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The map label for this gene is fadA [H]

Identifier: 86751258

GI number: 86751258

Start: 4726209

End: 4727462

Strand: Reverse

Name: fadA [H]

Synonym: RPB_4151

Alternate gene names: 86751258

Gene position: 4727462-4726209 (Counterclockwise)

Preceding gene: 86751262

Following gene: 86751257

Centisome position: 88.67

GC content: 67.22

Gene sequence:

>1254_bases
ATGGCCGAAGCCTACATCATCGACGCCGTCCGCACCCCCCGCGGCATCGGCAAAGTCGGCAAGGGCAAACTGGCGGAGAT
GCATCCGCAACACCTTGCCGCCGCCGTGCTCAAGGCCATTGCCGAGCGCAACCAGCTCAACACCGCCGAAGTCGACGATA
TCATCTGGTCGACCTCCACCCAGCGCGGTAAGCAGGGCGGCGACCTCGGCCGCATGGCGGCGCTCGATGCGGGCTACGAC
ATCAAGGCCTCCGGCACCACGCTCGACCGTTTCTGCGGCGGCGGCATCACCGCGGTGAATTTCGCCGCGGCCCAGATCAT
GAGCGGCATGGAAGACGTGGTGATCGCCGGCGGCACCGAGATGATGTCGCTGACCGCATCGATGGCCGCCGAGGACATGG
CCGCCGGCAAGCCGCCACTCGGCATGGGCTCGGGCAATGCCCGCCTCGCTCAGGTGCACCCGCAATCGCATCAGGGCATC
TGCGGCGACGCGATCGCCACGATGGAAGGCATCAGCCGCGAGGCGCTCGACGCGCTCGGGCTGGAGAGCCAGCGCCGCGC
CGCGATCGCCATCAAGGAAGGCCGCTTCGACAAGAGCATCATCCCGGTCAAGGACGACGACGGCAACGTCGTGCTGGCGA
AGGACGAATATCCGCGCCCCGAAACCACCGCCGAAGGCCTCGCCGCGCTGAAGCCGGCCTTCACCGCGATCGCCGACTAT
CCGCTCGACGACAAGGGCACCACCTATCGCAAGCTGATCAACCAGAAGTATCCGGACGTCGACATCAAGCACGTCCACCA
CGCCGGCAATTCCTCGGGCGTGGTCGACGGCGCCGCCGCGGTGCTGCTGACCTCGAAGGCCTATGCCGACGCCCACGGCC
TCAAGCCGCGCGCCAAGATCGTGGCGATGGCCAATATCGGCGACGACCCGACGCTGATGCTGAACGCGCCGGTGCCGGCG
GCCAAGAAGGTGCTGGCCAAGGCCGGACTCACCAAGGACGATATCGACCTCTGGGAGATCAACGAAGCCTTCGCCGTGGT
CACCGAGAAATTCATCCGCGACCTCGACCTCGACCGTGACAAGGTCAACGTCAATGGCGGCTCGATCGCCCTCGGCCACC
CGATCGGCGCCACCGGCGCGATCCTGATCGGCACCGTGCTCGACGAACTGGAGCGCCGCGGCCTGAAGCGCGGCCTCGTC
ACGATGTGCGCCGCCGGCGGCATGGCCCCGGCGATCATCATCGAGCGGGTGTGA

Upstream 100 bases:

>100_bases
GAATGGCAATTCGAGGCGCGTGCGCAGCGGGCTTGACTTCGCACCCGGCTCGGACAATCTCGCCGCCAATCAATCTCAAG
AACCCTGGGAGAACAAGAAA

Downstream 100 bases:

>100_bases
GGCGAGGGGCGGCTTCGCACGGCGCGGCGGCATTGCGTGGTCCGCGCGGCGTTGACGAACTGAACTGACTGAAAAGGGCG
GCGCCGCACGCGCTGCCCTT

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MAEAYIIDAVRTPRGIGKVGKGKLAEMHPQHLAAAVLKAIAERNQLNTAEVDDIIWSTSTQRGKQGGDLGRMAALDAGYD
IKASGTTLDRFCGGGITAVNFAAAQIMSGMEDVVIAGGTEMMSLTASMAAEDMAAGKPPLGMGSGNARLAQVHPQSHQGI
CGDAIATMEGISREALDALGLESQRRAAIAIKEGRFDKSIIPVKDDDGNVVLAKDEYPRPETTAEGLAALKPAFTAIADY
PLDDKGTTYRKLINQKYPDVDIKHVHHAGNSSGVVDGAAAVLLTSKAYADAHGLKPRAKIVAMANIGDDPTLMLNAPVPA
AKKVLAKAGLTKDDIDLWEINEAFAVVTEKFIRDLDLDRDKVNVNGGSIALGHPIGATGAILIGTVLDELERRGLKRGLV
TMCAAGGMAPAIIIERV

Sequences:

>Translated_417_residues
MAEAYIIDAVRTPRGIGKVGKGKLAEMHPQHLAAAVLKAIAERNQLNTAEVDDIIWSTSTQRGKQGGDLGRMAALDAGYD
IKASGTTLDRFCGGGITAVNFAAAQIMSGMEDVVIAGGTEMMSLTASMAAEDMAAGKPPLGMGSGNARLAQVHPQSHQGI
CGDAIATMEGISREALDALGLESQRRAAIAIKEGRFDKSIIPVKDDDGNVVLAKDEYPRPETTAEGLAALKPAFTAIADY
PLDDKGTTYRKLINQKYPDVDIKHVHHAGNSSGVVDGAAAVLLTSKAYADAHGLKPRAKIVAMANIGDDPTLMLNAPVPA
AKKVLAKAGLTKDDIDLWEINEAFAVVTEKFIRDLDLDRDKVNVNGGSIALGHPIGATGAILIGTVLDELERRGLKRGLV
TMCAAGGMAPAIIIERV
>Mature_416_residues
AEAYIIDAVRTPRGIGKVGKGKLAEMHPQHLAAAVLKAIAERNQLNTAEVDDIIWSTSTQRGKQGGDLGRMAALDAGYDI
KASGTTLDRFCGGGITAVNFAAAQIMSGMEDVVIAGGTEMMSLTASMAAEDMAAGKPPLGMGSGNARLAQVHPQSHQGIC
GDAIATMEGISREALDALGLESQRRAAIAIKEGRFDKSIIPVKDDDGNVVLAKDEYPRPETTAEGLAALKPAFTAIADYP
LDDKGTTYRKLINQKYPDVDIKHVHHAGNSSGVVDGAAAVLLTSKAYADAHGLKPRAKIVAMANIGDDPTLMLNAPVPAA
KKVLAKAGLTKDDIDLWEINEAFAVVTEKFIRDLDLDRDKVNVNGGSIALGHPIGATGAILIGTVLDELERRGLKRGLVT
MCAAGGMAPAIIIERV

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=415, Percent_Identity=34.6987951807229, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI4501853, Length=426, Percent_Identity=34.9765258215962, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI167614485, Length=418, Percent_Identity=33.7320574162679, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI4557237, Length=429, Percent_Identity=31.7016317016317, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4504327, Length=447, Percent_Identity=31.0961968680089, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI194353979, Length=416, Percent_Identity=25.9615384615385, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1787663, Length=430, Percent_Identity=39.5348837209302, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI48994986, Length=433, Percent_Identity=36.2586605080831, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788554, Length=427, Percent_Identity=37.9391100702576, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI87082165, Length=427, Percent_Identity=35.1288056206089, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788683, Length=465, Percent_Identity=26.8817204301075, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI133906874, Length=419, Percent_Identity=33.4128878281623, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25147385, Length=422, Percent_Identity=30.0947867298578, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=422, Percent_Identity=28.9099526066351, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17551802, Length=455, Percent_Identity=28.7912087912088, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17535917, Length=425, Percent_Identity=27.7647058823529, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322031, Length=347, Percent_Identity=35.1585014409222, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=425, Percent_Identity=31.5294117647059, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24655093, Length=426, Percent_Identity=36.6197183098592, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17648125, Length=381, Percent_Identity=34.3832020997375, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=32.3943661971831, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17137578, Length=449, Percent_Identity=29.3986636971047, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 43702; Mature: 43570

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYIIDAVRTPRGIGKVGKGKLAEMHPQHLAAAVLKAIAERNQLNTAEVDDIIWSTST
CCCEEEEECHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
QRGKQGGDLGRMAALDAGYDIKASGTTLDRFCGGGITAVNFAAAQIMSGMEDVVIAGGTE
CCCCCCCCCHHHHEECCCCEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCHH
MMSLTASMAAEDMAAGKPPLGMGSGNARLAQVHPQSHQGICGDAIATMEGISREALDALG
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHC
LESQRRAAIAIKEGRFDKSIIPVKDDDGNVVLAKDEYPRPETTAEGLAALKPAFTAIADY
CCCCCCEEEEEECCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
PLDDKGTTYRKLINQKYPDVDIKHVHHAGNSSGVVDGAAAVLLTSKAYADAHGLKPRAKI
CCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHEEEEEHHHHHHHCCCCCCCEE
VAMANIGDDPTLMLNAPVPAAKKVLAKAGLTKDDIDLWEINEAFAVVTEKFIRDLDLDRD
EEEEECCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCC
KVNVNGGSIALGHPIGATGAILIGTVLDELERRGLKRGLVTMCAAGGMAPAIIIERV
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
AEAYIIDAVRTPRGIGKVGKGKLAEMHPQHLAAAVLKAIAERNQLNTAEVDDIIWSTST
CCEEEEECHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
QRGKQGGDLGRMAALDAGYDIKASGTTLDRFCGGGITAVNFAAAQIMSGMEDVVIAGGTE
CCCCCCCCCHHHHEECCCCEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCHH
MMSLTASMAAEDMAAGKPPLGMGSGNARLAQVHPQSHQGICGDAIATMEGISREALDALG
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHC
LESQRRAAIAIKEGRFDKSIIPVKDDDGNVVLAKDEYPRPETTAEGLAALKPAFTAIADY
CCCCCCEEEEEECCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
PLDDKGTTYRKLINQKYPDVDIKHVHHAGNSSGVVDGAAAVLLTSKAYADAHGLKPRAKI
CCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHEEEEEHHHHHHHCCCCCCCEE
VAMANIGDDPTLMLNAPVPAAKKVLAKAGLTKDDIDLWEINEAFAVVTEKFIRDLDLDRD
EEEEECCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCC
KVNVNGGSIALGHPIGATGAILIGTVLDELERRGLKRGLVTMCAAGGMAPAIIIERV
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]