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The map label for this gene is carA [H]

Identifier: 86751249

GI number: 86751249

Start: 4716515

End: 4717708

Strand: Direct

Name: carA [H]

Synonym: RPB_4142

Alternate gene names: 86751249

Gene position: 4716515-4717708 (Clockwise)

Preceding gene: 86751246

Following gene: 86751253

Centisome position: 88.46

GC content: 66.58

Gene sequence:

>1194_bases
ATGACCAATTCAGCATCCAATCCCGCCTGGCCGGACCACAAACCGACCGCGCTGCTCGTGCTCGCCGATGGCACCGTGTT
CGAGGGCTTCGGCCTCGGCGCGGAGGGCCACGCCGTCGGCGAGGTCTGCTTCAACACCGCGATGACCGGCTATGAGGAGA
TCCTCACCGACCCGTCCTATGCCGGCCAGCTCATCACCTTCACCTTCCCGCATATCGGCAATGTCGGCACCAACGACGAG
GATATCGAGACGGTGAACATGGCGGCGACGCCGGGCGCGCGCGGCGTGATCCTGCGCGACGCCATCACCGACCCGTCGAA
CTATCGCTCGTCGCGGCATCTCGACGGCTGGCTGAAAGCGCGCGGCATCATCGGCCTGTCGGGCATCGACACCCGCGCCC
TGACCGCGCTGATCCGCGACAAGGGCATGCCGAATGCGGTGATCGCCCATGCGCCGGACGGCAAGTTCGACTTGCACGCG
CTGAAGGAAGAAGCCCGCGAATGGCCCGGCCTCGAAGGCATGGACCTGGTGCCGATGGTGACCTCGGCGCAGCGCTTCAG
CTGGGACGAGACGCCGTGGGCCTGGGGCGAAGGCTTCGGCCGGCAGGACAATCCGGAATTCCACGTCGTGGCGATCGACT
ACGGCGTCAAGCGCAACATCCTGCGGCTGCTCGCCGGCGAAGGCTGCAAGGTCACGGTGGTGCCGGCGACCACCTCGGCC
GACGACATCCTGGCGCTGAAGCCGGACGGCGTGTTCCTGTCGAACGGCCCGGGCGACCCGGCGGCAACCGGCAAATACGC
GGTGCCGGTGATCCAGCAGGTGATCAGTTCCGGCGTGCCGACCTTCGGCATTTGTCTCGGCCACCAGATGCTCGGCCTCG
CGCTCGGCGGCAAGACCGTGAAGATGCATCAGGGCCACCACGGCGCCAATCATCCGGTCAAGGATCTCACCACCGGCAAG
GTCGAGATCACCTCGATGAACCACGGCTTCGCGGTCGACAAGACCACGCTGCCGGCCAATGTGCAGCAGACCCACGTGTC
GCTGTTCGACGACAGCAATTGCGGCATCGCGCTGTCCGACCGGCCGGTGTTCTCGGTGCAGTACCACCCGGAAGCCTCGC
CGGGCCCGCGCGACTCGCATTATCTGTTCCGCCGGTTCTCGGACCTGATGCGGGCGAAGAAGAGCGCGGCGTAA

Upstream 100 bases:

>100_bases
TAGACAGGGCAACATCCCTGTCAAATCTCGTCGAAAACCGAACGAAATGCCCTTCCCGGCGCTTTGACGTCAGCAAAGCC
TCGGACTATGTAATGCGCTC

Downstream 100 bases:

>100_bases
GACGGTTCCTGGGCTGATGGGCTAGCGCCTACAGCGTCGTCATCCTGAGGTGCGCGCCCTTGCGCGCCTCGAAGGATGCG
GAGGCAGGAATTGCCGCGCG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTNSASNPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDE
DIETVNMAATPGARGVILRDAITDPSNYRSSRHLDGWLKARGIIGLSGIDTRALTALIRDKGMPNAVIAHAPDGKFDLHA
LKEEAREWPGLEGMDLVPMVTSAQRFSWDETPWAWGEGFGRQDNPEFHVVAIDYGVKRNILRLLAGEGCKVTVVPATTSA
DDILALKPDGVFLSNGPGDPAATGKYAVPVIQQVISSGVPTFGICLGHQMLGLALGGKTVKMHQGHHGANHPVKDLTTGK
VEITSMNHGFAVDKTTLPANVQQTHVSLFDDSNCGIALSDRPVFSVQYHPEASPGPRDSHYLFRRFSDLMRAKKSAA

Sequences:

>Translated_397_residues
MTNSASNPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDE
DIETVNMAATPGARGVILRDAITDPSNYRSSRHLDGWLKARGIIGLSGIDTRALTALIRDKGMPNAVIAHAPDGKFDLHA
LKEEAREWPGLEGMDLVPMVTSAQRFSWDETPWAWGEGFGRQDNPEFHVVAIDYGVKRNILRLLAGEGCKVTVVPATTSA
DDILALKPDGVFLSNGPGDPAATGKYAVPVIQQVISSGVPTFGICLGHQMLGLALGGKTVKMHQGHHGANHPVKDLTTGK
VEITSMNHGFAVDKTTLPANVQQTHVSLFDDSNCGIALSDRPVFSVQYHPEASPGPRDSHYLFRRFSDLMRAKKSAA
>Mature_396_residues
TNSASNPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDED
IETVNMAATPGARGVILRDAITDPSNYRSSRHLDGWLKARGIIGLSGIDTRALTALIRDKGMPNAVIAHAPDGKFDLHAL
KEEAREWPGLEGMDLVPMVTSAQRFSWDETPWAWGEGFGRQDNPEFHVVAIDYGVKRNILRLLAGEGCKVTVVPATTSAD
DILALKPDGVFLSNGPGDPAATGKYAVPVIQQVISSGVPTFGICLGHQMLGLALGGKTVKMHQGHHGANHPVKDLTTGKV
EITSMNHGFAVDKTTLPANVQQTHVSLFDDSNCGIALSDRPVFSVQYHPEASPGPRDSHYLFRRFSDLMRAKKSAA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=37.17277486911, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI21361331, Length=416, Percent_Identity=33.4134615384615, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI169790915, Length=416, Percent_Identity=33.4134615384615, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1786215, Length=387, Percent_Identity=52.7131782945737, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI193204318, Length=390, Percent_Identity=37.6923076923077, Blast_Score=224, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=34.8600508905852, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324878, Length=382, Percent_Identity=35.3403141361257, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=34.8717948717949, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24642586, Length=390, Percent_Identity=34.8717948717949, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42541; Mature: 42410

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSASNPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSY
CCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRDAITDPSNYRSSRHLDGWLKA
CCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHCCCCCCCCHHHHH
RGIIGLSGIDTRALTALIRDKGMPNAVIAHAPDGKFDLHALKEEAREWPGLEGMDLVPMV
CCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
TSAQRFSWDETPWAWGEGFGRQDNPEFHVVAIDYGVKRNILRLLAGEGCKVTVVPATTSA
HCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCC
DDILALKPDGVFLSNGPGDPAATGKYAVPVIQQVISSGVPTFGICLGHQMLGLALGGKTV
CCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHEECCCEE
KMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDKTTLPANVQQTHVSLFDDSNCGIALSD
EEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCEEEEEEEECCCCCEEECC
RPVFSVQYHPEASPGPRDSHYLFRRFSDLMRAKKSAA
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNSASNPAWPDHKPTALLVLADGTVFEGFGLGAEGHAVGEVCFNTAMTGYEEILTDPSY
CCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRDAITDPSNYRSSRHLDGWLKA
CCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHCCCCCCCCHHHHH
RGIIGLSGIDTRALTALIRDKGMPNAVIAHAPDGKFDLHALKEEAREWPGLEGMDLVPMV
CCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
TSAQRFSWDETPWAWGEGFGRQDNPEFHVVAIDYGVKRNILRLLAGEGCKVTVVPATTSA
HCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCC
DDILALKPDGVFLSNGPGDPAATGKYAVPVIQQVISSGVPTFGICLGHQMLGLALGGKTV
CCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHEECCCEE
KMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDKTTLPANVQQTHVSLFDDSNCGIALSD
EEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCEEEEEEEECCCCCEEECC
RPVFSVQYHPEASPGPRDSHYLFRRFSDLMRAKKSAA
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]