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The map label for this gene is vapI [H]

Identifier: 86751255

GI number: 86751255

Start: 4724992

End: 4725297

Strand: Direct

Name: vapI [H]

Synonym: RPB_4148

Alternate gene names: 86751255

Gene position: 4724992-4725297 (Clockwise)

Preceding gene: 86751254

Following gene: 86751256

Centisome position: 88.62

GC content: 65.69

Gene sequence:

>306_bases
ATGGCCACACCAAAGCAGCTCCCCCCGATCCCTCCCGGCGAGATTCTGATCGAGGAATTCATGCGTCCGAACGGCATCAG
CCAAAATAGGCTGGCACGCGATATCGACATCAACCCGGCCCGGGTCAACGACATCGTGCACGGCCGCTCTGCGATCACCG
CTTCCGTCGCCCTGCGGCTGGCGAAGTATTTCGGCACGACGCCAGAACTGTGGATGAACCTGCAGGCCTCCTACGATCTG
CGCCGCGCCCGCGCCGCAGACTGGCCCGCGATCGAGCCGCGCGTCCGCGTGCTCGCCGCGGAGTAG

Upstream 100 bases:

>100_bases
ACTCGATCCGCATCAACGATCAATGGCGGATCTGCTTCGAATGGCGCGACGACGGCGCAGAGATGGTCGAGATTTTGGAC
TATCACTAGAGGCGGTCAGG

Downstream 100 bases:

>100_bases
ACGGGCTGCGGAAGCGACTGGCCCGGGAGTTGGAGGATTGAACATGGTGAGCACGACGCCGCTCGACGTGGCCGACTATC
TCGAGAGTCCGGAGATGATC

Product: XRE family plasmid maintenance system antidote protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 101; Mature: 100

Protein sequence:

>101_residues
MATPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITASVALRLAKYFGTTPELWMNLQASYDL
RRARAADWPAIEPRVRVLAAE

Sequences:

>Translated_101_residues
MATPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITASVALRLAKYFGTTPELWMNLQASYDL
RRARAADWPAIEPRVRVLAAE
>Mature_100_residues
ATPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITASVALRLAKYFGTTPELWMNLQASYDLR
RARAADWPAIEPRVRVLAAE

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081745, Length=73, Percent_Identity=36.986301369863, Blast_Score=61, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 11301; Mature: 11170

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITASVALRL
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
AKYFGTTPELWMNLQASYDLRRARAADWPAIEPRVRVLAAE
HHHHCCCHHHHEECCCCCHHHHHCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
ATPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITASVALRL
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
AKYFGTTPELWMNLQASYDLRRARAADWPAIEPRVRVLAAE
HHHHCCCHHHHEECCCCCHHHHHCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]