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The map label for this gene is mer [H]

Identifier: 86751532

GI number: 86751532

Start: 5021437

End: 5022402

Strand: Direct

Name: mer [H]

Synonym: RPB_4431

Alternate gene names: 86751532

Gene position: 5021437-5022402 (Clockwise)

Preceding gene: 86751531

Following gene: 86751534

Centisome position: 94.18

GC content: 69.46

Gene sequence:

>966_bases
ATGACCAGCACGCTCGGCCTCTCTTTCGACGGCGGCGAGACCTCCGACTCATTTCGCGCGATGATCGAACTGGGCGATCG
CGGCGGCGCCTCGACGGCCTGGCTCGCCTCGCATTTGTTCCAGCGCGAACCGATCTCCTCGGCTGCGATCGCACTCGGCG
CCACCAGCCGGATCAGCATCGCCCTGATGGCGATGAGCCCGTATTCGGTGCATCCGCTCTACGCCACCATGGCCGCGGCG
ACGCTGGACGAGTATTTTCCCGGCCGCGTCAAACTTTGCTTCGGCGTCGGCGCGCCGCGCGATCTCGAAGCTGCGGGCCT
CGTCGCCGAGCATCCGCTCGGCACCCTGCGCGAGGCGATCGCGCTGTCGCGTGCGCTGCTCGGCGGCGAAACGGTCGATT
TCAAAGGTGAGCGCTTCAAGGTCTCGGGCCGACGGCTGTCGACCGGCGCGCGCGCCGTCCCGATCTATCTGGCCGCCTCG
GGCCCGCAGATGCTCGAACTCGCCGGCGCCGCCGCCGACGGCGTGCTGATCAGCGCGGCGACCTCGCCGGCTTTCATCCG
CTGGACGCTCGATCTCGTCCGCAAGGGCGAAGAGAAGGCCGGCCGGGTCATCAAGAAGACGGCGCTCGTCTATGTTTCGG
CCGATGCCGACGAGACCACCGCCCGCGACCGCCTGCGCCGCACCCTCGGTTTCATCCTGCGCGGCCAGCACCATGCCCGC
AATCTCGAACTCGCGGGCACGAAGCTCGACCAGGCCGCGCTCGCCGCGGCCTATGCGCGCGAAGACTGGGACGCGGTGAA
CGCGCTGGTGACGGACGACGTGGTGATGCGCCACAGCGCCAGCGGCACGCCGGAGCAGGTCCGTGCGGCGTTCGCGGCGT
ATGAGGATGTCGGCGTCGACGAGATCGTGGCGTCCGGCATGGGCACCCCCGCGGAGCTGCGGCAACTCCTCGAGGCGCTC
GAATAG

Upstream 100 bases:

>100_bases
GACGTCCAGGAACTGCTGGACCAGATGCAATTCTTCGCCGAAGAGGTGATGCCGCGGATCGAGTGACACCGCGCATCGCG
CATTTCCGGAAAGGCGCCCC

Downstream 100 bases:

>100_bases
TTGCGTCAGCGCCCGCGGCCGCCGGATGGCGCCGACGGCGCCCCCATCTTGCGCGAGACGCTGTCGGCCGCCGACTTCAC
CAGCGCGTTGACGTGTTCGA

Product: coenzyme F420-dependent N(5),N(10)-methenyltetrahydromethanopterin

Products: NA

Alternate protein names: Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase; Methylene-H(4)MPT reductase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTSTLGLSFDGGETSDSFRAMIELGDRGGASTAWLASHLFQREPISSAAIALGATSRISIALMAMSPYSVHPLYATMAAA
TLDEYFPGRVKLCFGVGAPRDLEAAGLVAEHPLGTLREAIALSRALLGGETVDFKGERFKVSGRRLSTGARAVPIYLAAS
GPQMLELAGAAADGVLISAATSPAFIRWTLDLVRKGEEKAGRVIKKTALVYVSADADETTARDRLRRTLGFILRGQHHAR
NLELAGTKLDQAALAAAYAREDWDAVNALVTDDVVMRHSASGTPEQVRAAFAAYEDVGVDEIVASGMGTPAELRQLLEAL
E

Sequences:

>Translated_321_residues
MTSTLGLSFDGGETSDSFRAMIELGDRGGASTAWLASHLFQREPISSAAIALGATSRISIALMAMSPYSVHPLYATMAAA
TLDEYFPGRVKLCFGVGAPRDLEAAGLVAEHPLGTLREAIALSRALLGGETVDFKGERFKVSGRRLSTGARAVPIYLAAS
GPQMLELAGAAADGVLISAATSPAFIRWTLDLVRKGEEKAGRVIKKTALVYVSADADETTARDRLRRTLGFILRGQHHAR
NLELAGTKLDQAALAAAYAREDWDAVNALVTDDVVMRHSASGTPEQVRAAFAAYEDVGVDEIVASGMGTPAELRQLLEAL
E
>Mature_320_residues
TSTLGLSFDGGETSDSFRAMIELGDRGGASTAWLASHLFQREPISSAAIALGATSRISIALMAMSPYSVHPLYATMAAAT
LDEYFPGRVKLCFGVGAPRDLEAAGLVAEHPLGTLREAIALSRALLGGETVDFKGERFKVSGRRLSTGARAVPIYLAASG
PQMLELAGAAADGVLISAATSPAFIRWTLDLVRKGEEKAGRVIKKTALVYVSADADETTARDRLRRTLGFILRGQHHARN
LELAGTKLDQAALAAAYAREDWDAVNALVTDDVVMRHSASGTPEQVRAAFAAYEDVGVDEIVASGMGTPAELRQLLEALE

Specific function: Catalyzes the oxidation of methyl-H(4)MPT to methylene- H(4)MPT [H]

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mer family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR019946 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: =1.5.99.11 [H]

Molecular weight: Translated: 33974; Mature: 33843

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTLGLSFDGGETSDSFRAMIELGDRGGASTAWLASHLFQREPISSAAIALGATSRISI
CCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHEEECCCCCEEE
ALMAMSPYSVHPLYATMAAATLDEYFPGRVKLCFGVGAPRDLEAAGLVAEHPLGTLREAI
EEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCHHHHHHHH
ALSRALLGGETVDFKGERFKVSGRRLSTGARAVPIYLAASGPQMLELAGAAADGVLISAA
HHHHHHHCCCEECCCCCEEEECCCEECCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEC
TSPAFIRWTLDLVRKGEEKAGRVIKKTALVYVSADADETTARDRLRRTLGFILRGQHHAR
CCCEEEEEEHHHHHCCHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHC
NLELAGTKLDQAALAAAYAREDWDAVNALVTDDVVMRHSASGTPEQVRAAFAAYEDVGVD
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHH
EIVASGMGTPAELRQLLEALE
HHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TSTLGLSFDGGETSDSFRAMIELGDRGGASTAWLASHLFQREPISSAAIALGATSRISI
CCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHEEECCCCCEEE
ALMAMSPYSVHPLYATMAAATLDEYFPGRVKLCFGVGAPRDLEAAGLVAEHPLGTLREAI
EEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCHHHHHHHH
ALSRALLGGETVDFKGERFKVSGRRLSTGARAVPIYLAASGPQMLELAGAAADGVLISAA
HHHHHHHCCCEECCCCCEEEECCCEECCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEC
TSPAFIRWTLDLVRKGEEKAGRVIKKTALVYVSADADETTARDRLRRTLGFILRGQHHAR
CCCEEEEEEHHHHHCCHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHC
NLELAGTKLDQAALAAAYAREDWDAVNALVTDDVVMRHSASGTPEQVRAAFAAYEDVGVD
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHH
EIVASGMGTPAELRQLLEALE
HHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]