLLENLVTKVQALVIGGGMANTFLHAQGFKVGKSLCE
KDLAETALRIMNKAEAASCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRSIERIHAAIDDAATLVWNGPV
GAFELTPFDKGTVEAARHAARRTKAGMLISVAGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLRVK
>Mature_397_residues
TKSFRTLDDVDVKGQRVLLRVDLNVPMDSGKVTDTTRLDRVAPTITEIAGKGGKVILLAHFGRPKGRDDKNSLKPVAAAL
ADVIKKPVGFAEDCVGEPAATAIAAMQDGDILCLENTRFHPEEEKNDPTFVAKLAELGDIWVNDAFSAAHRAHASTEGLG
HKLPAYAGRTMQAELVALNKALEAPTKPVIAIVGGAKVSSKIDLLENLVTKVQALVIGGGMANTFLHAQGFKVGKSLCEK
DLAETALRIMNKAEAASCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRSIERIHAAIDDAATLVWNGPVG
AFELTPFDKGTVEAARHAARRTKAGMLISVAGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLRVK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=42.6829268292683, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=41.9512195121951, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=42.8571428571429, Blast_Score=291, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=43.980343980344, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6319857, Length=411, Percent_Identity=40.3892944038929, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=42.6150121065375, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=40.4411764705882, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41988; Mature: 41856

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSFRTLDDVDVKGQRVLLRVDLNVPMDSGKVTDTTRLDRVAPTITEIAGKGGKVILLA
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
HFGRPKGRDDKNSLKPVAAALADVIKKPVGFAEDCVGEPAATAIAAMQDGDILCLENTRF
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHEECCCCEEEEECCCC
HPEEEKNDPTFVAKLAELGDIWVNDAFSAAHRAHASTEGLGHKLPAYAGRTMQAELVALN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHH
KALEAPTKPVIAIVGGAKVSSKIDLLENLVTKVQALVIGGGMANTFLHAQGFKVGKSLCE
HHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
KDLAETALRIMNKAEAASCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRS
HHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCEECCCCCCCCCEEEECCHHH
IERIHAAIDDAATLVWNGPVGAFELTPFDKGTVEAARHAARRTKAGMLISVAGGGDTVAA
HHHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEE
LNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLRVK
ECCCCCCCCCCEEECCCCHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure 
TKSFRTLDDVDVKGQRVLLRVDLNVPMDSGKVTDTTRLDRVAPTITEIAGKGGKVILLA
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
HFGRPKGRDDKNSLKPVAAALADVIKKPVGFAEDCVGEPAATAIAAMQDGDILCLENTRF
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHEECCCCEEEEECCCC
HPEEEKNDPTFVAKLAELGDIWVNDAFSAAHRAHASTEGLGHKLPAYAGRTMQAELVALN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHH
KALEAPTKPVIAIVGGAKVSSKIDLLENLVTKVQALVIGGGMANTFLHAQGFKVGKSLCE
HHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
KDLAETALRIMNKAEAASCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRS
HHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCEECCCCCCCCCEEEECCHHH
IERIHAAIDDAATLVWNGPVGAFELTPFDKGTVEAARHAARRTKAGMLISVAGGGDTVAA
HHHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEE
LNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLRVK
ECCCCCCCCCCEEECCCCHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA