Click here to switch to the map view.

The map label for this gene is bigR [H]

Identifier: 86751480

GI number: 86751480

Start: 4960368

End: 4960745

Strand: Direct

Name: bigR [H]

Synonym: RPB_4378

Alternate gene names: 86751480

Gene position: 4960368-4960745 (Clockwise)

Preceding gene: 86751479

Following gene: 86751481

Centisome position: 93.04

GC content: 62.17

Gene sequence:

>378_bases
ATGACATCGATCCTCGACCCGGATATCGAGACCGAATTCCGCGACGGCGCGGAATACTCGCCCGAGCTCGACATGCTGAT
GCGCCGCGCGCGCAAGGCGAGCAGTTTTCTCAAGGCGGTGTCGCACGAGAACCGGCTGCTGCTGCTGTGTCTGCTGGCCG
AGCGCGAGCGCTCGGTGTCCGAACTGGAGAACATCCTGTCGCTGCGGCAGTCGGCGGTGTCGCAGCAGCTCGCCCGACTG
CGCTACGACGGCATGGTCGATACCAGGCGCGACGGCAAGACCATCTACTATAGTCTCGCCAACGACGATGTCCGTCGCGT
GATCTCCGTGATCTATGATATCTTCTGCAGCTCGGCGAGAGGTGGCGAGCAGAAGTAA

Upstream 100 bases:

>100_bases
CTGGGGGCTGCTCGGGACCATGGTCGCGAAGCTCACGCCGCCGCCCGATCCCAAGCACACCTGAAGCCCGACCGGCGCAA
CCCGAGGCCAATTGAACGAA

Downstream 100 bases:

>100_bases
TCCGACCCTCGCCGCAGCGCACAACGGCCCCGCGACCGACGCGGCGCGTGCGCGCTTTCGAGGGAACCCCATGCAGGACA
CGTCCGCCTGGCTGCTCGCG

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 124

Protein sequence:

>125_residues
MTSILDPDIETEFRDGAEYSPELDMLMRRARKASSFLKAVSHENRLLLLCLLAERERSVSELENILSLRQSAVSQQLARL
RYDGMVDTRRDGKTIYYSLANDDVRRVISVIYDIFCSSARGGEQK

Sequences:

>Translated_125_residues
MTSILDPDIETEFRDGAEYSPELDMLMRRARKASSFLKAVSHENRLLLLCLLAERERSVSELENILSLRQSAVSQQLARL
RYDGMVDTRRDGKTIYYSLANDDVRRVISVIYDIFCSSARGGEQK
>Mature_124_residues
TSILDPDIETEFRDGAEYSPELDMLMRRARKASSFLKAVSHENRLLLLCLLAERERSVSELENILSLRQSAVSQQLARLR
YDGMVDTRRDGKTIYYSLANDDVRRVISVIYDIFCSSARGGEQK

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=96, Percent_Identity=41.6666666666667, Blast_Score=82, Evalue=8e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 14336; Mature: 14204

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSILDPDIETEFRDGAEYSPELDMLMRRARKASSFLKAVSHENRLLLLCLLAERERSVS
CCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
ELENILSLRQSAVSQQLARLRYDGMVDTRRDGKTIYYSLANDDVRRVISVIYDIFCSSAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
GGEQK
CCCCC
>Mature Secondary Structure 
TSILDPDIETEFRDGAEYSPELDMLMRRARKASSFLKAVSHENRLLLLCLLAERERSVS
CCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
ELENILSLRQSAVSQQLARLRYDGMVDTRRDGKTIYYSLANDDVRRVISVIYDIFCSSAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
GGEQK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]