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The map label for this gene is rna [C]

Identifier: 86751294

GI number: 86751294

Start: 4760075

End: 4760770

Strand: Reverse

Name: rna [C]

Synonym: RPB_4187

Alternate gene names: 86751294

Gene position: 4760770-4760075 (Counterclockwise)

Preceding gene: 86751295

Following gene: 86751293

Centisome position: 89.29

GC content: 68.39

Gene sequence:

>696_bases
ATGCGCAATCCTATCGTTCGCCGGCGGGCATGGCTCGCCGCTTTGGCCGCTTGCGCTGTCGCATGTCTCGGCACCGCAGC
GCCTGCCCAGGATCGGCGCCAGAACGCCCCCGGCGAGTTCGATTTTTACGTGCTGGCGCTGTCGTGGTCGCCGTCTTATT
GCGAGCAGGCGTCCGAGCGCGGCAATGCCGGGCGCTCGCAGCAGATCCAGTGCGGCGGCCGGCCGTTCTCCTTCGTGGTG
CACGGGCTGTGGCCGCAATACGAGCGCGGCTTTCCGGAATATTGCCAGCGCCCGTCGCCGCGGCTCGACCGGCGGATCAT
GAATTCCATGCTCGACCTGATGCCGGCGCCGGGGCTGATCTACAACGAATGGGACAAGCACGGCACCTGCGCCGGCACAC
CGGCGCGCGATTACTTCGAGAACATCCGCCGGGCGCGCGCGGCGGTGCGGATTCCGCCGGATTTCATCGACGCCAAGGAA
CCCCGGACAGTGACCCCCGCCGAGGTCGAGGAGGCCTTCATCAAGGTCAATCCCGGCTTGAGCGAGTCGGCGATCGCCGT
GATCTGCGACCGCACCCGGTTGACCGAGGTCCGGATCTGCATGAGCAAGGATCTGCAGTTCCGCGCCTGCGCGGAGGTCG
ACCGCCGCGCCTGCCGTCGCGACCGTCTCGAGGTGCCGCCGGTGCGCGGCGGCTGA

Upstream 100 bases:

>100_bases
CGCCCCTGTTTTGGCGCCAGCGCCTTCCACCATTTGCGTTCTTGAAACGTTCCCGATCGCTCGCTAGGCTTTGCTATTCA
CGGCAGCGGGGCCCTTGATC

Downstream 100 bases:

>100_bases
GGCGCCGCGCATGGCGAAGCCCGGGCGGGGCCGGCACCGGTCGGCCCTCGCAGCCGGCGCCAGCCTGGCGCGCGCTGTCT
TGACTTCACGCGCAGCTCCG

Product: ribonuclease T2

Products: 3'- Phosphomononucleotides; 3'-Phosphooligonucleotides [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRNPIVRRRAWLAALAACAVACLGTAAPAQDRRQNAPGEFDFYVLALSWSPSYCEQASERGNAGRSQQIQCGGRPFSFVV
HGLWPQYERGFPEYCQRPSPRLDRRIMNSMLDLMPAPGLIYNEWDKHGTCAGTPARDYFENIRRARAAVRIPPDFIDAKE
PRTVTPAEVEEAFIKVNPGLSESAIAVICDRTRLTEVRICMSKDLQFRACAEVDRRACRRDRLEVPPVRGG

Sequences:

>Translated_231_residues
MRNPIVRRRAWLAALAACAVACLGTAAPAQDRRQNAPGEFDFYVLALSWSPSYCEQASERGNAGRSQQIQCGGRPFSFVV
HGLWPQYERGFPEYCQRPSPRLDRRIMNSMLDLMPAPGLIYNEWDKHGTCAGTPARDYFENIRRARAAVRIPPDFIDAKE
PRTVTPAEVEEAFIKVNPGLSESAIAVICDRTRLTEVRICMSKDLQFRACAEVDRRACRRDRLEVPPVRGG
>Mature_231_residues
MRNPIVRRRAWLAALAACAVACLGTAAPAQDRRQNAPGEFDFYVLALSWSPSYCEQASERGNAGRSQQIQCGGRPFSFVV
HGLWPQYERGFPEYCQRPSPRLDRRIMNSMLDLMPAPGLIYNEWDKHGTCAGTPARDYFENIRRARAAVRIPPDFIDAKE
PRTVTPAEVEEAFIKVNPGLSESAIAVICDRTRLTEVRICMSKDLQFRACAEVDRRACRRDRLEVPPVRGG

Specific function: One Of The Few RNAses That Cleaves The Phosphodiester Bond Between Any Two Nucleotide. Shows A Preference For Cytidylic Or Guanylic Acid. [C]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasmic Protein And Cytoplasm. [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: 3.1.27.6 [C]

Molecular weight: Translated: 26013; Mature: 26013

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00530 RNASE_T2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNPIVRRRAWLAALAACAVACLGTAAPAQDRRQNAPGEFDFYVLALSWSPSYCEQASER
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEEEEECCCHHHHHHHHC
GNAGRSQQIQCGGRPFSFVVHGLWPQYERGFPEYCQRPSPRLDRRIMNSMLDLMPAPGLI
CCCCCCCEEEECCCHHHHHHHCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCE
YNEWDKHGTCAGTPARDYFENIRRARAAVRIPPDFIDAKEPRTVTPAEVEEAFIKVNPGL
ECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHEEECCCC
SESAIAVICDRTRLTEVRICMSKDLQFRACAEVDRRACRRDRLEVPPVRGG
CCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRNPIVRRRAWLAALAACAVACLGTAAPAQDRRQNAPGEFDFYVLALSWSPSYCEQASER
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEEEEECCCHHHHHHHHC
GNAGRSQQIQCGGRPFSFVVHGLWPQYERGFPEYCQRPSPRLDRRIMNSMLDLMPAPGLI
CCCCCCCEEEECCCHHHHHHHCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCE
YNEWDKHGTCAGTPARDYFENIRRARAAVRIPPDFIDAKEPRTVTPAEVEEAFIKVNPGL
ECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHEEECCCC
SESAIAVICDRTRLTEVRICMSKDLQFRACAEVDRRACRRDRLEVPPVRGG
CCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nucleotides [C]

Specific reaction: Endonucleolytic Cleavage With 2',3'-Cyclic Phosphate Intermediates. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]