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The map label for this gene is mltB [H]

Identifier: 86751309

GI number: 86751309

Start: 4774711

End: 4776087

Strand: Direct

Name: mltB [H]

Synonym: RPB_4203

Alternate gene names: 86751309

Gene position: 4774711-4776087 (Clockwise)

Preceding gene: 86751308

Following gene: 86751310

Centisome position: 89.55

GC content: 67.83

Gene sequence:

>1377_bases
ATGACGTTTGATGGGGTGGCCATGCGGCGGATGAACTTGGTGCAACGACTGGCGAGCAACTGGCGCGGCGCCGTGTTGGC
AGCACTGGCAACTGCCCTGTCCGCCTCCCCCGCTCTCGCCCAGCGCGGCGCGTCCTGCCATGGCGGGCAGAGCTTCGATC
AGTTTCTCGCCGGGCTGAAACAGCAGGCGGTCGCGGCCGGGGTGTCGCAGCGGGCGATCGCGCAGGCTTCGCCGTATCTC
GTCTACGACCAGGGCATCGTCAATCGCGACCGCGGGCAGCGGGTGTTCGGGCAGATCTTCACGGTGTTCGCCGGGCGGAT
GGCGTCGGAGGGCCGGCGCGTCAAGGGCCAGCAGATGATCAAGCAGCACGCGCAGGCCTTCGCGCGCGCCGAGAAGGAAT
ACGGCGTGCCGCCGGCGGTGATCGCCGCATTCTGGGCGCTGGAGAGCGATTTCGGCGCGGTCCAGGGCAACCTCTCGACG
CTGCGCTCGCTGGTGTCGCTGGCCTATGATTGCCGCCGCTCCGAAAAGTTTCAGCAGGAGACCATCGCGGCGCTGAAGAT
CGTCGACCGCGGCGATCTGGCGCCCGCCGAAATGATCGGCTCCTGGGCCGGCGAGCTCGGCCAGACGCAGTTTTTGCCGC
GGCACTATTACGACTACGCAGTCGACTATGACGGCGACGGCCACCGCGACATGCTGCGCAGCCCCGCCGACGTCATCGGC
TCGACCGCGCATTACATCGCGGCCGGGCTGAAATGGCGGCGCGGCGAGCCCTGGCTGCAGGAAGTGCAGGTGCCGGCCAA
CCTTCCCTGGGATCAGGCCGACCTGCTCATCAAGCATCCGCGCGCGCAATGGGCGCAATGGGGCGTGACTTTCGCCGACG
GACGGCCGCTGCCGGGCGACACGCTACAGGCGTCGCTACTGCTGCCGATGGGCCGCCACGGCCCGGCGTTTCTGGCCTAC
GCGAATTTCGCCGCCTATACCGAATGGAATAATTCGCTGATCTATTCGACCACCGCCGGCTATCTGGCCACACGGATCGC
CGGCGCGCCGCCGATGCGCAAGCCGACCGTGCAGATCGCGCAATTGTCGTTCAACGAGATGAAGCAATTGCAACAGTTGC
TGACGCGCGCCGGCTTCGACGTCGGCAAGATCGACGGCATCCTCGGCCAGCAGAGCCGCAGCGCGGTGAAGGCGATGCAG
ATCAAATACGGCCTGCCGGCGGATTCCTGGCCGACCGCCGAACTGCTGACGAAGATGGGCGGAACGGTTACCCCCGCGCC
GTCCGCTTCCACCGAACCGCCGTTCCGGCCGGTCGCGAGACCCGACGCAGCGCCGGCCGAACGGCGCCCCCGCAATCCAC
CGGCGCGGCAGCCGTAG

Upstream 100 bases:

>100_bases
GATCGCGGCGTTGCCCGATCGCCATGCCGGGCTTGACCCGGCAACCCATCCCAACAAGACTCGTCGTCTGATTTGATGGA
TCGCCGGGTCGAGCCCGGCG

Downstream 100 bases:

>100_bases
CGAAATTTCATCTGCGGCGACGTTCCAACTTCCCCCGACTTGCTCTAGGGTCCGGCGCAACAAAACCCGGAGGAAGAACG
TGCGTCATCTTTCCATTGTT

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTFDGVAMRRMNLVQRLASNWRGAVLAALATALSASPALAQRGASCHGGQSFDQFLAGLKQQAVAAGVSQRAIAQASPYL
VYDQGIVNRDRGQRVFGQIFTVFAGRMASEGRRVKGQQMIKQHAQAFARAEKEYGVPPAVIAAFWALESDFGAVQGNLST
LRSLVSLAYDCRRSEKFQQETIAALKIVDRGDLAPAEMIGSWAGELGQTQFLPRHYYDYAVDYDGDGHRDMLRSPADVIG
STAHYIAAGLKWRRGEPWLQEVQVPANLPWDQADLLIKHPRAQWAQWGVTFADGRPLPGDTLQASLLLPMGRHGPAFLAY
ANFAAYTEWNNSLIYSTTAGYLATRIAGAPPMRKPTVQIAQLSFNEMKQLQQLLTRAGFDVGKIDGILGQQSRSAVKAMQ
IKYGLPADSWPTAELLTKMGGTVTPAPSASTEPPFRPVARPDAAPAERRPRNPPARQP

Sequences:

>Translated_458_residues
MTFDGVAMRRMNLVQRLASNWRGAVLAALATALSASPALAQRGASCHGGQSFDQFLAGLKQQAVAAGVSQRAIAQASPYL
VYDQGIVNRDRGQRVFGQIFTVFAGRMASEGRRVKGQQMIKQHAQAFARAEKEYGVPPAVIAAFWALESDFGAVQGNLST
LRSLVSLAYDCRRSEKFQQETIAALKIVDRGDLAPAEMIGSWAGELGQTQFLPRHYYDYAVDYDGDGHRDMLRSPADVIG
STAHYIAAGLKWRRGEPWLQEVQVPANLPWDQADLLIKHPRAQWAQWGVTFADGRPLPGDTLQASLLLPMGRHGPAFLAY
ANFAAYTEWNNSLIYSTTAGYLATRIAGAPPMRKPTVQIAQLSFNEMKQLQQLLTRAGFDVGKIDGILGQQSRSAVKAMQ
IKYGLPADSWPTAELLTKMGGTVTPAPSASTEPPFRPVARPDAAPAERRPRNPPARQP
>Mature_457_residues
TFDGVAMRRMNLVQRLASNWRGAVLAALATALSASPALAQRGASCHGGQSFDQFLAGLKQQAVAAGVSQRAIAQASPYLV
YDQGIVNRDRGQRVFGQIFTVFAGRMASEGRRVKGQQMIKQHAQAFARAEKEYGVPPAVIAAFWALESDFGAVQGNLSTL
RSLVSLAYDCRRSEKFQQETIAALKIVDRGDLAPAEMIGSWAGELGQTQFLPRHYYDYAVDYDGDGHRDMLRSPADVIGS
TAHYIAAGLKWRRGEPWLQEVQVPANLPWDQADLLIKHPRAQWAQWGVTFADGRPLPGDTLQASLLLPMGRHGPAFLAYA
NFAAYTEWNNSLIYSTTAGYLATRIAGAPPMRKPTVQIAQLSFNEMKQLQQLLTRAGFDVGKIDGILGQQSRSAVKAMQI
KYGLPADSWPTAELLTKMGGTVTPAPSASTEPPFRPVARPDAAPAERRPRNPPARQP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=231, Percent_Identity=34.6320346320346, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 49959; Mature: 49828

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFDGVAMRRMNLVQRLASNWRGAVLAALATALSASPALAQRGASCHGGQSFDQFLAGLK
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHH
QQAVAAGVSQRAIAQASPYLVYDQGIVNRDRGQRVFGQIFTVFAGRMASEGRRVKGQQMI
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KQHAQAFARAEKEYGVPPAVIAAFWALESDFGAVQGNLSTLRSLVSLAYDCRRSEKFQQE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TIAALKIVDRGDLAPAEMIGSWAGELGQTQFLPRHYYDYAVDYDGDGHRDMLRSPADVIG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCEEECCCCCCCHHHHCCCHHHHC
STAHYIAAGLKWRRGEPWLQEVQVPANLPWDQADLLIKHPRAQWAQWGVTFADGRPLPGD
CHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHCCCHHHHHHCCCEECCCCCCCCC
TLQASLLLPMGRHGPAFLAYANFAAYTEWNNSLIYSTTAGYLATRIAGAPPMRKPTVQIA
HHHHHHCCCCCCCCCEEEEEHHHHEEEECCCCEEEECHHHHHHHHHCCCCCCCCCCHHHH
QLSFNEMKQLQQLLTRAGFDVGKIDGILGQQSRSAVKAMQIKYGLPADSWPTAELLTKMG
HHCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
GTVTPAPSASTEPPFRPVARPDAAPAERRPRNPPARQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]